Diagenode

RNA Data Analysis

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G02030005

Total RNA sequencing (RNA-Seq) detects both coding and noncoding RNAs and is typically used to measure gene and transcript abundance as well as to identify novel components of the transcriptome. Messenger RNA-Seq focuses on the quantification of gene expression, the identification of unknown transcripts, the discovery of alternative splicing and gene fusion events. And finally, small non-coding RNA sequencing (sncRNA-Seq) will detect small (<100 nucleotides long) RNAs that operate as key regulators in cellular processes.

What do we provide with the analysis?

This analysis provides information for either genes or isoforms with their expression levels.

Standard Analysis

  • Summary statistics (total sequenced reads, total mapping reads, uniquely aligned reads, PCR duplicates, number of genes detected, average read density per gene, number of highly expressed genes, etc.)
  • Trimmed and filtered reads in fastQ files after sequencing QC
  • BAM sorted files from alignment to reference genome or transcriptome (indexed bam files and bigwig files included)
  • Matrix with expression abundance estimation (gene names in rows and sample names in columns)

Advanced Analysis

  • Comparative analysis (also called differential analysis) aimed at finding differentially expressed genes (DEGs) between two groups of samples
  • Functional gene annotation
  • Gene ontology enrichment analysis on DEGs
  • Pathway enrichment analysis on DEGs (KEGG or DOSE for human samples)
  • Alternative splicing analysis
  • Gene fusion analysis
  • Novel transcript identification

Customized Analysis

If you require a type of analysis that is not in the previous list, please consult with our expert bioinformatics team.

活动

  • Virtual ChIP workshop - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Virtual DNA Methylation - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Lausanne Genomics Days 2022
    Lausanne, Switzerland
    Feb 14-Feb 15, 2022
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