Development and epigenetic plasticity of murine Müller glia.

Dvoriantchikova G, Seemungal RJ, Ivanov D

The ability to regenerate the entire retina and restore lost sight after injury is found in some species and relies mostly on the epigenetic plasticity of Müller glia. To understand the role of mammalian Müller glia as a source of progenitors for retinal regeneration, we investigated changes in gene expression during differentiation of retinal progenitor cells (RPCs) into Müller glia. We also analyzed the global epigenetic profile of adult Müller glia. We observed significant changes in gene expression during differentiation of RPCs into Müller glia in only a small group of genes. We found a high similarity between RPCs and Müller glia on the transcriptomic and epigenomic levels. Our findings also indicate that Müller glia are epigenetically very close to late-born retinal neurons, but not early-born retinal neurons. Importantly, we found that key genes required for phototransduction were highly methylated. Thus, our data suggest that Müller glia are epigenetically very similar to late RPCs. Meanwhile, obstacles for regeneration of the entire mammalian retina from Müller glia may consist of repressive chromatin and highly methylated DNA in the promoter regions of many genes required for the development of early-born retinal neurons. In addition, DNA demethylation may be required for proper reprogramming and differentiation of Müller glia into rod photoreceptors.

IP-Star Compact
Microplex Library Preparation kit
True MicroChIP kit
Chromatin Profiling (ChIP-seq) Services

Share this article

July, 2019


Products used in this publication

  • cut and tag antibody icon
    H3K27me3 polyclonal antibody
  • cut and tag antibody icon
    H3K4me1 polyclonal antibody
  • cut and tag antibody icon
    H3K4me3 polyclonal antibody
  • cut and tag antibody icon
    H3K9me3 polyclonal antibody
  • ChIP kit icon
    True MicroChIP kit
  • ChIP kit icon
    MicroPlex Library Preparation Kit v2 (12 indices)
  • default alt
    ChIP-seq/ChIP-qPCR プロファイリングサービス
  • default alt
    WGBS (Whole Genome Bisulfite Sequencing) and EM...


  • APHL 2024
    Milwaukee, Wisconsin, USA
    May 6-May 9, 2024



The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics