High-throughput 3’mRNA-seq is an attractive solution to perform comparative gene expression analysis on a large number of samples. It utilizes the proprietary technology Mercurius from Alithea Genomics which enables reliable gene expression quantification for large investigational studies. This approach is optimal for assessing multiple conditions of interest or increasing statistical power underlying any differential expression analysis with larger sets of replicates.
Our Epigenomics Profiling Services assure a fully integrated service-workflow for generation of 3’mRNA-seq libraries, multiplexed sequencing and data analysis. Serviced library preparation is based on Mercurius protocol which uses early sample multiplexing to produce Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
Scalable and affordable 3’mRNA-seq
Reliable gene expression quantification
Accurate comparison of gene expression levels
Leveraging the innovative Mercurius technology
Handling large number of samples simultaneously
Working with very low-quality RNA samples
Fast turnaround time (from 4 to 6 weeks)
Innovative approach using early sample multiplexing
BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing Daniel Alpern, Vincent Gardeux, Julie Russeil, Bastien Mangeat, Antonio C. A. Meireles-Filho, Romane Breysse, David Hacker and Bart Deplancke Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq ha...