Diagenode

Targeted DNA methylation service

default alt
  •  描述
  •  文档
  •  相关
  •   获得报价
目录号
格式
G02070000

Targeted DNA methylation analysis allows for the quantitative study of cytosine methylation at known and precise locations throughout the genome. Depending on the size of the region of interest, a number of methodologies can be employed including bisulfite pyrosequencing, bisulfite amplicon sequencing (BSAS), and custom capturing with probes. These techniques share a common methodology of bisulfite conversion followed by sequencing in order to quantitatively detect the methylation status of the target regions at single nucleotide resolution.

  • Description

    Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:

    Workflow

    Description

    Region of interest

    PCR Enrichment

    • (gDNA shearing on Bioruptor Pico)
    • Bisulfite conversion
    • PCR primer design
    • PCR amplification optimization
    • Library preparation
    • QC of final libraries (DNA concentration, analysis of the profile)
    • Sequencing on Illumina platform
    • Several, long DNA regions
    • Total size up to 200kb

    Hybridization Capture

    • (gDNA shearing on Bioruptor Pico)
    • Bisulfite conversion
    • Library preparation
    • Capture probe design
    • Amplification optimization
    • QC of final libraries (DNA concentration, analysis of the profile)
    • Sequencing on Illumina platform
    • Large number of very long DNA regions
    • Total size between 0.2-100Mb
  • Bioinformatic analysis

    Analysis

    Features

    Standard
    • FASTQ raw data
    • FASTQC quality control insights
    • Alignment of bisulfite sequencing data against reference genome
    • Methylation calling and extraction
    • Summary statistics
    Differential methylation analysis
    • Methylation level analysis
    • Differentially Methylated CpGs (DMCs) analysis
    • Differentially Methylated Regions (DMRs) analysis
    • Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)

  •  文档
    Epigenomics Profiling Services FLYER
    Chromatin analysis DNA methylation services RNA-seq analysis
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Targeted DNA methylation service (Diagenode Cat# G02070000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  相关产品

活动

  • 64th ASH Annual Meeting and Exposition 2022
    New Orleans, Louisiana, USA
    Dec 10-Dec 13, 2022
 查看所有活动

新闻

 查看所有新闻


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics