Ideal for low input samples including patient samples
Proven to detect more transcripts than any other commercial method
Full range of RNA-seq services including the quantification of known transcripts:
small RNA-seq (including small non-coding RNA like miRNA)
mRNA-seq
Long non-coding RNA-seq
Whole transcriptome sequencing
Plus, we offer unique and comprehensive RNA-seq analyses:
Alignment to reference genome and quantification of known genes and/or transcripts
Differential expression: differential expression testing between samples for genes and/or transcripts.
Gene ontology terms analysis: analysis of differentially expressed gene sets. Gene Ontology terms that are overrepresented in differentially expressed genes may indicate the underlying biological processes involved.
Pathway analysis: identification of biochemical pathways in which genes associated with differential expression may be overrepresented.
Unique CATS RNA-seq library preparation provides you with exceptional results
Consistency and wide representation of the transcriptome
All detected transcripts in CATS mRNA-seq vs NEBNext Ultra I directional library preparation Kit. No threshold has been set for the analysis. Input: 1 ng poly(A) RNA from human universal total RNA (Agilent, 740000). 36,245 transcripts found in both libraries.
Broad overview of the transcriptome
Biotypes* represented in the libraries CATS mRNA-seq vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2. Input: 1 ng, 10 ng poly(A) RNA from human universal total RNA (Agilent, 740000).
* < 0.04% of total detected transcripts at this threshold is rRNA
Ultra low inputs, excellent reproducibility
Correlation between 2 different operators using CATS small RNA-seq Kit on two different inputs (100 pg vs 1 ng) for ncRNA detected at TPM ≥2 R2=0.86
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