Diagenode

>>>   Click for Diagenode’s approach to COVID-19

Multi-omic analysis of gametogenesis reveals a novel signature at the promoters and distal enhancers of active genes.


Crespo M, Damont A, Blanco M, Lastrucci E, Kennani SE, Ialy-Radio C, Khattabi LE, Terrier S, Louwagie M, Kieffer-Jaquinod S, Hesse AM, Bruley C, Chantalat S, Govin J, Fenaille F, Battail C, Cocquet J, Pflieger D

Epigenetic regulation of gene expression is tightly controlled by the dynamic modification of histones by chemical groups, the diversity of which has largely expanded over the past decade with the discovery of lysine acylations, catalyzed from acyl-coenzymes A. We investigated the dynamics of lysine acetylation and crotonylation on histones H3 and H4 during mouse spermatogenesis. Lysine crotonylation appeared to be of significant abundance compared to acetylation, particularly on Lys27 of histone H3 (H3K27cr) that accumulates in sperm in a cleaved form of H3. We identified the genomic localization of H3K27cr and studied its effects on transcription compared to the classical active mark H3K27ac at promoters and distal enhancers. The presence of both marks was strongly associated with highest gene expression. Assessment of their co-localization with transcription regulators (SLY, SOX30) and chromatin-binding proteins (BRD4, BRDT, BORIS and CTCF) indicated systematic highest binding when both active marks were present and different selective binding when present alone at chromatin. H3K27cr and H3K27ac finally mark the building of some sperm super-enhancers. This integrated analysis of omics data provides an unprecedented level of understanding of gene expression regulation by H3K27cr in comparison to H3K27ac, and reveals both synergistic and specific actions of each histone modification.

Tags
IP-Star Compact
Microplex Library Preparation kit
iDeal ChIP-seq Kit for Histones
Chromatin Profiling (ChIP-seq) Services

Share this article

Published
March, 2020

Source

Products used in this publication

  • ChIP kit icon
    C01010051
    iDeal ChIP-seq kit for Histones
  • ChIP kit icon
    C05010012
    MicroPlex Library Preparation Kit v2 (12 indices)
  • ChIP kit icon
    C05010014
    MicroPlex Library Preparation Kit v2 (48 indices)
  • some alt
    B03000002
    SX-8G IP-Star® Compact Automated System
  • default alt
    G02010000
    ChIP-seq/ChIP-qPCR プロファイリングサービス

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics