Diagenode

RNA-seq services

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  •  描述
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目录号
格式
G02030000
Custom

RNA sequencing uses next-generation sequencing (NGS) to uncover the presence and quantity of RNA for gene expression profiling across the transcriptome. Diagenode specializes in generating RNA-seq libraries which is optimal for low or degraded inputs including those from liquid biopsies,FFPE, exosomes, serum, or plasma.

  • Description
    • Project size: minimum of 24 samples
    • Sample type: total isolated RNA
    • Required quantities: minimum 5 ng of total RNA (Human, Mouse, Rat) or 100 ng (Plant)
    • Shipment conditions: shipped in 1.5 mL or 0.5 mL tubes on dry ice
  • Services Workflow
    Total RNA-seq Service

    What our service includes

    1. RNA quality control
      • Determination of RIN value (RNA integration number)
      • Measurement of RNA concentration
    2. Library preparation
      • Optional rRNA depletion
      • Stranded library construction
      • Quality control of libraries prior to sequencing
    3. Sequencing on Illumina platforms
      • 50 million raw reads per sample
    4. Data analysis (optional for additional fee)
      • Standard bioinformatic analysis
      • Advanced bioinformatic analysis
      • Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request
  • Bioinformatic analysis

    Standard bioinformatic analysis
    (optional for additional fee)

    Advanced bioinformatic analysis
    (optional for additional fee)

    • Demultiplexing, read quality control, trimming and filtering
    • Alignment to reference genome
    • Quantification of coding and non-coding RNA biotypes with specialized quantification tool MGCount (software developed by Diagenode)

    Provided files:

    • Report with sequencing statistics
    • Raw data in FASTQ format
    • FastQC report
    • Alignment in BAM format
    • Un-normalized read counts
    • Differential expression analysis
    • Gene ontology terms analysis
    • Pathway analysis
    • Alternative splicing analysis
    • Novel transcript identification

  •  文档
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  •  出版物

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