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NEW! Human Methylome enables high coverage genome-wide DNA methylation analysis at the single nucleotide level in Human samples and it is ideal for biomarker discovery. The assay benefits from the advantages of Twist hybrid-capture panel that targets only 83Mb genomic regions containing important DNA methylation site thus enabling a very high CpG coverage. The technology is cost-efficient since it avoids the sequencing of the whole 3.2Gb human genome which includes regions without CpGs. By using Enzymatic Methylation (EM-seq) DNA is converted without further degradation and fragmentation leading to reduced bias and homogenous targeted capture.
How to properly cite this product in your workDiagenode strongly recommends using this: Human Methylome Service (Diagenode Cat# G02180000). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'services' ), 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'Human Methylome Service | Diagenode', 'product' => array( 'Product' => array( 'id' => '3218', 'antibody_id' => null, 'name' => 'Human Methylome Service', 'description' => '<p>NEW! <strong>Human Methylome</strong> enables high coverage genome-wide DNA methylation analysis at the single nucleotide level in Human samples and it is ideal for biomarker discovery. The assay benefits from the advantages of Twist hybrid-capture panel that targets only 83Mb genomic regions containing important DNA methylation site thus enabling a very high CpG coverage. The technology is cost-efficient since it avoids the sequencing of the whole 3.2Gb human genome which includes regions without CpGs. By using Enzymatic Methylation (EM-seq) DNA is converted without further degradation and fragmentation leading to reduced bias and homogenous targeted capture.</p><h2>High Coverage Genome-wide DNA methylation analysis</h2><ul class="square"><li>Single nucleotide resolution with enzymatic methylation (EM-seq)</li><li>Compatible with low input and highly fragmented cfDNA and FFPE</li><li>DNA inputs ranging from 200ng down to 10 ng</li><li>Robust (Hybrid-Capture) and cost-effective (reduced sequencing) solution</li><li>High coverage of 200X on CpG containing-region</li><li>Up to 18 million CpGs detected and 9 millions covered more than 10X</li><li>Covers more than 90% of the CpGs included in the Infinium EPIC array</li><li>Suitable for epigenetic biomarker discovery</li></ul><h2>Complete end-to-end service</h2><ul class="square"><li>From DNA QC to raw sequencing data</li><li>High-quality <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatic support</a> (on request) to assist you with data analysis</li><li>Leveraging</li><li>Applicable to difficult samples such as cfDNA and FFPE</li><li>High CpG average coverage thanks to enzymatic conversion and hybrid-capture</li></ul>', 'label1' => '', 'info1' => '<p>The Human Methylome Panel enables the identification and study of methylation biomarkers spanning a wide range of targets and applications. The 123 Mb panel provides a targeted NGS-based DNA methylation assay, covering more than 84% of human CpG island sites, which is 4 times the number of CpGs than other technologies and much more cost effective than WGBS. The high capture efficiency increases the sensitivity of detection and achieves a depth of coverage of 90% of bases at 30x coverage with high probe specificities.</p> <p>- Optimal service for biomarker discovery in cancer, neurodegenerative, cardiovascular and metabolic diseases<br /> - Ideal for challenging clinical samples such as liquid biopsies (plasma cfDNA) or FFPE</p> <p></p> <h2>Workflow</h2> <p><img src="https://www.diagenode.com/img/methylome-workflow.png" /></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => '', 'info2' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '', 'catalog_number' => 'G02180000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => 'on request', 'price_USD' => 'on request', 'price_GBP' => 'on request', 'price_JPY' => 'on request', 'price_CNY' => 'on request', 'price_AUD' => 'on request', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'human-methylome-service', 'meta_title' => 'Human Methylome Service | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-14 11:24:59', 'created' => '2022-04-28 15:53:29', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array(), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $carrousel_order = array( (int) 0 => 'services' ) $language = 'jp' $meta_keywords = '' $meta_description = '' $meta_title = 'Human Methylome Service | Diagenode' $product = array( 'Product' => array( 'id' => '3218', 'antibody_id' => null, 'name' => 'Human Methylome Service', 'description' => '<p>NEW! <strong>Human Methylome</strong> enables high coverage genome-wide DNA methylation analysis at the single nucleotide level in Human samples and it is ideal for biomarker discovery. The assay benefits from the advantages of Twist hybrid-capture panel that targets only 83Mb genomic regions containing important DNA methylation site thus enabling a very high CpG coverage. The technology is cost-efficient since it avoids the sequencing of the whole 3.2Gb human genome which includes regions without CpGs. By using Enzymatic Methylation (EM-seq) DNA is converted without further degradation and fragmentation leading to reduced bias and homogenous targeted capture.</p><h2>High Coverage Genome-wide DNA methylation analysis</h2><ul class="square"><li>Single nucleotide resolution with enzymatic methylation (EM-seq)</li><li>Compatible with low input and highly fragmented cfDNA and FFPE</li><li>DNA inputs ranging from 200ng down to 10 ng</li><li>Robust (Hybrid-Capture) and cost-effective (reduced sequencing) solution</li><li>High coverage of 200X on CpG containing-region</li><li>Up to 18 million CpGs detected and 9 millions covered more than 10X</li><li>Covers more than 90% of the CpGs included in the Infinium EPIC array</li><li>Suitable for epigenetic biomarker discovery</li></ul><h2>Complete end-to-end service</h2><ul class="square"><li>From DNA QC to raw sequencing data</li><li>High-quality <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatic support</a> (on request) to assist you with data analysis</li><li>Leveraging</li><li>Applicable to difficult samples such as cfDNA and FFPE</li><li>High CpG average coverage thanks to enzymatic conversion and hybrid-capture</li></ul>', 'label1' => '', 'info1' => '<p>The Human Methylome Panel enables the identification and study of methylation biomarkers spanning a wide range of targets and applications. The 123 Mb panel provides a targeted NGS-based DNA methylation assay, covering more than 84% of human CpG island sites, which is 4 times the number of CpGs than other technologies and much more cost effective than WGBS. The high capture efficiency increases the sensitivity of detection and achieves a depth of coverage of 90% of bases at 30x coverage with high probe specificities.</p> <p>- Optimal service for biomarker discovery in cancer, neurodegenerative, cardiovascular and metabolic diseases<br /> - Ideal for challenging clinical samples such as liquid biopsies (plasma cfDNA) or FFPE</p> <p></p> <h2>Workflow</h2> <p><img src="https://www.diagenode.com/img/methylome-workflow.png" /></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => '', 'info2' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '', 'catalog_number' => 'G02180000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => 'on request', 'price_USD' => 'on request', 'price_GBP' => 'on request', 'price_JPY' => 'on request', 'price_CNY' => 'on request', 'price_AUD' => 'on request', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'human-methylome-service', 'meta_title' => 'Human Methylome Service | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-14 11:24:59', 'created' => '2022-04-28 15:53:29', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '1', 'position' => '9', 'parent_id' => null, 'name' => 'DNA Methylation', 'description' => '<div class="row"> <div class="large-12 columns"> <div style="text-align: justify;" class="small-12 medium-8 large-8 columns"> <h2>Complete solutions for DNA methylation studies</h2> <p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p> </div> <div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div> <div style="text-align: justify;" class="small-12 medium-12 large-12 columns"> <p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p> </div> <div class="small-12 medium-12 large-12 columns"><br /><br /> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a> <div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base. <p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p> </div> </li> </ul> <br /> <h2>Main DNA methylation technologies</h2> <p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p> <div class="row"> <ol> <li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite – Bisulfite conversion</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li> </ol> <p><span style="font-weight: 400;"> </span></p> <div class="row"> <table> <thead> <tr> <th></th> <th>Description</th> <th width="350">Features</th> </tr> </thead> <tbody> <tr> <td><strong>Bisulfite conversion</strong></td> <td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li> </ul> </td> </tr> <tr> <td><b>Methylated DNA enrichment</b></td> <td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li> </ul> </td> </tr> <tr> <td><strong>Restriction enzyme-based digestion</strong></td> <td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li> </ul> </td> </tr> </tbody> </table> </div> </div> <div class="row"></div> </div> </div> <div class="large-12 columns"></div> </div>', 'in_footer' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'epigenetics-dna-methylation', 'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation', 'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.', 'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode', 'modified' => '2019-03-25 10:07:27', 'created' => '2015-05-03 13:47:53', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '47', 'position' => '0', 'parent_id' => null, 'name' => '受託サービス', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'services-app', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'modified' => '2018-05-14 10:01:00', 'created' => '2017-11-20 14:54:17', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '105', 'position' => '1', 'parent_id' => '121', 'name' => 'DNA Methylation Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-4 large-4 columns"> <div class="panel"><center><img src="http://www.diagenode.com/img/categories/services/dna-workflow.png" alt="DNA-methylation -Diagenode" /></center> <p>The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.</p> </div> <center><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></center></div> <div class="small-12 medium-8 large-8 columns"> <p>Our Epigenomics Profiling Services helps you study DNA methylation (5mC) and methylation variants (5hmC). Our experienced scientists will guide you to the best solution according to your needs, depending on whether you want to analyze DNA methylation at genome-wide or gene specific scales. They will process the arrays or generate meaningful libraries for DNA sequencing and deliver high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</p> <h3><a href="https://www.diagenode.com/en/p/infinium-methylation-epic-array-v2-service">Infinium MethylationEPIC Array Service V2</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 930,000 CpGs detected in human samples at single nucleotide resolution</li> <li>Quantitative interrogation of CpG, non-CpG, and CHH sites</li> <li>Differential methylation analysis <span>using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> <!--<h3><a href="https://www.diagenode.com/en/p/infinium-mouse-methylation-array-service">Infinium Mouse Methylation Array Service</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 285,000 markers detected in mouse samples at single nucleotide resolution</li> <li>Suitable for common laboratory mouse strains</li> <li><span>Differential methylation analysis using our<span> </span><a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul>--> <h3><a href="https://www.diagenode.com/en/p/rrbs-service">Reduced Representation Bisulfite Sequencing (RRBS) Service</a></h3> <ul> <li>Robust and cost-effective solution with reliable results</li> <li>Unique technology enables low DNA inputs down to 25ng</li> <li>Accurate analyses with UDIs and UMIs</li> <li>Up to 7 million CpGs detected in human samples at single nucleotide resolution</li> <li>Detection of methylation patterns in CpG-rich regions across the genome including promoters and CpG islands</li> <li><span>Differential methylation analysis <span>using our</span> <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> <li>Suitable for epigenetic biomarker discovery</li> </ul> <h3><a href="https://www.diagenode.com/en/p/wgbs-service">Whole Genome Bisulfite Sequencing (WGBS) or Enzymatic Methylation(EM-seq) Service</a></h3> <ul> <li>Very powerful solution for genome-wide biomarker discovery using bisulfite or enzymatic conversion</li> <li><span>Evaluation of methylation status of nearly every CpG sites of the entire genome </span>at single nucleotide resolution</li> <li>Detection of global methylation patterns including in low CpG-density regions and outside of CpG islands</li> <li>Identification of regions or even loci with differential methylation levels between groups using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></li> </ul> <h3><a href="https://www.diagenode.com/en/p/targeted-dna-methylation-service">Targeted Bisulfite Sequencing Service</a></h3> <ul> <li>Focus on D<span>NA methylation patterning of selective regions with single nucleotide resolution</span></li> <li><span>Choose between two methodologies depending on the size of the region of interest: PCR enrichment or hybridization capture</span></li> <li><span>Enhanced accuracy, sensitivity and specificity while reducing overall cost</span></li> <li><span>Allowing deep sequencing at high coverage levels for rare variant identification</span></li> </ul> <!--<h3><a href="../p/medip-seq-service">Methylated DNA IP Sequencing</a></h3> <ul> <li>Immunoprecipitation assay (antibody-based approach) to study 5-mC genome-wide</li> <li>Resolution of 100-500 bp</li> </ul>--> <h3><a href="../p/human-methylome-service">Human Methylome</a></h3> <h3>Watch our webinar:</h3> <p>Cost-Effective Genome Wide DNA Methylation Analysis using Twist Hybrid-Capture Methylome Panel</p> <a class="popup-youtube" href="#webinar"><img src="https://www.diagenode.com/img/webinar/eshg-miniature.png" class="webinar" alt="Webinar series: Genome Wide DNA Methylation using Twist Hybrid-Capture Methylome Panel" /></a> <p></p> <ul> <li>NGS service assay for comprehensive DNA methylation profiling in human samples from solid or liquid biopsy</li> <li>Optimal biomarker discovery tool for cancer, neurodegenerative, cardiovascular and metabolic diseases</li> <li>High coverage for more than 3.89 million methylation sites (~9 million CpGs at single strand level)</li> </ul> </div> </div>', 'no_promo' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'dna-methylation-profiling-services', 'cookies_tag_id' => '3', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'Diagenode DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.', 'meta_title' => 'DNA Methylation Profiling Services EPIC array, RRBS, WGBS, MeDIP/hMeDIP-seq and Targeted DNA methylation analysis | Diagenode', 'modified' => '2023-10-04 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'services' ), 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'Human Methylome Service | Diagenode', 'product' => array( 'Product' => array( 'id' => '3218', 'antibody_id' => null, 'name' => 'Human Methylome Service', 'description' => '<p>NEW! <strong>Human Methylome</strong> enables high coverage genome-wide DNA methylation analysis at the single nucleotide level in Human samples and it is ideal for biomarker discovery. The assay benefits from the advantages of Twist hybrid-capture panel that targets only 83Mb genomic regions containing important DNA methylation site thus enabling a very high CpG coverage. The technology is cost-efficient since it avoids the sequencing of the whole 3.2Gb human genome which includes regions without CpGs. By using Enzymatic Methylation (EM-seq) DNA is converted without further degradation and fragmentation leading to reduced bias and homogenous targeted capture.</p><h2>High Coverage Genome-wide DNA methylation analysis</h2><ul class="square"><li>Single nucleotide resolution with enzymatic methylation (EM-seq)</li><li>Compatible with low input and highly fragmented cfDNA and FFPE</li><li>DNA inputs ranging from 200ng down to 10 ng</li><li>Robust (Hybrid-Capture) and cost-effective (reduced sequencing) solution</li><li>High coverage of 200X on CpG containing-region</li><li>Up to 18 million CpGs detected and 9 millions covered more than 10X</li><li>Covers more than 90% of the CpGs included in the Infinium EPIC array</li><li>Suitable for epigenetic biomarker discovery</li></ul><h2>Complete end-to-end service</h2><ul class="square"><li>From DNA QC to raw sequencing data</li><li>High-quality <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatic support</a> (on request) to assist you with data analysis</li><li>Leveraging</li><li>Applicable to difficult samples such as cfDNA and FFPE</li><li>High CpG average coverage thanks to enzymatic conversion and hybrid-capture</li></ul>', 'label1' => '', 'info1' => '<p>The Human Methylome Panel enables the identification and study of methylation biomarkers spanning a wide range of targets and applications. The 123 Mb panel provides a targeted NGS-based DNA methylation assay, covering more than 84% of human CpG island sites, which is 4 times the number of CpGs than other technologies and much more cost effective than WGBS. The high capture efficiency increases the sensitivity of detection and achieves a depth of coverage of 90% of bases at 30x coverage with high probe specificities.</p> <p>- Optimal service for biomarker discovery in cancer, neurodegenerative, cardiovascular and metabolic diseases<br /> - Ideal for challenging clinical samples such as liquid biopsies (plasma cfDNA) or FFPE</p> <p></p> <h2>Workflow</h2> <p><img src="https://www.diagenode.com/img/methylome-workflow.png" /></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => '', 'info2' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '', 'catalog_number' => 'G02180000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => 'on request', 'price_USD' => 'on request', 'price_GBP' => 'on request', 'price_JPY' => 'on request', 'price_CNY' => 'on request', 'price_AUD' => 'on request', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'human-methylome-service', 'meta_title' => 'Human Methylome Service | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-14 11:24:59', 'created' => '2022-04-28 15:53:29', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array(), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $carrousel_order = array( (int) 0 => 'services' ) $language = 'jp' $meta_keywords = '' $meta_description = '' $meta_title = 'Human Methylome Service | Diagenode' $product = array( 'Product' => array( 'id' => '3218', 'antibody_id' => null, 'name' => 'Human Methylome Service', 'description' => '<p>NEW! <strong>Human Methylome</strong> enables high coverage genome-wide DNA methylation analysis at the single nucleotide level in Human samples and it is ideal for biomarker discovery. The assay benefits from the advantages of Twist hybrid-capture panel that targets only 83Mb genomic regions containing important DNA methylation site thus enabling a very high CpG coverage. The technology is cost-efficient since it avoids the sequencing of the whole 3.2Gb human genome which includes regions without CpGs. By using Enzymatic Methylation (EM-seq) DNA is converted without further degradation and fragmentation leading to reduced bias and homogenous targeted capture.</p><h2>High Coverage Genome-wide DNA methylation analysis</h2><ul class="square"><li>Single nucleotide resolution with enzymatic methylation (EM-seq)</li><li>Compatible with low input and highly fragmented cfDNA and FFPE</li><li>DNA inputs ranging from 200ng down to 10 ng</li><li>Robust (Hybrid-Capture) and cost-effective (reduced sequencing) solution</li><li>High coverage of 200X on CpG containing-region</li><li>Up to 18 million CpGs detected and 9 millions covered more than 10X</li><li>Covers more than 90% of the CpGs included in the Infinium EPIC array</li><li>Suitable for epigenetic biomarker discovery</li></ul><h2>Complete end-to-end service</h2><ul class="square"><li>From DNA QC to raw sequencing data</li><li>High-quality <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatic support</a> (on request) to assist you with data analysis</li><li>Leveraging</li><li>Applicable to difficult samples such as cfDNA and FFPE</li><li>High CpG average coverage thanks to enzymatic conversion and hybrid-capture</li></ul>', 'label1' => '', 'info1' => '<p>The Human Methylome Panel enables the identification and study of methylation biomarkers spanning a wide range of targets and applications. The 123 Mb panel provides a targeted NGS-based DNA methylation assay, covering more than 84% of human CpG island sites, which is 4 times the number of CpGs than other technologies and much more cost effective than WGBS. The high capture efficiency increases the sensitivity of detection and achieves a depth of coverage of 90% of bases at 30x coverage with high probe specificities.</p> <p>- Optimal service for biomarker discovery in cancer, neurodegenerative, cardiovascular and metabolic diseases<br /> - Ideal for challenging clinical samples such as liquid biopsies (plasma cfDNA) or FFPE</p> <p></p> <h2>Workflow</h2> <p><img src="https://www.diagenode.com/img/methylome-workflow.png" /></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => '', 'info2' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '', 'catalog_number' => 'G02180000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => 'on request', 'price_USD' => 'on request', 'price_GBP' => 'on request', 'price_JPY' => 'on request', 'price_CNY' => 'on request', 'price_AUD' => 'on request', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'human-methylome-service', 'meta_title' => 'Human Methylome Service | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-14 11:24:59', 'created' => '2022-04-28 15:53:29', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '1', 'position' => '9', 'parent_id' => null, 'name' => 'DNA Methylation', 'description' => '<div class="row"> <div class="large-12 columns"> <div style="text-align: justify;" class="small-12 medium-8 large-8 columns"> <h2>Complete solutions for DNA methylation studies</h2> <p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p> </div> <div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div> <div style="text-align: justify;" class="small-12 medium-12 large-12 columns"> <p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p> </div> <div class="small-12 medium-12 large-12 columns"><br /><br /> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a> <div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base. <p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p> </div> </li> </ul> <br /> <h2>Main DNA methylation technologies</h2> <p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p> <div class="row"> <ol> <li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite – Bisulfite conversion</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li> </ol> <p><span style="font-weight: 400;"> </span></p> <div class="row"> <table> <thead> <tr> <th></th> <th>Description</th> <th width="350">Features</th> </tr> </thead> <tbody> <tr> <td><strong>Bisulfite conversion</strong></td> <td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li> </ul> </td> </tr> <tr> <td><b>Methylated DNA enrichment</b></td> <td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li> </ul> </td> </tr> <tr> <td><strong>Restriction enzyme-based digestion</strong></td> <td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li> </ul> </td> </tr> </tbody> </table> </div> </div> <div class="row"></div> </div> </div> <div class="large-12 columns"></div> </div>', 'in_footer' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'epigenetics-dna-methylation', 'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation', 'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.', 'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode', 'modified' => '2019-03-25 10:07:27', 'created' => '2015-05-03 13:47:53', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '47', 'position' => '0', 'parent_id' => null, 'name' => '受託サービス', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'services-app', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'modified' => '2018-05-14 10:01:00', 'created' => '2017-11-20 14:54:17', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '105', 'position' => '1', 'parent_id' => '121', 'name' => 'DNA Methylation Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-4 large-4 columns"> <div class="panel"><center><img src="http://www.diagenode.com/img/categories/services/dna-workflow.png" alt="DNA-methylation -Diagenode" /></center> <p>The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.</p> </div> <center><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></center></div> <div class="small-12 medium-8 large-8 columns"> <p>Our Epigenomics Profiling Services helps you study DNA methylation (5mC) and methylation variants (5hmC). Our experienced scientists will guide you to the best solution according to your needs, depending on whether you want to analyze DNA methylation at genome-wide or gene specific scales. They will process the arrays or generate meaningful libraries for DNA sequencing and deliver high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</p> <h3><a href="https://www.diagenode.com/en/p/infinium-methylation-epic-array-v2-service">Infinium MethylationEPIC Array Service V2</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 930,000 CpGs detected in human samples at single nucleotide resolution</li> <li>Quantitative interrogation of CpG, non-CpG, and CHH sites</li> <li>Differential methylation analysis <span>using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> <!--<h3><a href="https://www.diagenode.com/en/p/infinium-mouse-methylation-array-service">Infinium Mouse Methylation Array Service</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 285,000 markers detected in mouse samples at single nucleotide resolution</li> <li>Suitable for common laboratory mouse strains</li> <li><span>Differential methylation analysis using our<span> </span><a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul>--> <h3><a href="https://www.diagenode.com/en/p/rrbs-service">Reduced Representation Bisulfite Sequencing (RRBS) Service</a></h3> <ul> <li>Robust and cost-effective solution with reliable results</li> <li>Unique technology enables low DNA inputs down to 25ng</li> <li>Accurate analyses with UDIs and UMIs</li> <li>Up to 7 million CpGs detected in human samples at single nucleotide resolution</li> <li>Detection of methylation patterns in CpG-rich regions across the genome including promoters and CpG islands</li> <li><span>Differential methylation analysis <span>using our</span> <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> <li>Suitable for epigenetic biomarker discovery</li> </ul> <h3><a href="https://www.diagenode.com/en/p/wgbs-service">Whole Genome Bisulfite Sequencing (WGBS) or Enzymatic Methylation(EM-seq) Service</a></h3> <ul> <li>Very powerful solution for genome-wide biomarker discovery using bisulfite or enzymatic conversion</li> <li><span>Evaluation of methylation status of nearly every CpG sites of the entire genome </span>at single nucleotide resolution</li> <li>Detection of global methylation patterns including in low CpG-density regions and outside of CpG islands</li> <li>Identification of regions or even loci with differential methylation levels between groups using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></li> </ul> <h3><a href="https://www.diagenode.com/en/p/targeted-dna-methylation-service">Targeted Bisulfite Sequencing Service</a></h3> <ul> <li>Focus on D<span>NA methylation patterning of selective regions with single nucleotide resolution</span></li> <li><span>Choose between two methodologies depending on the size of the region of interest: PCR enrichment or hybridization capture</span></li> <li><span>Enhanced accuracy, sensitivity and specificity while reducing overall cost</span></li> <li><span>Allowing deep sequencing at high coverage levels for rare variant identification</span></li> </ul> <!--<h3><a href="../p/medip-seq-service">Methylated DNA IP Sequencing</a></h3> <ul> <li>Immunoprecipitation assay (antibody-based approach) to study 5-mC genome-wide</li> <li>Resolution of 100-500 bp</li> </ul>--> <h3><a href="../p/human-methylome-service">Human Methylome</a></h3> <h3>Watch our webinar:</h3> <p>Cost-Effective Genome Wide DNA Methylation Analysis using Twist Hybrid-Capture Methylome Panel</p> <a class="popup-youtube" href="#webinar"><img src="https://www.diagenode.com/img/webinar/eshg-miniature.png" class="webinar" alt="Webinar series: Genome Wide DNA Methylation using Twist Hybrid-Capture Methylome Panel" /></a> <p></p> <ul> <li>NGS service assay for comprehensive DNA methylation profiling in human samples from solid or liquid biopsy</li> <li>Optimal biomarker discovery tool for cancer, neurodegenerative, cardiovascular and metabolic diseases</li> <li>High coverage for more than 3.89 million methylation sites (~9 million CpGs at single strand level)</li> </ul> </div> </div>', 'no_promo' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'dna-methylation-profiling-services', 'cookies_tag_id' => '3', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'Diagenode DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.', 'meta_title' => 'DNA Methylation Profiling Services EPIC array, RRBS, WGBS, MeDIP/hMeDIP-seq and Targeted DNA methylation analysis | Diagenode', 'modified' => '2023-10-04 16:04:25', 'created' => '2015-11-23 14:29:35', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array( [maximum depth reached] ) ) ), 'Document' => array(), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'services' ), 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'Human Methylome Service | Diagenode', 'product' => array( 'Product' => array( 'id' => '3218', 'antibody_id' => null, 'name' => 'Human Methylome Service', 'description' => '<p>NEW! <strong>Human Methylome</strong> enables high coverage genome-wide DNA methylation analysis at the single nucleotide level in Human samples and it is ideal for biomarker discovery. The assay benefits from the advantages of Twist hybrid-capture panel that targets only 83Mb genomic regions containing important DNA methylation site thus enabling a very high CpG coverage. The technology is cost-efficient since it avoids the sequencing of the whole 3.2Gb human genome which includes regions without CpGs. By using Enzymatic Methylation (EM-seq) DNA is converted without further degradation and fragmentation leading to reduced bias and homogenous targeted capture.</p><h2>High Coverage Genome-wide DNA methylation analysis</h2><ul class="square"><li>Single nucleotide resolution with enzymatic methylation (EM-seq)</li><li>Compatible with low input and highly fragmented cfDNA and FFPE</li><li>DNA inputs ranging from 200ng down to 10 ng</li><li>Robust (Hybrid-Capture) and cost-effective (reduced sequencing) solution</li><li>High coverage of 200X on CpG containing-region</li><li>Up to 18 million CpGs detected and 9 millions covered more than 10X</li><li>Covers more than 90% of the CpGs included in the Infinium EPIC array</li><li>Suitable for epigenetic biomarker discovery</li></ul><h2>Complete end-to-end service</h2><ul class="square"><li>From DNA QC to raw sequencing data</li><li>High-quality <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatic support</a> (on request) to assist you with data analysis</li><li>Leveraging</li><li>Applicable to difficult samples such as cfDNA and FFPE</li><li>High CpG average coverage thanks to enzymatic conversion and hybrid-capture</li></ul>', 'label1' => '', 'info1' => '<p>The Human Methylome Panel enables the identification and study of methylation biomarkers spanning a wide range of targets and applications. The 123 Mb panel provides a targeted NGS-based DNA methylation assay, covering more than 84% of human CpG island sites, which is 4 times the number of CpGs than other technologies and much more cost effective than WGBS. The high capture efficiency increases the sensitivity of detection and achieves a depth of coverage of 90% of bases at 30x coverage with high probe specificities.</p> <p>- Optimal service for biomarker discovery in cancer, neurodegenerative, cardiovascular and metabolic diseases<br /> - Ideal for challenging clinical samples such as liquid biopsies (plasma cfDNA) or FFPE</p> <p></p> <h2>Workflow</h2> <p><img src="https://www.diagenode.com/img/methylome-workflow.png" /></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => '', 'info2' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '', 'catalog_number' => 'G02180000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => 'on request', 'price_USD' => 'on request', 'price_GBP' => 'on request', 'price_JPY' => 'on request', 'price_CNY' => 'on request', 'price_AUD' => 'on request', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'human-methylome-service', 'meta_title' => 'Human Methylome Service | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-14 11:24:59', 'created' => '2022-04-28 15:53:29', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array(), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $carrousel_order = array( (int) 0 => 'services' ) $language = 'jp' $meta_keywords = '' $meta_description = '' $meta_title = 'Human Methylome Service | Diagenode' $product = array( 'Product' => array( 'id' => '3218', 'antibody_id' => null, 'name' => 'Human Methylome Service', 'description' => '<p>NEW! <strong>Human Methylome</strong> enables high coverage genome-wide DNA methylation analysis at the single nucleotide level in Human samples and it is ideal for biomarker discovery. The assay benefits from the advantages of Twist hybrid-capture panel that targets only 83Mb genomic regions containing important DNA methylation site thus enabling a very high CpG coverage. The technology is cost-efficient since it avoids the sequencing of the whole 3.2Gb human genome which includes regions without CpGs. By using Enzymatic Methylation (EM-seq) DNA is converted without further degradation and fragmentation leading to reduced bias and homogenous targeted capture.</p><h2>High Coverage Genome-wide DNA methylation analysis</h2><ul class="square"><li>Single nucleotide resolution with enzymatic methylation (EM-seq)</li><li>Compatible with low input and highly fragmented cfDNA and FFPE</li><li>DNA inputs ranging from 200ng down to 10 ng</li><li>Robust (Hybrid-Capture) and cost-effective (reduced sequencing) solution</li><li>High coverage of 200X on CpG containing-region</li><li>Up to 18 million CpGs detected and 9 millions covered more than 10X</li><li>Covers more than 90% of the CpGs included in the Infinium EPIC array</li><li>Suitable for epigenetic biomarker discovery</li></ul><h2>Complete end-to-end service</h2><ul class="square"><li>From DNA QC to raw sequencing data</li><li>High-quality <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatic support</a> (on request) to assist you with data analysis</li><li>Leveraging</li><li>Applicable to difficult samples such as cfDNA and FFPE</li><li>High CpG average coverage thanks to enzymatic conversion and hybrid-capture</li></ul>', 'label1' => '', 'info1' => '<p>The Human Methylome Panel enables the identification and study of methylation biomarkers spanning a wide range of targets and applications. The 123 Mb panel provides a targeted NGS-based DNA methylation assay, covering more than 84% of human CpG island sites, which is 4 times the number of CpGs than other technologies and much more cost effective than WGBS. The high capture efficiency increases the sensitivity of detection and achieves a depth of coverage of 90% of bases at 30x coverage with high probe specificities.</p> <p>- Optimal service for biomarker discovery in cancer, neurodegenerative, cardiovascular and metabolic diseases<br /> - Ideal for challenging clinical samples such as liquid biopsies (plasma cfDNA) or FFPE</p> <p></p> <h2>Workflow</h2> <p><img src="https://www.diagenode.com/img/methylome-workflow.png" /></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => '', 'info2' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '', 'catalog_number' => 'G02180000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => 'on request', 'price_USD' => 'on request', 'price_GBP' => 'on request', 'price_JPY' => 'on request', 'price_CNY' => 'on request', 'price_AUD' => 'on request', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'human-methylome-service', 'meta_title' => 'Human Methylome Service | Diagenode', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-10-14 11:24:59', 'created' => '2022-04-28 15:53:29', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '1', 'position' => '9', 'parent_id' => null, 'name' => 'DNA Methylation', 'description' => '<div class="row"> <div class="large-12 columns"> <div style="text-align: justify;" class="small-12 medium-8 large-8 columns"> <h2>Complete solutions for DNA methylation studies</h2> <p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p> </div> <div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div> <div style="text-align: justify;" class="small-12 medium-12 large-12 columns"> <p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p> </div> <div class="small-12 medium-12 large-12 columns"><br /><br /> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a> <div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base. <p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p> </div> </li> </ul> <br /> <h2>Main DNA methylation technologies</h2> <p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p> <div class="row"> <ol> <li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite – Bisulfite conversion</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li> </ol> <p><span style="font-weight: 400;"> </span></p> <div class="row"> <table> <thead> <tr> <th></th> <th>Description</th> <th width="350">Features</th> </tr> </thead> <tbody> <tr> <td><strong>Bisulfite conversion</strong></td> <td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li> </ul> </td> </tr> <tr> <td><b>Methylated DNA enrichment</b></td> <td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li> </ul> </td> </tr> <tr> <td><strong>Restriction enzyme-based digestion</strong></td> <td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li> </ul> </td> </tr> </tbody> </table> </div> </div> <div class="row"></div> </div> </div> <div class="large-12 columns"></div> </div>', 'in_footer' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'epigenetics-dna-methylation', 'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation', 'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.', 'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode', 'modified' => '2019-03-25 10:07:27', 'created' => '2015-05-03 13:47:53', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '47', 'position' => '0', 'parent_id' => null, 'name' => '受託サービス', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'services-app', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'modified' => '2018-05-14 10:01:00', 'created' => '2017-11-20 14:54:17', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '105', 'position' => '1', 'parent_id' => '121', 'name' => 'DNA Methylation Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-4 large-4 columns"> <div class="panel"><center><img src="http://www.diagenode.com/img/categories/services/dna-workflow.png" alt="DNA-methylation -Diagenode" /></center> <p>The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.</p> </div> <center><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></center></div> <div class="small-12 medium-8 large-8 columns"> <p>Our Epigenomics Profiling Services helps you study DNA methylation (5mC) and methylation variants (5hmC). Our experienced scientists will guide you to the best solution according to your needs, depending on whether you want to analyze DNA methylation at genome-wide or gene specific scales. They will process the arrays or generate meaningful libraries for DNA sequencing and deliver high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</p> <h3><a href="https://www.diagenode.com/en/p/infinium-methylation-epic-array-v2-service">Infinium MethylationEPIC Array Service V2</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 930,000 CpGs detected in human samples at single nucleotide resolution</li> <li>Quantitative interrogation of CpG, non-CpG, and CHH sites</li> <li>Differential methylation analysis <span>using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> <!--<h3><a href="https://www.diagenode.com/en/p/infinium-mouse-methylation-array-service">Infinium Mouse Methylation Array Service</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 285,000 markers detected in mouse samples at single nucleotide resolution</li> <li>Suitable for common laboratory mouse strains</li> <li><span>Differential methylation analysis using our<span> </span><a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul>--> <h3><a href="https://www.diagenode.com/en/p/rrbs-service">Reduced Representation Bisulfite Sequencing (RRBS) Service</a></h3> <ul> <li>Robust and cost-effective solution with reliable results</li> <li>Unique technology enables low DNA inputs down to 25ng</li> <li>Accurate analyses with UDIs and UMIs</li> <li>Up to 7 million CpGs detected in human samples at single nucleotide resolution</li> <li>Detection of methylation patterns in CpG-rich regions across the genome including promoters and CpG islands</li> <li><span>Differential methylation analysis <span>using our</span> <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> <li>Suitable for epigenetic biomarker discovery</li> </ul> <h3><a href="https://www.diagenode.com/en/p/wgbs-service">Whole Genome Bisulfite Sequencing (WGBS) or Enzymatic Methylation(EM-seq) Service</a></h3> <ul> <li>Very powerful solution for genome-wide biomarker discovery using bisulfite or enzymatic conversion</li> <li><span>Evaluation of methylation status of nearly every CpG sites of the entire genome </span>at single nucleotide resolution</li> <li>Detection of global methylation patterns including in low CpG-density regions and outside of CpG islands</li> <li>Identification of regions or even loci with differential methylation levels between groups using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></li> </ul> <h3><a href="https://www.diagenode.com/en/p/targeted-dna-methylation-service">Targeted Bisulfite Sequencing Service</a></h3> <ul> <li>Focus on D<span>NA methylation patterning of selective regions with single nucleotide resolution</span></li> <li><span>Choose between two methodologies depending on the size of the region of interest: PCR enrichment or hybridization capture</span></li> <li><span>Enhanced accuracy, sensitivity and specificity while reducing overall cost</span></li> <li><span>Allowing deep sequencing at high coverage levels for rare variant identification</span></li> </ul> <!--<h3><a href="../p/medip-seq-service">Methylated DNA IP Sequencing</a></h3> <ul> <li>Immunoprecipitation assay (antibody-based approach) to study 5-mC genome-wide</li> <li>Resolution of 100-500 bp</li> </ul>--> <h3><a href="../p/human-methylome-service">Human Methylome</a></h3> <h3>Watch our webinar:</h3> <p>Cost-Effective Genome Wide DNA Methylation Analysis using Twist Hybrid-Capture Methylome Panel</p> <a class="popup-youtube" href="#webinar"><img src="https://www.diagenode.com/img/webinar/eshg-miniature.png" class="webinar" alt="Webinar series: Genome Wide DNA Methylation using Twist Hybrid-Capture Methylome Panel" /></a> <p></p> <ul> <li>NGS service assay for comprehensive DNA methylation profiling in human samples from solid or liquid biopsy</li> <li>Optimal biomarker discovery tool for cancer, neurodegenerative, cardiovascular and metabolic diseases</li> <li>High coverage for more than 3.89 million methylation sites (~9 million CpGs at single strand level)</li> </ul> </div> </div>', 'no_promo' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'dna-methylation-profiling-services', 'cookies_tag_id' => '3', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'Diagenode DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.', 'meta_title' => 'DNA Methylation Profiling Services EPIC array, RRBS, WGBS, MeDIP/hMeDIP-seq and Targeted DNA methylation analysis | Diagenode', 'modified' => '2023-10-04 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The assay benefits from the advantages of Twist hybrid-capture panel that targets only 83Mb genomic regions containing important DNA methylation site thus enabling a very high CpG coverage. The technology is cost-efficient since it avoids the sequencing of the whole 3.2Gb human genome which includes regions without CpGs. By using Enzymatic Methylation (EM-seq) DNA is converted without further degradation and fragmentation leading to reduced bias and homogenous targeted capture.</p><h2>High Coverage Genome-wide DNA methylation analysis</h2><ul class="square"><li>Single nucleotide resolution with enzymatic methylation (EM-seq)</li><li>Compatible with low input and highly fragmented cfDNA and FFPE</li><li>DNA inputs ranging from 200ng down to 10 ng</li><li>Robust (Hybrid-Capture) and cost-effective (reduced sequencing) solution</li><li>High coverage of 200X on CpG containing-region</li><li>Up to 18 million CpGs detected and 9 millions covered more than 10X</li><li>Covers more than 90% of the CpGs included in the Infinium EPIC array</li><li>Suitable for epigenetic biomarker discovery</li></ul><h2>Complete end-to-end service</h2><ul class="square"><li>From DNA QC to raw sequencing data</li><li>High-quality <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatic support</a> (on request) to assist you with data analysis</li><li>Leveraging</li><li>Applicable to difficult samples such as cfDNA and FFPE</li><li>High CpG average coverage thanks to enzymatic conversion and hybrid-capture</li></ul>', 'label1' => '', 'info1' => '<p>The Human Methylome Panel enables the identification and study of methylation biomarkers spanning a wide range of targets and applications. 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The assay benefits from the advantages of Twist hybrid-capture panel that targets only 83Mb genomic regions containing important DNA methylation site thus enabling a very high CpG coverage. The technology is cost-efficient since it avoids the sequencing of the whole 3.2Gb human genome which includes regions without CpGs. 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Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p> </div> <div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div> <div style="text-align: justify;" class="small-12 medium-12 large-12 columns"> <p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p> </div> <div class="small-12 medium-12 large-12 columns"><br /><br /> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a> <div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base. <p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p> </div> </li> </ul> <br /> <h2>Main DNA methylation technologies</h2> <p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p> <div class="row"> <ol> <li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite – Bisulfite conversion</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li> </ol> <p><span style="font-weight: 400;"> </span></p> <div class="row"> <table> <thead> <tr> <th></th> <th>Description</th> <th width="350">Features</th> </tr> </thead> <tbody> <tr> <td><strong>Bisulfite conversion</strong></td> <td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li> </ul> </td> </tr> <tr> <td><b>Methylated DNA enrichment</b></td> <td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li> </ul> </td> </tr> <tr> <td><strong>Restriction enzyme-based digestion</strong></td> <td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td> <td> <ul style="list-style-type: circle;"> <li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li> </ul> </td> </tr> </tbody> </table> </div> </div> <div class="row"></div> </div> </div> <div class="large-12 columns"></div> </div>', 'in_footer' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'epigenetics-dna-methylation', 'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation', 'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.', 'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode', 'modified' => '2019-03-25 10:07:27', 'created' => '2015-05-03 13:47:53', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '47', 'position' => '0', 'parent_id' => null, 'name' => '受託サービス', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'services-app', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'modified' => '2018-05-14 10:01:00', 'created' => '2017-11-20 14:54:17', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '105', 'position' => '1', 'parent_id' => '121', 'name' => 'DNA Methylation Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-4 large-4 columns"> <div class="panel"><center><img src="http://www.diagenode.com/img/categories/services/dna-workflow.png" alt="DNA-methylation -Diagenode" /></center> <p>The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.</p> </div> <center><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></center></div> <div class="small-12 medium-8 large-8 columns"> <p>Our Epigenomics Profiling Services helps you study DNA methylation (5mC) and methylation variants (5hmC). Our experienced scientists will guide you to the best solution according to your needs, depending on whether you want to analyze DNA methylation at genome-wide or gene specific scales. They will process the arrays or generate meaningful libraries for DNA sequencing and deliver high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</p> <h3><a href="https://www.diagenode.com/en/p/infinium-methylation-epic-array-v2-service">Infinium MethylationEPIC Array Service V2</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 930,000 CpGs detected in human samples at single nucleotide resolution</li> <li>Quantitative interrogation of CpG, non-CpG, and CHH sites</li> <li>Differential methylation analysis <span>using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> <!--<h3><a href="https://www.diagenode.com/en/p/infinium-mouse-methylation-array-service">Infinium Mouse Methylation Array Service</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 285,000 markers detected in mouse samples at single nucleotide resolution</li> <li>Suitable for common laboratory mouse strains</li> <li><span>Differential methylation analysis using our<span> </span><a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul>--> <h3><a href="https://www.diagenode.com/en/p/rrbs-service">Reduced Representation Bisulfite Sequencing (RRBS) Service</a></h3> <ul> <li>Robust and cost-effective solution with reliable results</li> <li>Unique technology enables low DNA inputs down to 25ng</li> <li>Accurate analyses with UDIs and UMIs</li> <li>Up to 7 million CpGs detected in human samples at single nucleotide resolution</li> <li>Detection of methylation patterns in CpG-rich regions across the genome including promoters and CpG islands</li> <li><span>Differential methylation analysis <span>using our</span> <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> <li>Suitable for epigenetic biomarker discovery</li> </ul> <h3><a href="https://www.diagenode.com/en/p/wgbs-service">Whole Genome Bisulfite Sequencing (WGBS) or Enzymatic Methylation(EM-seq) Service</a></h3> <ul> <li>Very powerful solution for genome-wide biomarker discovery using bisulfite or enzymatic conversion</li> <li><span>Evaluation of methylation status of nearly every CpG sites of the entire genome </span>at single nucleotide resolution</li> <li>Detection of global methylation patterns including in low CpG-density regions and outside of CpG islands</li> <li>Identification of regions or even loci with differential methylation levels between groups using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></li> </ul> <h3><a href="https://www.diagenode.com/en/p/targeted-dna-methylation-service">Targeted Bisulfite Sequencing Service</a></h3> <ul> <li>Focus on D<span>NA methylation patterning of selective regions with single nucleotide resolution</span></li> <li><span>Choose between two methodologies depending on the size of the region of interest: PCR enrichment or hybridization capture</span></li> <li><span>Enhanced accuracy, sensitivity and specificity while reducing overall cost</span></li> <li><span>Allowing deep sequencing at high coverage levels for rare variant identification</span></li> </ul> <!--<h3><a href="../p/medip-seq-service">Methylated DNA IP Sequencing</a></h3> <ul> <li>Immunoprecipitation assay (antibody-based approach) to study 5-mC genome-wide</li> <li>Resolution of 100-500 bp</li> </ul>--> <h3><a href="../p/human-methylome-service">Human Methylome</a></h3> <h3>Watch our webinar:</h3> <p>Cost-Effective Genome Wide DNA Methylation Analysis using Twist Hybrid-Capture Methylome Panel</p> <a class="popup-youtube" href="#webinar"><img src="https://www.diagenode.com/img/webinar/eshg-miniature.png" class="webinar" alt="Webinar series: Genome Wide DNA Methylation using Twist Hybrid-Capture Methylome Panel" /></a> <p></p> <ul> <li>NGS service assay for comprehensive DNA methylation profiling in human samples from solid or liquid biopsy</li> <li>Optimal biomarker discovery tool for cancer, neurodegenerative, cardiovascular and metabolic diseases</li> <li>High coverage for more than 3.89 million methylation sites (~9 million CpGs at single strand level)</li> </ul> </div> </div>', 'no_promo' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'dna-methylation-profiling-services', 'cookies_tag_id' => '3', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'Diagenode DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.', 'meta_title' => 'DNA Methylation Profiling Services EPIC array, RRBS, WGBS, MeDIP/hMeDIP-seq and Targeted DNA methylation analysis | Diagenode', 'modified' => '2023-10-04 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