Targeted DNA methylation service

Targeted DNA methylation service	Description	Best adapted applications
Bisulfite Pyrosequencing	Bisulfite-converted DNA regions PCR-amplified. One PCR strand sequenced on PyroMark Assay design, primers, and PCR conditions validated Species: human, mouse, rat, and monkey	Small number of single CpGs or short DNA regions with most of the researcher’s CpGs of interest
Bisulfite Amplicon Sequencing (BSAS)	Bisulfite-converted DNA and target region(s) amplified with several PCR amplicons. Amplicons sequenced using Illumina® platform. Assay, primer design, PCR conditions optimized Species: human	Several, long DNA regions -- total size up to 200kb
Custom capture probes	Target region(s) selectively captured with custom-designed RNA baits (Agilent SureSelect). Captured regions are bisulfite-converted and sequenced on Illumina® platform. Species: human	Large number of very long DNA regions -- total size between 0.2-24Mb

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Differential methylation analysis for any service is available upon request.

Diagenode strongly recommends using this: Targeted DNA methylation service (Diagenode Cat# G02070000). Click here to copy to clipboard.

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