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WGBS service (Whole Genome Bisulfite Sequencing)

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Whole-genome bisulfite sequencing (WGBS) is the most comprehensive next generation sequencing  for DNA methylation profiling, allowing single-base resolution of 5-mC within the whole genome. By comparing treated and untreated sequences, the location of the methylated cytosines are determined.

Highest coverage for DNA methylation studies

  • Genome-wide screening for DNA methylation and single-base resolution
  • High coverage for 5-mC in low density and repeat regions
  • Dedicated scientist drives each project with high touch communication

  • Comprehensive service – Bisulfite conversion, library preparation, sequencing, and analysis

  • Description

    WGBS services include:

    QC of the genomic DNA

    • Measurement of DNA concentration
    • Assessment of the DNA quality

    Preparation of WGBS libraries*

    • gDNA shearing on Bioruptor® Pico
    • Library preparation (end repair, adenylation, methylated adaptor ligation)  
    • Size selection  
    • Bisulfite conversion
    • Library amplification  QC of the WGBS libraries (DNA concentration, analysis of the profile)

    Deep sequencing

    • Samples are sent for sequencing on an Illumina platform  
    • Single-read or paired-end  
    • 50bp to 150bp read length  
    • Coverage to be defined

    *Full protocol details can be viewed in the manual from the  Premium Whole Genome Bisulfite Sequencing (WGBS) Kit

  • Bioinformatic analysis

    Standard analysis

    Methylation calling:

    Alignment of bisulfite sequencing data to reference genome and determination of methylation status of CpG nucleotides.

    Additional analysis on request:  

    Differential methylation analysis:

    Comparison of methylation status of CpG nucleotides between sample groups.

    Annotation with genomic regions:

    Annotation of differentially methylated CpGs or of DMRs with genomic regions such as introns, exons, enhancers (when available), promoters, intergenic regions.  

    Gene ontology terms analysis:

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.  

    Pathway analysis:

    Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.  

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    How to properly cite this product in your work

    Diagenode strongly recommends using this: WGBS service (Whole Genome Bisulfite Sequencing) (Diagenode Cat# G02040000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Development and epigenetic plasticity of murine Müller glia.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The ability to regenerate the entire retina and restore lost sight after injury is found in some species and relies mostly on the epigenetic plasticity of Müller glia. To understand the role of mammalian Müller glia as a source of progenitors for retinal regeneration, we investigated changes in gene expres...

    The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal...

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