Human Methylome Service

Human Methylome is our NGS service for comprehensive DNA methylation profiling from low-input liquid biopsy samples, as well as PBMCs, solid tissues, and cell lines. The service uses an off-the-shelf panel to analyze more than 3.98 million CpG sites. By capturing these CpGs on both strands, we generate more than 8 million CpG measurements with high coverage ensuring accurate DNA methylation detection.

This comprehensive approach can be used for biomarker discovery as an alternative to gold-standard, genome-wide DNA methylation methods such as WGBS or EM-seq. It offers the advantage of higher coverage across a predefined set of CpGs in a cost-effective manner.

The Cost-Effective Alternative for Methylation Biomarker Discovery in Low-Input cfDNA

• Highly robust and reproducible across different operators, replicates, and input amounts
• Broad sample compatibility, from PBMCs to cell lines, and from solid tissues to liquid biopsy samples, including plasma and seminal fluid
• Suitable for low-input liquid biopsy, down to 5 ng of cfDNA
• End-to-end service, from cfDNA extraction to bioinformatic analysis
• Multi-omics integration with cfRNA (D-Plex cell-free RNA-seq) and histone PTMs from circulating nucleosomes (Nu.Q Discover)