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WGBS Service (Whole Genome Bisulfite Sequencing)

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Whole-genome bisulfite sequencing (WGBS) is the most comprehensive next generation sequencing  for DNA methylation profiling, evaluating methylation state of nearly every CpG sites of the entire genome. By comparing untreated and bisulfite-treated sequences, the location of the methylated cytosines are determined with accuracy. Genome-wide bisulfite sequencing provides deep insights into gene regulation, allowing identification of novel epigenetic markers and targets for disease.

Highest coverage for DNA methylation studies

  • Genome-wide screening for DNA methylation at single-base resolution
  • Detection of global methylation patterns
  • High coverage for 5-mC including in low-CpG-density regions and outside of CpG islands
  • Identification of regions or even loci with differential methylation between groups using bioinformatics tools
  • Compatible with bisulfite-converted DNA samples enriched by ChIP or other methods
  • A dedicated scientist driving your project with high touch communication
  • Comprehensive end-to-end service: from wet-lab to bioinformatics

  • Description

    WGBS Service includes:

    QC of the genomic DNA

    • Measurement of DNA concentration
    • Assessment of DNA quality

    Preparation of WGBS libraries

    • gDNA shearing on Bioruptor Pico
    • Bisulfite conversion
    • Library preparation (end repair, adenylation, adaptor ligation)  
    • Library amplification
    • Size selection and clean-up
    • QC of the WGBS libraries (DNA concentration, analysis of the profile)

    Deep sequencing

    • Samples are sent for sequencing on an Illumina platform  
    • Single-read or paired-end  
    • 50bp to 150bp read length  
    • Coverage to be defined

  • Bioinformatic analysis

    Standard analysis

    Methylation calling:

    Alignment of bisulfite sequencing data to reference genome and determination of methylation status of CpG nucleotides.

    Additional analysis on request:  

    Differential methylation analysis:

    Comparison of methylation status of CpG nucleotides between sample groups.

    Annotation with genomic regions:

    Annotation of differentially methylated CpGs or of DMRs with genomic regions such as introns, exons, enhancers (when available), promoters, intergenic regions.  

    Gene ontology terms analysis:

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.  

    Pathway analysis:

    Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.  

  •  Documents
    Epigenomics Profiling Services FLYER
    Chromatin analysis DNA methylation services RNA-seq analysis
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: WGBS Service (Whole Genome Bisulfite Sequencing) (Diagenode Cat# G02040000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Development and epigenetic plasticity of murine Müller glia.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The ability to regenerate the entire retina and restore lost sight after injury is found in some species and relies mostly on the epigenetic plasticity of Müller glia. To understand the role of mammalian Müller glia as a source of progenitors for retinal regeneration, we investigated changes in gene expres...

    The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal...

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