Diagenode

>>>   Click for Diagenode’s approach to COVID-19

WGBS受託サービス (全ゲノムバイサルファイトシーケンシング)

default alt
  •  詳細
  •  資料
  •  関連記事
  •   お見積り
カタログ番号
フォーマット
G02040000

全ゲノムバイサルファイトシークエンシング(WGBS)は、DNAメチル化プロファイリングの中でも最も包括的な次世代シーケンシングであり、ゲノム全体で5-mCの単一塩基分解能を可能にします。処理された配列と未処理の配列とを比較することにより、メチル化されたシトシンの位置が決定されます。

DNAメチル化研究において最も高いカバレッジ

  • ゲノム全体のDNAメチル化と単一塩基分解能スクリーニング
  • 低密度と反復領域における5mCの高カバレッジ
  • 専任の科学者がハイタッチコミュニケーションで各プロジェクトをサポート
  • 包括的なサービス - バイサルファイト処理、ライブラリーの調製、シーケンシングと解析

  • 詳細

    WGBS Service includes:

    QC of the genomic DNA

    • Measurement of DNA concentration
    • Assessment of DNA quality

    Preparation of WGBS libraries

    • gDNA shearing on Bioruptor Pico
    • Bisulfite conversion
    • Library preparation (end repair, adenylation, adaptor ligation)  
    • Library amplification
    • Size selection and clean-up
    • QC of the WGBS libraries (DNA concentration, analysis of the profile)

    Deep sequencing

    • Samples are sent for sequencing on an Illumina platform  
    • Single-read or paired-end  
    • 50bp to 150bp read length  
    • Coverage to be defined


  • バイオインフォマティック解析

    Analysis

    Features

    Standard
    • FASTQ raw data
    • FASTQC quality control insights
    • Alignment of bisulfite sequencing data against reference genome
    • Methylation calling and extraction
    • Summary statistics
    Differential methylation analysis
    • Methylation level analysis
    • Differentially methylated CpGs (DMCs) analysis
    • Differentially methylated regions (DMRs) analysis
    • Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)
    • Clustering analysis

    Gene ontology terms analysis

    • Enrichment analysis on gene associated with DMCs and DMRs
    • Get functional insights

    Pathway analysis

    • Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)
    • Get mechanistic insights

  •  資料
    Epigenomics Profiling Services FLYER
    Chromatin analysis DNA methylation services RNA-seq analysis
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: WGBS受託サービス (全ゲノムバイサルファイトシーケンシング) (Diagenode Cat# G02040000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Development and epigenetic plasticity of murine Müller glia.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The ability to regenerate the entire retina and restore lost sight after injury is found in some species and relies mostly on the epigenetic plasticity of Müller glia. To understand the role of mammalian Müller glia as a source of progenitors for retinal regeneration, we investigated changes in gene expres...

    The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal...

  •  関連商品

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics