Diagenode

WGBS Service (Whole Genome Bisulfite Sequencing)

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  •  Description
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Catalog Number
Format
G02040000

Whole-genome bisulfite sequencing (WGBS) is the method of choice for obtaining a comprehensive DNA methylation profiling, evaluating the methylation patterns of nearly every CpG site of the entire genome. By comparing untreated and bisulfite-treated sequences, the location of the methylated cytosines are determined with accuracy. Genome-wide bisulfite sequencing provides deep insights into gene regulation, allowing identification of novel epigenetic markers and targets for disease.

In-depth DNA methylation analysis

  • Very powerful solution for genome-wide biomarker discovery
  • Single-nucleotide resolution
  • Evaluation of the methylation status of nearly every CpG sites of the entire genome 
  • Detection of global methylation patterns including outside of CpG islands and in low-CpG-density regions  
  • Identification of regions or even loci with differential methylation between groups using bioinformatics tools
  • Providing a better understanding of development and disease

Comprehensive end-to-end service

  • From library preparation to data analysis
  • A dedicated scientist driving your project with high touch communication
  • High-quality bioinformatic support to assist you with data analysis

See our other DNA Methylation Profiling Services

  • Services Workflow

    WGBS Service includes:

    QC of the genomic DNA

    • Measurement of DNA concentration
    • Assessment of DNA quality

    Preparation of WGBS libraries

    • gDNA shearing on Bioruptor Pico
    • Bisulfite conversion
    • Library preparation (end repair, adenylation, adaptor ligation)  
    • Library amplification
    • Size selection and clean-up
    • QC of the WGBS libraries (DNA concentration, analysis of the profile)

    Deep sequencing

    • Samples are sent for sequencing on an Illumina platform  
    • Single-read or paired-end  
    • 50bp to 150bp read length  
    • Coverage to be defined


  • Bioinformatics Analysis

    Analysis

    Features

    Standard
    • FASTQ raw data
    • FASTQC quality control insights
    • Alignment of bisulfite sequencing data against reference genome
    • Methylation calling and extraction
    • Summary statistics
    Differential methylation analysis
    • Methylation level analysis
    • Differentially methylated CpGs (DMCs) analysis
    • Differentially methylated regions (DMRs) analysis
    • Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)
    • Clustering analysis

    Gene ontology terms analysis

    • Enrichment analysis on gene associated with DMCs and DMRs
    • Get functional insights

    Pathway analysis

    • Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)
    • Get mechanistic insights

  •  Documents
    Epigenomics Profiling Services FLYER
    Chromatin analysis DNA methylation services RNA-seq analysis
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: WGBS Service (Whole Genome Bisulfite Sequencing) (Diagenode Cat# G02040000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Development and epigenetic plasticity of murine Müller glia.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The ability to regenerate the entire retina and restore lost sight after injury is found in some species and relies mostly on the epigenetic plasticity of Müller glia. To understand the role of mammalian Müller glia as a source of progenitors for retinal regeneration, we investigated changes in gene expres...

    The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal...

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