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Epigenomics Profiling Services

DNA Methylation Profiling Services

DNA-methylation -Diagenode

The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.

RRBS-Diagenode

Infinium MethylationEPIC Array Service

The Infinium MethylationEpic Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allows to quantitatively detect the total methylation level of over 850,000 CpG positions throughout the human genome at single nucleotide resolution.   

  • Cost-effective with rapid turnaround time
  • Over 850,000 CpGs detected in human samples at single nucleotide resolution
  • Quantitative interrogation of CpG, non-CpG, and CHH sites
  • Differentially methylated site analysis
  • Available for 5hmC profiling

Reduced representation bisulfite sequencing (RRBS)

RRBS provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level without the higher costs associated with whole genome bisulfite sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a cost-effective method for analyzing DNA methylation by reducing the part of the genome that actually needs to be sequenced and focusing on relevant CpG islands.

  • CpG rich region (5-mC): single nucleotide resolution
  • Epigenetic biomarker discovery
  • Methylation patterns of CpG rich regions across the genome
  • Covers up to 4M CpG sites in Human

Whole genome bisulfite sequencing (WGBS)

WGBS is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina® platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. Genome-wide bisulfite sequencing is suitable for studying DNA methylation sites and their role in gene regulation

  • Genome-wide 5-mC: single nucleotide resolution
  • Epigenetic biomarker discovery
  • Methylation patterns of CpG, CHG, and CHH regions across the genome
  • Epigenome-wide association studies (EWAS)

MeDIP/hMeDIP-seq

Targeted DNA methylation analysis


Products

Cat. No.ProductFormatPrice
G02090004 Infinium MethylationEPIC and Hydroxymethylation oxEPIC Array Service
The Infinium MethylationEPIC & Hydroxymethylation oxEPIC Array utilizes a genome-wide DNA methylation analysis technique based on selective oxidat...
G02090000 Infinium MethylationEPIC Array Service
The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allow...
G02100000 Bioinformatics Data Mining Service
New! Data mining using machine learning (AI) for unique epigenetic data insights Watch the webinar to gain insights on how data mining can be applied ...
Custom
G02020000 DNA Methylation Profiling (RRBS Service)
*Offer valid only until Agust 31, 2020 for a minimum of 10 samples. Samples must be provided in increments of 10. Alignment to reference gen...
G02050000 MeDIP-seq service (Methylated DNA IP Sequencing)
Methylated DNA immunoprecipitation sequencing (MeDIP-Seq) is used to study 5-mC genome-wide. In this assay, specific antibodies against 5-mC are used to capt...
G02070000 Targeted DNA methylation service
Targeted DNA methylation analysis allows for the quantitative study of cytosine methylation at known and precise locations throughout the genome. Depending o...
G02040000 WGBS service (Whole Genome Bisulfite Sequencing)
Whole-genome bisulfite sequencing (WGBS) is the most comprehensive next generation sequencing  for DNA methylation profiling, allowing single-base resol...

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