Chromatin consists of DNA, histones and non-histone proteins. In forming chromatin, DNA is tightly wrapped around histones. Generally, more highly condensed chromatin is less accessible to transcription factors and other DNA binding proteins to access DNA, which has consequences for gene expression. Understanding the roles of histones and transcription factors is critical in understanding the regulation of gene expression.
Using ChIP-seq analysis, it is possible to profile histone modifications and transcription factor binding genome-wide to understand the regulation of gene expression in disease or in response to a drug treatment. Diagenode’s Epigenomic Profiling Services offer a wide variety of chromatin analysis options through ChIP-seq and ATAC-seq.
Post-translational modification of histones is implicated in the regulation of gene expression, necessitating the study of regulatory elements and their interacting proteins like active promoter and enhancer analysis. Profile genome-wide histone modifications by ChIP-seq analysis to understand transcriptional regulation
Explore the effects of transcription factor binding through ChIP-seq analysis of a multitude of TFs including:
Study genome-wide chromatin accessibility. Identify open chromatin sites and active regulatory elements such as promoter, enhancers, and insulators.