Targeted Bisulfite Sequencing Service

Targeted bisulfite sequencing enables DNA methylation analysis of targeted genomic regions with single nucleotide resolution. Depending on the size of the region of interest, the methodology may include a PCR-based step to amplify multiple bisulfite-converted DNA regions in a single reaction (PCR enrichment workflow) or a hybridization capture step using pre-designed oligos to isolate DNA regions that contain target CpG sites (hybridization capture workflow).

This method is useful for hypothesis testing studies of target regions of interest as well as confirmation of regions identified in genome-wide methylation analyses such as WGBS, RRBS, and MeDIP-seq.

 DNA methylation with focused content

• DNA methylation patterning of selective regions
• Single nucleotide resolution
• Enhanced accuracy, sensitivity and specificity while reducing overall cost
• Allowing deep sequencing at high coverage levels for rare variant identification
• Suitable for epigenetic biomarker validation 

Customized end-to-end service

• From project design to data analysis
• A dedicated scientist driving your project with high touch communication
• High-quality bioinformatic support to assist you with data analysis

PCR enrichment worflow

Hybridization capture worflow

See our other DNA Methylation Profiling Services