Diagenode

WGBS受託サービス (全ゲノムバイサルファイトシーケンシング)

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  •  資料
  •  関連記事
  •   お見積り
カタログ番号
フォーマット
G02040000

全ゲノムバイサルファイトシークエンシング(WGBS)は、DNAメチル化プロファイリングの中でも最も包括的な次世代シーケンシングであり、ゲノム全体で5-mCの単一塩基分解能を可能にします。処理された配列と未処理の配列とを比較することにより、メチル化されたシトシンの位置が決定されます。

DNAメチル化研究において最も高いカバレッジ

  • ゲノム全体のDNAメチル化と単一塩基分解能スクリーニング
  • 低密度と反復領域における5mCの高カバレッジ
  • 専任の科学者がハイタッチコミュニケーションで各プロジェクトをサポート
  • 包括的なサービス - バイサルファイト処理、ライブラリーの調製、シーケンシングと解析

  • 詳細

    WGBS services include:

    QC of the genomic DNA

    • Measurement of DNA concentration
    • Assessment of the DNA quality

    Preparation of WGBS libraries*

    • gDNA shearing on Bioruptor® Pico
    • Library preparation (end repair, adenylation, methylated adaptor ligation)  
    • Size selection  
    • Bisulfite conversion
    • Library amplification  QC of the WGBS libraries (DNA concentration, analysis of the profile)

    Deep sequencing

    • Samples are sent for sequencing on an Illumina platform  
    • Single-read or paired-end  
    • 50bp to 150bp read length  
    • Coverage to be defined

    *Full protocol details can be viewed in the manual from the  Premium Whole Genome Bisulfite Sequencing (WGBS) Kit

  • バイオインフォマティック解析

    Standard analysis

    Methylation calling:

    Alignment of bisulfite sequencing data to reference genome and determination of methylation status of CpG nucleotides.

    Additional analysis on request:  

    Differential methylation analysis:

    Comparison of methylation status of CpG nucleotides between sample groups.

    Annotation with genomic regions:

    Annotation of differentially methylated CpGs or of DMRs with genomic regions such as introns, exons, enhancers (when available), promoters, intergenic regions.  

    Gene ontology terms analysis:

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.  

    Pathway analysis:

    Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.  

  •  資料
    Full services brochure BROCHURE
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: WGBS受託サービス (全ゲノムバイサルファイトシーケンシング) (Diagenode Cat# G02040000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Development and epigenetic plasticity of murine Müller glia.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The ability to regenerate the entire retina and restore lost sight after injury is found in some species and relies mostly on the epigenetic plasticity of Müller glia. To understand the role of mammalian Müller glia as a source of progenitors for retinal regeneration, we investigated changes in gene expres...

    The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal...

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