Diagenode

True MicroChIP Kit

ChIP kit icon

Catalog Number
Format
Price
C01010130
(AB-002-0016)
16 rxns
$510.00

The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This microChIP-seq assay has been validated with the new generation IP-Star® Compact Automated Workstation. Furthermore Diagenode's high quality ChIP-seq grade antibodies have been used. 

Video article

Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells

Jove logo

  • Characteristics
    • Revolutionary: Only 10,000 cells needed for complete ChIP-seq procedure
    • Reliable and accurate sequencing library preparation from just picogram inputs
    • Validated on studies for histone marks and with the IP-Star® Compact Automated Platform

    ChIP on 10,000 cells

    High efficiency ChIP on 10,000 cells
    ChIP efficiency on 10,000 cells. ChIP was performed on human Hela cells using the Diagenode antibodies H3K4me3 (Cat. No. pAb-003-050), H3K27ac (pAb-174-050), H3K9me3 (pAb-056-050) and H3K27me3 (pAb-069-050). Sheared chromatin from 10,000 cells and 0.1 µg (H3K27ac), 0.25 µg (H3K4me3 and H3K27me3) or 0.5 µg (H3K9me3) of the antibody were used per IP. Corresponding amount of IgG was used as control. Quantitative PCR was performed with primers for corresponding positive and negative loci. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP amplification

    Match between True MicroChIP peaks and the reference dataset

    Reliable detection of enrichments in ChIP-seq
    A: ChIP has been peformed with H3K4me3 antibody, amplification of 17 pg of DNA ChIP'd from 10,000 cells and amplification of 35 pg of DNA ChIP'd from 100,000 cells (control experiment). The IP'd DNA was amplified and transformed into a sequencing-ready preparation for the Illumina plateform with the MicroPlex Library Preparation kit. The library was then analysed on an Illumina® Genome Analyzer. Cluster generation and sequencing were performed according to the manufacturer's instructions.
    B: We observed a perfect match between the top 40% of True MicroChIP peaks and the reference dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset.

    Cell lysis

    Hela cells were fixed with 1% formaldehyde (for 10 minutes at RT)
    Cell lysis was performed using the Lysis Buffer tL1 of the Diagenode True MicroChIP kit. Samples corresponding to 10,000 cells are sheared during 5 rounds of 5 cycles of 30 seconds “ON” / 30 seconds “OFF” with the Bioruptor® Plus combined with the Bioruptor® Water cooler (Cat No. BioAcc-cool) at HIGH power setting (position H). For optimal results, samples are vortexed before and after performing 5 sonication cycles, followed by a short centrifugation at 4°C. 10 μl of DNA (equivalent to 60,000 cells) are analysed on a 1.5% agarose gel.

  •  Testimonials

    I am working with the True MicroChIP & Microplex Library Preparation Kits and several histone modification antibodies like H3K27ac, H3K4me3, H3K36me3, and H3K27me3. I got always very good and reproducible results for my ChIP-seq experiments.

    Andrea Thiesen, ZMB, Developmental Biology, Prof. Dr. Andrea Vortkamp´s lab, University Duisburg-Essen, Germany
  •  Applications
    ChIP-seq
    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on ... Read more
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
  •  Documents
    TrueMicroChIP kit MANUAL
    Diagenode provides the new True MicroChIP kit with optimized reagents and protocol to ena...
    Download
    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    Download
    True MicroChIP and MicroPlex kits APPLICATION NOTE
    From minuscule amounts to magnificent results: reliable ChIP-seq data from 10,000 cells with the ...
    Download
    ChIP kit results with True MicroChIP kit POSTER
    Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become the g...
    Download
    Application note: Best Workflow Practices for ChIP-seq Analysis with Small Samples APPLICATION NOTE
    Combined chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) has become the g...
    Download
    Chromatin shearing with the Diagenode One APPLICATION NOTE
     The Diagenode One is a new affordable solution for Chromatin shearing in ChIP analysis for ...
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: True MicroChIP Kit (Diagenode Cat# C01010130). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  Related products

Events

  • ASHG 2018
    San Diego
    Oct 16-Oct 20, 2018
 See all events

Applications

 See all applications



The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics