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Total RNA-seq Service

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  •  Description
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Catalog Number
Format
G02030000
Custom

Exploring the whole transcriptome allows to gain insight of potential transcriptional and regulatory network mechanisms. With Total RNA-seq services, we will sequence the whole transcriptome and record the complete set of RNA transcripts, including mRNAs and long non-coding RNAs, present in a biological sample under specific circumstances. This approach is optimal for finding biomarkers from known transcripts and discover new RNA features.

Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of RNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation can be performed with an optional rRNA depletion step to enable cost-effective sequencing of RNA samples.

High discovery power with total RNA-seq

  • Detection of both coding and non-coding RNAs with optional rRNA depletion
  • Recognition of long coding RNAs involved in chromatin remodeling and transcriptional/post-transcriptional regulation
  • Investigation of the structure of genes, their splicing patterns and other post transcriptional modifications
  • Suitable for a broad range of input amounts
  • Compatible with FFPE samples
  • Fast turnaround time (from 5 to 6 weeks)

  • Sample Requirements
    • Project size: minimum of 24 samples
    • Sample type: total isolated RNA
    • Required quantities: minimum 5 ng of total RNA (Human, Mouse, Rat) or 100 ng (Plant)
    • Shipment conditions: shipped in 1.5 mL or 0.5 mL tubes on dry ice
  • Services Workflow

    What our service includes

    1. RNA quality control
      • Determination of RIN value (RNA integration number)
      • Measurement of RNA concentration
    2. Library preparation
      • Optional rRNA depletion
      • Stranded library construction
      • Quality control of libraries prior to sequencing
    3. Sequencing on Illumina platforms
      • 50 million raw reads per sample
    4. Data analysis (optional for additional fee)
      • Standard bioinformatic analysis
      • Advanced bioinformatic analysis
      • Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request
  • Bioinformatics Analysis

    Standard bioinformatic analysis
    (optional for additional fee)

    Advanced bioinformatic analysis
    (optional for additional fee)

    • Demultiplexing, read quality control, trimming and filtering
    • Alignment to reference genome
    • Quantification of known transcripts

    Provided files:

    • Report with sequencing statistics
    • Raw data in FASTQ format
    • FastQC report
    • Alignment in BAM format
    • Un-normalized read counts
    • Differential expression analysis
    • Gene ontology terms analysis
    • Pathway analysis
    • Alternative splicing analysis
    • Novel transcript identification

  •  Documents
    The Diagenode Epigenetics custom service POSTER
    Complete workflows for genome-scale DNA methylation and histone marks analysis Epigenetics is cr...
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    Epigenomics Profiling Services FLYER
    Chromatin analysis DNA methylation services RNA-seq analysis
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  •  Publications

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