RNA-seq services

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			'description' => '<p><span style="font-weight: 400;">RNA sequencing uses next-generation sequencing (NGS) to uncover the presence and quantity of RNA for gene expression profiling across the transcriptome. Diagenode specializes in generating RNA-seq libraries which is optimal for low or degraded inputs including those from liquid biopsies,FFPE, exosomes, serum, or plasma.</span></p>
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<li><strong>Project size</strong>: minimum of 24 samples</li>
<li><strong>Sample type</strong>: total isolated RNA</li>
<li><strong>Required quantities</strong>: minimum 5 ng of total RNA (Human, Mouse, Rat) or 100 ng (Plant)</li>
<li><strong>Shipment conditions</strong>: shipped in 1.5 mL or 0.5 mL tubes on dry ice</li>
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<h3 class="green">What our service includes</h3>
<ol>
<li><strong>RNA quality control</strong></li>
<ul>
<li>Determination of RIN value (RNA integration number)</li>
<li>Measurement of RNA concentration</li>
</ul>
<li><strong>Library preparation</strong></li>
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<li>Optional rRNA depletion</li>
<li>Stranded library construction</li>
<li>Quality control of libraries prior to sequencing</li>
</ul>
<li><strong>Sequencing on Illumina platforms</strong></li>
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<li>50 million raw reads per sample</li>
</ul>
<li><strong>Data analysis</strong> (optional for additional fee)</li>
<ul>
<li>Standard bioinformatic analysis</li>
<li>Advanced bioinformatic analysis</li>
<li>Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request</li>
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<h4 class="text-center"><strong>Standard bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
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<h4 class="text-center"><strong>Advanced bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
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<li>Demultiplexing, read quality control, trimming and filtering</li>
<li>Alignment to reference genome</li>
<li>Quantification of coding and non-coding RNA biotypes with specialized quantification tool MGCount (<a href="https://doi.org/10.1186/s12859-021-04544-3">software developed by Diagenode</a>)</li>
</ul>
<br />
<p><em>Provided files:</em></p>
<ul>
<li>Report with sequencing statistics</li>
<li>Raw data in FASTQ format</li>
<li>FastQC report</li>
<li>Alignment in BAM format</li>
<li>Un-normalized read counts</li>
</ul>
</td>
<td style="vertical-align: top;">
<ul>
<li>Differential expression analysis</li>
<li>Gene ontology terms analysis</li>
<li>Pathway analysis</li>
<li>Alternative splicing analysis</li>
<li>Novel transcript identification</li>
</ul>
</td>
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<li><strong>Required quantities</strong>: minimum 5 ng of total RNA (Human, Mouse, Rat) or 100 ng (Plant)</li>
<li><strong>Shipment conditions</strong>: shipped in 1.5 mL or 0.5 mL tubes on dry ice</li>
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<h3 class="green">What our service includes</h3>
<ol>
<li><strong>RNA quality control</strong></li>
<ul>
<li>Determination of RIN value (RNA integration number)</li>
<li>Measurement of RNA concentration</li>
</ul>
<li><strong>Library preparation</strong></li>
<ul>
<li>Optional rRNA depletion</li>
<li>Stranded library construction</li>
<li>Quality control of libraries prior to sequencing</li>
</ul>
<li><strong>Sequencing on Illumina platforms</strong></li>
<ul>
<li>50 million raw reads per sample</li>
</ul>
<li><strong>Data analysis</strong> (optional for additional fee)</li>
<ul>
<li>Standard bioinformatic analysis</li>
<li>Advanced bioinformatic analysis</li>
<li>Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request</li>
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</td>
<td>
<h4 class="text-center"><strong>Advanced bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
</tr>
<tr>
<td style="vertical-align: top; padding-left: 20px;">
<ul>
<li>Demultiplexing, read quality control, trimming and filtering</li>
<li>Alignment to reference genome</li>
<li>Quantification of coding and non-coding RNA biotypes with specialized quantification tool MGCount (<a href="https://doi.org/10.1186/s12859-021-04544-3">software developed by Diagenode</a>)</li>
</ul>
<br />
<p><em>Provided files:</em></p>
<ul>
<li>Report with sequencing statistics</li>
<li>Raw data in FASTQ format</li>
<li>FastQC report</li>
<li>Alignment in BAM format</li>
<li>Un-normalized read counts</li>
</ul>
</td>
<td style="vertical-align: top;">
<ul>
<li>Differential expression analysis</li>
<li>Gene ontology terms analysis</li>
<li>Pathway analysis</li>
<li>Alternative splicing analysis</li>
<li>Novel transcript identification</li>
</ul>
</td>
</tr>
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			'description' => '<h2 class="text-center"><span class="diacol">New!</span> <br />Data mining using machine learning (AI) for unique epigenetic data insights</h2>
<center><img src="https://www.diagenode.com/img/product/data-mining-long.png" /></center>
<p></p>
<div id="paper" style="text-align: center;">
<h4><a href="https://www.youtube.com/watch?v=KXjnSHz3Jk8">Watch the webinar to gain insights on how data mining can be applied for epigenetics applications</a></h4>
</div>
<center><iframe width="560" height="315" src="https://www.youtube.com/embed/KXjnSHz3Jk8" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen="allowfullscreen"></iframe></center>
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<div class="content active" id="panel1">
<h3 style="margin-top: 0;">Powerful new insights with epigenetic data mining.<br /> A study to distinguish smokers from non-smokers using just one droplet of blood</h3>
<p>Next generation sequencing in combination with sophisticated bioinformatics technologies for genomic, transcriptomic and epigeneomic analyses have enormous potential to establish new biomarkers for disease diagnostics, enabling true precision medicine. Analyses of liquid biopsies to measure thousands of different data points simultaneously in easily accessible body fluids (e.g. blood, urine, and saliva) are extremely promising for such biomarker studies.<br />(...)</p>
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<h3 style="margin-top: 0;">Data mining on DNA methylation data in cancer samples<br />Distinguishing normal from breast cancer tissue</h3>
<p>Breast cancer is the most commonly occurring cancer in women and the second most common cancer overall.</p>
<p>One important aspect of cancer tissues it that they differ from normal tissues in their epigenetic make up, especially in the DNA methylation pattern. In normal cells methylation assures the proper regulation of gene expression and stable gene silencing. DNA methylation is associated with histone modifications, and the interplay of these epigenetic modifications is crucial to regulate the functioning of the genome by changing chromatin architecture.<br />(...)</p>
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<p>Diagenode's new data mining service utilizes methods at the frontier of machine learning, statistics, and database systems. This enhanced service supports new discoveries that were previously not possible by analyzing patterns in large data sets to give informative new insights.</p>
<p>If you have data from patient cohorts, single cell analyses or any other heterogeneous scenarios, our service team provides enhanced support with optimal data analysis using our latest data mining capabilities. Specifically, our team applies machine learning technologies to find previously undiscovered or unobvious relationships within and across datasets. This advanced technology allows discovery of informative features from mass data, essentially &ldquo;finding a needle in a haystack.&rdquo;</p>
<p>Diagenode utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data. Our goal is to build strong classifiers that separate data into two or more classes or groups depending on associated data.</p>
<p class="extra-spaced">Different and multiple -omics data classes can be mined simultaneously. Integration with phenotypic and/or clinical data is also possible. We offer data mining services for several data classes including:</p>
<table class="extra-spaced">
<tbody>
<tr>
<td><strong>Epigenetic data</strong></td>
<td><strong>Transcriptomic data</strong></td>
</tr>
<tr>
<td>
<p>DNA Methylation (RRBS, WGBS, EPIC arrays)</p>
<p>ChIP-sequencing</p>
<p>ATAC-seq</p>
</td>
<td>
<p>mRNA-sequencing</p>
<p>Small and long non coding RNA</p>
<p>Single-cell RNA-sequencing</p>
</td>
</tr>
</tbody>
</table>
<p></p>
<p><strong>Biological Interpretation</strong></p>
<p class="extra-spaced">Machine learning classifiers also mirror the underlying biological differences between classes and are used to uncover the molecular processes at work. In order to achieve this, we offer biological interpretation services and pathway mining analyses for your data.</p>
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<li><strong>Feasibility study</strong></li>
</ol>
<ul>
<li>Assessment of data characteristics and <span></span>applicability of different machine learning (ML)</li>
<li>Prototypic analyses:</li>
<li>Initial feasibility report</li>
</ul>
<p></p>
<ol start="2">
<li><strong>Data Mining</strong></li>
</ol>
<ul>
<li>Machine learning on data</li>
<li>Data evaluation and validation</li>
<li>Report generation</li>
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<p>&nbsp;</p>
<ol start="3">
<li><strong>Data Interpretation</strong></li>
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<p><a href="https://www.diagenode.com/en/categories/Services">Read about our wetlab services</a></p>
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			'name' => 'mRNA-seq Service',
			'description' => '<p><strong>mRNA-seq</strong> is the method of choice to efficiently detect and precisely quantify coding RNAs present in a biological sample under specific circumstances. Accurate estimation of transcript abundance allows for differential expression analysis as well as the study of alternative splicing and discovery of new transcript isoforms.</p>
<p>Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of mRNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on specific poly A selection to produce Illumina compatible libraries enriched in mRNAs.</p>
<h2>High performance mRNA-seq</h2>
<ul class="square">
<li>Superior complexity and high transcript coverage</li>
<li>Accurate comparison of gene expression levels</li>
<li>Detection of novel transcripts and alternative splicing events</li>
<li>Suitable for a broad range of input amounts</li>
<li>Compatible with FFPE samples</li>
<li>Fast turnaround time (from 5 to 6 weeks)</li>
</ul>
<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/rna-seq-category">See our other RNA-seq Profiling Services</a></p>',
			'label1' => 'Sample Requirements',
			'info1' => '<div class="extra-spaced">
<ul>
<li><strong>Project size</strong>: minimum of 24 samples</li>
<li><strong>Sample type</strong>: total isolated RNA</li>
<li><strong>Required quantities</strong>: minimum 10 ng of total RNA</li>
<li><strong>Shipment conditions</strong>: shipped in 1.5 mL or 0.5 mL tubes on dry ice</li>
</ul>
</div>',
			'label2' => 'Services Workflow',
			'info2' => '<center><img alt="mRNA-seq Service" src="https://www.diagenode.com/img/product/services/mRNA-seq_workflow.png" /></center>
<div class="extra-spaced">
<h3 class="green">What our service includes</h3>
<ol>
<li><strong>RNA quality control</strong></li>
<ul>
<li>Determination of RIN value (RNA integration number)</li>
<li>Measurement of RNA concentration</li>
</ul>
<li><strong>Library preparation</strong></li>
<ul>
<li>Poly-A selection</li>
<li>Stranded library construction</li>
<li>Quality control of libraries prior to sequencing</li>
</ul>
<li><strong>Sequencing on Illumina platforms</strong></li>
<ul>
<li>25-30 million raw reads per sample</li>
</ul>
<li><strong>Data analysis</strong> (optional for additional fee)</li>
<ul>
<li>Standard bioinformatic analysis</li>
<li>Advanced bioinformatic analysis</li>
<li>Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request</li>
</ul>
</ol>
</div>',
			'label3' => 'Bioinformatics Analysis',
			'info3' => '<table style="padding: 40px;">
<tbody>
<tr>
<td>
<h4 class="text-center"><strong>Standard bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
<td>
<h4 class="text-center"><strong>Advanced bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
</tr>
<tr>
<td style="vertical-align: top; padding-left: 20px;">
<ul>
<li>Demultiplexing, read quality control, trimming and filtering</li>
<li>Alignment to reference genome</li>
<li>Quantification of known transcripts</li>
</ul>
<br />
<p><em>Provided files:</em></p>
<ul>
<li>Report with sequencing statistics</li>
<li>Raw data in FASTQ format</li>
<li>FastQC report</li>
<li>Alignment in BAM format</li>
<li>Un-normalized read counts</li>
</ul>
</td>
<td style="vertical-align: top;">
<ul>
<li>Differential expression analysis</li>
<li>Gene ontology terms analysis</li>
<li>Pathway analysis</li>
<li>Alternative splicing analysis</li>
<li>Novel transcript identification</li>
</ul>
</td>
</tr>
</tbody>
</table>
<p></p>',
			'format' => 'Custom',
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			'slug' => 'mrna-seq-services',
			'meta_title' => 'mRNA-seq Service - Coding Transcriptome Analysis| Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'mRNA-seq provides a complete view of the coding transcriptome. mRNA-Seq detects known and novel transcripts and measures transcript abundance for accurate, comprehensive analysis.',
			'modified' => '2020-12-24 12:47:26',
			'created' => '2020-07-13 10:52:47',
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		(int) 2 => array(
			'id' => '3089',
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			'name' => 'Small RNA-seq Service',
			'description' => '<p><strong>Small RNA-seq</strong> is the method of choice to investigate the regulatory network of small non-coding RNAs (&lt;200 nt length), including but not limited to miRNA transcripts. It utilizes the proprietary technology D-Plex which ensures realistic representation of diverse small non-coding RNA transcripts with minimized bias. This approach is optimal for finding biomarkers from known transcripts and discover new RNA features.</p>
<p>Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of small RNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on a template-switching technology to produce Illumina compatible libraries enriched with the large variety of small non-coding RNAs.</p>
<h2>High transcript diversity with small RNA-seq</h2>
<ul class="square">
<li>Realistic representation of all small non-coding RNAs</li>
<li>Detection of novel transcripts and differentially-expressed small RNAs</li>
<li>Investigation of post-transcriptional regulation of gene expression</li>
<li>Leveraging the <a href="https://www.diagenode.com/en/pages/dplex">D-Plex technology</a></li>
<li>Working with ultra-low RNA inputs</li>
<li>Compatible with biological fluids</li>
</ul>
<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/rna-seq-category">See our other RNA-seq Profiling Services</a></p>',
			'label1' => 'Sample Requirements',
			'info1' => '<div class="extra-spaced">
<ul>
<li><strong>Project size</strong>: no minimum</li>
<li><strong>Sample type</strong>: total isolated RNA or small enriched RNA</li>
<li><strong>Required quantities</strong>: minimum 100 pg of total RNA or 10 pg of small RNA</li>
<li><strong>Shipment conditions</strong>: shipped in 1.5 mL or 0.5 mL tubes on dry ice</li>
</ul>
</div>',
			'label2' => 'Services Workflow',
			'info2' => '<center><img alt="Small RNA-seq Service" src="https://www.diagenode.com/img/product/services/sncRNA-seq_workflow.png" /></center>
<div class="extra-spaced">
<h3 class="green">What our service includes</h3>
<ol>
<li><strong>RNA quality control</strong></li>
<ul>
<li>Determination of RIN value (RNA integration number)</li>
<li>Measurement of RNA concentration</li>
</ul>
<li><strong>Library preparation</strong></li>
<ul>
<li>Capture and amplification by tailing and switching</li>
<li>Library construction</li>
<li>Quality control of libraries prior to sequencing</li>
</ul>
<li><strong>Sequencing on Illumina platforms</strong></li>
<ul>
<li>10-20 million raw reads per sample</li>
</ul>
<li><strong>Data analysis</strong> (optional for additional fee)</li>
<ul>
<li>Standard bioinformatic analysis</li>
<li>Advanced bioinformatic analysis</li>
<li>Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request</li>
</ul>
</ol>
</div>',
			'label3' => 'Bioinformatics Analysis',
			'info3' => '<table style="padding: 40px;">
<tbody>
<tr>
<td>
<h4 class="text-center"><strong>Standard bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
<td>
<h4 class="text-center"><strong>Advanced bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
</tr>
<tr>
<td style="vertical-align: top; padding-left: 20px;">
<ul>
<li>Demultiplexing, read quality control, trimming and filtering</li>
<li>Alignment to reference genome</li>
<li>Quantification of small RNA biotypes with specialized quantification tool MGCount (<a href="https://doi.org/10.1186/s12859-021-04544-3">software developed by Diagenode</a>)</li>
</ul>
<br />
<p><em>Provided files:</em></p>
<ul>
<li>Report with sequencing statistics</li>
<li>Raw data in FASTQ format</li>
<li>FastQC report</li>
<li>Alignment in BAM format</li>
<li>Un-normalized read counts</li>
</ul>
</td>
<td style="vertical-align: top;">
<ul>
<li>Differential expression analysis</li>
<li>Gene ontology terms analysis</li>
<li>Pathway analysis</li>
</ul>
</td>
</tr>
</tbody>
</table>
<p></p>',
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			'old_catalog_number' => '',
			'sf_code' => '',
			'type' => 'ACC',
			'search_order' => '01-Accessory',
			'price_EUR' => 'on request',
			'price_USD' => 'on request',
			'price_GBP' => 'on request',
			'price_JPY' => '42800',
			'price_CNY' => 'on request',
			'price_AUD' => 'on request',
			'country' => 'ALL',
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			'master' => true,
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			'slug' => 'small-rna-seq-services',
			'meta_title' => 'Small RNA-seq Service - Small RNA and miRNA profiling | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'Small RNA-seq enables small RNA discovery, mutation characterization, and expression profiling of small RNAs by leveraging of sequencing technologies and data analysis pipeline.',
			'modified' => '2022-03-07 09:12:41',
			'created' => '2020-07-13 11:09:54',
			'ProductsRelated' => array(
				[maximum depth reached]
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		),
		(int) 3 => array(
			'id' => '3062',
			'antibody_id' => null,
			'name' => 'RNA Data Analysis',
			'description' => '<p>Total RNA sequencing (RNA-Seq) detects both coding and noncoding RNAs and is typically used to measure gene and transcript abundance as well as to identify novel components of the transcriptome. Messenger RNA-Seq focuses on the quantification of gene expression, the identification of unknown transcripts, the discovery of alternative splicing and gene fusion events. And finally, small non-coding RNA sequencing (sncRNA-Seq) will detect small (&lt;100 nucleotides long) RNAs that operate as key regulators in cellular processes.</p>
<div class="extra-spaced">
<h2>What do we provide with the analysis?</h2>
<p>This analysis provides information for either genes or isoforms with their expression levels.</p>
<h3 class="diacol" style="font-weight: 100;">Standard Analysis</h3>
<ul style="list-style-type: circle;">
<li>Summary statistics (total sequenced reads, total mapping reads, uniquely aligned reads, PCR duplicates, number of genes detected, average read density per gene, number of highly expressed genes, etc.)</li>
<li>Trimmed and filtered reads in fastQ files after sequencing QC</li>
<li>BAM sorted files from alignment to reference genome or transcriptome (indexed bam files and bigwig files included)</li>
<li>Matrix with expression abundance obtained with specialized quantification tool MGCount (<a href="https://doi.org/10.1186/s12859-021-04544-3">software developed by Diagenode</a>). A table of MG communities linking each original feature in the GTF file with the resultant count matrix and metadata feature identifiers.</li>
</ul>
<h3 class="diacol" style="font-weight: 100;">Advanced Analysis</h3>
<ul style="list-style-type: circle;">
<li>Comparative analysis (also called differential analysis) aimed at finding differentially expressed genes (DEGs) between two groups of samples</li>
<li>Functional gene annotation</li>
<li>Gene ontology enrichment analysis on DEGs</li>
<li>Pathway enrichment analysis on DEGs (KEGG or DOSE for human samples)</li>
<li>Alternative splicing analysis</li>
<li>Gene fusion analysis</li>
<li>Novel transcript identification</li>
</ul>
<h3 class="diacol" style="font-weight: 100;">Customized Analysis</h3>
<p class="text-left">If you require a type of analysis that is not in the previous list, <a href="#" data-reveal-id="quoteModal-3062">please consult with our expert bioinformatics team</a>.</p>
</div>
<div><center><img src="https://www.diagenode.com/img/product/services/RNA-theorie.png" /></center></div>
<p><a href="https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-021-04544-3">MGcount</a>: Diagenode has developed a bioinformatics software for counting whole-transcriptome RNA-seq reads from one or more input alignment files. It is specially designed to incorporate multi-mapping and multi-overlapping reads in the quantification using a flexible methodology that is compatible with any biotype. At the end of its execution, it produces a count matrix, compatible with any downstream analysis.</p>
<center><img src="https://www.diagenode.com/img/product/services/mGcount-img.png" width="600px" /></center>',
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			'price_USD' => '/',
			'price_GBP' => '/',
			'price_JPY' => '42800',
			'price_CNY' => '/',
			'price_AUD' => '/',
			'country' => 'ALL',
			'except_countries' => 'None',
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			'online' => true,
			'master' => true,
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			'slug' => 'rna-data-analysis',
			'meta_title' => 'RNA Data Analysis Services | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'RNA-seq data analysis service is made to extract information from a large variety of  RNA-seq data (small RNA, mRNA and much more).',
			'modified' => '2022-11-22 15:26:24',
			'created' => '2020-03-26 10:04:58',
			'ProductsRelated' => array(
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			'Image' => array([maximum depth reached])
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	'Application' => array(
		(int) 0 => array(
			'id' => '51',
			'position' => '0',
			'parent_id' => '46',
			'name' => '小型RNAとmiRNA',
			'description' => '<ul>
<li><span> </span>遺伝子発現の転写後調節</li>
<li><span> </span>新規転写物および差示的に発現される小RNA</li>
<li><span> </span>腫瘍抑制遺伝子発現の調節に関与するmiRNA</li>
<li><span> </span>差次的miRNA発現</li>
</ul>',
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			'slug' => 'small-rna-and-mirna',
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			'modified' => '2018-01-12 15:03:17',
			'created' => '2017-11-20 16:22:17',
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				[maximum depth reached]
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		(int) 1 => array(
			'id' => '52',
			'position' => '0',
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			'name' => 'メッセンジャーRNA(ｍRNA)',
			'description' => '<ul>
<li><span> </span>特異的ポリA選定で<span>タンパク質コーディング</span><span>RNA</span><span></span><span></span></li>
<li><span> </span>がん関連mRNAシグネチャー</li>
<li><span> </span>癌の病因における異常なmRNA転換</li>
<li><span> </span>遺伝子発現プロファイリングおよび示差的に発現される遺伝子の同定（DEG）</li>
<li><span> </span>遺伝子発現のエピジェネティックな機構（トランスクリプトーム+ H3K36me3およびH3K4me3標識）</li>
<li><span> </span>コーディング遺伝子の機能的アノテーション</li>
</ul>',
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		(int) 2 => array(
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			'parent_id' => '46',
			'name' => '全トランスクリプトーム解析と長い非コードRNA（lncRNA）解析',
			'description' => '<ul>
<li><span> </span>トランスクリプトーム配列決定：任意のrRNA<span>除去</span>を伴う200ntより大きい<span>RNA</span><span>および</span>全トランスクリプトーム<span></span><span></span>の分析</li>
<li><span> </span>クロマチンリモデリングおよび転写/転写後調節に関与する非タンパク質コードRNA</li>
</ul>
<p><span>&nbsp;</span></p>',
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			'modified' => '2018-01-12 16:29:08',
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	'Category' => array(
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			'id' => '121',
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			'name' => 'Epigenomics Profiling Services',
			'description' => '<div class="row">
<div class="small-12 medium-10 large-10 columns text-left">
<p>Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis.&nbsp;</p>
</div>
<div class="small-12 medium-2 large-2 columns text-left"><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></div>
</div>
<center><img alt="DNA-RNA-Chromatin-Diagenode" src="https://www.diagenode.com/img/categories/services/chromatin-dna-rna.png" /></center>
<div class="row">
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #099f92;">
<h3><span class="green">Chromatin</span>&nbsp;analysis</h3>
<ul>
<li>ChIP-seq analysis Histone modification</li>
<li>Promoter analysis</li>
<li>Enhancer analysis</li>
<li>ChIP-seq analysis Transcription factor&nbsp;</li>
<li>(sc)ATAC-seq Chromatin accessibility</li>
<li>ChIP-seq Cas9 off-target</li>
<li>Customized NGS service</li>
</ul>
<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/chip-seq-service">Learn more</a></p>
</div>
</div>
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #474546;">
<h3><span class="darkgrey">DNA methylation</span> analysis</h3>
<ul>
<li>Reduced representation bisulfite sequencing (RRBS)</li>
<li>Whole genome bisulfite sequencing (WGBS)</li>
<li>Targeted methylation</li>
<li>MeDIP-seq/hMeDIP-seq</li>
<li>Infinium MethylationEPIC array&nbsp;</li>
</ul>
<p></p>
<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/dna-methylation-profiling-services">Learn more</a></p>
</div>
</div>
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #b11e33;">
<h3><span class="diacol"><g class="gr_ gr_44 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="44" data-gr-id="44">RNA-seq</g></span> analysis</h3>
<ul>
<li><span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;">Gene expression profiling</span></li>
<li>mRNA<span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;"> analysis</span></li>
<li>Small non-coding RNA analysis</li>
<li><b></b>Whole transcriptome analysis<br /><br /><br /><br /><br /></li>
</ul>
<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/rna-seq-category">Learn more</a></p>
</div>
</div>
</div>
<h2 style="text-align: center;"><a class="details radius small button" href="https://www.diagenode.com/en/documents/services-flyer">DOWNLOAD OUR FLYER</a></h2>
<h2>Why Diagenode</h2>
<ul>
<li><strong>Expertise and trust</strong>: Recognized epigenetics leader, official partner of <a href="http://www.blueprint-epigenome.eu/"><g class="gr_ gr_45 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="45" data-gr-id="45">BLUEPRINT</g></a>, <a href="http://ihec-epigenomes.org/">IHEC</a> <g class="gr_ gr_46 gr-alert gr_gramm gr_inline_cards gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="46" data-gr-id="46">and</g> <a href="https://www.faang.org/">FAANG</a></li>
<li><strong>Innovative technology</strong>: Utilization of the signature Bioruptor<sup>&reg;</sup> sonication device for optimal chromatin and DNA shearing and the IP-Star<sup>&reg;</sup> Automation device give reproducible and reliable optimization and results</li>
<li><strong>Quality</strong>: Multiple QC steps in all workflows and validated antibodies plus reagents deliver superior data</li>
<li><strong>Flexibility</strong>: Extensive range of sample species and sample origins</li>
<li><strong>Experience</strong> in epigenomics profiling for a wide range of applications and fields of interest</li>
</ul>
<div class="extra-spaced">
<ul class="small-block-grid-2 medium-block-grid-3 large-block-grid-3">
<li>
<div class="block-distri"><span class="label-green" style="margin-bottom: 16px; margin-left: -3px;">HUMAN</span><center><img width="260" height="259" alt="services chip-seq - human" src="https://www.diagenode.com/img/categories/services/services-humans.png" caption="false" /></center></div>
</li>
<li>
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<h2>12 years of expertise and trust in epigenetics</h2>
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<li><strong>End-to-end</strong> epigenetic service</li>
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<li>Dedicated <strong>in-house expert</strong> for your project</li>
<li><strong>Integrative</strong> data analysis</li>
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<div class="panel"><center><img alt="RNA-seq-Diagenode" src="http://www.diagenode.com/img/categories/services/rna-workflow.png" /></center>
<p><br />RNA-seq is a revolutionary technique that examines the total cellular content of RNAs, the transcriptome, using deep-sequencing technologies. Obtaining data about the transcriptome allows to connect the information on a genome with its functional protein expression. It provides great insights into cell development mechanisms and disease-associated modifications.</p>
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<center><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></center></div>
<div class="small-12 medium-8 large-8 columns"><a href="https://www.diagenode.com/en/p/high-throughput-3mrna-seq-services"></a>
<h3><a href="https://www.diagenode.com/en/p/mrna-seq-services">mRNA analysis</a></h3>
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Our Epigenomics Profiling Services helps you to complete your transcriptomic profiling analysis using next-generation sequencing (NGS). Our experienced scientists will help you design your RNA-seq experiments based on the ultimate goal of your transcriptomic profiling analysis by generating meaningful RNA-seq libraries and delivering high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</div>
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<p>Epigenetics is crucial for the regulation of gene expression and has broad relevance in biological processes like development, disease and response to the environment. For more than 10 years Diagenode has been developing innovative tools to study epigenetic marks such as post-translational modi cations of histones and DNA methylation. We are now utilizing our expertise by offering custom services. Our <a href="https://www.diagenode.com/en/categories/Services">services</a> include full work ows for ChIP-sequencing as well as reduced representation bisul te sequencing (RRBS) with our new optimized &ldquo;Premium RRBSTM technology. In addition, we also offer bioinformatic analysis of your results, both standard and customized. The <a href="https://www.diagenode.com/en/categories/Services">Diagenode Epigenetics Custom Services</a> helps you to complete your epigenetics work ow from your starting biological material to your nal results.</p>
<p><a href="https://www.diagenode.com/en/categories/Services">Learn more</a></p>',
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<li>Chromatin analysis</li>
<li>DNA methylation services</li>
<li>RNA-seq analysis</li>
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			'description' => '<h6>Background and purpose</h6>
<div class="section-paragraph">Traumatic brain injury (TBI) and its consequences remain great challenges for neurology. Consequences of TBI are associated with various alterations in the brain but little is known about long-term changes of epigenetic DNA methylation patterns. Moreover, nothing is known about potential treatments that can alter these epigenetic changes in beneficial ways. Therefore, we have examined myo-inositol (MI), which has positive effects on several pathological conditions.</div>
<h6></h6>
<h6>Methods</h6>
<div class="section-paragraph">TBI was induced in mice by controlled cortical impact (CCI). One group of CCI animals received saline injections for two months (TBI+SAL), another CCI group received MI treatment (TBI+MI) for the same period and one group served as a sham-operated control. Mice were sacrificed 4 months after CCI and changes in DNA methylome and transcriptomes were examined.</div>
<h6></h6>
<h6>Results</h6>
<div class="section-paragraph">For the first time we: (i) provide comprehensive map of long-term DNA methylation changes after CCI in the hippocampus; (ii) identify differences by methylation sites between the groups; (iii) characterize transcriptome changes; (iv) provide association between DNA methylation sites and gene expression. MI treatment is linked with upregulation of genes covering 33 biological processes, involved in immune response and inflammation. In support of these findings, we have shown that expression of BATF2, a transcription factor involved in immune-regulatory networks, is upregulated in the hippocampus of the TBI+MI group where the BATF2 gene is demethylated.</div>
<h6></h6>
<h6>Conclusion</h6>
<div class="section-paragraph">TBI is followed by long-term epigenetic and transcriptomic changes in hippocampus. MI treatment has a significant effect on these processes by modulation of immune response and biological pathways of inflammation.</div>',
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            </div>

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        <div class="small-12 columns" >
            <h6 style="height:60px">Bioinformatics Data Mining Service</h6>
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                         <a href="/cn/p/mrna-seq-services"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



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        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>mRNA-seq Service</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

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            <h6 style="height:60px">mRNA-seq Service</h6>
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            <span class="prefix">Total number of samples (including replicates)</span>
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        <div class="small-12 medium-12 large-6 columns">
            <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/>        </div>
    </div>

<div class="row collapse">
    <h2>Contact Information</h2>
    <div class="small-3 large-2 columns">
        <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Phone number</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">City</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-3089">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d&#039;Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People&#039;s Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People&#039;s Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
<option value="SG">Singapore</option>
<option value="SX">Sint Maarten (Dutch part)</option>
<option value="SK">Slovakia</option>
<option value="SI">Slovenia</option>
<option value="SB">Solomon Islands</option>
<option value="SO">Somalia</option>
<option value="ZA">South Africa</option>
<option value="GS">South Georgia and the South Sandwich Islands</option>
<option value="ES">Spain</option>
<option value="LK">Sri Lanka</option>
<option value="SD">Sudan</option>
<option value="SR">Suriname</option>
<option value="SS">South Sudan</option>
<option value="SJ">Svalbard and Jan Mayen</option>
<option value="SZ">Swaziland</option>
<option value="SE">Sweden</option>
<option value="CH">Switzerland</option>
<option value="SY">Syrian Arab Republic</option>
<option value="TW">Taiwan</option>
<option value="TJ">Tajikistan</option>
<option value="TZ">Tanzania</option>
<option value="TH">Thailand</option>
<option value="TL">Timor-Leste</option>
<option value="TG">Togo</option>
<option value="TK">Tokelau</option>
<option value="TO">Tonga</option>
<option value="TT">Trinidad and Tobago</option>
<option value="TN">Tunisia</option>
<option value="TR">Turkey</option>
<option value="TM">Turkmenistan</option>
<option value="TC">Turks and Caicos Islands</option>
<option value="TV">Tuvalu</option>
<option value="UG">Uganda</option>
<option value="UA">Ukraine</option>
<option value="AE">United Arab Emirates</option>
<option value="GB">United Kingdom</option>
<option value="US" selected="selected">United States</option>
<option value="UM">United States Minor Outlying Islands</option>
<option value="UY">Uruguay</option>
<option value="UZ">Uzbekistan</option>
<option value="VU">Vanuatu</option>
<option value="VE">Venezuela</option>
<option value="VN">Viet Nam</option>
<option value="VG">Virgin Islands, British</option>
<option value="VI">Virgin Islands, U.S.</option>
<option value="WF">Wallis and Futuna</option>
<option value="EH">Western Sahara</option>
<option value="YE">Yemen</option>
<option value="ZM">Zambia</option>
<option value="ZW">Zimbabwe</option>
</select><script>
    $('#country_selector_quote-3089').selectize();
</script><br />
    </div>
</div>
<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">State</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][state]" id="state-3089" maxlength="3" type="text"/><br />
    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/>    </div>
</div>
<div class="row collapse" id="email_v">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/>    </div>
</div>


<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Comment</span>
    </div>
    <div class="small-9 large-10 columns">
        <textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea>    </div>
</div>

<!------------SERVICES PARTICULAR FORM START---------------->


<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->


<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <div class="recaptcha"><div id="recaptcha662a53be58d63"></div></div>    </div>
</div>
<br />

<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <button id="submit_btn-3089" class="alert button expand" form="Quote-3089" type="submit">Contact me</button>    </div>
</div>
</form><script>
    var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c';

    function postToPardot(formAction, id) {
        $('#pardot-form-handler').load(function(){
            $('#Quote-' + id).attr('action', formAction);
            $('#Quote-' + id).submit();
        });
        $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize());
        
    }
    $(document).ready(function() {
        $('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>');
		$('#Quote-3089').attr('action','javascript:postToPardot(\'' + $('#Quote-3089').attr('action') + '\', 3089)');
    });

    $("#Quote-3089 #submit_btn-3089").click(function (e) {
            
        if($(this).closest('form')[0].checkValidity()){
            e.preventDefault();
            
            //disable the submit button
            $("#Quote-3089 #submit_btn-3089").attr("disabled", true);
            $("#Quote-3089 #submit_btn-3089").html("Processing...");

            //submit the form
            $("#Quote-3089").submit();
        } 
    })
</script>
<script>
    if ($("#Quote-3089 #country_selector_quote-3089.selectized").val() == 'US') {
        var val = $("#state-3089").val();
        $("#Quote-3089 #state-3089").replaceWith('<select name="data[Quote][state]" id="state-3089" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
        if (val.length == 2) {
            $("#state-3089").val(val);
        }
        $("#state-3089").parent().parent().show();
    } else if ($("#Quote-3089 #country_selector_quote-3089.selectized").val() == 'CA') {
        var val = $("#state-3089").val();
        $("#Quote-3089 #state-3089").replaceWith('<select name="data[Quote][state]" id="state-3089" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
        if (val.length == 2) {
            $("#state-3089").val(val);
        }
        $("#state-3089").parent().parent().show();
    } else if ($("#Quote-3089 #country_selector_quote-3089.selectized").val() == 'DE') {
        var val = $("#state-3089").val();
        $("#Quote-3089 #state-3089").replaceWith('<select name="data[Quote][state]" id="state-3089" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
        if (val.length == 2) {
            $("#state-3089").val(val);
        }
        $("#state-3089").parent().parent().show();
    } else {
        $("#Quote-3089 #state-3089").parent().parent().hide();
        $("#Quote-3089 #state-3089").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3089" value="">');
    }

    $("#Quote-3089 #country_selector_quote-3089.selectized").change(function() {
        if (this.value == 'US') {
            $("#Quote-3089 #state-3089").replaceWith('<select name="data[Quote][state]" id="state-3089" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
            $("#Quote-3089 #state-3089").parent().parent().show();
        } else if (this.value == 'CA') {
            $("#Quote-3089 #state-3089").replaceWith('<select name="data[Quote][state]" id="state-3089" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
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<a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: QUOTE MODAL --><a href="#" id="small-rna-seq-services" data-reveal-id="quoteModal-3089" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Small RNA-seq Services</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/cn/p/rna-data-analysis"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
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<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>RNA Data Analysis</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

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<div class="row collapse">
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<div class="row collapse">
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<!------------SERVICES PARTICULAR FORM START---------------->


<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->


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            <h6 style="height:60px">RNA Data Analysis</h6>
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<p><a href="https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-021-04544-3">MGcount</a>: Diagenode has developed a bioinformatics software for counting whole-transcriptome RNA-seq reads from one or more input alignment files. It is specially designed to incorporate multi-mapping and multi-overlapping reads in the quantification using a flexible methodology that is compatible with any biotype. At the end of its execution, it produces a count matrix, compatible with any downstream analysis.</p>
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			'description' => '<h2 class="text-center"><span class="diacol">New!</span> <br />Data mining using machine learning (AI) for unique epigenetic data insights</h2>
<center><img src="https://www.diagenode.com/img/product/data-mining-long.png" /></center>
<p></p>
<div id="paper" style="text-align: center;">
<h4><a href="https://www.youtube.com/watch?v=KXjnSHz3Jk8">Watch the webinar to gain insights on how data mining can be applied for epigenetics applications</a></h4>
</div>
<center><iframe width="560" height="315" src="https://www.youtube.com/embed/KXjnSHz3Jk8" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen="allowfullscreen"></iframe></center>
<p></p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<blockquote style="padding-bottom: 0;"><span class="label-green" style="margin-bottom: 16px; margin-left: -22px; font-size: 22px;">WHITE PAPERS</span>
<div id="portal" class="main-portal">
<div class="portal-inner"><nav class="portal-nav" style="text-align: left;">
<ul data-tab="" class="tips-menu">
<li><a href="#panel1" class="tips portal button">Smokers vs non-smokers </a></li>
<li><a href="#panel2" class="tips portal button">Breast cancer</a></li>
</ul>
</nav></div>
</div>
<div class="tabs-content">
<div class="content active" id="panel1">
<h3 style="margin-top: 0;">Powerful new insights with epigenetic data mining.<br /> A study to distinguish smokers from non-smokers using just one droplet of blood</h3>
<p>Next generation sequencing in combination with sophisticated bioinformatics technologies for genomic, transcriptomic and epigeneomic analyses have enormous potential to establish new biomarkers for disease diagnostics, enabling true precision medicine. Analyses of liquid biopsies to measure thousands of different data points simultaneously in easily accessible body fluids (e.g. blood, urine, and saliva) are extremely promising for such biomarker studies.<br />(...)</p>
<a href="https://www.diagenode.com/en/pages/form-bioinfo" class="alert small button" target="_blank">Read more</a></div>
<div class="content" id="panel2">
<h3 style="margin-top: 0;">Data mining on DNA methylation data in cancer samples<br />Distinguishing normal from breast cancer tissue</h3>
<p>Breast cancer is the most commonly occurring cancer in women and the second most common cancer overall.</p>
<p>One important aspect of cancer tissues it that they differ from normal tissues in their epigenetic make up, especially in the DNA methylation pattern. In normal cells methylation assures the proper regulation of gene expression and stable gene silencing. DNA methylation is associated with histone modifications, and the interplay of these epigenetic modifications is crucial to regulate the functioning of the genome by changing chromatin architecture.<br />(...)</p>
<a href="https://www.diagenode.com/en/pages/form-bioinfo?app_note=23" class="alert small button" target="_blank">Read more</a></div>
</div>
</blockquote>
</div>
</div>
<p>Diagenode's new data mining service utilizes methods at the frontier of machine learning, statistics, and database systems. This enhanced service supports new discoveries that were previously not possible by analyzing patterns in large data sets to give informative new insights.</p>
<p>If you have data from patient cohorts, single cell analyses or any other heterogeneous scenarios, our service team provides enhanced support with optimal data analysis using our latest data mining capabilities. Specifically, our team applies machine learning technologies to find previously undiscovered or unobvious relationships within and across datasets. This advanced technology allows discovery of informative features from mass data, essentially &ldquo;finding a needle in a haystack.&rdquo;</p>
<p>Diagenode utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data. Our goal is to build strong classifiers that separate data into two or more classes or groups depending on associated data.</p>
<p class="extra-spaced">Different and multiple -omics data classes can be mined simultaneously. Integration with phenotypic and/or clinical data is also possible. We offer data mining services for several data classes including:</p>
<table class="extra-spaced">
<tbody>
<tr>
<td><strong>Epigenetic data</strong></td>
<td><strong>Transcriptomic data</strong></td>
</tr>
<tr>
<td>
<p>DNA Methylation (RRBS, WGBS, EPIC arrays)</p>
<p>ChIP-sequencing</p>
<p>ATAC-seq</p>
</td>
<td>
<p>mRNA-sequencing</p>
<p>Small and long non coding RNA</p>
<p>Single-cell RNA-sequencing</p>
</td>
</tr>
</tbody>
</table>
<p></p>
<p><strong>Biological Interpretation</strong></p>
<p class="extra-spaced">Machine learning classifiers also mirror the underlying biological differences between classes and are used to uncover the molecular processes at work. In order to achieve this, we offer biological interpretation services and pathway mining analyses for your data.</p>
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<li>Assessment of data characteristics and <span></span>applicability of different machine learning (ML)</li>
<li>Prototypic analyses:</li>
<li>Initial feasibility report</li>
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<p></p>
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<li><strong>Data Mining</strong></li>
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			'info2' => '<p>A clear advantage of <strong>Diagenode&rsquo;s data mining services</strong> is the close connection with<span>&nbsp; </span>other service offerings like <strong>wet lab analysis services</strong> and <strong>bioinformatics services</strong>. You can retrieve a full service package from a single source.</p>
<p><a href="https://www.diagenode.com/en/categories/Services">Read about our wetlab services</a></p>
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			'slug' => 'data-mining-service',
			'meta_title' => 'Bioinformatics Data Mining Service | Diagenode',
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			'meta_description' => 'Diagenode Data Mining Services utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data.',
			'modified' => '2020-12-21 08:05:15',
			'created' => '2019-04-08 16:43:26',
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		(int) 1 => array(
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			'name' => 'mRNA-seq Service',
			'description' => '<p><strong>mRNA-seq</strong> is the method of choice to efficiently detect and precisely quantify coding RNAs present in a biological sample under specific circumstances. Accurate estimation of transcript abundance allows for differential expression analysis as well as the study of alternative splicing and discovery of new transcript isoforms.</p>
<p>Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of mRNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on specific poly A selection to produce Illumina compatible libraries enriched in mRNAs.</p>
<h2>High performance mRNA-seq</h2>
<ul class="square">
<li>Superior complexity and high transcript coverage</li>
<li>Accurate comparison of gene expression levels</li>
<li>Detection of novel transcripts and alternative splicing events</li>
<li>Suitable for a broad range of input amounts</li>
<li>Compatible with FFPE samples</li>
<li>Fast turnaround time (from 5 to 6 weeks)</li>
</ul>
<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/rna-seq-category">See our other RNA-seq Profiling Services</a></p>',
			'label1' => 'Sample Requirements',
			'info1' => '<div class="extra-spaced">
<ul>
<li><strong>Project size</strong>: minimum of 24 samples</li>
<li><strong>Sample type</strong>: total isolated RNA</li>
<li><strong>Required quantities</strong>: minimum 10 ng of total RNA</li>
<li><strong>Shipment conditions</strong>: shipped in 1.5 mL or 0.5 mL tubes on dry ice</li>
</ul>
</div>',
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<div class="extra-spaced">
<h3 class="green">What our service includes</h3>
<ol>
<li><strong>RNA quality control</strong></li>
<ul>
<li>Determination of RIN value (RNA integration number)</li>
<li>Measurement of RNA concentration</li>
</ul>
<li><strong>Library preparation</strong></li>
<ul>
<li>Poly-A selection</li>
<li>Stranded library construction</li>
<li>Quality control of libraries prior to sequencing</li>
</ul>
<li><strong>Sequencing on Illumina platforms</strong></li>
<ul>
<li>25-30 million raw reads per sample</li>
</ul>
<li><strong>Data analysis</strong> (optional for additional fee)</li>
<ul>
<li>Standard bioinformatic analysis</li>
<li>Advanced bioinformatic analysis</li>
<li>Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request</li>
</ul>
</ol>
</div>',
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<td>
<h4 class="text-center"><strong>Standard bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
<td>
<h4 class="text-center"><strong>Advanced bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
</tr>
<tr>
<td style="vertical-align: top; padding-left: 20px;">
<ul>
<li>Demultiplexing, read quality control, trimming and filtering</li>
<li>Alignment to reference genome</li>
<li>Quantification of known transcripts</li>
</ul>
<br />
<p><em>Provided files:</em></p>
<ul>
<li>Report with sequencing statistics</li>
<li>Raw data in FASTQ format</li>
<li>FastQC report</li>
<li>Alignment in BAM format</li>
<li>Un-normalized read counts</li>
</ul>
</td>
<td style="vertical-align: top;">
<ul>
<li>Differential expression analysis</li>
<li>Gene ontology terms analysis</li>
<li>Pathway analysis</li>
<li>Alternative splicing analysis</li>
<li>Novel transcript identification</li>
</ul>
</td>
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			'slug' => 'mrna-seq-services',
			'meta_title' => 'mRNA-seq Service - Coding Transcriptome Analysis| Diagenode',
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			'meta_description' => 'mRNA-seq provides a complete view of the coding transcriptome. mRNA-Seq detects known and novel transcripts and measures transcript abundance for accurate, comprehensive analysis.',
			'modified' => '2020-12-24 12:47:26',
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			'id' => '3089',
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			'name' => 'Small RNA-seq Service',
			'description' => '<p><strong>Small RNA-seq</strong> is the method of choice to investigate the regulatory network of small non-coding RNAs (&lt;200 nt length), including but not limited to miRNA transcripts. It utilizes the proprietary technology D-Plex which ensures realistic representation of diverse small non-coding RNA transcripts with minimized bias. This approach is optimal for finding biomarkers from known transcripts and discover new RNA features.</p>
<p>Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of small RNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on a template-switching technology to produce Illumina compatible libraries enriched with the large variety of small non-coding RNAs.</p>
<h2>High transcript diversity with small RNA-seq</h2>
<ul class="square">
<li>Realistic representation of all small non-coding RNAs</li>
<li>Detection of novel transcripts and differentially-expressed small RNAs</li>
<li>Investigation of post-transcriptional regulation of gene expression</li>
<li>Leveraging the <a href="https://www.diagenode.com/en/pages/dplex">D-Plex technology</a></li>
<li>Working with ultra-low RNA inputs</li>
<li>Compatible with biological fluids</li>
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<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/rna-seq-category">See our other RNA-seq Profiling Services</a></p>',
			'label1' => 'Sample Requirements',
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<ul>
<li><strong>Project size</strong>: no minimum</li>
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<li><strong>Required quantities</strong>: minimum 100 pg of total RNA or 10 pg of small RNA</li>
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<ul>
<li>Standard bioinformatic analysis</li>
<li>Advanced bioinformatic analysis</li>
<li>Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request</li>
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			'label3' => 'Bioinformatics Analysis',
			'info3' => '<table style="padding: 40px;">
<tbody>
<tr>
<td>
<h4 class="text-center"><strong>Standard bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
<td>
<h4 class="text-center"><strong>Advanced bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
</tr>
<tr>
<td style="vertical-align: top; padding-left: 20px;">
<ul>
<li>Demultiplexing, read quality control, trimming and filtering</li>
<li>Alignment to reference genome</li>
<li>Quantification of small RNA biotypes with specialized quantification tool MGCount (<a href="https://doi.org/10.1186/s12859-021-04544-3">software developed by Diagenode</a>)</li>
</ul>
<br />
<p><em>Provided files:</em></p>
<ul>
<li>Report with sequencing statistics</li>
<li>Raw data in FASTQ format</li>
<li>FastQC report</li>
<li>Alignment in BAM format</li>
<li>Un-normalized read counts</li>
</ul>
</td>
<td style="vertical-align: top;">
<ul>
<li>Differential expression analysis</li>
<li>Gene ontology terms analysis</li>
<li>Pathway analysis</li>
</ul>
</td>
</tr>
</tbody>
</table>
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			'slug' => 'small-rna-seq-services',
			'meta_title' => 'Small RNA-seq Service - Small RNA and miRNA profiling | Diagenode',
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			'meta_description' => 'Small RNA-seq enables small RNA discovery, mutation characterization, and expression profiling of small RNAs by leveraging of sequencing technologies and data analysis pipeline.',
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			'name' => 'RNA Data Analysis',
			'description' => '<p>Total RNA sequencing (RNA-Seq) detects both coding and noncoding RNAs and is typically used to measure gene and transcript abundance as well as to identify novel components of the transcriptome. Messenger RNA-Seq focuses on the quantification of gene expression, the identification of unknown transcripts, the discovery of alternative splicing and gene fusion events. And finally, small non-coding RNA sequencing (sncRNA-Seq) will detect small (&lt;100 nucleotides long) RNAs that operate as key regulators in cellular processes.</p>
<div class="extra-spaced">
<h2>What do we provide with the analysis?</h2>
<p>This analysis provides information for either genes or isoforms with their expression levels.</p>
<h3 class="diacol" style="font-weight: 100;">Standard Analysis</h3>
<ul style="list-style-type: circle;">
<li>Summary statistics (total sequenced reads, total mapping reads, uniquely aligned reads, PCR duplicates, number of genes detected, average read density per gene, number of highly expressed genes, etc.)</li>
<li>Trimmed and filtered reads in fastQ files after sequencing QC</li>
<li>BAM sorted files from alignment to reference genome or transcriptome (indexed bam files and bigwig files included)</li>
<li>Matrix with expression abundance obtained with specialized quantification tool MGCount (<a href="https://doi.org/10.1186/s12859-021-04544-3">software developed by Diagenode</a>). A table of MG communities linking each original feature in the GTF file with the resultant count matrix and metadata feature identifiers.</li>
</ul>
<h3 class="diacol" style="font-weight: 100;">Advanced Analysis</h3>
<ul style="list-style-type: circle;">
<li>Comparative analysis (also called differential analysis) aimed at finding differentially expressed genes (DEGs) between two groups of samples</li>
<li>Functional gene annotation</li>
<li>Gene ontology enrichment analysis on DEGs</li>
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<h3 class="diacol" style="font-weight: 100;">Customized Analysis</h3>
<p class="text-left">If you require a type of analysis that is not in the previous list, <a href="#" data-reveal-id="quoteModal-3062">please consult with our expert bioinformatics team</a>.</p>
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<div><center><img src="https://www.diagenode.com/img/product/services/RNA-theorie.png" /></center></div>
<p><a href="https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-021-04544-3">MGcount</a>: Diagenode has developed a bioinformatics software for counting whole-transcriptome RNA-seq reads from one or more input alignment files. It is specially designed to incorporate multi-mapping and multi-overlapping reads in the quantification using a flexible methodology that is compatible with any biotype. At the end of its execution, it produces a count matrix, compatible with any downstream analysis.</p>
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<p>Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis.&nbsp;</p>
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<h3><span class="green">Chromatin</span>&nbsp;analysis</h3>
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<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/chip-seq-service">Learn more</a></p>
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<h3><span class="darkgrey">DNA methylation</span> analysis</h3>
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<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/dna-methylation-profiling-services">Learn more</a></p>
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<h3><span class="diacol"><g class="gr_ gr_44 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="44" data-gr-id="44">RNA-seq</g></span> analysis</h3>
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<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/rna-seq-category">Learn more</a></p>
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<h2 style="text-align: center;"><a class="details radius small button" href="https://www.diagenode.com/en/documents/services-flyer">DOWNLOAD OUR FLYER</a></h2>
<h2>Why Diagenode</h2>
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<li><strong>Innovative technology</strong>: Utilization of the signature Bioruptor<sup>&reg;</sup> sonication device for optimal chromatin and DNA shearing and the IP-Star<sup>&reg;</sup> Automation device give reproducible and reliable optimization and results</li>
<li><strong>Quality</strong>: Multiple QC steps in all workflows and validated antibodies plus reagents deliver superior data</li>
<li><strong>Flexibility</strong>: Extensive range of sample species and sample origins</li>
<li><strong>Experience</strong> in epigenomics profiling for a wide range of applications and fields of interest</li>
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<h2>12 years of expertise and trust in epigenetics</h2>
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<li><strong>End-to-end</strong> epigenetic service</li>
<li><strong>Collaborative</strong> and customized project design</li>
<li>Dedicated <strong>in-house expert</strong> for your project</li>
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<p><br />RNA-seq is a revolutionary technique that examines the total cellular content of RNAs, the transcriptome, using deep-sequencing technologies. Obtaining data about the transcriptome allows to connect the information on a genome with its functional protein expression. It provides great insights into cell development mechanisms and disease-associated modifications.</p>
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Our Epigenomics Profiling Services helps you to complete your transcriptomic profiling analysis using next-generation sequencing (NGS). Our experienced scientists will help you design your RNA-seq experiments based on the ultimate goal of your transcriptomic profiling analysis by generating meaningful RNA-seq libraries and delivering high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</div>
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<p>Epigenetics is crucial for the regulation of gene expression and has broad relevance in biological processes like development, disease and response to the environment. For more than 10 years Diagenode has been developing innovative tools to study epigenetic marks such as post-translational modi cations of histones and DNA methylation. We are now utilizing our expertise by offering custom services. Our <a href="https://www.diagenode.com/en/categories/Services">services</a> include full work ows for ChIP-sequencing as well as reduced representation bisul te sequencing (RRBS) with our new optimized &ldquo;Premium RRBSTM technology. In addition, we also offer bioinformatic analysis of your results, both standard and customized. The <a href="https://www.diagenode.com/en/categories/Services">Diagenode Epigenetics Custom Services</a> helps you to complete your epigenetics work ow from your starting biological material to your nal results.</p>
<p><a href="https://www.diagenode.com/en/categories/Services">Learn more</a></p>',
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			'description' => '<h6>Background and purpose</h6>
<div class="section-paragraph">Traumatic brain injury (TBI) and its consequences remain great challenges for neurology. Consequences of TBI are associated with various alterations in the brain but little is known about long-term changes of epigenetic DNA methylation patterns. Moreover, nothing is known about potential treatments that can alter these epigenetic changes in beneficial ways. Therefore, we have examined myo-inositol (MI), which has positive effects on several pathological conditions.</div>
<h6></h6>
<h6>Methods</h6>
<div class="section-paragraph">TBI was induced in mice by controlled cortical impact (CCI). One group of CCI animals received saline injections for two months (TBI+SAL), another CCI group received MI treatment (TBI+MI) for the same period and one group served as a sham-operated control. Mice were sacrificed 4 months after CCI and changes in DNA methylome and transcriptomes were examined.</div>
<h6></h6>
<h6>Results</h6>
<div class="section-paragraph">For the first time we: (i) provide comprehensive map of long-term DNA methylation changes after CCI in the hippocampus; (ii) identify differences by methylation sites between the groups; (iii) characterize transcriptome changes; (iv) provide association between DNA methylation sites and gene expression. MI treatment is linked with upregulation of genes covering 33 biological processes, involved in immune response and inflammation. In support of these findings, we have shown that expression of BATF2, a transcription factor involved in immune-regulatory networks, is upregulated in the hippocampus of the TBI+MI group where the BATF2 gene is demethylated.</div>
<h6></h6>
<h6>Conclusion</h6>
<div class="section-paragraph">TBI is followed by long-term epigenetic and transcriptomic changes in hippocampus. MI treatment has a significant effect on these processes by modulation of immune response and biological pathways of inflammation.</div>',
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                         <a href="/cn/p/data-mining-service"><img src="https://www.diagenode.com/img/product/data-mining.jpg" alt="Bioinformatics Data Mining Service" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">G02100000</span>
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            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
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        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioinformatics Data Mining Service</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

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<div class="row collapse">
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<!------------SERVICES PARTICULAR FORM START---------------->


<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->


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            <h6 style="height:60px">Bioinformatics Data Mining Service</h6>
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                         <a href="/cn/p/mrna-seq-services"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



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<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->


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<a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: QUOTE MODAL --><a href="#" id="small-rna-seq-services" data-reveal-id="quoteModal-3089" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Small RNA-seq Services</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/cn/p/rna-data-analysis"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



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        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>RNA Data Analysis</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

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	'description' => '<p><span>Inflammatory stresses underlie endothelial dysfunction and contribute to the development of chronic cardiovascular disorders such as atherosclerosis and vascular fibrosis. The initial transcriptional response of endothelial cells to pro-inflammatory cytokines such as TNF-alpha is well established. However, very few studies uncover the effects of inflammatory stresses on chromatin architecture. We used integrative analysis of ATAC-seq and RNA-seq data to investigate chromatin alterations in human endothelial cells in response to TNF-alpha and febrile-range heat stress exposure. Multi-omics data analysis suggests a correlation between the transcription of stress-related genes and endothelial dysfunction drivers with chromatin regions exhibiting differential accessibility. Moreover, microscopy identified the dynamics in the nuclear organization, specifically, the changes in a subset of heterochromatic nucleoli-associated chromatin domains, the centromeres. Upon inflammatory stress exposure, the centromeres decreased association with nucleoli in a p38-dependent manner and increased the number of transcripts from pericentromeric regions. Overall, we provide two lines of evidence that suggest chromatin alterations in vascular endothelial cells during inflammatory stresses.</span></p>',
	'date' => '2023-10-16',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614786/',
	'doi' => '10.1101/2023.10.11.561959',
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Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]
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			'description' => '<h2 class="text-center"><span class="diacol">New!</span> <br />Data mining using machine learning (AI) for unique epigenetic data insights</h2>
<center><img src="https://www.diagenode.com/img/product/data-mining-long.png" /></center>
<p></p>
<div id="paper" style="text-align: center;">
<h4><a href="https://www.youtube.com/watch?v=KXjnSHz3Jk8">Watch the webinar to gain insights on how data mining can be applied for epigenetics applications</a></h4>
</div>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<blockquote style="padding-bottom: 0;"><span class="label-green" style="margin-bottom: 16px; margin-left: -22px; font-size: 22px;">WHITE PAPERS</span>
<div id="portal" class="main-portal">
<div class="portal-inner"><nav class="portal-nav" style="text-align: left;">
<ul data-tab="" class="tips-menu">
<li><a href="#panel1" class="tips portal button">Smokers vs non-smokers </a></li>
<li><a href="#panel2" class="tips portal button">Breast cancer</a></li>
</ul>
</nav></div>
</div>
<div class="tabs-content">
<div class="content active" id="panel1">
<h3 style="margin-top: 0;">Powerful new insights with epigenetic data mining.<br /> A study to distinguish smokers from non-smokers using just one droplet of blood</h3>
<p>Next generation sequencing in combination with sophisticated bioinformatics technologies for genomic, transcriptomic and epigeneomic analyses have enormous potential to establish new biomarkers for disease diagnostics, enabling true precision medicine. Analyses of liquid biopsies to measure thousands of different data points simultaneously in easily accessible body fluids (e.g. blood, urine, and saliva) are extremely promising for such biomarker studies.<br />(...)</p>
<a href="https://www.diagenode.com/en/pages/form-bioinfo" class="alert small button" target="_blank">Read more</a></div>
<div class="content" id="panel2">
<h3 style="margin-top: 0;">Data mining on DNA methylation data in cancer samples<br />Distinguishing normal from breast cancer tissue</h3>
<p>Breast cancer is the most commonly occurring cancer in women and the second most common cancer overall.</p>
<p>One important aspect of cancer tissues it that they differ from normal tissues in their epigenetic make up, especially in the DNA methylation pattern. In normal cells methylation assures the proper regulation of gene expression and stable gene silencing. DNA methylation is associated with histone modifications, and the interplay of these epigenetic modifications is crucial to regulate the functioning of the genome by changing chromatin architecture.<br />(...)</p>
<a href="https://www.diagenode.com/en/pages/form-bioinfo?app_note=23" class="alert small button" target="_blank">Read more</a></div>
</div>
</blockquote>
</div>
</div>
<p>Diagenode's new data mining service utilizes methods at the frontier of machine learning, statistics, and database systems. This enhanced service supports new discoveries that were previously not possible by analyzing patterns in large data sets to give informative new insights.</p>
<p>If you have data from patient cohorts, single cell analyses or any other heterogeneous scenarios, our service team provides enhanced support with optimal data analysis using our latest data mining capabilities. Specifically, our team applies machine learning technologies to find previously undiscovered or unobvious relationships within and across datasets. This advanced technology allows discovery of informative features from mass data, essentially &ldquo;finding a needle in a haystack.&rdquo;</p>
<p>Diagenode utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data. Our goal is to build strong classifiers that separate data into two or more classes or groups depending on associated data.</p>
<p class="extra-spaced">Different and multiple -omics data classes can be mined simultaneously. Integration with phenotypic and/or clinical data is also possible. We offer data mining services for several data classes including:</p>
<table class="extra-spaced">
<tbody>
<tr>
<td><strong>Epigenetic data</strong></td>
<td><strong>Transcriptomic data</strong></td>
</tr>
<tr>
<td>
<p>DNA Methylation (RRBS, WGBS, EPIC arrays)</p>
<p>ChIP-sequencing</p>
<p>ATAC-seq</p>
</td>
<td>
<p>mRNA-sequencing</p>
<p>Small and long non coding RNA</p>
<p>Single-cell RNA-sequencing</p>
</td>
</tr>
</tbody>
</table>
<p></p>
<p><strong>Biological Interpretation</strong></p>
<p class="extra-spaced">Machine learning classifiers also mirror the underlying biological differences between classes and are used to uncover the molecular processes at work. In order to achieve this, we offer biological interpretation services and pathway mining analyses for your data.</p>
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<p><a href="https://www.diagenode.com/en/categories/Services">Read about our wetlab services</a></p>
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			'slug' => 'data-mining-service',
			'meta_title' => 'Bioinformatics Data Mining Service | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'Diagenode Data Mining Services utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data.',
			'modified' => '2020-12-21 08:05:15',
			'created' => '2019-04-08 16:43:26',
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		(int) 1 => array(
			'id' => '3088',
			'antibody_id' => null,
			'name' => 'mRNA-seq Service',
			'description' => '<p><strong>mRNA-seq</strong> is the method of choice to efficiently detect and precisely quantify coding RNAs present in a biological sample under specific circumstances. Accurate estimation of transcript abundance allows for differential expression analysis as well as the study of alternative splicing and discovery of new transcript isoforms.</p>
<p>Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of mRNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on specific poly A selection to produce Illumina compatible libraries enriched in mRNAs.</p>
<h2>High performance mRNA-seq</h2>
<ul class="square">
<li>Superior complexity and high transcript coverage</li>
<li>Accurate comparison of gene expression levels</li>
<li>Detection of novel transcripts and alternative splicing events</li>
<li>Suitable for a broad range of input amounts</li>
<li>Compatible with FFPE samples</li>
<li>Fast turnaround time (from 5 to 6 weeks)</li>
</ul>
<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/rna-seq-category">See our other RNA-seq Profiling Services</a></p>',
			'label1' => 'Sample Requirements',
			'info1' => '<div class="extra-spaced">
<ul>
<li><strong>Project size</strong>: minimum of 24 samples</li>
<li><strong>Sample type</strong>: total isolated RNA</li>
<li><strong>Required quantities</strong>: minimum 10 ng of total RNA</li>
<li><strong>Shipment conditions</strong>: shipped in 1.5 mL or 0.5 mL tubes on dry ice</li>
</ul>
</div>',
			'label2' => 'Services Workflow',
			'info2' => '<center><img alt="mRNA-seq Service" src="https://www.diagenode.com/img/product/services/mRNA-seq_workflow.png" /></center>
<div class="extra-spaced">
<h3 class="green">What our service includes</h3>
<ol>
<li><strong>RNA quality control</strong></li>
<ul>
<li>Determination of RIN value (RNA integration number)</li>
<li>Measurement of RNA concentration</li>
</ul>
<li><strong>Library preparation</strong></li>
<ul>
<li>Poly-A selection</li>
<li>Stranded library construction</li>
<li>Quality control of libraries prior to sequencing</li>
</ul>
<li><strong>Sequencing on Illumina platforms</strong></li>
<ul>
<li>25-30 million raw reads per sample</li>
</ul>
<li><strong>Data analysis</strong> (optional for additional fee)</li>
<ul>
<li>Standard bioinformatic analysis</li>
<li>Advanced bioinformatic analysis</li>
<li>Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request</li>
</ul>
</ol>
</div>',
			'label3' => 'Bioinformatics Analysis',
			'info3' => '<table style="padding: 40px;">
<tbody>
<tr>
<td>
<h4 class="text-center"><strong>Standard bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
<td>
<h4 class="text-center"><strong>Advanced bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
</tr>
<tr>
<td style="vertical-align: top; padding-left: 20px;">
<ul>
<li>Demultiplexing, read quality control, trimming and filtering</li>
<li>Alignment to reference genome</li>
<li>Quantification of known transcripts</li>
</ul>
<br />
<p><em>Provided files:</em></p>
<ul>
<li>Report with sequencing statistics</li>
<li>Raw data in FASTQ format</li>
<li>FastQC report</li>
<li>Alignment in BAM format</li>
<li>Un-normalized read counts</li>
</ul>
</td>
<td style="vertical-align: top;">
<ul>
<li>Differential expression analysis</li>
<li>Gene ontology terms analysis</li>
<li>Pathway analysis</li>
<li>Alternative splicing analysis</li>
<li>Novel transcript identification</li>
</ul>
</td>
</tr>
</tbody>
</table>
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			'slug' => 'mrna-seq-services',
			'meta_title' => 'mRNA-seq Service - Coding Transcriptome Analysis| Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'mRNA-seq provides a complete view of the coding transcriptome. mRNA-Seq detects known and novel transcripts and measures transcript abundance for accurate, comprehensive analysis.',
			'modified' => '2020-12-24 12:47:26',
			'created' => '2020-07-13 10:52:47',
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		(int) 2 => array(
			'id' => '3089',
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			'name' => 'Small RNA-seq Service',
			'description' => '<p><strong>Small RNA-seq</strong> is the method of choice to investigate the regulatory network of small non-coding RNAs (&lt;200 nt length), including but not limited to miRNA transcripts. It utilizes the proprietary technology D-Plex which ensures realistic representation of diverse small non-coding RNA transcripts with minimized bias. This approach is optimal for finding biomarkers from known transcripts and discover new RNA features.</p>
<p>Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of small RNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on a template-switching technology to produce Illumina compatible libraries enriched with the large variety of small non-coding RNAs.</p>
<h2>High transcript diversity with small RNA-seq</h2>
<ul class="square">
<li>Realistic representation of all small non-coding RNAs</li>
<li>Detection of novel transcripts and differentially-expressed small RNAs</li>
<li>Investigation of post-transcriptional regulation of gene expression</li>
<li>Leveraging the <a href="https://www.diagenode.com/en/pages/dplex">D-Plex technology</a></li>
<li>Working with ultra-low RNA inputs</li>
<li>Compatible with biological fluids</li>
</ul>
<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/rna-seq-category">See our other RNA-seq Profiling Services</a></p>',
			'label1' => 'Sample Requirements',
			'info1' => '<div class="extra-spaced">
<ul>
<li><strong>Project size</strong>: no minimum</li>
<li><strong>Sample type</strong>: total isolated RNA or small enriched RNA</li>
<li><strong>Required quantities</strong>: minimum 100 pg of total RNA or 10 pg of small RNA</li>
<li><strong>Shipment conditions</strong>: shipped in 1.5 mL or 0.5 mL tubes on dry ice</li>
</ul>
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			'label2' => 'Services Workflow',
			'info2' => '<center><img alt="Small RNA-seq Service" src="https://www.diagenode.com/img/product/services/sncRNA-seq_workflow.png" /></center>
<div class="extra-spaced">
<h3 class="green">What our service includes</h3>
<ol>
<li><strong>RNA quality control</strong></li>
<ul>
<li>Determination of RIN value (RNA integration number)</li>
<li>Measurement of RNA concentration</li>
</ul>
<li><strong>Library preparation</strong></li>
<ul>
<li>Capture and amplification by tailing and switching</li>
<li>Library construction</li>
<li>Quality control of libraries prior to sequencing</li>
</ul>
<li><strong>Sequencing on Illumina platforms</strong></li>
<ul>
<li>10-20 million raw reads per sample</li>
</ul>
<li><strong>Data analysis</strong> (optional for additional fee)</li>
<ul>
<li>Standard bioinformatic analysis</li>
<li>Advanced bioinformatic analysis</li>
<li>Data delivery of raw data and all generated files through fast and secure file transfer or hard drive upon request</li>
</ul>
</ol>
</div>',
			'label3' => 'Bioinformatics Analysis',
			'info3' => '<table style="padding: 40px;">
<tbody>
<tr>
<td>
<h4 class="text-center"><strong>Standard bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
<td>
<h4 class="text-center"><strong>Advanced bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
</td>
</tr>
<tr>
<td style="vertical-align: top; padding-left: 20px;">
<ul>
<li>Demultiplexing, read quality control, trimming and filtering</li>
<li>Alignment to reference genome</li>
<li>Quantification of small RNA biotypes with specialized quantification tool MGCount (<a href="https://doi.org/10.1186/s12859-021-04544-3">software developed by Diagenode</a>)</li>
</ul>
<br />
<p><em>Provided files:</em></p>
<ul>
<li>Report with sequencing statistics</li>
<li>Raw data in FASTQ format</li>
<li>FastQC report</li>
<li>Alignment in BAM format</li>
<li>Un-normalized read counts</li>
</ul>
</td>
<td style="vertical-align: top;">
<ul>
<li>Differential expression analysis</li>
<li>Gene ontology terms analysis</li>
<li>Pathway analysis</li>
</ul>
</td>
</tr>
</tbody>
</table>
<p></p>',
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			'price_JPY' => '42800',
			'price_CNY' => 'on request',
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			'slug' => 'small-rna-seq-services',
			'meta_title' => 'Small RNA-seq Service - Small RNA and miRNA profiling | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'Small RNA-seq enables small RNA discovery, mutation characterization, and expression profiling of small RNAs by leveraging of sequencing technologies and data analysis pipeline.',
			'modified' => '2022-03-07 09:12:41',
			'created' => '2020-07-13 11:09:54',
			'ProductsRelated' => array(
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		(int) 3 => array(
			'id' => '3062',
			'antibody_id' => null,
			'name' => 'RNA Data Analysis',
			'description' => '<p>Total RNA sequencing (RNA-Seq) detects both coding and noncoding RNAs and is typically used to measure gene and transcript abundance as well as to identify novel components of the transcriptome. Messenger RNA-Seq focuses on the quantification of gene expression, the identification of unknown transcripts, the discovery of alternative splicing and gene fusion events. And finally, small non-coding RNA sequencing (sncRNA-Seq) will detect small (&lt;100 nucleotides long) RNAs that operate as key regulators in cellular processes.</p>
<div class="extra-spaced">
<h2>What do we provide with the analysis?</h2>
<p>This analysis provides information for either genes or isoforms with their expression levels.</p>
<h3 class="diacol" style="font-weight: 100;">Standard Analysis</h3>
<ul style="list-style-type: circle;">
<li>Summary statistics (total sequenced reads, total mapping reads, uniquely aligned reads, PCR duplicates, number of genes detected, average read density per gene, number of highly expressed genes, etc.)</li>
<li>Trimmed and filtered reads in fastQ files after sequencing QC</li>
<li>BAM sorted files from alignment to reference genome or transcriptome (indexed bam files and bigwig files included)</li>
<li>Matrix with expression abundance obtained with specialized quantification tool MGCount (<a href="https://doi.org/10.1186/s12859-021-04544-3">software developed by Diagenode</a>). A table of MG communities linking each original feature in the GTF file with the resultant count matrix and metadata feature identifiers.</li>
</ul>
<h3 class="diacol" style="font-weight: 100;">Advanced Analysis</h3>
<ul style="list-style-type: circle;">
<li>Comparative analysis (also called differential analysis) aimed at finding differentially expressed genes (DEGs) between two groups of samples</li>
<li>Functional gene annotation</li>
<li>Gene ontology enrichment analysis on DEGs</li>
<li>Pathway enrichment analysis on DEGs (KEGG or DOSE for human samples)</li>
<li>Alternative splicing analysis</li>
<li>Gene fusion analysis</li>
<li>Novel transcript identification</li>
</ul>
<h3 class="diacol" style="font-weight: 100;">Customized Analysis</h3>
<p class="text-left">If you require a type of analysis that is not in the previous list, <a href="#" data-reveal-id="quoteModal-3062">please consult with our expert bioinformatics team</a>.</p>
</div>
<div><center><img src="https://www.diagenode.com/img/product/services/RNA-theorie.png" /></center></div>
<p><a href="https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-021-04544-3">MGcount</a>: Diagenode has developed a bioinformatics software for counting whole-transcriptome RNA-seq reads from one or more input alignment files. It is specially designed to incorporate multi-mapping and multi-overlapping reads in the quantification using a flexible methodology that is compatible with any biotype. At the end of its execution, it produces a count matrix, compatible with any downstream analysis.</p>
<center><img src="https://www.diagenode.com/img/product/services/mGcount-img.png" width="600px" /></center>',
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			'price_EUR' => '/',
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			'price_JPY' => '42800',
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			'price_AUD' => '/',
			'country' => 'ALL',
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			'online' => true,
			'master' => true,
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			'slug' => 'rna-data-analysis',
			'meta_title' => 'RNA Data Analysis Services | Diagenode',
			'meta_keywords' => '',
			'meta_description' => 'RNA-seq data analysis service is made to extract information from a large variety of  RNA-seq data (small RNA, mRNA and much more).',
			'modified' => '2022-11-22 15:26:24',
			'created' => '2020-03-26 10:04:58',
			'ProductsRelated' => array(
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	'Application' => array(
		(int) 0 => array(
			'id' => '51',
			'position' => '0',
			'parent_id' => '46',
			'name' => '小型RNAとmiRNA',
			'description' => '<ul>
<li><span> </span>遺伝子発現の転写後調節</li>
<li><span> </span>新規転写物および差示的に発現される小RNA</li>
<li><span> </span>腫瘍抑制遺伝子発現の調節に関与するmiRNA</li>
<li><span> </span>差次的miRNA発現</li>
</ul>',
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			'slug' => 'small-rna-and-mirna',
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			'meta_description' => '',
			'meta_title' => '',
			'modified' => '2018-01-12 15:03:17',
			'created' => '2017-11-20 16:22:17',
			'ProductsApplication' => array(
				[maximum depth reached]
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		(int) 1 => array(
			'id' => '52',
			'position' => '0',
			'parent_id' => '46',
			'name' => 'メッセンジャーRNA(ｍRNA)',
			'description' => '<ul>
<li><span> </span>特異的ポリA選定で<span>タンパク質コーディング</span><span>RNA</span><span></span><span></span></li>
<li><span> </span>がん関連mRNAシグネチャー</li>
<li><span> </span>癌の病因における異常なmRNA転換</li>
<li><span> </span>遺伝子発現プロファイリングおよび示差的に発現される遺伝子の同定（DEG）</li>
<li><span> </span>遺伝子発現のエピジェネティックな機構（トランスクリプトーム+ H3K36me3およびH3K4me3標識）</li>
<li><span> </span>コーディング遺伝子の機能的アノテーション</li>
</ul>',
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			'slug' => 'messenge-rna-mRNA',
			'meta_keywords' => '',
			'meta_description' => '',
			'meta_title' => '',
			'modified' => '2018-01-12 16:17:37',
			'created' => '2017-11-20 16:23:45',
			'ProductsApplication' => array(
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		(int) 2 => array(
			'id' => '53',
			'position' => '0',
			'parent_id' => '46',
			'name' => '全トランスクリプトーム解析と長い非コードRNA（lncRNA）解析',
			'description' => '<ul>
<li><span> </span>トランスクリプトーム配列決定：任意のrRNA<span>除去</span>を伴う200ntより大きい<span>RNA</span><span>および</span>全トランスクリプトーム<span></span><span></span>の分析</li>
<li><span> </span>クロマチンリモデリングおよび転写/転写後調節に関与する非タンパク質コードRNA</li>
</ul>
<p><span>&nbsp;</span></p>',
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			'slug' => 'whole-transcriptome-analysis-and-long-non-coding-rna-incrna-analysis',
			'meta_keywords' => '',
			'meta_description' => '',
			'meta_title' => '',
			'modified' => '2018-01-12 16:29:08',
			'created' => '2017-11-20 16:25:22',
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	'Category' => array(
		(int) 0 => array(
			'id' => '121',
			'position' => '9',
			'parent_id' => null,
			'name' => 'Epigenomics Profiling Services',
			'description' => '<div class="row">
<div class="small-12 medium-10 large-10 columns text-left">
<p>Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis.&nbsp;</p>
</div>
<div class="small-12 medium-2 large-2 columns text-left"><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></div>
</div>
<center><img alt="DNA-RNA-Chromatin-Diagenode" src="https://www.diagenode.com/img/categories/services/chromatin-dna-rna.png" /></center>
<div class="row">
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #099f92;">
<h3><span class="green">Chromatin</span>&nbsp;analysis</h3>
<ul>
<li>ChIP-seq analysis Histone modification</li>
<li>Promoter analysis</li>
<li>Enhancer analysis</li>
<li>ChIP-seq analysis Transcription factor&nbsp;</li>
<li>(sc)ATAC-seq Chromatin accessibility</li>
<li>ChIP-seq Cas9 off-target</li>
<li>Customized NGS service</li>
</ul>
<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/chip-seq-service">Learn more</a></p>
</div>
</div>
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #474546;">
<h3><span class="darkgrey">DNA methylation</span> analysis</h3>
<ul>
<li>Reduced representation bisulfite sequencing (RRBS)</li>
<li>Whole genome bisulfite sequencing (WGBS)</li>
<li>Targeted methylation</li>
<li>MeDIP-seq/hMeDIP-seq</li>
<li>Infinium MethylationEPIC array&nbsp;</li>
</ul>
<p></p>
<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/dna-methylation-profiling-services">Learn more</a></p>
</div>
</div>
<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #b11e33;">
<h3><span class="diacol"><g class="gr_ gr_44 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="44" data-gr-id="44">RNA-seq</g></span> analysis</h3>
<ul>
<li><span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;">Gene expression profiling</span></li>
<li>mRNA<span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;"> analysis</span></li>
<li>Small non-coding RNA analysis</li>
<li><b></b>Whole transcriptome analysis<br /><br /><br /><br /><br /></li>
</ul>
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<li><strong>Expertise and trust</strong>: Recognized epigenetics leader, official partner of <a href="http://www.blueprint-epigenome.eu/"><g class="gr_ gr_45 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="45" data-gr-id="45">BLUEPRINT</g></a>, <a href="http://ihec-epigenomes.org/">IHEC</a> <g class="gr_ gr_46 gr-alert gr_gramm gr_inline_cards gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="46" data-gr-id="46">and</g> <a href="https://www.faang.org/">FAANG</a></li>
<li><strong>Innovative technology</strong>: Utilization of the signature Bioruptor<sup>&reg;</sup> sonication device for optimal chromatin and DNA shearing and the IP-Star<sup>&reg;</sup> Automation device give reproducible and reliable optimization and results</li>
<li><strong>Quality</strong>: Multiple QC steps in all workflows and validated antibodies plus reagents deliver superior data</li>
<li><strong>Flexibility</strong>: Extensive range of sample species and sample origins</li>
<li><strong>Experience</strong> in epigenomics profiling for a wide range of applications and fields of interest</li>
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<h2>12 years of expertise and trust in epigenetics</h2>
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<li><strong>End-to-end</strong> epigenetic service</li>
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<div class="panel"><center><img alt="RNA-seq-Diagenode" src="http://www.diagenode.com/img/categories/services/rna-workflow.png" /></center>
<p><br />RNA-seq is a revolutionary technique that examines the total cellular content of RNAs, the transcriptome, using deep-sequencing technologies. Obtaining data about the transcriptome allows to connect the information on a genome with its functional protein expression. It provides great insights into cell development mechanisms and disease-associated modifications.</p>
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<center><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></center></div>
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<h3><a href="https://www.diagenode.com/en/p/mrna-seq-services">mRNA analysis</a></h3>
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Our Epigenomics Profiling Services helps you to complete your transcriptomic profiling analysis using next-generation sequencing (NGS). Our experienced scientists will help you design your RNA-seq experiments based on the ultimate goal of your transcriptomic profiling analysis by generating meaningful RNA-seq libraries and delivering high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</div>
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<p>Epigenetics is crucial for the regulation of gene expression and has broad relevance in biological processes like development, disease and response to the environment. For more than 10 years Diagenode has been developing innovative tools to study epigenetic marks such as post-translational modi cations of histones and DNA methylation. We are now utilizing our expertise by offering custom services. Our <a href="https://www.diagenode.com/en/categories/Services">services</a> include full work ows for ChIP-sequencing as well as reduced representation bisul te sequencing (RRBS) with our new optimized &ldquo;Premium RRBSTM technology. In addition, we also offer bioinformatic analysis of your results, both standard and customized. The <a href="https://www.diagenode.com/en/categories/Services">Diagenode Epigenetics Custom Services</a> helps you to complete your epigenetics work ow from your starting biological material to your nal results.</p>
<p><a href="https://www.diagenode.com/en/categories/Services">Learn more</a></p>',
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<li>Chromatin analysis</li>
<li>DNA methylation services</li>
<li>RNA-seq analysis</li>
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			'description' => '<h6>Background and purpose</h6>
<div class="section-paragraph">Traumatic brain injury (TBI) and its consequences remain great challenges for neurology. Consequences of TBI are associated with various alterations in the brain but little is known about long-term changes of epigenetic DNA methylation patterns. Moreover, nothing is known about potential treatments that can alter these epigenetic changes in beneficial ways. Therefore, we have examined myo-inositol (MI), which has positive effects on several pathological conditions.</div>
<h6></h6>
<h6>Methods</h6>
<div class="section-paragraph">TBI was induced in mice by controlled cortical impact (CCI). One group of CCI animals received saline injections for two months (TBI+SAL), another CCI group received MI treatment (TBI+MI) for the same period and one group served as a sham-operated control. Mice were sacrificed 4 months after CCI and changes in DNA methylome and transcriptomes were examined.</div>
<h6></h6>
<h6>Results</h6>
<div class="section-paragraph">For the first time we: (i) provide comprehensive map of long-term DNA methylation changes after CCI in the hippocampus; (ii) identify differences by methylation sites between the groups; (iii) characterize transcriptome changes; (iv) provide association between DNA methylation sites and gene expression. MI treatment is linked with upregulation of genes covering 33 biological processes, involved in immune response and inflammation. In support of these findings, we have shown that expression of BATF2, a transcription factor involved in immune-regulatory networks, is upregulated in the hippocampus of the TBI+MI group where the BATF2 gene is demethylated.</div>
<h6></h6>
<h6>Conclusion</h6>
<div class="section-paragraph">TBI is followed by long-term epigenetic and transcriptomic changes in hippocampus. MI treatment has a significant effect on these processes by modulation of immune response and biological pathways of inflammation.</div>',
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            </div>

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        <div class="small-12 columns" >
            <h6 style="height:60px">Bioinformatics Data Mining Service</h6>
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                         <a href="/cn/p/mrna-seq-services"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



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        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>mRNA-seq Service</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

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<a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: QUOTE MODAL --><a href="#" id="mrna-seq-services" data-reveal-id="quoteModal-3088" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a>
            </div>

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        <div class="small-12 columns" >
            <h6 style="height:60px">mRNA-seq Service</h6>
        </div>

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</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/cn/p/small-rna-seq-services"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



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        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Small RNA-seq Services</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

    </div>
</div>

<form action="/cn/quotes/quote?id=3089" id="Quote-3089" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="3089" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="N3hwZStYQTg3OTVBMjBwUldTaTNrZz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="small-rna-seq-services" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/>

    <div class="row collapse">
        <h2>Your project</h2>
    </div>

    <div class="small-12 large-12 columns">
        <h4>Which services are you interested in?</h4>
    </div>
    <div class="small-12 large-12 columns">
        <input type="hidden" name="data[Quote][rna_seq_service]" value="" id="QuoteRnaSeqService"/>

<div class="checkbox"><input type="checkbox" name="data[Quote][rna_seq_service][]" value="High-throughput 3mRNA-seq" id="QuoteRnaSeqServiceHighThroughput3mRNASeq" /><label for="QuoteRnaSeqServiceHighThroughput3mRNASeq">High-throughput 3mRNA-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][rna_seq_service][]" value="mRNA-seq" id="QuoteRnaSeqServiceMRNASeq" /><label for="QuoteRnaSeqServiceMRNASeq">mRNA-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][rna_seq_service][]" value="Small RNA-seq" id="QuoteRnaSeqServiceSmallRNASeq" /><label for="QuoteRnaSeqServiceSmallRNASeq">Small RNA-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][rna_seq_service][]" value="Total RNA-seq" id="QuoteRnaSeqServiceTotalRNASeq" /><label for="QuoteRnaSeqServiceTotalRNASeq">Total RNA-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][rna_seq_service][]" value="Bioinformatics" id="QuoteRnaSeqServiceBioinformatics" /><label for="QuoteRnaSeqServiceBioinformatics">Bioinformatics</label></div>
    </div>

    <div class="row collapse">
        <div class="small-12 medium-12 large-3 columns">
            <span class="prefix">Sample species</span>
        </div>
        <div class="small-12 medium-12 large-9 columns">
            <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/>        </div>
    </div>

    <div class="row collapse">
        <div class="small-12 medium-12 large-6 columns">
            <span class="prefix">Total number of samples (including replicates)</span>
        </div>
        <div class="small-12 medium-12 large-6 columns">
            <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/>        </div>
    </div>

<div class="row collapse">
    <h2>Contact Information</h2>
    <div class="small-3 large-2 columns">
        <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Phone number</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">City</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-3089">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d&#039;Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People&#039;s Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People&#039;s Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
<option value="SG">Singapore</option>
<option value="SX">Sint Maarten (Dutch part)</option>
<option value="SK">Slovakia</option>
<option value="SI">Slovenia</option>
<option value="SB">Solomon Islands</option>
<option value="SO">Somalia</option>
<option value="ZA">South Africa</option>
<option value="GS">South Georgia and the South Sandwich Islands</option>
<option value="ES">Spain</option>
<option value="LK">Sri Lanka</option>
<option value="SD">Sudan</option>
<option value="SR">Suriname</option>
<option value="SS">South Sudan</option>
<option value="SJ">Svalbard and Jan Mayen</option>
<option value="SZ">Swaziland</option>
<option value="SE">Sweden</option>
<option value="CH">Switzerland</option>
<option value="SY">Syrian Arab Republic</option>
<option value="TW">Taiwan</option>
<option value="TJ">Tajikistan</option>
<option value="TZ">Tanzania</option>
<option value="TH">Thailand</option>
<option value="TL">Timor-Leste</option>
<option value="TG">Togo</option>
<option value="TK">Tokelau</option>
<option value="TO">Tonga</option>
<option value="TT">Trinidad and Tobago</option>
<option value="TN">Tunisia</option>
<option value="TR">Turkey</option>
<option value="TM">Turkmenistan</option>
<option value="TC">Turks and Caicos Islands</option>
<option value="TV">Tuvalu</option>
<option value="UG">Uganda</option>
<option value="UA">Ukraine</option>
<option value="AE">United Arab Emirates</option>
<option value="GB">United Kingdom</option>
<option value="US" selected="selected">United States</option>
<option value="UM">United States Minor Outlying Islands</option>
<option value="UY">Uruguay</option>
<option value="UZ">Uzbekistan</option>
<option value="VU">Vanuatu</option>
<option value="VE">Venezuela</option>
<option value="VN">Viet Nam</option>
<option value="VG">Virgin Islands, British</option>
<option value="VI">Virgin Islands, U.S.</option>
<option value="WF">Wallis and Futuna</option>
<option value="EH">Western Sahara</option>
<option value="YE">Yemen</option>
<option value="ZM">Zambia</option>
<option value="ZW">Zimbabwe</option>
</select><script>
    $('#country_selector_quote-3089').selectize();
</script><br />
    </div>
</div>
<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">State</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][state]" id="state-3089" maxlength="3" type="text"/><br />
    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/>    </div>
</div>
<div class="row collapse" id="email_v">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/>    </div>
</div>


<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Comment</span>
    </div>
    <div class="small-9 large-10 columns">
        <textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea>    </div>
</div>

<!------------SERVICES PARTICULAR FORM START---------------->


<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->


<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <div class="recaptcha"><div id="recaptcha662a53be58d63"></div></div>    </div>
</div>
<br />

<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <button id="submit_btn-3089" class="alert button expand" form="Quote-3089" type="submit">Contact me</button>    </div>
</div>
</form><script>
    var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c';

    function postToPardot(formAction, id) {
        $('#pardot-form-handler').load(function(){
            $('#Quote-' + id).attr('action', formAction);
            $('#Quote-' + id).submit();
        });
        $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize());
        
    }
    $(document).ready(function() {
        $('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>');
		$('#Quote-3089').attr('action','javascript:postToPardot(\'' + $('#Quote-3089').attr('action') + '\', 3089)');
    });

    $("#Quote-3089 #submit_btn-3089").click(function (e) {
            
        if($(this).closest('form')[0].checkValidity()){
            e.preventDefault();
            
            //disable the submit button
            $("#Quote-3089 #submit_btn-3089").attr("disabled", true);
            $("#Quote-3089 #submit_btn-3089").html("Processing...");

            //submit the form
            $("#Quote-3089").submit();
        } 
    })
</script>
<script>
    if ($("#Quote-3089 #country_selector_quote-3089.selectized").val() == 'US') {
        var val = $("#state-3089").val();
        $("#Quote-3089 #state-3089").replaceWith('<select name="data[Quote][state]" id="state-3089" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
        if (val.length == 2) {
            $("#state-3089").val(val);
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        $("#state-3089").parent().parent().show();
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        var val = $("#state-3089").val();
        $("#Quote-3089 #state-3089").replaceWith('<select name="data[Quote][state]" id="state-3089" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
        if (val.length == 2) {
            $("#state-3089").val(val);
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        $("#state-3089").parent().parent().show();
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        var val = $("#state-3089").val();
        $("#Quote-3089 #state-3089").replaceWith('<select name="data[Quote][state]" id="state-3089" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
        if (val.length == 2) {
            $("#state-3089").val(val);
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        $("#Quote-3089 #state-3089").parent().parent().hide();
        $("#Quote-3089 #state-3089").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3089" value="">');
    }

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<a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: QUOTE MODAL --><a href="#" id="small-rna-seq-services" data-reveal-id="quoteModal-3089" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Small RNA-seq Services</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
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                         <a href="/cn/p/rna-data-analysis"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
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    <div class="small-12 medium-12 large-12 columns">
        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>RNA Data Analysis</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

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            <h6 style="height:60px">RNA Data Analysis</h6>
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			'name' => 'Bioinformatics Data Mining Service',
			'description' => '<h2 class="text-center"><span class="diacol">New!</span> <br />Data mining using machine learning (AI) for unique epigenetic data insights</h2>
<center><img src="https://www.diagenode.com/img/product/data-mining-long.png" /></center>
<p></p>
<div id="paper" style="text-align: center;">
<h4><a href="https://www.youtube.com/watch?v=KXjnSHz3Jk8">Watch the webinar to gain insights on how data mining can be applied for epigenetics applications</a></h4>
</div>
<center><iframe width="560" height="315" src="https://www.youtube.com/embed/KXjnSHz3Jk8" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen="allowfullscreen"></iframe></center>
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<div class="small-12 medium-12 large-12 columns">
<blockquote style="padding-bottom: 0;"><span class="label-green" style="margin-bottom: 16px; margin-left: -22px; font-size: 22px;">WHITE PAPERS</span>
<div id="portal" class="main-portal">
<div class="portal-inner"><nav class="portal-nav" style="text-align: left;">
<ul data-tab="" class="tips-menu">
<li><a href="#panel1" class="tips portal button">Smokers vs non-smokers </a></li>
<li><a href="#panel2" class="tips portal button">Breast cancer</a></li>
</ul>
</nav></div>
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<div class="tabs-content">
<div class="content active" id="panel1">
<h3 style="margin-top: 0;">Powerful new insights with epigenetic data mining.<br /> A study to distinguish smokers from non-smokers using just one droplet of blood</h3>
<p>Next generation sequencing in combination with sophisticated bioinformatics technologies for genomic, transcriptomic and epigeneomic analyses have enormous potential to establish new biomarkers for disease diagnostics, enabling true precision medicine. Analyses of liquid biopsies to measure thousands of different data points simultaneously in easily accessible body fluids (e.g. blood, urine, and saliva) are extremely promising for such biomarker studies.<br />(...)</p>
<a href="https://www.diagenode.com/en/pages/form-bioinfo" class="alert small button" target="_blank">Read more</a></div>
<div class="content" id="panel2">
<h3 style="margin-top: 0;">Data mining on DNA methylation data in cancer samples<br />Distinguishing normal from breast cancer tissue</h3>
<p>Breast cancer is the most commonly occurring cancer in women and the second most common cancer overall.</p>
<p>One important aspect of cancer tissues it that they differ from normal tissues in their epigenetic make up, especially in the DNA methylation pattern. In normal cells methylation assures the proper regulation of gene expression and stable gene silencing. DNA methylation is associated with histone modifications, and the interplay of these epigenetic modifications is crucial to regulate the functioning of the genome by changing chromatin architecture.<br />(...)</p>
<a href="https://www.diagenode.com/en/pages/form-bioinfo?app_note=23" class="alert small button" target="_blank">Read more</a></div>
</div>
</blockquote>
</div>
</div>
<p>Diagenode's new data mining service utilizes methods at the frontier of machine learning, statistics, and database systems. This enhanced service supports new discoveries that were previously not possible by analyzing patterns in large data sets to give informative new insights.</p>
<p>If you have data from patient cohorts, single cell analyses or any other heterogeneous scenarios, our service team provides enhanced support with optimal data analysis using our latest data mining capabilities. Specifically, our team applies machine learning technologies to find previously undiscovered or unobvious relationships within and across datasets. This advanced technology allows discovery of informative features from mass data, essentially &ldquo;finding a needle in a haystack.&rdquo;</p>
<p>Diagenode utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data. Our goal is to build strong classifiers that separate data into two or more classes or groups depending on associated data.</p>
<p class="extra-spaced">Different and multiple -omics data classes can be mined simultaneously. Integration with phenotypic and/or clinical data is also possible. We offer data mining services for several data classes including:</p>
<table class="extra-spaced">
<tbody>
<tr>
<td><strong>Epigenetic data</strong></td>
<td><strong>Transcriptomic data</strong></td>
</tr>
<tr>
<td>
<p>DNA Methylation (RRBS, WGBS, EPIC arrays)</p>
<p>ChIP-sequencing</p>
<p>ATAC-seq</p>
</td>
<td>
<p>mRNA-sequencing</p>
<p>Small and long non coding RNA</p>
<p>Single-cell RNA-sequencing</p>
</td>
</tr>
</tbody>
</table>
<p></p>
<p><strong>Biological Interpretation</strong></p>
<p class="extra-spaced">Machine learning classifiers also mirror the underlying biological differences between classes and are used to uncover the molecular processes at work. In order to achieve this, we offer biological interpretation services and pathway mining analyses for your data.</p>
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<p><a href="https://www.diagenode.com/en/categories/Services">Read about our wetlab services</a></p>
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<p>Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of mRNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on specific poly A selection to produce Illumina compatible libraries enriched in mRNAs.</p>
<h2>High performance mRNA-seq</h2>
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<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/rna-seq-category">See our other RNA-seq Profiling Services</a></p>',
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<h4 class="text-center"><strong>Advanced bioinformatic analysis</strong><br /> (optional for additional fee)</h4>
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<li>Demultiplexing, read quality control, trimming and filtering</li>
<li>Alignment to reference genome</li>
<li>Quantification of known transcripts</li>
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<br />
<p><em>Provided files:</em></p>
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<li>Report with sequencing statistics</li>
<li>Raw data in FASTQ format</li>
<li>FastQC report</li>
<li>Alignment in BAM format</li>
<li>Un-normalized read counts</li>
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<td style="vertical-align: top;">
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<li>Differential expression analysis</li>
<li>Gene ontology terms analysis</li>
<li>Pathway analysis</li>
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<li>Novel transcript identification</li>
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			'description' => '<p><strong>Small RNA-seq</strong> is the method of choice to investigate the regulatory network of small non-coding RNAs (&lt;200 nt length), including but not limited to miRNA transcripts. It utilizes the proprietary technology D-Plex which ensures realistic representation of diverse small non-coding RNA transcripts with minimized bias. This approach is optimal for finding biomarkers from known transcripts and discover new RNA features.</p>
<p>Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of small RNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on a template-switching technology to produce Illumina compatible libraries enriched with the large variety of small non-coding RNAs.</p>
<h2>High transcript diversity with small RNA-seq</h2>
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<li><strong>Project size</strong>: no minimum</li>
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<li><strong>Data analysis</strong> (optional for additional fee)</li>
<ul>
<li>Standard bioinformatic analysis</li>
<li>Advanced bioinformatic analysis</li>
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<li>Quantification of small RNA biotypes with specialized quantification tool MGCount (<a href="https://doi.org/10.1186/s12859-021-04544-3">software developed by Diagenode</a>)</li>
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<br />
<p><em>Provided files:</em></p>
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<li>Report with sequencing statistics</li>
<li>Raw data in FASTQ format</li>
<li>FastQC report</li>
<li>Alignment in BAM format</li>
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<h2>What do we provide with the analysis?</h2>
<p>This analysis provides information for either genes or isoforms with their expression levels.</p>
<h3 class="diacol" style="font-weight: 100;">Standard Analysis</h3>
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<h3 class="diacol" style="font-weight: 100;">Advanced Analysis</h3>
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<li>Functional gene annotation</li>
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<li>Pathway enrichment analysis on DEGs (KEGG or DOSE for human samples)</li>
<li>Alternative splicing analysis</li>
<li>Gene fusion analysis</li>
<li>Novel transcript identification</li>
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<p class="text-left">If you require a type of analysis that is not in the previous list, <a href="#" data-reveal-id="quoteModal-3062">please consult with our expert bioinformatics team</a>.</p>
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<div><center><img src="https://www.diagenode.com/img/product/services/RNA-theorie.png" /></center></div>
<p><a href="https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-021-04544-3">MGcount</a>: Diagenode has developed a bioinformatics software for counting whole-transcriptome RNA-seq reads from one or more input alignment files. It is specially designed to incorporate multi-mapping and multi-overlapping reads in the quantification using a flexible methodology that is compatible with any biotype. At the end of its execution, it produces a count matrix, compatible with any downstream analysis.</p>
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<li><span> </span>遺伝子発現の転写後調節</li>
<li><span> </span>新規転写物および差示的に発現される小RNA</li>
<li><span> </span>腫瘍抑制遺伝子発現の調節に関与するmiRNA</li>
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<li><span> </span>癌の病因における異常なmRNA転換</li>
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<p>Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis.&nbsp;</p>
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<h3><span class="green">Chromatin</span>&nbsp;analysis</h3>
<ul>
<li>ChIP-seq analysis Histone modification</li>
<li>Promoter analysis</li>
<li>Enhancer analysis</li>
<li>ChIP-seq analysis Transcription factor&nbsp;</li>
<li>(sc)ATAC-seq Chromatin accessibility</li>
<li>ChIP-seq Cas9 off-target</li>
<li>Customized NGS service</li>
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<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/chip-seq-service">Learn more</a></p>
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<div class="small-12 medium-4 large-4 columns text-left">
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<h3><span class="darkgrey">DNA methylation</span> analysis</h3>
<ul>
<li>Reduced representation bisulfite sequencing (RRBS)</li>
<li>Whole genome bisulfite sequencing (WGBS)</li>
<li>Targeted methylation</li>
<li>MeDIP-seq/hMeDIP-seq</li>
<li>Infinium MethylationEPIC array&nbsp;</li>
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<p></p>
<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/dna-methylation-profiling-services">Learn more</a></p>
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<div class="small-12 medium-4 large-4 columns text-left">
<div class="panel" style="border-color: #b11e33;">
<h3><span class="diacol"><g class="gr_ gr_44 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="44" data-gr-id="44">RNA-seq</g></span> analysis</h3>
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<li>mRNA<span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;"> analysis</span></li>
<li>Small non-coding RNA analysis</li>
<li><b></b>Whole transcriptome analysis<br /><br /><br /><br /><br /></li>
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<p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/rna-seq-category">Learn more</a></p>
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<h2 style="text-align: center;"><a class="details radius small button" href="https://www.diagenode.com/en/documents/services-flyer">DOWNLOAD OUR FLYER</a></h2>
<h2>Why Diagenode</h2>
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<li><strong>Expertise and trust</strong>: Recognized epigenetics leader, official partner of <a href="http://www.blueprint-epigenome.eu/"><g class="gr_ gr_45 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="45" data-gr-id="45">BLUEPRINT</g></a>, <a href="http://ihec-epigenomes.org/">IHEC</a> <g class="gr_ gr_46 gr-alert gr_gramm gr_inline_cards gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="46" data-gr-id="46">and</g> <a href="https://www.faang.org/">FAANG</a></li>
<li><strong>Innovative technology</strong>: Utilization of the signature Bioruptor<sup>&reg;</sup> sonication device for optimal chromatin and DNA shearing and the IP-Star<sup>&reg;</sup> Automation device give reproducible and reliable optimization and results</li>
<li><strong>Quality</strong>: Multiple QC steps in all workflows and validated antibodies plus reagents deliver superior data</li>
<li><strong>Flexibility</strong>: Extensive range of sample species and sample origins</li>
<li><strong>Experience</strong> in epigenomics profiling for a wide range of applications and fields of interest</li>
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<div class="block-distri"><span class="label-green" style="margin-bottom: 16px; margin-left: -3px;">HUMAN</span><center><img width="260" height="259" alt="services chip-seq - human" src="https://www.diagenode.com/img/categories/services/services-humans.png" caption="false" /></center></div>
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<div class="block-distri"><span class="label-green" style="margin-bottom: 16px; margin-left: -3px;">ANIMALS</span><center><img width="260" height="259" alt="services chip-seq - animals" src="https://www.diagenode.com/img/categories/services/services-animals.png" caption="false" /></center></div>
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<li>
<div class="block-distri"><span class="label-green" style="margin-bottom: 16px; margin-left: -3px;">PLANTS</span><center><img width="260" height="259" alt="services methylation-plant" src="https://www.diagenode.com/img/categories/services/services-plants.png" caption="false" /></center></div>
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<h2>12 years of expertise and trust in epigenetics</h2>
<ul>
<li><strong>End-to-end</strong> epigenetic service</li>
<li><strong>Collaborative</strong> and customized project design</li>
<li>Dedicated <strong>in-house expert</strong> for your project</li>
<li><strong>Integrative</strong> data analysis</li>
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Our Epigenomics Profiling Services helps you to complete your transcriptomic profiling analysis using next-generation sequencing (NGS). Our experienced scientists will help you design your RNA-seq experiments based on the ultimate goal of your transcriptomic profiling analysis by generating meaningful RNA-seq libraries and delivering high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</div>
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			'name' => 'Long-term effects of myo-inositol on traumatic brain injury: Epigenomic and transcriptomic studies',
			'authors' => 'Oganezovi N. et al.',
			'description' => '<h6>Background and purpose</h6>
<div class="section-paragraph">Traumatic brain injury (TBI) and its consequences remain great challenges for neurology. Consequences of TBI are associated with various alterations in the brain but little is known about long-term changes of epigenetic DNA methylation patterns. Moreover, nothing is known about potential treatments that can alter these epigenetic changes in beneficial ways. Therefore, we have examined myo-inositol (MI), which has positive effects on several pathological conditions.</div>
<h6></h6>
<h6>Methods</h6>
<div class="section-paragraph">TBI was induced in mice by controlled cortical impact (CCI). One group of CCI animals received saline injections for two months (TBI+SAL), another CCI group received MI treatment (TBI+MI) for the same period and one group served as a sham-operated control. Mice were sacrificed 4 months after CCI and changes in DNA methylome and transcriptomes were examined.</div>
<h6></h6>
<h6>Results</h6>
<div class="section-paragraph">For the first time we: (i) provide comprehensive map of long-term DNA methylation changes after CCI in the hippocampus; (ii) identify differences by methylation sites between the groups; (iii) characterize transcriptome changes; (iv) provide association between DNA methylation sites and gene expression. MI treatment is linked with upregulation of genes covering 33 biological processes, involved in immune response and inflammation. In support of these findings, we have shown that expression of BATF2, a transcription factor involved in immune-regulatory networks, is upregulated in the hippocampus of the TBI+MI group where the BATF2 gene is demethylated.</div>
<h6></h6>
<h6>Conclusion</h6>
<div class="section-paragraph">TBI is followed by long-term epigenetic and transcriptomic changes in hippocampus. MI treatment has a significant effect on these processes by modulation of immune response and biological pathways of inflammation.</div>',
			'date' => '2024-01-30',
			'pmid' => 'https://www.ibroneuroreports.org/article/S2667-2421(24)00013-7/fulltext',
			'doi' => 'https://doi.org/10.1016/j.ibneur.2024.01.009',
			'modified' => '2024-03-28 11:30:49',
			'created' => '2024-02-22 12:28:44',
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			'description' => '<p><span>Inflammatory stresses underlie endothelial dysfunction and contribute to the development of chronic cardiovascular disorders such as atherosclerosis and vascular fibrosis. The initial transcriptional response of endothelial cells to pro-inflammatory cytokines such as TNF-alpha is well established. However, very few studies uncover the effects of inflammatory stresses on chromatin architecture. We used integrative analysis of ATAC-seq and RNA-seq data to investigate chromatin alterations in human endothelial cells in response to TNF-alpha and febrile-range heat stress exposure. Multi-omics data analysis suggests a correlation between the transcription of stress-related genes and endothelial dysfunction drivers with chromatin regions exhibiting differential accessibility. Moreover, microscopy identified the dynamics in the nuclear organization, specifically, the changes in a subset of heterochromatic nucleoli-associated chromatin domains, the centromeres. Upon inflammatory stress exposure, the centromeres decreased association with nucleoli in a p38-dependent manner and increased the number of transcripts from pericentromeric regions. Overall, we provide two lines of evidence that suggest chromatin alterations in vascular endothelial cells during inflammatory stresses.</span></p>',
			'date' => '2023-10-16',
			'pmid' => 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614786/',
			'doi' => '10.1101/2023.10.11.561959',
			'modified' => '2024-03-27 15:15:21',
			'created' => '2024-03-27 15:15:21',
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            <h6 style="height:60px">Bioinformatics Data Mining Service</h6>
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            <span class="prefix">Sample species</span>
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            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">mRNA-seq Service</h6>
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</li>
<li>
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                         <a href="/cn/p/small-rna-seq-services"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



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        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Small RNA-seq Services</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

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<a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: QUOTE MODAL --><a href="#" id="small-rna-seq-services" data-reveal-id="quoteModal-3089" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Small RNA-seq Services</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/cn/p/rna-data-analysis"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



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        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>RNA Data Analysis</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

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            </div>

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        <div class="small-12 columns" >
            <h6 style="height:60px">RNA Data Analysis</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '3062',
	'antibody_id' => null,
	'name' => 'RNA Data Analysis',
	'description' => '<p>Total RNA sequencing (RNA-Seq) detects both coding and noncoding RNAs and is typically used to measure gene and transcript abundance as well as to identify novel components of the transcriptome. Messenger RNA-Seq focuses on the quantification of gene expression, the identification of unknown transcripts, the discovery of alternative splicing and gene fusion events. And finally, small non-coding RNA sequencing (sncRNA-Seq) will detect small (&lt;100 nucleotides long) RNAs that operate as key regulators in cellular processes.</p>
<div class="extra-spaced">
<h2>What do we provide with the analysis?</h2>
<p>This analysis provides information for either genes or isoforms with their expression levels.</p>
<h3 class="diacol" style="font-weight: 100;">Standard Analysis</h3>
<ul style="list-style-type: circle;">
<li>Summary statistics (total sequenced reads, total mapping reads, uniquely aligned reads, PCR duplicates, number of genes detected, average read density per gene, number of highly expressed genes, etc.)</li>
<li>Trimmed and filtered reads in fastQ files after sequencing QC</li>
<li>BAM sorted files from alignment to reference genome or transcriptome (indexed bam files and bigwig files included)</li>
<li>Matrix with expression abundance obtained with specialized quantification tool MGCount (<a href="https://doi.org/10.1186/s12859-021-04544-3">software developed by Diagenode</a>). A table of MG communities linking each original feature in the GTF file with the resultant count matrix and metadata feature identifiers.</li>
</ul>
<h3 class="diacol" style="font-weight: 100;">Advanced Analysis</h3>
<ul style="list-style-type: circle;">
<li>Comparative analysis (also called differential analysis) aimed at finding differentially expressed genes (DEGs) between two groups of samples</li>
<li>Functional gene annotation</li>
<li>Gene ontology enrichment analysis on DEGs</li>
<li>Pathway enrichment analysis on DEGs (KEGG or DOSE for human samples)</li>
<li>Alternative splicing analysis</li>
<li>Gene fusion analysis</li>
<li>Novel transcript identification</li>
</ul>
<h3 class="diacol" style="font-weight: 100;">Customized Analysis</h3>
<p class="text-left">If you require a type of analysis that is not in the previous list, <a href="#" data-reveal-id="quoteModal-3062">please consult with our expert bioinformatics team</a>.</p>
</div>
<div><center><img src="https://www.diagenode.com/img/product/services/RNA-theorie.png" /></center></div>
<p><a href="https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-021-04544-3">MGcount</a>: Diagenode has developed a bioinformatics software for counting whole-transcriptome RNA-seq reads from one or more input alignment files. It is specially designed to incorporate multi-mapping and multi-overlapping reads in the quantification using a flexible methodology that is compatible with any biotype. At the end of its execution, it produces a count matrix, compatible with any downstream analysis.</p>
<center><img src="https://www.diagenode.com/img/product/services/mGcount-img.png" width="600px" /></center>',
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