Chromatin consists of DNA, histones and non-histone proteins. In forming chromatin, DNA is tightly wrapped around histones. Generally, more highly condensed chromatin is less accessible to transcription factors and other DNA binding proteins to access DNA, which has consequences for gene expression. Understanding the roles of histones and transcription factors is critical in understanding the regulation of gene expression.
Using ChIP-seq analysis, it is possible to profile histone modifications and transcription factor binding genome-wide to understand the regulation of gene expression in disease or in response to a drug treatment. Diagenode’s Epigenomic Profiling Services offer a wide variety of chromatin analysis options through ChIP-seq and ATAC-seq.