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ChIP-seq/ChIP-qPCR プロファイリングサービス

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カタログ番号
フォーマット
G02010000

ChIP-seqをもっと身近に:豊富なフルサービス

  • 経験と実績:IHEC-BLUEPRINT Epigenome Consortiaの公式パートナーであり、ChIP-seqにおける10年の実績と経験

  • カスタマイズ可能なサポートプラン

  • 専門チームによるプロジェクト調整
  • ENCODE標準の高品質データ

    現在、エピゲノム研究に最も選ばれているのはクロマチン免疫沈降(ChIP)アッセイです。ChIPは、特定のタンパク質または修飾された形態のタンパク質とゲノムDNA領域間の相互作用を研究する貴重な方法です
ChIPは複雑で難しいという印象が強いですが、適切なツールがあれば確実な実験ができます。 ChIPの原理は、実は単純で特異的抗原を含むクロマチン画分の選択的濃縮と関係します。 目的のタンパク質またはタンパク質修飾を認識する抗体を使用することで、in vivoでゲノムの1つまたは複数の位置で、その抗原の相対的存在量を決定できます。

  • 詳細
    Chromatin shearing
    • Chromatin shearing using Bioruptor® technology
    • Isothermal chromatin shearing, preservation of epitopes
    • Homogeneous fragment size distribution
    • More than 3,000 Bioruptor citations in peer-reviewed publications
    Chromatin Immunoprecipitation
    • Choice of 200 ChIP & ChIP-Seq grade antibodies
    • Sample automation enables reproducibility
    • inputs from as little as 10,000 cells/IP
    • Optimized ChIP protocols maximize signal to noise ratio
    Library Preparation and Sequencing
    • Optimized library preparation for low amounts of DNA (50pg)
    • Minimal PCR amplification to reduce bias
    • Outstanding sequencing results (high coverage, low duplicates)
    • Rapid run available for shorter turnaround time
    • Completely customizable
    • Specific analyses available
  • バイオインフォマティック解析

    Analysis

    Features

    Standard Quality check, alignment to reference genome, identification of enriched regions (peak calling) is included.
    Annotation in genomic regions Annotation of ChIP-Seq peaks with genomic regions such as introns, exons, enhancers (when available), promoters, intergenic regions.
    Differential binding analysis Identification and annotation of differential binding between samples based on previously identified ChIP-seq peaks.
    Gene ontology terms analysis Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.
    Pathway analysis Identify biochemical pathways in which genes associated with differentially bound regions may be overrepresented
    Visualization of specific genomic regions Visualization of results (i.e. sequencing data, peaks) at specific genomic regions (e.g. genes, promoters) in publication-ready images (human, mouse, rat)
  •  資料
    Epigenomics Profiling Services FLYER
    Chromatin analysis DNA methylation services RNA-seq analysis
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ChIP-seq/ChIP-qPCR プロファイリングサービス (Diagenode Cat# G02010000). Click here to copy to clipboard.

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    Development and epigenetic plasticity of murine Müller glia.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The ability to regenerate the entire retina and restore lost sight after injury is found in some species and relies mostly on the epigenetic plasticity of Müller glia. To understand the role of mammalian Müller glia as a source of progenitors for retinal regeneration, we investigated changes in gene expres...

    The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.
    Dvoriantchikova G, Seemungal RJ, Ivanov D
    The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal...

    Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells.
    Martine Uittenbogaard, Christine A. Brantner, Anne Chiaramello1
    During neural development, epigenetic modulation of chromatin acetylation is part of a dynamic, sequential and critical process to steer the fate of multipotent neural progenitors toward a specific lineage. Pan-HDAC inhibitors (HDCis) trigger neuronal differentiation by generating an "acetylation" signature and prom...

    The epigenetic architecture at gene promoters determines cell type-specific LPS tolerance
    Kerstin Klein , Mojca Frank-Bertoncelj , Emmanuel Karouzakis , Renate E. Gay , Christoph Kolling , Adrian Ciurea , Nagihan Bostanci , Georgios N. Belibasakis , Lih-Ling Lin , Oliver Distler , Steffen Gay , Caroline Ospelt
    Synovial fibroblasts (SF) drive inflammation and joint destruction in chronic arthritis. Here we show that SF possess a distinct type of LPS tolerance compared to macrophages and other types of fibroblasts. In SF and dermal fibroblasts, genes that were non-tolerizable after repeated LPS stimulation included proinfla...

    PPARγ Links BMP2 and TGFβ1 Pathways in Vascular Smooth Muscle Cells, Regulating Cell Proliferation and Glucose Metabolism
    Laurent Calvier, Philippe Chouvarine, Ekaterina Legchenko, Nadine Hoffmann, Jonas Geldner, Paul Borchert, Danny Jonigk, Miklos M. Mozes, Georg Hansmann
    BMP2 and TGFβ1 are functional antagonists of pathological remodeling in the arteries, heart, and lung; however, the mechanisms in VSMCs, and their disturbance in pulmonary arterial hypertension (PAH), are unclear. We found a pro-proliferative TGFβ1-Stat3-FoxO1 axis in VSMCs, and PPARγ as in...

    Epigenetically-driven anatomical diversity of synovial fibroblasts guides joint-specific fibroblast functions
    Mojca Frank-Bertoncelj, Michelle Trenkmann, Kerstin Klein, Emmanuel Karouzakis, Hubert Rehrauer, Anna Bratus, Christoph Kolling, Maria Armaka, Andrew Filer, Beat Michel, Renate E. Gay, Christopher D. Buckley, George Kollias, Steffen Gay & Caroline Ospelt
    A number of human diseases, such as arthritis and atherosclerosis, include characteristic pathology in specific anatomical locations. Here we show transcriptomic differences in synovial fibroblasts from different joint locations and that HOX gene signatures reflect the joint-specific origins of mouse and human synov...

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