Targeted DNA methylation analysis allows for the quantitative study of cytosine methylation at known and precise locations throughout the genome. Depending on the size of the region of interest, a number of methodologies can be employed including bisulfite pyrosequencing, bisulfite amplicon sequencing (BSAS), and custom capturing with probes. These techniques share a common methodology of bisulfite conversion followed by sequencing in order to quantitatively detect the methylation status of the target regions at single nucleotide resolution.
|
Targeted DNA methylation service |
Description |
Best adapted applications |
|
Bisulfite Pyrosequencing  |
|
Small number of single CpGs or short DNA regions with most of the researcher’s CpGs of interest |
|
Bisulfite Amplicon Sequencing (BSAS)  |
|
Several, long DNA regions -- total size up to 200kb |
|
Custom capture probes  |
|
Large number of very long DNA regions -- total size between 0.2-24Mb |
 How to properly cite this product in your workDiagenode strongly recommends using this: Targeted DNA methylation service (Diagenode Cat# G02070000). Click here to copy to clipboard. Using our products in your publication? Let us know! |
The European Regional Development Fund and Wallonia are investing in your future.
Extension of industrial buildings and new laboratories.
