| RNA quality control |
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| Library preparation |
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| Sequencing |
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Heatmap clustering of the top 50 differentially expressed microRNAs(miRNAs) after library preparation:
Prior library preparation, exosomes were isolated with Diagenode exosome capture solutions Exosomes
Transcripts detected in miRNA samples of CATS small RNA-seq Kit vs Competitor Kit.
TPM >1 and TPM>10
Input: 1 ng circulating small RNA in plasma samples.
PCR cycles: CATS 14x vs Competitor 21x. Final library yield: CATS 27 ng vs Competitor 1.2 ng.
Transcripts detected in miRNA samples of CATS small RNA-seq Kit vs Competitor Kit.
Input: 1 ng circulating small RNA in plasma samples.
PCR cycles: CATS 14x vs Competitor 21x. Final library yield: CATS 27 ng vs Competitor 1.2 ng.
Representation of the library preparation yield according to different methods (CATS small RNA-seq Kit vs NEBNext UltraI kit) for 100 ng total RNA. Number of PCR cycles applied following manufacturer’s instructions.
Comparison of the number of detected small non-coding RNAs CATS small RNA-seq Kit vs SMARTer
Input: 100 pg isolated small RNA. TPM ≥2. Transcripts taken into consideration: miRNA, piRNA, scRNA, scaRNA, snoRNA, snRNA and vaultRNA. 1,217 transcripts found in both libraries at this expression level.
Transcripts detected in two technical replicates of CATS v2 total RNA-seq libraries. TPM≥2
Input: 1 ng rRNA(-) RNA from human universal total RNA (Agilent, 740000).
Biotypes* represented in the libraries CATS v2 mRNA-seq Kit vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2.
Input: 1 ng, 10 ng poly(A) RNA from human universal total RNA (Agilent, 740000). * < 0.04% of total detected transcripts at this threshold is rRNA
Biotypes* represented in the libraries CATS v2 total RNA-seq Kit vs NEBNext Ultra I directional RNA-seq Kit. TPM ≥2.
Input: 1 ng, 10 ng rRNA(-) RNA from human universal total RNA (Agilent, 740000). * < 2% of total detected transcripts at this threshold is rRNA
All detected transcripts in CATS v2 mRNA-seq Kit vs NEBNext Ultra I directional library preparation Kit. No threshold has been set for the analysis.
Input: 1 ng poly(A) RNA from human universal total RNA (Agilent, 740000). 36,245 transcripts found in both libraries.
 How to properly cite this product in your workDiagenode strongly recommends using this: RNA-seq services (Diagenode Cat# G02030000). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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