Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:
Workflow |
Description |
Region of interest |
---|---|---|
PCR Enrichment |
|
|
Hybridization Capture |
|
|
Targeted bisulfite sequencing enables DNA methylation analysis of targeted genomic regions with single nucleotide resolution. Depending on the size of the region of interest, the methodology may include a PCR-based step to amplify multiple bisulfite-converted DNA regions in a single reaction (PCR enrichment workflow) or a hybridization capture step using pre-designed oligos to isolate DNA regions that contain target CpG sites (hybridization capture workflow).
This method is useful for hypothesis testing studies of target regions of interest as well as confirmation of regions identified in genome-wide methylation analyses such as WGBS, RRBS, and MeDIP-seq.