Alignment of bisulfite sequencing data to reference genome and determination of methylation status of CpG nucleotides.
Additional analysis on request:
Differential methylation analysis:
Comparison of methylation status of CpG nucleotides between sample groups.
Annotation with genomic regions:
Annotation of differentially methylated CpGs or of DMRs with genomic regions such as introns, exons, enhancers (when available), promoters, intergenic regions.
Gene ontology terms analysis:
Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.
Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.