Diagenode

ChIP-seq/ChIP-qPCR Profiling service

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Catalog Number
Format
G02010000

What we give to you - ChIP-sequencing and ChIP-qPCR analysis

  • Integrative or individual analyses for chromatin with ENCODE standards
  • End-to-end wet lab service and full ChIP-seq analysis
  • Collaborative and customized project design to meet your needs
  • Dedicated in-house expert
  • Integrative epigenomics data analysis and presentation-ready graphs
  • Expert, extensive bioinformatics

  • Description
    Chromatin shearing
    • Chromatin shearing using Bioruptor® technology
    • Isothermal chromatin shearing, preservation of epitopes
    • Homogeneous fragment size distribution
    • More than 3,000 Bioruptor citations in peer-reviewed publications
    Chromatin Immunoprecipitation
    • Choice of 200 ChIP & ChIP-Seq grade antibodies
    • Sample automation enables reproducibility
    • inputs from as little as 10,000 cells/IP
    • Optimized ChIP protocols maximize signal to noise ratio
    Library Preparation and Sequencing
    • Optimized library preparation for low amounts of DNA (50pg)
    • Minimal PCR amplification to reduce bias
    • Outstanding sequencing results (high coverage, low duplicates)
    • Rapid run available for shorter turnaround time
  • Bioinformatic analysis

    Analysis

    Features

    Standard Quality check, alignment to reference genome, identification of enriched regions (peak calling) is included.
    Annotation in genomic regions Annotation of ChIP-Seq peaks with genomic regions such as introns, exons, enhancers (when available), promoters, intergenic regions.
    Differential binding analysis Identification and annotation of differential binding between samples based on previously identified ChIP-seq peaks.
    Gene ontology terms analysis Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.
    Pathway analysis Identify biochemical pathways in which genes associated with differentially bound regions may be overrepresented
    Visualization of specific genomic regions Visualization of results (i.e. sequencing data, peaks) at specific genomic regions (e.g. genes, promoters) in publication-ready images (human, mouse, rat)
  • Applications
    Histone ChIP-seq services
    Use our services to explore ChIP-seq histone modifications down to 10,000 cellsPost-translational modification of histones regulates gene expression. With our Epigenomic Profiling Services, study regulatory elements and their interacting proteins ... Read more
    Transcription factor ChIP-seq services
    Explore the effects of transcription factor binding through ChIP-seq analysis of a multitude of TFs including:CTCF: transcriptional repressor and insulator activityp300/CBP: histone acetyltransferasePol II, p53, and moreEpigenetic writers, readers... Read more
    Custom NGS services
    Low input libraries from picogramsSpecific analysesComplete customized projects Read more
  • Documents
    The Diagenode Epigenetics custom service POSTER
    Complete workflows for genome-scale DNA methylation and histone marks analysis Epigenetics is cr...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ChIP-seq/ChIP-qPCR Profiling service (Diagenode Cat# G02010000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The epigenetic architecture at gene promoters determines cell type-specific LPS tolerance
    Kerstin Klein , Mojca Frank-Bertoncelj , Emmanuel Karouzakis , Renate E. Gay , Christoph Kolling , Adrian Ciurea , Nagihan Bostanci , Georgios N. Belibasakis , Lih-Ling Lin , Oliver Distler , Steffen Gay , Caroline Ospelt
    Synovial fibroblasts (SF) drive inflammation and joint destruction in chronic arthritis. Here we show that SF possess a distinct type of LPS tolerance compared to macrophages and other types of fibroblasts. In SF and dermal fibroblasts, genes that were non-tolerizable after repeated LPS stimulation included proinfla...

    PPARγ Links BMP2 and TGFβ1 Pathways in Vascular Smooth Muscle Cells, Regulating Cell Proliferation and Glucose Metabolism
    Laurent Calvier, Philippe Chouvarine, Ekaterina Legchenko, Nadine Hoffmann, Jonas Geldner, Paul Borchert, Danny Jonigk, Miklos M. Mozes, Georg Hansmann
    BMP2 and TGFβ1 are functional antagonists of pathological remodeling in the arteries, heart, and lung; however, the mechanisms in VSMCs, and their disturbance in pulmonary arterial hypertension (PAH), are unclear. We found a pro-proliferative TGFβ1-Stat3-FoxO1 axis in VSMCs, and PPARγ as in...

    Epigenetically-driven anatomical diversity of synovial fibroblasts guides joint-specific fibroblast functions
    Mojca Frank-Bertoncelj, Michelle Trenkmann, Kerstin Klein, Emmanuel Karouzakis, Hubert Rehrauer, Anna Bratus, Christoph Kolling, Maria Armaka, Andrew Filer, Beat Michel, Renate E. Gay, Christopher D. Buckley, George Kollias, Steffen Gay & Caroline Ospelt
    A number of human diseases, such as arthritis and atherosclerosis, include characteristic pathology in specific anatomical locations. Here we show transcriptomic differences in synovial fibroblasts from different joint locations and that HOX gene signatures reflect the joint-specific origins of mouse and human synov...

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