New D-Plex technology utilizes the innovative capture and amplification by tailing and switching combined with UMIs, a unique ligation-free method to produce DNA libraries for NGS from low input amounts of RNA. This solution allows you to go down to 10 pg enriched small RNA or 100 pg total RNA! There is no comparable solution on the market.
With only 30 minutes hands-on time, the optimized one-tube protocol allows your libraries to be ready in less than 5 hours!
Due to the power of template-switch technology, D-Plex Small RNA-seq Kit ensures a realistic representation of diverse small RNA species (such as miRNA, piRNA, tRNA, and siRNA) avoiding a biased miRNA enrichment as with ligation methods. It is time to finally discover RNA diversity in your sample.
D-Plex Small RNA-seq Kit has been extensively validated using different types of samples from different species (such as Arabidopsis, Paramecium, C. elegans, Mouse and Human). This figure gives gives some examples of the small RNA diversity you can achieve with D-Plex. The Kit was successfully validated on human biofluids (plasma and serum).
D-Plex Small RNA-seq Kit integrates Unique Molecular Identifiers (UMIs) in the DNA construct. While small RNA sequencing is known to suffer from ligation bias, D-Plex Small RNA-seq Kit eliminates this issue by combining template-switching with UMIs. The use of such molecular indexing technology is also an important tool for identifying and correcting PCR duplicates during NGS data analysis.
The right figure shows the important effect of UMI deduplication on small non-coding RNAs set detected at TPM >=2 in rat plasma for two different inputs: 1ng and 10pg small enriched RNA. The corrective effect is more pronounced as the input goes lower for the library preparation.
“We work with extremely limited quantities of small RNAs. In our hands, D-Plex had the highest yield compared to many other approaches. Inclusion of UMIs is essential for our small RNA analyses.”