RRBS Service includes:
|QC of the genomic DNA||
|Preparation of RRBS libraries||
This figure shows the perfect match obtained between the expected versus the observed efficiency of the MspI enzyme used in the Premium RRBS kit. The four upper tracks represent two samples (control and treatment). The first and second tracks show respectively, the read coverage and the detected methylation for each sample. The height of the methylation bars is equivalent to the percentage methylation. The MspI track shows the in-silico digestion of gDNA.
RRBS data generated with the Diagenode RRBS service using samples from four different species
|Species||No. of reads aligned (alignment rate)||No. of unique CpGs covered (alignment rate)||Bisulfite conversion rate (%)||Mean sequencing depth per covered CpG (×)|
|Human FFPE||27,290,117 (79%)||2,525,053||99.7||28|
Reduced representation bisulfite sequencing (RRBS) enables genome-scale DNA methylation analysis in any vertebrate species. The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of whole-genome sequencing. The Diagenode RRBS service makes this technology widely available and provides high coverage (up to 4 million CpGs in human samples). Multiplexing prior to bisulfite conversion allows processing of 96 samples per experiment, enabling studies of large cohorts.
We are very happy with the services provided by Diagenode. In our project, Reduced Representation Bisulfite Sequencing (RRBS) was used and it worked well for both high-quality DNA and DNA extracted from formalin-fixed, paraffin-embedded (FFPE) material. Bioinformatic analyses were comprehensive and high-quality results were obtained. Diagenode’s services can be recommended.Satu Mäki-Nevala, PhD, University of Helsinki