Diagenode

>>>   Click for Diagenode’s approach to COVID-19

Small RNA-seq Service

default alt
  •  Description
  •  Documents
  •  Related
  •   Get a quote
Catalog Number
Format
G02130000
Custom

Small RNA-seq is the method of choice to investigate the regulatory network of small non-coding RNAs (<200 nt length), including but not limited to miRNA transcripts. It utilizes the proprietary technology D-Plex which ensures realistic representation of diverse small non-coding RNA transcripts with minimized bias. This approach is optimal for finding biomarkers from known transcripts and discover new RNA features.

Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of small RNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on a template-switching technology to produce Illumina compatible libraries enriched with the large variety of small non-coding RNAs.

High transcript diversity with small RNA-seq

  • Realistic representation of all small non-coding RNAs
  • Detection of novel transcripts and differentially-expressed small RNAs
  • Investigation of post-transcriptional regulation of gene expression
  • Leveraging the D-Plex technology
  • Working with ultra-low RNA inputs
  • Compatible with biological fluids

See our other RNA-seq Profiling Services

  • Sample Requirements
    • Project size: no minimum
    • Sample type: total isolated RNA or small enriched RNA
    • Required quantities: minimum 100 pg of total RNA or 10 pg of small RNA
    • Shipment conditions: shipped in 1.5 mL or 0.5 mL tubes on dry ice
  • Services Workflow
    Small RNA-seq Service

    What our service includes

    1. RNA quality control
      • Determination of RIN value (RNA integration number)
      • Measurement of RNA concentration
    2. Library preparation
      • Capture and amplification by tailing and switching
      • Library construction
      • Quality control of libraries prior to sequencing
    3. Sequencing on Illumina platforms
      • 10-20 million raw reads per sample
    4. Data analysis (optional for additional fee)
      • Standard bioinformatic analysis
      • Advanced bioinformatic analysis
      • Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request
  • Bioinformatics Analysis

    Standard bioinformatic analysis
    (optional for additional fee)

    Advanced bioinformatic analysis
    (optional for additional fee)

    • Demultiplexing, read quality control, trimming and filtering
    • Alignment to reference genome
    • Quantification of known transcripts

    Provided files:

    • Report with sequencing statistics
    • Raw data in FASTQ format
    • FastQC report
    • Alignment in BAM format
    • Un-normalized read counts
    • Differential expression analysis
    • Gene ontology terms analysis
    • Pathway analysis

  •  Documents
    The Diagenode Epigenetics custom service POSTER
    Complete workflows for genome-scale DNA methylation and histone marks analysis Epigenetics is cr...
    Download
    Epigenomics Profiling Services FLYER
    Chromatin analysis DNA methylation services RNA-seq analysis
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Small RNA-seq Service (Diagenode Cat# G02130000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  Related products

Events

  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
  • Dutch Chromatin Meeting
    Radboud UMC, Geert Grooteplein 15, 6525 EZ Nijmegen, The Netherlands, Experience center, route 292 to 307
    Nov 12, 2021
 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics