Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:
Workflow |
Description |
Region of interest |
|---|---|---|
|
PCR Enrichment |
|
|
|
Hybridization Capture |
|
|
You are about to request a quote for our epigenomics services. Fill out the form below and we will be in touch with you very soon.
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Targeted DNA methylation 受託サービスは、ゲノム全体の既知の正確な位置でのシトシンメチル化の定量的研究を可能にします。関心領域の大きさに応じて、バイサルファイトパイロシーケンシング、バイサルファイトアンプリコンシーケンシング（BSAS）、およびプローブによるカスタムキャプチャーなどの数多くの方法論を用いることが可能です。これらの技術は、単一ヌクレオチド分解能で標的領域のメチル化状態を定量的に検出するために、バイサルファイト変換とその後のシーケンシングに共通の方法論を共有しています。
Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:
Workflow |
Description |
Region of interest |
|---|---|---|
|
PCR Enrichment |
|
|
|
Hybridization Capture |
|
|
Analysis |
Features |
|---|---|
| Standard |
|
| Differential methylation analysis |
|
| Epigenomics Profiling Services FLYER Chromatin analysis DNA methylation services RNA-seq analysis | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: Targeted DNA methylation 受託サービス (Diagenode Cat# G02070000). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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You are about to request a quote for our epigenomics services. Fill out the form below and we will be in touch with you very soon.
All * fields are mandatory
You are about to request a quote for our epigenomics services. Fill out the form below and we will be in touch with you very soon.
All * fields are mandatory
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line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Targeted DNA methylation 受託サービスは、ゲノム全体の既知の正確な位置でのシトシンメチル化の定量的研究を可能にします。関心領域の大きさに応じて、バイサルファイトパイロシーケンシング、バイサルファイトアンプリコンシーケンシング（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">BSAS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）、およびプローブによるカスタムキャプチャーなどの数多くの方法論を用いることが可能です。これらの技術は、単一ヌクレオチド分解能で標的領域のメチル化状態を定量的に検出するために、<em>バイサルファイト変換</em>とその後のシーケンシングに共通の方法論を共有しています。</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; color: #222222;"><o:p></o:p></span></p>', 'label1' => '詳細', 'info1' => '<p style="text-align: left;">Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:</p> <table style="width: 941px;"> <thead> <tr style="height: 69px;"> <th style="width: 218px; height: 69px;"> <h4><strong>Workflow</strong></h4> </th> <th style="width: 402px; 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encrypted-media" allowfullscreen="allowfullscreen"></iframe></center> <p></p> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <blockquote style="padding-bottom: 0;"><span class="label-green" style="margin-bottom: 16px; margin-left: -22px; font-size: 22px;">WHITE PAPERS</span> <div id="portal" class="main-portal"> <div class="portal-inner"><nav class="portal-nav" style="text-align: left;"> <ul data-tab="" class="tips-menu"> <li><a href="#panel1" class="tips portal button">Smokers vs non-smokers </a></li> <li><a href="#panel2" class="tips portal button">Breast cancer</a></li> </ul> </nav></div> </div> <div class="tabs-content"> <div class="content active" id="panel1"> <h3 style="margin-top: 0;">Powerful new insights with epigenetic data mining.<br /> A study to distinguish smokers from non-smokers using just one droplet of blood</h3> <p>Next generation sequencing in combination with sophisticated bioinformatics technologies for genomic, transcriptomic and epigeneomic analyses have enormous potential to establish new biomarkers for disease diagnostics, enabling true precision medicine. Analyses of liquid biopsies to measure thousands of different data points simultaneously in easily accessible body fluids (e.g. blood, urine, and saliva) are extremely promising for such biomarker studies.<br />(...)</p> <a href="https://www.diagenode.com/en/pages/form-bioinfo" class="alert small button" target="_blank">Read more</a></div> <div class="content" id="panel2"> <h3 style="margin-top: 0;">Data mining on DNA methylation data in cancer samples<br />Distinguishing normal from breast cancer tissue</h3> <p>Breast cancer is the most commonly occurring cancer in women and the second most common cancer overall.</p> <p>One important aspect of cancer tissues it that they differ from normal tissues in their epigenetic make up, especially in the DNA methylation pattern. In normal cells methylation assures the proper regulation of gene expression and stable gene silencing. DNA methylation is associated with histone modifications, and the interplay of these epigenetic modifications is crucial to regulate the functioning of the genome by changing chromatin architecture.<br />(...)</p> <a href="https://www.diagenode.com/en/pages/form-bioinfo?app_note=23" class="alert small button" target="_blank">Read more</a></div> </div> </blockquote> </div> </div> <p>Diagenode's new data mining service utilizes methods at the frontier of machine learning, statistics, and database systems. This enhanced service supports new discoveries that were previously not possible by analyzing patterns in large data sets to give informative new insights.</p> <p>If you have data from patient cohorts, single cell analyses or any other heterogeneous scenarios, our service team provides enhanced support with optimal data analysis using our latest data mining capabilities. Specifically, our team applies machine learning technologies to find previously undiscovered or unobvious relationships within and across datasets. This advanced technology allows discovery of informative features from mass data, essentially “finding a needle in a haystack.”</p> <p>Diagenode utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data. Our goal is to build strong classifiers that separate data into two or more classes or groups depending on associated data.</p> <p class="extra-spaced">Different and multiple -omics data classes can be mined simultaneously. Integration with phenotypic and/or clinical data is also possible. We offer data mining services for several data classes including:</p> <table class="extra-spaced"> <tbody> <tr> <td><strong>Epigenetic data</strong></td> <td><strong>Transcriptomic data</strong></td> </tr> <tr> <td> <p>DNA Methylation (RRBS, WGBS, EPIC arrays)</p> <p>ChIP-sequencing</p> <p>ATAC-seq</p> </td> <td> <p>mRNA-sequencing</p> <p>Small and long non coding RNA</p> <p>Single-cell RNA-sequencing</p> </td> </tr> </tbody> </table> <p></p> <p><strong>Biological Interpretation</strong></p> <p class="extra-spaced">Machine learning classifiers also mirror the underlying biological differences between classes and are used to uncover the molecular processes at work. In order to achieve this, we offer biological interpretation services and pathway mining analyses for your data.</p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Data mining modules ', 'info1' => '<ol> <li><strong>Feasibility study</strong></li> </ol> <ul> <li>Assessment of data characteristics and <span></span>applicability of different machine learning (ML)</li> <li>Prototypic analyses:</li> <li>Initial feasibility report</li> </ul> <p></p> <ol start="2"> <li><strong>Data Mining</strong></li> </ol> <ul> <li>Machine learning on data</li> <li>Data evaluation and validation</li> <li>Report generation</li> </ul> <p> </p> <ol start="3"> <li><strong>Data Interpretation</strong></li> </ol> <ul> <li>Integration of background knowledge</li> <li>Functional interpretation / pathway mining</li> <li>Scientific reporting</li> </ul> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'Integration with wet lab services and bioinformatics', 'info2' => '<p>A clear advantage of <strong>Diagenode’s data mining services</strong> is the close connection with<span> </span>other service offerings like <strong>wet lab analysis services</strong> and <strong>bioinformatics services</strong>. You can retrieve a full service package from a single source.</p> <p><a href="https://www.diagenode.com/en/categories/Services">Read about our wetlab services</a></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => 'Custom', 'catalog_number' => 'G02100000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'data-mining-service', 'meta_title' => 'Bioinformatics Data Mining Service | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Diagenode Data Mining Services utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data.', 'modified' => '2020-12-21 08:05:15', 'created' => '2019-04-08 16:43:26', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2989', 'antibody_id' => null, 'name' => 'WGBS (Whole Genome Bisulfite Sequencing) and EM-seq (Enzymatic Methylation)受託サービス ', 'description' => '<p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Whole-genome bisulfite sequencing（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">WGBS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）は、</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">DNA</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">メチル化プロファイリングの中でも最も包括的な次世代シーケンシングであり、ゲノム全体で</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">5-mC</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">の単一塩基分解能を可能にします。</span></p> <p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">同じ場所での変換されていないシトシンと変換されたシトシンの割合を比較することにより、メチル化レベルが決定されます。</span></p> <p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;"></span></p> <pre class="tw-data-text tw-text-large tw-ta" data-placeholder="Translation" id="tw-target-text" dir="ltr"><span class="Y2IQFc" lang="ja"></span><span lang="EN" style="font-family: 'Arial Unicode MS'; color: #b21329;">DNA</span><span lang="FR" style="font-family: 'Arial Unicode MS'; color: #b21329;">メチル化研究において最も高いカバレッジ</span></pre> <ul> <li class="normal"><span>ゲノム全体のDNAメチル化と単一塩基分解能スクリーニング</span></li> <li class="normal">低密度と反復領域における5mCの高カバレッジ</li> <li class="normal">専任の科学者がハイタッチコミュニケーションで各プロジェクトをサポート</li> <li class="normal">包括的なサービス - バイサルファイト処理、ライブラリーの調製、シーケンシングと解析</li> </ul> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '詳細', 'info1' => '<h4>WGBS Service includes:</h4> <table style="width: 942px;"> <tbody> <tr style="text-align: left; height: 81px;"> <td style="width: 469px; height: 81px;" colspan="2"> <p><strong>QC of the genomic DNA</strong></p> <p><strong></strong></p> </td> <td style="width: 766px; height: 81px;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">Measurement of DNA concentration</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Assessment of DNA quality</span></li> </ul> </td> </tr> <tr style="height: 129px;"> <td style="width: 375px; height: 236px;" rowspan="2"> <p style="text-align: left;"><strong>Library Preparation</strong></p> <p style="text-align: left;"><strong></strong></p> </td> <td style="width: 94px; height: 129px;"> <p style="text-align: center;"><strong>WGBS</strong></p> </td> <td style="width: 766px; height: 129px;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">gDNA shearing on Bioruptor Pico (not necessary for cfDNA or FFPE)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Bisulfite conversion</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Library preparation </span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">QC of the WGBS libraries (DNA concentration, analysis of the profile)</span></li> </ul> </td> </tr> <tr style="height: 107px;"> <td style="width: 94px; text-align: center; height: 107px; background-color: #f9f9f9;"><strong>EM-seq</strong></td> <td style="width: 766px; height: 107px; background-color: #f9f9f9;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">gDNA shearing on Bioruptor Pico (not necessary for cfDNA or FFPE)</span></li> <li style="font-weight: 400;">Library preparation with Enzymatic conversion</li> <li style="font-weight: 400;">QC of EM-seq libraries (DNA concentration, analysis of the profile)</li> </ul> <span style="font-weight: 400;"></span></td> </tr> <tr style="height: 129px;"> <td style="width: 469px; height: 129px; background-color: white;" colspan="2"> <p><strong>Deep sequencing</strong></p> <p><strong></strong></p> </td> <td style="width: 766px; height: 129px; background-color: white;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;"><span>Samples are sequenced on Illumina platform, paired-end reads of 150bp length (PE150) </span> </span></li> <li style="font-weight: 400;">400M raw reads on average per samples (when pooling 6 samples/lane)</li> <li style="font-weight: 400;">Theoretical Coverage >30X for human, mouse and rat samples</li> <li style="font-weight: 400;"><span style="font-weight: 400;">Detection of >50 million CpGs with 6-9X average CpG coverage for human samples </span></li> </ul> </td> </tr> </tbody> </table> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'バイオインフォマティック解析', 'info2' => '<table style="width: 895px;"> <thead> <tr style="height: 69px;"> <th style="width: 262px; height: 69px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px; height: 69px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr style="height: 123px;"> <td style="width: 262px; height: 123px;"><strong>Standard</strong></td> <td style="width: 624px; height: 123px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr style="height: 146px;"> <td style="width: 262px; height: 146px;"><strong>Differential methylation analysis</strong></td> <td style="width: 624px; height: 146px;"> <ul> <li>Methylation level analysis</li> <li>Differentially methylated CpGs (DMCs) analysis</li> <li>Differentially methylated regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr style="height: 82px;"> <td style="width: 262px; height: 82px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px; height: 82px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr style="height: 86px;"> <td style="width: 262px; height: 86px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px; height: 86px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> <tr style="height: 86px;"> <td style="width: 262px; height: 86px;"> <p><strong>Data mining</strong></p> </td> <td style="width: 624px; height: 86px;"> <ul> <li><span>Biomarker discovery</span></li> <li><span>Determination of methylation pattern/signature that can be predictive and discriminate between different groups/conditions</span></li> </ul> </td> </tr> </tbody> </table> <h2></h2> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '', 'catalog_number' => 'G02040000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'wgbs-service', 'meta_title' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'meta_keywords' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'meta_description' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'modified' => '2022-04-11 04:55:23', 'created' => '2018-09-06 10:51:07', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '2836', 'antibody_id' => null, 'name' => 'RRBS Service (Reduced Representation Bisulfite Sequencing)', 'description' => '<p><a href="https://www.diagenode.com/en/quotes/quote?id=2836"><img alt="RRBS Service" src="https://www.diagenode.com/img/banners/b-page-promo-rrbs.png" /></a></p> <div class="extra-spaced"> <p><strong>Reduced representation bisulfite sequencing (RRBS) </strong> <span>enables </span><span>genome-s</span><span>cale </span>DNA methylation<span> analysis</span> at the single nucleotide level <span>in any vertebrate species. </span><span>The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of</span> whole genome sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent<span> biologically relevant target</span> CpG-rich regions including <span>promoters and </span>CpG islands.<span> Our RRBS service makes this technology widely available and provides high coverage (up to 7 million CpGs</span><span> detected </span><span>in human samples).</span></p> <h2>Genome-scale DNA methylation analysis</h2> <ul class="square"> <li>Robust and cost-effective solution with reliable results</li> <li>Capability to use gDNA inputs down to 25 ng</li> <li>Use of UDIs enhance sequencing results</li> <li>UMI technology eliminates PCR duplicates</li> <li>High coverage - up to 7 million CpGs detected in human samples</li> <li>Single nucleotide resolution</li> <li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li> <li>Suitable for any vertebrate species</li> </ul> <h2>Complete end-to-end service</h2> <ul class="square"> <li><span><span class="ui-provider ee cfi bsv cfj cfk cfl cfm cfn cfo cfp cfq cfr cfs cft cfu cfv cfw cfx cfy cfz cga cgb cgc cgd cge cgf cgg cgh cgi cgj cgk cgl cgm cgn cgo" dir="ltr">From DNA QC to sequencing raw data</span></span></li> <li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2</a> widely adopted</li> <li>As little as 25 ng for any vertebrate species</li> <li>Differentially methylated site analysis <span>using <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> </div> <div class="extra-spaced"> <h2></h2> </div> <p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>', 'label1' => 'Services Workflow', 'info1' => '<div class="row"> <div class="small-6 columns"> <h4>RRBS Service includes:</h4> <table style="width: 905px;"> <tbody> <tr> <td style="width: 264px;"><strong>QC of the genomic DNA</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Measurement of DNA concentration </li> <li>Assessment of DNA quality</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>MspI digestion</li> <li>Library preparation (ends preparation, adaptor ligation)</li> <li>Size selection</li> <li>Sample pooling</li> <li>Bisulfite conversion</li> <li>Library amplification and clean-up</li> <li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Deep sequencing</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li> <li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li> <li>7 million CpGs (on average) for human samples</li> <li>7-11x CpG coverage (on average) for human samples</li> </ul> </td> </tr> </tbody> </table> </div> </div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Bioinformatics Analysis', 'info2' => '<div class="row"> <div class="small-6 columns"> <table style="width: 895px;"> <thead> <tr> <th style="width: 262px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr> <td style="width: 262px;"><strong>Standard</strong></td> <td style="width: 624px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr> <td style="width: 262px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> </tbody> </table> </div> </div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '', 'catalog_number' => 'G02020000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'rrbs-service', 'meta_title' => 'RRBS Service (Reduced Representation Bisulfite Sequencing) | Diagenode', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'RRBS enables genome-scale DNA methylation analysis at the single nucleotide level in any vertebrate species. Robust and cost-effective solution with high coverage (up to 5 million CpGs in human samples).', 'modified' => '2024-01-10 17:48:39', 'created' => '2016-09-21 16:54:44', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array([maximum depth reached]) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '105', 'position' => '1', 'parent_id' => '121', 'name' => 'DNA Methylation Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-4 large-4 columns"> <div class="panel"><center><img src="http://www.diagenode.com/img/categories/services/dna-workflow.png" alt="DNA-methylation -Diagenode" /></center> <p>The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.</p> </div> <center><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></center></div> <div class="small-12 medium-8 large-8 columns"> <p>Our Epigenomics Profiling Services helps you study DNA methylation (5mC) and methylation variants (5hmC). Our experienced scientists will guide you to the best solution according to your needs, depending on whether you want to analyze DNA methylation at genome-wide or gene specific scales. They will process the arrays or generate meaningful libraries for DNA sequencing and deliver high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</p> <h3><a href="https://www.diagenode.com/en/p/infinium-methylation-epic-array-v2-service">Infinium MethylationEPIC Array Service V2</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 930,000 CpGs detected in human samples at single nucleotide resolution</li> <li>Quantitative interrogation of CpG, non-CpG, and CHH sites</li> <li>Differential methylation analysis <span>using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> <!--<h3><a href="https://www.diagenode.com/en/p/infinium-mouse-methylation-array-service">Infinium Mouse Methylation Array Service</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 285,000 markers detected in mouse samples at single nucleotide resolution</li> <li>Suitable for common laboratory mouse strains</li> <li><span>Differential methylation analysis using our<span> </span><a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul>--> <h3><a href="https://www.diagenode.com/en/p/rrbs-service">Reduced Representation Bisulfite Sequencing (RRBS) Service</a></h3> <ul> <li>Robust and cost-effective solution with reliable results</li> <li>Unique technology enables low DNA inputs down to 25ng</li> <li>Accurate analyses with UDIs and UMIs</li> <li>Up to 7 million CpGs detected in human samples at single nucleotide resolution</li> <li>Detection of methylation patterns in CpG-rich regions across the genome including promoters and CpG islands</li> <li><span>Differential methylation analysis <span>using our</span> <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> <li>Suitable for epigenetic biomarker discovery</li> </ul> <h3><a href="https://www.diagenode.com/en/p/wgbs-service">Whole Genome Bisulfite Sequencing (WGBS) or Enzymatic Methylation(EM-seq) Service</a></h3> <ul> <li>Very powerful solution for genome-wide biomarker discovery using bisulfite or enzymatic conversion</li> <li><span>Evaluation of methylation status of nearly every CpG sites of the entire genome </span>at single nucleotide resolution</li> <li>Detection of global methylation patterns including in low CpG-density regions and outside of CpG islands</li> <li>Identification of regions or even loci with differential methylation levels between groups using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></li> </ul> <h3><a href="https://www.diagenode.com/en/p/targeted-dna-methylation-service">Targeted Bisulfite Sequencing Service</a></h3> <ul> <li>Focus on D<span>NA methylation patterning of selective regions with single nucleotide resolution</span></li> <li><span>Choose between two methodologies depending on the size of the region of interest: PCR enrichment or hybridization capture</span></li> <li><span>Enhanced accuracy, sensitivity and specificity while reducing overall cost</span></li> <li><span>Allowing deep sequencing at high coverage levels for rare variant identification</span></li> </ul> <!--<h3><a href="../p/medip-seq-service">Methylated DNA IP Sequencing</a></h3> <ul> <li>Immunoprecipitation assay (antibody-based approach) to study 5-mC genome-wide</li> <li>Resolution of 100-500 bp</li> </ul>--> <h3><a href="../p/human-methylome-service">Human Methylome</a></h3> <h3>Watch our webinar:</h3> <p>Cost-Effective Genome Wide DNA Methylation Analysis using Twist Hybrid-Capture Methylome Panel</p> <a class="popup-youtube" href="#webinar"><img src="https://www.diagenode.com/img/webinar/eshg-miniature.png" class="webinar" alt="Webinar series: Genome Wide DNA Methylation using Twist Hybrid-Capture Methylome Panel" /></a> <p></p> <ul> <li>NGS service assay for comprehensive DNA methylation profiling in human samples from solid or liquid biopsy</li> <li>Optimal biomarker discovery tool for cancer, neurodegenerative, cardiovascular and metabolic diseases</li> <li>High coverage for more than 3.89 million methylation sites (~9 million CpGs at single strand level)</li> </ul> </div> </div>', 'no_promo' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'dna-methylation-profiling-services', 'cookies_tag_id' => '3', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'Diagenode DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.', 'meta_title' => 'DNA Methylation Profiling Services EPIC array, RRBS, WGBS, MeDIP/hMeDIP-seq and Targeted DNA methylation analysis | Diagenode', 'modified' => '2023-10-04 16:04:25', 'created' => '2015-11-23 14:29:35', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '121', 'position' => '9', 'parent_id' => null, 'name' => 'Epigenomics Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-10 large-10 columns text-left"> <p>Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis. </p> </div> <div class="small-12 medium-2 large-2 columns text-left"><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></div> </div> <center><img alt="DNA-RNA-Chromatin-Diagenode" src="https://www.diagenode.com/img/categories/services/chromatin-dna-rna.png" /></center> <div class="row"> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #099f92;"> <h3><span class="green">Chromatin</span> analysis</h3> <ul> <li>ChIP-seq analysis Histone modification</li> <li>Promoter analysis</li> <li>Enhancer analysis</li> <li>ChIP-seq analysis Transcription factor </li> <li>(sc)ATAC-seq Chromatin accessibility</li> <li>ChIP-seq Cas9 off-target</li> <li>Customized NGS service</li> </ul> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/chip-seq-service">Learn more</a></p> </div> </div> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #474546;"> <h3><span class="darkgrey">DNA methylation</span> analysis</h3> <ul> <li>Reduced representation bisulfite sequencing (RRBS)</li> <li>Whole genome bisulfite sequencing (WGBS)</li> <li>Targeted methylation</li> <li>MeDIP-seq/hMeDIP-seq</li> <li>Infinium MethylationEPIC array </li> </ul> <p></p> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/dna-methylation-profiling-services">Learn more</a></p> </div> </div> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #b11e33;"> <h3><span class="diacol"><g class="gr_ gr_44 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="44" data-gr-id="44">RNA-seq</g></span> analysis</h3> <ul> <li><span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;">Gene expression profiling</span></li> <li>mRNA<span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;"> analysis</span></li> <li>Small non-coding RNA analysis</li> <li><b></b>Whole transcriptome analysis<br /><br /><br /><br /><br /></li> </ul> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/rna-seq-category">Learn more</a></p> </div> </div> </div> <h2 style="text-align: center;"><a class="details radius small button" href="https://www.diagenode.com/en/documents/services-flyer">DOWNLOAD OUR FLYER</a></h2> <h2>Why Diagenode</h2> <ul> <li><strong>Expertise and trust</strong>: Recognized epigenetics leader, official partner of <a href="http://www.blueprint-epigenome.eu/"><g class="gr_ gr_45 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="45" data-gr-id="45">BLUEPRINT</g></a>, <a href="http://ihec-epigenomes.org/">IHEC</a> <g class="gr_ gr_46 gr-alert gr_gramm gr_inline_cards gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="46" data-gr-id="46">and</g> <a href="https://www.faang.org/">FAANG</a></li> <li><strong>Innovative technology</strong>: Utilization of the signature Bioruptor<sup>®</sup> sonication device for optimal chromatin and DNA shearing and the IP-Star<sup>®</sup> Automation device give reproducible and reliable optimization and results</li> <li><strong>Quality</strong>: Multiple QC steps in all workflows and validated antibodies plus reagents deliver superior data</li> <li><strong>Flexibility</strong>: Extensive range of sample species and sample origins</li> <li><strong>Experience</strong> in epigenomics profiling for a wide range of applications and fields of interest</li> </ul> <div class="extra-spaced"> <ul class="small-block-grid-2 medium-block-grid-3 large-block-grid-3"> <li> <div class="block-distri"><span class="label-green" style="margin-bottom: 16px; 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columns"> <input name="data[Quote][state]" id="state-3022" maxlength="3" type="text"/><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/> </div> </div> <div class="row collapse" id="email_v"> <div class="small-3 large-2 columns"> <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Comment</span> </div> <div class="small-9 large-10 columns"> <textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha66296bb0109ac"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-3022" class="alert button expand" form="Quote-3022" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-3022 #state-3022").parent().parent().show(); } else { $("#Quote-3022 #state-3022").parent().parent().hide(); $("#Quote-3022 #state-3022").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3022" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="data-mining-service" data-reveal-id="quoteModal-3022" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">データマイニングサービス</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/wgbs-service"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">G02040000</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2989" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>our epigenomics services</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=2989" id="Quote-2989" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2989" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RGxyN3VUNERGenBPUExVK3k0L0Ivdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="wgbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Service Information</h2> </div> <div class="small-12 large-12 columns"> <h4>Which services are you interested in?</h4> </div> <div class="small-12 large-12 columns"> <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium MethylationEPIC Array v2" id="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2" /><label for="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2">Infinium MethylationEPIC Array v2</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Human Methylome" id="QuoteEpigenomicsServiceHumanMethylome" /><label for="QuoteEpigenomicsServiceHumanMethylome">Human Methylome</label></div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-3 columns"> <span class="prefix">Sample species</span> </div> <div class="small-12 medium-12 large-9 columns"> <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/> </div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-6 columns"> <span class="prefix">Total number of samples (including replicates)</span> </div> <div class="small-12 medium-12 large-6 columns"> <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/> </div> </div> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2989"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option 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#state-2989").parent().parent().show(); } else { $("#Quote-2989 #state-2989").parent().parent().hide(); $("#Quote-2989 #state-2989").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2989" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="wgbs-service" data-reveal-id="quoteModal-2989" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">WGBS (Whole Genome Bisulfite Sequencing) and EM...</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/rrbs-service"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">G02020000</span> </div> <div class="small-6 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Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=2836" id="Quote-2836" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2836" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RGxyN3VUNERGenBPUExVK3k0L0Ivdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="rrbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Service Information</h2> </div> <div class="small-12 large-12 columns"> <h4>Which services are you interested in?</h4> </div> <div class="small-12 large-12 columns"> <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium MethylationEPIC Array v2" id="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2" /><label for="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2">Infinium MethylationEPIC Array v2</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Human Methylome" id="QuoteEpigenomicsServiceHumanMethylome" /><label for="QuoteEpigenomicsServiceHumanMethylome">Human Methylome</label></div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-3 columns"> <span class="prefix">Sample species</span> </div> <div class="small-12 medium-12 large-9 columns"> <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/> </div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-6 columns"> <span class="prefix">Total number of samples (including replicates)</span> </div> <div class="small-12 medium-12 large-6 columns"> <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/> </div> </div> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2836"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option value="AW">Aruba</option> <option value="AU">Australia</option> <option value="AT">Austria</option> <option value="AZ">Azerbaijan</option> <option value="BS">Bahamas</option> <option value="BH">Bahrain</option> <option value="BD">Bangladesh</option> <option value="BB">Barbados</option> <option value="BY">Belarus</option> <option value="BE">Belgium</option> <option value="BZ">Belize</option> <option value="BJ">Benin</option> <option value="BM">Bermuda</option> <option value="BT">Bhutan</option> <option value="BO">Bolivia</option> <option value="BQ">Bonaire, Sint Eustatius and Saba</option> <option value="BA">Bosnia and Herzegovina</option> <option value="BW">Botswana</option> <option value="BV">Bouvet Island</option> <option value="BR">Brazil</option> <option value="IO">British Indian Ocean Territory</option> <option value="BN">Brunei Darussalam</option> <option value="BG">Bulgaria</option> <option value="BF">Burkina Faso</option> <option value="BI">Burundi</option> <option value="KH">Cambodia</option> <option value="CM">Cameroon</option> <option value="CA">Canada</option> <option value="CV">Cape Verde</option> <option value="KY">Cayman Islands</option> <option value="CF">Central African Republic</option> <option value="TD">Chad</option> <option value="CL">Chile</option> <option value="CN">China</option> <option value="CX">Christmas Island</option> <option value="CC">Cocos (Keeling) Islands</option> <option value="CO">Colombia</option> <option value="KM">Comoros</option> <option value="CG">Congo</option> <option value="CD">Congo, The Democratic Republic of the</option> <option value="CK">Cook Islands</option> <option value="CR">Costa Rica</option> <option value="CI">Côte d'Ivoire</option> <option value="HR">Croatia</option> <option value="CU">Cuba</option> <option value="CW">Curaçao</option> <option value="CY">Cyprus</option> <option value="CZ">Czech Republic</option> <option value="DK">Denmark</option> <option value="DJ">Djibouti</option> <option value="DM">Dominica</option> <option value="DO">Dominican Republic</option> <option value="EC">Ecuador</option> <option value="EG">Egypt</option> <option value="SV">El Salvador</option> <option value="GQ">Equatorial Guinea</option> <option value="ER">Eritrea</option> <option value="EE">Estonia</option> <option value="ET">Ethiopia</option> <option value="FK">Falkland Islands (Malvinas)</option> <option value="FO">Faroe Islands</option> <option value="FJ">Fiji</option> <option value="FI">Finland</option> <option value="FR">France</option> <option value="GF">French Guiana</option> <option value="PF">French Polynesia</option> <option value="TF">French Southern Territories</option> <option value="GA">Gabon</option> <option value="GM">Gambia</option> <option value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option value="GD">Grenada</option> <option value="GP">Guadeloupe</option> <option value="GU">Guam</option> <option value="GT">Guatemala</option> <option value="GG">Guernsey</option> <option value="GN">Guinea</option> <option value="GW">Guinea-Bissau</option> <option value="GY">Guyana</option> <option value="HT">Haiti</option> <option value="HM">Heard Island and McDonald Islands</option> <option value="VA">Holy See (Vatican City State)</option> <option value="HN">Honduras</option> <option value="HK">Hong Kong</option> <option value="HU">Hungary</option> <option value="IS">Iceland</option> <option value="IN">India</option> <option value="ID">Indonesia</option> <option value="IR">Iran, Islamic Republic of</option> <option value="IQ">Iraq</option> <option value="IE">Ireland</option> <option value="IM">Isle of Man</option> <option value="IL">Israel</option> <option value="IT">Italy</option> <option value="JM">Jamaica</option> <option value="JP">Japan</option> <option value="JE">Jersey</option> <option value="JO">Jordan</option> <option value="KZ">Kazakhstan</option> <option value="KE">Kenya</option> <option value="KI">Kiribati</option> <option value="KP">Korea, Democratic People's Republic of</option> <option value="KR">Korea, Republic of</option> <option value="KW">Kuwait</option> <option value="KG">Kyrgyzstan</option> <option value="LA">Lao People's Democratic Republic</option> <option value="LV">Latvia</option> <option value="LB">Lebanon</option> <option value="LS">Lesotho</option> <option value="LR">Liberia</option> <option value="LY">Libya</option> <option value="LI">Liechtenstein</option> <option value="LT">Lithuania</option> <option value="LU">Luxembourg</option> <option value="MO">Macao</option> <option value="MK">Macedonia, Republic of</option> <option value="MG">Madagascar</option> <option value="MW">Malawi</option> <option value="MY">Malaysia</option> <option value="MV">Maldives</option> <option value="ML">Mali</option> <option value="MT">Malta</option> <option value="MH">Marshall Islands</option> <option value="MQ">Martinique</option> <option value="MR">Mauritania</option> <option value="MU">Mauritius</option> <option value="YT">Mayotte</option> <option value="MX">Mexico</option> <option value="FM">Micronesia, Federated States of</option> <option value="MD">Moldova</option> <option value="MC">Monaco</option> <option value="MN">Mongolia</option> <option value="ME">Montenegro</option> <option value="MS">Montserrat</option> <option value="MA">Morocco</option> <option value="MZ">Mozambique</option> <option value="MM">Myanmar</option> <option value="NA">Namibia</option> <option value="NR">Nauru</option> <option value="NP">Nepal</option> <option value="NL">Netherlands</option> <option value="NC">New Caledonia</option> <option value="NZ">New Zealand</option> <option value="NI">Nicaragua</option> <option value="NE">Niger</option> <option value="NG">Nigeria</option> <option value="NU">Niue</option> <option value="NF">Norfolk Island</option> <option value="MP">Northern Mariana Islands</option> <option value="NO">Norway</option> <option value="OM">Oman</option> <option value="PK">Pakistan</option> <option value="PW">Palau</option> <option value="PS">Palestine, State of</option> <option value="PA">Panama</option> <option value="PG">Papua New Guinea</option> <option value="PY">Paraguay</option> <option value="PE">Peru</option> <option value="PH">Philippines</option> <option value="PN">Pitcairn</option> <option value="PL">Poland</option> <option value="PT">Portugal</option> <option value="PR">Puerto Rico</option> <option value="QA">Qatar</option> <option value="RE">Réunion</option> <option value="RO">Romania</option> <option value="RU">Russian Federation</option> <option value="RW">Rwanda</option> <option value="BL">Saint Barthélemy</option> <option value="SH">Saint Helena, Ascension and Tristan da Cunha</option> <option value="KN">Saint Kitts and Nevis</option> <option value="LC">Saint Lucia</option> <option value="MF">Saint Martin (French part)</option> <option value="PM">Saint Pierre and Miquelon</option> <option value="VC">Saint Vincent and the Grenadines</option> <option value="WS">Samoa</option> <option value="SM">San Marino</option> <option value="ST">Sao Tome and Principe</option> <option value="SA">Saudi Arabia</option> <option value="SN">Senegal</option> <option value="RS">Serbia</option> <option value="SC">Seychelles</option> <option value="SL">Sierra Leone</option> <option value="SG">Singapore</option> <option value="SX">Sint Maarten (Dutch part)</option> <option value="SK">Slovakia</option> <option value="SI">Slovenia</option> <option value="SB">Solomon Islands</option> <option value="SO">Somalia</option> <option value="ZA">South Africa</option> <option value="GS">South Georgia and the South Sandwich Islands</option> <option value="ES">Spain</option> <option value="LK">Sri Lanka</option> <option value="SD">Sudan</option> <option value="SR">Suriname</option> <option value="SS">South Sudan</option> <option value="SJ">Svalbard and Jan Mayen</option> <option value="SZ">Swaziland</option> <option value="SE">Sweden</option> <option value="CH">Switzerland</option> <option value="SY">Syrian Arab Republic</option> <option value="TW">Taiwan</option> <option value="TJ">Tajikistan</option> <option value="TZ">Tanzania</option> <option value="TH">Thailand</option> <option value="TL">Timor-Leste</option> <option value="TG">Togo</option> <option value="TK">Tokelau</option> <option value="TO">Tonga</option> <option value="TT">Trinidad and Tobago</option> <option value="TN">Tunisia</option> <option value="TR">Turkey</option> <option value="TM">Turkmenistan</option> <option value="TC">Turks and Caicos Islands</option> <option value="TV">Tuvalu</option> <option value="UG">Uganda</option> <option value="UA">Ukraine</option> <option value="AE">United Arab Emirates</option> <option value="GB">United Kingdom</option> <option value="US" selected="selected">United States</option> <option value="UM">United States Minor Outlying Islands</option> <option value="UY">Uruguay</option> <option value="UZ">Uzbekistan</option> <option value="VU">Vanuatu</option> <option value="VE">Venezuela</option> <option value="VN">Viet Nam</option> <option value="VG">Virgin Islands, British</option> <option value="VI">Virgin Islands, U.S.</option> <option value="WF">Wallis and Futuna</option> <option value="EH">Western Sahara</option> <option value="YE">Yemen</option> <option value="ZM">Zambia</option> <option value="ZW">Zimbabwe</option> </select><script> $('#country_selector_quote-2836').selectize(); </script><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">State</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][state]" id="state-2836" maxlength="3" type="text"/><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/> </div> </div> <div class="row collapse" id="email_v"> <div class="small-3 large-2 columns"> <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Project</span> </div> <div class="small-9 large-10 columns"> <textarea name="data[Quote][comment]" placeholder="Describe your project" cols="30" rows="6" id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha66296bb02896a"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-2836" class="alert button expand" form="Quote-2836" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { $('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>'); $('#Quote-2836').attr('action','javascript:postToPardot(\'' + $('#Quote-2836').attr('action') + '\', 2836)'); }); $("#Quote-2836 #submit_btn-2836").click(function (e) { if($(this).closest('form')[0].checkValidity()){ e.preventDefault(); //disable the submit button $("#Quote-2836 #submit_btn-2836").attr("disabled", true); $("#Quote-2836 #submit_btn-2836").html("Processing..."); //submit the form $("#Quote-2836").submit(); } }) </script> <script> if ($("#Quote-2836 #country_selector_quote-2836.selectized").val() == 'US') { var val = $("#state-2836").val(); $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut 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bisulfite sequencing (RRBS) </strong> <span>enables </span><span>genome-s</span><span>cale </span>DNA methylation<span> analysis</span> at the single nucleotide level <span>in any vertebrate species. </span><span>The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of</span> whole genome sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent<span> biologically relevant target</span> CpG-rich regions including <span>promoters and </span>CpG islands.<span> Our RRBS service makes this technology widely available and provides high coverage (up to 7 million CpGs</span><span> detected </span><span>in human samples).</span></p> <h2>Genome-scale DNA methylation analysis</h2> <ul class="square"> <li>Robust and cost-effective solution with reliable results</li> <li>Capability to use gDNA inputs down to 25 ng</li> <li>Use of UDIs enhance sequencing results</li> <li>UMI technology eliminates PCR duplicates</li> <li>High coverage - up to 7 million CpGs detected in human samples</li> <li>Single nucleotide resolution</li> <li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li> <li>Suitable for any vertebrate species</li> </ul> <h2>Complete end-to-end service</h2> <ul class="square"> <li><span><span class="ui-provider ee cfi bsv cfj cfk cfl cfm cfn cfo cfp cfq cfr cfs cft cfu cfv cfw cfx cfy cfz cga cgb cgc cgd cge cgf cgg cgh cgi cgj cgk cgl cgm cgn cgo" dir="ltr">From DNA QC to sequencing raw data</span></span></li> <li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2</a> widely adopted</li> <li>As little as 25 ng for any vertebrate species</li> <li>Differentially methylated site analysis <span>using <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> </div> <div class="extra-spaced"> <h2></h2> </div> <p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>', 'label1' => 'Services Workflow', 'info1' => '<div class="row"> <div class="small-6 columns"> <h4>RRBS Service includes:</h4> <table style="width: 905px;"> <tbody> <tr> <td style="width: 264px;"><strong>QC of the genomic DNA</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Measurement of DNA concentration </li> <li>Assessment of DNA quality</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>MspI digestion</li> <li>Library preparation (ends preparation, adaptor ligation)</li> <li>Size selection</li> <li>Sample pooling</li> <li>Bisulfite conversion</li> <li>Library amplification and clean-up</li> <li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Deep sequencing</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li> <li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li> <li>7 million CpGs (on average) for human samples</li> <li>7-11x CpG coverage (on average) for human samples</li> </ul> </td> </tr> </tbody> </table> </div> </div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Bioinformatics Analysis', 'info2' => '<div class="row"> <div class="small-6 columns"> <table style="width: 895px;"> <thead> <tr> <th style="width: 262px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr> <td style="width: 262px;"><strong>Standard</strong></td> <td style="width: 624px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr> <td style="width: 262px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> </tbody> </table> </div> </div> <div id="ConnectiveDocSignExtentionInstalled" 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'services' ), 'language' => 'jp', 'meta_keywords' => 'Targeted DNA methylation service', 'meta_description' => 'Targeted DNA methylation service', 'meta_title' => 'Targeted DNA methylation service', 'product' => array( 'Product' => array( 'id' => '2992', 'antibody_id' => null, 'name' => 'Targeted DNA methylation 受託サービス', 'description' => '<p class="normal" style="text-align: justify; line-height: 150%;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Targeted DNA methylation 受託サービスは、ゲノム全体の既知の正確な位置でのシトシンメチル化の定量的研究を可能にします。関心領域の大きさに応じて、バイサルファイトパイロシーケンシング、バイサルファイトアンプリコンシーケンシング（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">BSAS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）、およびプローブによるカスタムキャプチャーなどの数多くの方法論を用いることが可能です。これらの技術は、単一ヌクレオチド分解能で標的領域のメチル化状態を定量的に検出するために、<em>バイサルファイト変換</em>とその後のシーケンシングに共通の方法論を共有しています。</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; color: #222222;"><o:p></o:p></span></p>', 'label1' => '詳細', 'info1' => '<p style="text-align: left;">Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:</p> <table style="width: 941px;"> <thead> <tr style="height: 69px;"> <th style="width: 218px; height: 69px;"> <h4><strong>Workflow</strong></h4> </th> <th style="width: 402px; height: 69px;"> <h4><strong>Description</strong></h4> </th> <th style="width: 312px; height: 69px;"> <h4><strong>Region of interest</strong></h4> </th> </tr> </thead> <tbody> <tr style="height: 0;"> <td></td> <td></td> <td></td> </tr> <tr style="height: 132px; vertical-align: top;"> <td style="width: 218px; height: 132px;"> <p style="text-align: left;"><strong>PCR Enrichment</strong></p> </td> <td style="width: 402px; height: 132px;"> <ul style="list-style-type: disc;"> <li>(gDNA shearing on Bioruptor Pico)</li> <li>Bisulfite conversion</li> <li>PCR primer design</li> <li>PCR amplification optimization</li> <li>Library preparation</li> <li>QC of final libraries (DNA concentration, analysis of the profile)</li> <li>Sequencing on Illumina platform</li> </ul> </td> <td style="width: 312px; height: 132px; text-align: left;"> <ul style="list-style-type: disc;"> <li style="text-align: left;">Several, long DNA regions</li> <li style="text-align: left;">Total size up to 200kb</li> </ul> </td> </tr> <tr style="height: 157px; 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height: 123px;"><strong>Standard</strong></td> <td style="width: 624px; height: 123px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr style="height: 146px;"> <td style="width: 262px; height: 146px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px; height: 146px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)</li> </ul> </td> </tr> </tbody> </table> <h4></h4>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '', 'catalog_number' => 'G02070000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'targeted-dna-methylation-service', 'meta_title' => 'Targeted DNA methylation service', 'meta_keywords' => 'Targeted DNA methylation service', 'meta_description' => 'Targeted DNA methylation service', 'modified' => '2021-03-08 15:31:23', 'created' => '2018-09-06 10:54:45', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $carrousel_order = array( (int) 0 => 'services' ) $language = 'jp' $meta_keywords = 'Targeted DNA methylation service' $meta_description = 'Targeted DNA methylation service' $meta_title = 'Targeted DNA methylation service' $product = array( 'Product' => array( 'id' => '2992', 'antibody_id' => null, 'name' => 'Targeted DNA methylation 受託サービス', 'description' => '<p class="normal" style="text-align: justify; line-height: 150%;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Targeted DNA methylation 受託サービスは、ゲノム全体の既知の正確な位置でのシトシンメチル化の定量的研究を可能にします。関心領域の大きさに応じて、バイサルファイトパイロシーケンシング、バイサルファイトアンプリコンシーケンシング（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">BSAS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）、およびプローブによるカスタムキャプチャーなどの数多くの方法論を用いることが可能です。これらの技術は、単一ヌクレオチド分解能で標的領域のメチル化状態を定量的に検出するために、<em>バイサルファイト変換</em>とその後のシーケンシングに共通の方法論を共有しています。</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; color: #222222;"><o:p></o:p></span></p>', 'label1' => '詳細', 'info1' => '<p style="text-align: left;">Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:</p> <table style="width: 941px;"> <thead> <tr style="height: 69px;"> <th style="width: 218px; height: 69px;"> <h4><strong>Workflow</strong></h4> </th> <th style="width: 402px; height: 69px;"> <h4><strong>Description</strong></h4> </th> <th style="width: 312px; height: 69px;"> <h4><strong>Region of interest</strong></h4> </th> </tr> </thead> <tbody> <tr style="height: 0;"> <td></td> <td></td> <td></td> </tr> <tr style="height: 132px; vertical-align: top;"> <td style="width: 218px; height: 132px;"> <p style="text-align: left;"><strong>PCR Enrichment</strong></p> </td> <td style="width: 402px; height: 132px;"> <ul style="list-style-type: disc;"> <li>(gDNA shearing on Bioruptor Pico)</li> <li>Bisulfite conversion</li> <li>PCR primer design</li> <li>PCR amplification optimization</li> <li>Library preparation</li> <li>QC of final libraries (DNA concentration, analysis of the profile)</li> <li>Sequencing on Illumina platform</li> </ul> </td> <td style="width: 312px; height: 132px; text-align: left;"> <ul style="list-style-type: disc;"> <li style="text-align: left;">Several, long DNA regions</li> <li style="text-align: left;">Total size up to 200kb</li> </ul> </td> </tr> <tr style="height: 157px; 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height: 123px;"><strong>Standard</strong></td> <td style="width: 624px; height: 123px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr style="height: 146px;"> <td style="width: 262px; height: 146px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px; height: 146px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)</li> </ul> </td> </tr> </tbody> </table> <h4></h4>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '', 'catalog_number' => 'G02070000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'targeted-dna-methylation-service', 'meta_title' => 'Targeted DNA methylation service', 'meta_keywords' => 'Targeted DNA methylation service', 'meta_description' => 'Targeted DNA methylation service', 'modified' => '2021-03-08 15:31:23', 'created' => '2018-09-06 10:54:45', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '3022', 'antibody_id' => null, 'name' => 'Bioinformatics Data Mining Service', 'description' => '<h2 class="text-center"><span class="diacol">New!</span> <br />Data mining using machine learning (AI) for unique epigenetic data insights</h2> <center><img src="https://www.diagenode.com/img/product/data-mining-long.png" /></center> <p></p> <div id="paper" style="text-align: center;"> <h4><a href="https://www.youtube.com/watch?v=KXjnSHz3Jk8">Watch the webinar to gain insights on how data mining can be applied for epigenetics applications</a></h4> </div> <center><iframe width="560" height="315" src="https://www.youtube.com/embed/KXjnSHz3Jk8" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen="allowfullscreen"></iframe></center> <p></p> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <blockquote style="padding-bottom: 0;"><span class="label-green" style="margin-bottom: 16px; margin-left: -22px; font-size: 22px;">WHITE PAPERS</span> <div id="portal" class="main-portal"> <div class="portal-inner"><nav class="portal-nav" style="text-align: left;"> <ul data-tab="" class="tips-menu"> <li><a href="#panel1" class="tips portal button">Smokers vs non-smokers </a></li> <li><a href="#panel2" class="tips portal button">Breast cancer</a></li> </ul> </nav></div> </div> <div class="tabs-content"> <div class="content active" id="panel1"> <h3 style="margin-top: 0;">Powerful new insights with epigenetic data mining.<br /> A study to distinguish smokers from non-smokers using just one droplet of blood</h3> <p>Next generation sequencing in combination with sophisticated bioinformatics technologies for genomic, transcriptomic and epigeneomic analyses have enormous potential to establish new biomarkers for disease diagnostics, enabling true precision medicine. Analyses of liquid biopsies to measure thousands of different data points simultaneously in easily accessible body fluids (e.g. blood, urine, and saliva) are extremely promising for such biomarker studies.<br />(...)</p> <a href="https://www.diagenode.com/en/pages/form-bioinfo" class="alert small button" target="_blank">Read more</a></div> <div class="content" id="panel2"> <h3 style="margin-top: 0;">Data mining on DNA methylation data in cancer samples<br />Distinguishing normal from breast cancer tissue</h3> <p>Breast cancer is the most commonly occurring cancer in women and the second most common cancer overall.</p> <p>One important aspect of cancer tissues it that they differ from normal tissues in their epigenetic make up, especially in the DNA methylation pattern. In normal cells methylation assures the proper regulation of gene expression and stable gene silencing. DNA methylation is associated with histone modifications, and the interplay of these epigenetic modifications is crucial to regulate the functioning of the genome by changing chromatin architecture.<br />(...)</p> <a href="https://www.diagenode.com/en/pages/form-bioinfo?app_note=23" class="alert small button" target="_blank">Read more</a></div> </div> </blockquote> </div> </div> <p>Diagenode's new data mining service utilizes methods at the frontier of machine learning, statistics, and database systems. This enhanced service supports new discoveries that were previously not possible by analyzing patterns in large data sets to give informative new insights.</p> <p>If you have data from patient cohorts, single cell analyses or any other heterogeneous scenarios, our service team provides enhanced support with optimal data analysis using our latest data mining capabilities. Specifically, our team applies machine learning technologies to find previously undiscovered or unobvious relationships within and across datasets. This advanced technology allows discovery of informative features from mass data, essentially “finding a needle in a haystack.”</p> <p>Diagenode utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data. Our goal is to build strong classifiers that separate data into two or more classes or groups depending on associated data.</p> <p class="extra-spaced">Different and multiple -omics data classes can be mined simultaneously. Integration with phenotypic and/or clinical data is also possible. We offer data mining services for several data classes including:</p> <table class="extra-spaced"> <tbody> <tr> <td><strong>Epigenetic data</strong></td> <td><strong>Transcriptomic data</strong></td> </tr> <tr> <td> <p>DNA Methylation (RRBS, WGBS, EPIC arrays)</p> <p>ChIP-sequencing</p> <p>ATAC-seq</p> </td> <td> <p>mRNA-sequencing</p> <p>Small and long non coding RNA</p> <p>Single-cell RNA-sequencing</p> </td> </tr> </tbody> </table> <p></p> <p><strong>Biological Interpretation</strong></p> <p class="extra-spaced">Machine learning classifiers also mirror the underlying biological differences between classes and are used to uncover the molecular processes at work. In order to achieve this, we offer biological interpretation services and pathway mining analyses for your data.</p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Data mining modules ', 'info1' => '<ol> <li><strong>Feasibility study</strong></li> </ol> <ul> <li>Assessment of data characteristics and <span></span>applicability of different machine learning (ML)</li> <li>Prototypic analyses:</li> <li>Initial feasibility report</li> </ul> <p></p> <ol start="2"> <li><strong>Data Mining</strong></li> </ol> <ul> <li>Machine learning on data</li> <li>Data evaluation and validation</li> <li>Report generation</li> </ul> <p> </p> <ol start="3"> <li><strong>Data Interpretation</strong></li> </ol> <ul> <li>Integration of background knowledge</li> <li>Functional interpretation / pathway mining</li> <li>Scientific reporting</li> </ul> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'Integration with wet lab services and bioinformatics', 'info2' => '<p>A clear advantage of <strong>Diagenode’s data mining services</strong> is the close connection with<span> </span>other service offerings like <strong>wet lab analysis services</strong> and <strong>bioinformatics services</strong>. You can retrieve a full service package from a single source.</p> <p><a href="https://www.diagenode.com/en/categories/Services">Read about our wetlab services</a></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => 'Custom', 'catalog_number' => 'G02100000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'data-mining-service', 'meta_title' => 'Bioinformatics Data Mining Service | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Diagenode Data Mining Services utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data.', 'modified' => '2020-12-21 08:05:15', 'created' => '2019-04-08 16:43:26', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2989', 'antibody_id' => null, 'name' => 'WGBS (Whole Genome Bisulfite Sequencing) and EM-seq (Enzymatic Methylation)受託サービス ', 'description' => '<p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Whole-genome bisulfite sequencing（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">WGBS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）は、</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">DNA</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">メチル化プロファイリングの中でも最も包括的な次世代シーケンシングであり、ゲノム全体で</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">5-mC</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">の単一塩基分解能を可能にします。</span></p> <p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">同じ場所での変換されていないシトシンと変換されたシトシンの割合を比較することにより、メチル化レベルが決定されます。</span></p> <p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;"></span></p> <pre class="tw-data-text tw-text-large tw-ta" data-placeholder="Translation" id="tw-target-text" dir="ltr"><span class="Y2IQFc" lang="ja"></span><span lang="EN" style="font-family: 'Arial Unicode MS'; color: #b21329;">DNA</span><span lang="FR" style="font-family: 'Arial Unicode MS'; color: #b21329;">メチル化研究において最も高いカバレッジ</span></pre> <ul> <li class="normal"><span>ゲノム全体のDNAメチル化と単一塩基分解能スクリーニング</span></li> <li class="normal">低密度と反復領域における5mCの高カバレッジ</li> <li class="normal">専任の科学者がハイタッチコミュニケーションで各プロジェクトをサポート</li> <li class="normal">包括的なサービス - バイサルファイト処理、ライブラリーの調製、シーケンシングと解析</li> </ul> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '詳細', 'info1' => '<h4>WGBS Service includes:</h4> <table style="width: 942px;"> <tbody> <tr style="text-align: left; height: 81px;"> <td style="width: 469px; height: 81px;" colspan="2"> <p><strong>QC of the genomic DNA</strong></p> <p><strong></strong></p> </td> <td style="width: 766px; height: 81px;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">Measurement of DNA concentration</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Assessment of DNA quality</span></li> </ul> </td> </tr> <tr style="height: 129px;"> <td style="width: 375px; height: 236px;" rowspan="2"> <p style="text-align: left;"><strong>Library Preparation</strong></p> <p style="text-align: left;"><strong></strong></p> </td> <td style="width: 94px; height: 129px;"> <p style="text-align: center;"><strong>WGBS</strong></p> </td> <td style="width: 766px; height: 129px;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">gDNA shearing on Bioruptor Pico (not necessary for cfDNA or FFPE)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Bisulfite conversion</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Library preparation </span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">QC of the WGBS libraries (DNA concentration, analysis of the profile)</span></li> </ul> </td> </tr> <tr style="height: 107px;"> <td style="width: 94px; text-align: center; height: 107px; background-color: #f9f9f9;"><strong>EM-seq</strong></td> <td style="width: 766px; height: 107px; background-color: #f9f9f9;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">gDNA shearing on Bioruptor Pico (not necessary for cfDNA or FFPE)</span></li> <li style="font-weight: 400;">Library preparation with Enzymatic conversion</li> <li style="font-weight: 400;">QC of EM-seq libraries (DNA concentration, analysis of the profile)</li> </ul> <span style="font-weight: 400;"></span></td> </tr> <tr style="height: 129px;"> <td style="width: 469px; height: 129px; background-color: white;" colspan="2"> <p><strong>Deep sequencing</strong></p> <p><strong></strong></p> </td> <td style="width: 766px; height: 129px; background-color: white;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;"><span>Samples are sequenced on Illumina platform, paired-end reads of 150bp length (PE150) </span> </span></li> <li style="font-weight: 400;">400M raw reads on average per samples (when pooling 6 samples/lane)</li> <li style="font-weight: 400;">Theoretical Coverage >30X for human, mouse and rat samples</li> <li style="font-weight: 400;"><span style="font-weight: 400;">Detection of >50 million CpGs with 6-9X average CpG coverage for human samples </span></li> </ul> </td> </tr> </tbody> </table> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'バイオインフォマティック解析', 'info2' => '<table style="width: 895px;"> <thead> <tr style="height: 69px;"> <th style="width: 262px; height: 69px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px; height: 69px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr style="height: 123px;"> <td style="width: 262px; height: 123px;"><strong>Standard</strong></td> <td style="width: 624px; height: 123px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr style="height: 146px;"> <td style="width: 262px; height: 146px;"><strong>Differential methylation analysis</strong></td> <td style="width: 624px; height: 146px;"> <ul> <li>Methylation level analysis</li> <li>Differentially methylated CpGs (DMCs) analysis</li> <li>Differentially methylated regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr style="height: 82px;"> <td style="width: 262px; height: 82px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px; height: 82px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr style="height: 86px;"> <td style="width: 262px; height: 86px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px; height: 86px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> <tr style="height: 86px;"> <td style="width: 262px; height: 86px;"> <p><strong>Data mining</strong></p> </td> <td style="width: 624px; height: 86px;"> <ul> <li><span>Biomarker discovery</span></li> <li><span>Determination of methylation pattern/signature that can be predictive and discriminate between different groups/conditions</span></li> </ul> </td> </tr> </tbody> </table> <h2></h2> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '', 'catalog_number' => 'G02040000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'wgbs-service', 'meta_title' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'meta_keywords' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'meta_description' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'modified' => '2022-04-11 04:55:23', 'created' => '2018-09-06 10:51:07', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '2836', 'antibody_id' => null, 'name' => 'RRBS Service (Reduced Representation Bisulfite Sequencing)', 'description' => '<p><a href="https://www.diagenode.com/en/quotes/quote?id=2836"><img alt="RRBS Service" src="https://www.diagenode.com/img/banners/b-page-promo-rrbs.png" /></a></p> <div class="extra-spaced"> <p><strong>Reduced representation bisulfite sequencing (RRBS) </strong> <span>enables </span><span>genome-s</span><span>cale </span>DNA methylation<span> analysis</span> at the single nucleotide level <span>in any vertebrate species. </span><span>The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of</span> whole genome sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent<span> biologically relevant target</span> CpG-rich regions including <span>promoters and </span>CpG islands.<span> Our RRBS service makes this technology widely available and provides high coverage (up to 7 million CpGs</span><span> detected </span><span>in human samples).</span></p> <h2>Genome-scale DNA methylation analysis</h2> <ul class="square"> <li>Robust and cost-effective solution with reliable results</li> <li>Capability to use gDNA inputs down to 25 ng</li> <li>Use of UDIs enhance sequencing results</li> <li>UMI technology eliminates PCR duplicates</li> <li>High coverage - up to 7 million CpGs detected in human samples</li> <li>Single nucleotide resolution</li> <li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li> <li>Suitable for any vertebrate species</li> </ul> <h2>Complete end-to-end service</h2> <ul class="square"> <li><span><span class="ui-provider ee cfi bsv cfj cfk cfl cfm cfn cfo cfp cfq cfr cfs cft cfu cfv cfw cfx cfy cfz cga cgb cgc cgd cge cgf cgg cgh cgi cgj cgk cgl cgm cgn cgo" dir="ltr">From DNA QC to sequencing raw data</span></span></li> <li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2</a> widely adopted</li> <li>As little as 25 ng for any vertebrate species</li> <li>Differentially methylated site analysis <span>using <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> </div> <div class="extra-spaced"> <h2></h2> </div> <p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>', 'label1' => 'Services Workflow', 'info1' => '<div class="row"> <div class="small-6 columns"> <h4>RRBS Service includes:</h4> <table style="width: 905px;"> <tbody> <tr> <td style="width: 264px;"><strong>QC of the genomic DNA</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Measurement of DNA concentration </li> <li>Assessment of DNA quality</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>MspI digestion</li> <li>Library preparation (ends preparation, adaptor ligation)</li> <li>Size selection</li> <li>Sample pooling</li> <li>Bisulfite conversion</li> <li>Library amplification and clean-up</li> <li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Deep sequencing</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li> <li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li> <li>7 million CpGs (on average) for human samples</li> <li>7-11x CpG coverage (on average) for human samples</li> </ul> </td> </tr> </tbody> </table> </div> </div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Bioinformatics Analysis', 'info2' => '<div class="row"> <div class="small-6 columns"> <table style="width: 895px;"> <thead> <tr> <th style="width: 262px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr> <td style="width: 262px;"><strong>Standard</strong></td> <td style="width: 624px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr> <td style="width: 262px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> </tbody> </table> </div> </div> <div id="ConnectiveDocSignExtentionInstalled" 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'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'rrbs-service', 'meta_title' => 'RRBS Service (Reduced Representation Bisulfite Sequencing) | Diagenode', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'RRBS enables genome-scale DNA methylation analysis at the single nucleotide level in any vertebrate species. Robust and cost-effective solution with high coverage (up to 5 million CpGs in human samples).', 'modified' => '2024-01-10 17:48:39', 'created' => '2016-09-21 16:54:44', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array([maximum depth reached]) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '105', 'position' => '1', 'parent_id' => '121', 'name' => 'DNA Methylation Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-4 large-4 columns"> <div class="panel"><center><img src="http://www.diagenode.com/img/categories/services/dna-workflow.png" alt="DNA-methylation -Diagenode" /></center> <p>The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.</p> </div> <center><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></center></div> <div class="small-12 medium-8 large-8 columns"> <p>Our Epigenomics Profiling Services helps you study DNA methylation (5mC) and methylation variants (5hmC). Our experienced scientists will guide you to the best solution according to your needs, depending on whether you want to analyze DNA methylation at genome-wide or gene specific scales. They will process the arrays or generate meaningful libraries for DNA sequencing and deliver high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</p> <h3><a href="https://www.diagenode.com/en/p/infinium-methylation-epic-array-v2-service">Infinium MethylationEPIC Array Service V2</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 930,000 CpGs detected in human samples at single nucleotide resolution</li> <li>Quantitative interrogation of CpG, non-CpG, and CHH sites</li> <li>Differential methylation analysis <span>using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> <!--<h3><a href="https://www.diagenode.com/en/p/infinium-mouse-methylation-array-service">Infinium Mouse Methylation Array Service</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 285,000 markers detected in mouse samples at single nucleotide resolution</li> <li>Suitable for common laboratory mouse strains</li> <li><span>Differential methylation analysis using our<span> </span><a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul>--> <h3><a href="https://www.diagenode.com/en/p/rrbs-service">Reduced Representation Bisulfite Sequencing (RRBS) Service</a></h3> <ul> <li>Robust and cost-effective solution with reliable results</li> <li>Unique technology enables low DNA inputs down to 25ng</li> <li>Accurate analyses with UDIs and UMIs</li> <li>Up to 7 million CpGs detected in human samples at single nucleotide resolution</li> <li>Detection of methylation patterns in CpG-rich regions across the genome including promoters and CpG islands</li> <li><span>Differential methylation analysis <span>using our</span> <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> <li>Suitable for epigenetic biomarker discovery</li> </ul> <h3><a href="https://www.diagenode.com/en/p/wgbs-service">Whole Genome Bisulfite Sequencing (WGBS) or Enzymatic Methylation(EM-seq) Service</a></h3> <ul> <li>Very powerful solution for genome-wide biomarker discovery using bisulfite or enzymatic conversion</li> <li><span>Evaluation of methylation status of nearly every CpG sites of the entire genome </span>at single nucleotide resolution</li> <li>Detection of global methylation patterns including in low CpG-density regions and outside of CpG islands</li> <li>Identification of regions or even loci with differential methylation levels between groups using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></li> </ul> <h3><a href="https://www.diagenode.com/en/p/targeted-dna-methylation-service">Targeted Bisulfite Sequencing Service</a></h3> <ul> <li>Focus on D<span>NA methylation patterning of selective regions with single nucleotide resolution</span></li> <li><span>Choose between two methodologies depending on the size of the region of interest: PCR enrichment or hybridization capture</span></li> <li><span>Enhanced accuracy, sensitivity and specificity while reducing overall cost</span></li> <li><span>Allowing deep sequencing at high coverage levels for rare variant identification</span></li> </ul> <!--<h3><a href="../p/medip-seq-service">Methylated DNA IP Sequencing</a></h3> <ul> <li>Immunoprecipitation assay (antibody-based approach) to study 5-mC genome-wide</li> <li>Resolution of 100-500 bp</li> </ul>--> <h3><a href="../p/human-methylome-service">Human Methylome</a></h3> <h3>Watch our webinar:</h3> <p>Cost-Effective Genome Wide DNA Methylation Analysis using Twist Hybrid-Capture Methylome Panel</p> <a class="popup-youtube" href="#webinar"><img src="https://www.diagenode.com/img/webinar/eshg-miniature.png" class="webinar" alt="Webinar series: Genome Wide DNA Methylation using Twist Hybrid-Capture Methylome Panel" /></a> <p></p> <ul> <li>NGS service assay for comprehensive DNA methylation profiling in human samples from solid or liquid biopsy</li> <li>Optimal biomarker discovery tool for cancer, neurodegenerative, cardiovascular and metabolic diseases</li> <li>High coverage for more than 3.89 million methylation sites (~9 million CpGs at single strand level)</li> </ul> </div> </div>', 'no_promo' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'dna-methylation-profiling-services', 'cookies_tag_id' => '3', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'Diagenode DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.', 'meta_title' => 'DNA Methylation Profiling Services EPIC array, RRBS, WGBS, MeDIP/hMeDIP-seq and Targeted DNA methylation analysis | Diagenode', 'modified' => '2023-10-04 16:04:25', 'created' => '2015-11-23 14:29:35', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '121', 'position' => '9', 'parent_id' => null, 'name' => 'Epigenomics Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-10 large-10 columns text-left"> <p>Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis. </p> </div> <div class="small-12 medium-2 large-2 columns text-left"><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></div> </div> <center><img alt="DNA-RNA-Chromatin-Diagenode" src="https://www.diagenode.com/img/categories/services/chromatin-dna-rna.png" /></center> <div class="row"> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #099f92;"> <h3><span class="green">Chromatin</span> analysis</h3> <ul> <li>ChIP-seq analysis Histone modification</li> <li>Promoter analysis</li> <li>Enhancer analysis</li> <li>ChIP-seq analysis Transcription factor </li> <li>(sc)ATAC-seq Chromatin accessibility</li> <li>ChIP-seq Cas9 off-target</li> <li>Customized NGS service</li> </ul> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/chip-seq-service">Learn more</a></p> </div> </div> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #474546;"> <h3><span class="darkgrey">DNA methylation</span> analysis</h3> <ul> <li>Reduced representation bisulfite sequencing (RRBS)</li> <li>Whole genome bisulfite sequencing (WGBS)</li> <li>Targeted methylation</li> <li>MeDIP-seq/hMeDIP-seq</li> <li>Infinium MethylationEPIC array </li> </ul> <p></p> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/dna-methylation-profiling-services">Learn more</a></p> </div> </div> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #b11e33;"> <h3><span class="diacol"><g class="gr_ gr_44 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="44" data-gr-id="44">RNA-seq</g></span> analysis</h3> <ul> <li><span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;">Gene expression profiling</span></li> <li>mRNA<span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;"> analysis</span></li> <li>Small non-coding RNA analysis</li> <li><b></b>Whole transcriptome analysis<br /><br /><br /><br /><br /></li> </ul> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/rna-seq-category">Learn more</a></p> </div> </div> </div> <h2 style="text-align: center;"><a class="details radius small button" href="https://www.diagenode.com/en/documents/services-flyer">DOWNLOAD OUR FLYER</a></h2> <h2>Why Diagenode</h2> <ul> <li><strong>Expertise and trust</strong>: Recognized epigenetics leader, official partner of <a href="http://www.blueprint-epigenome.eu/"><g class="gr_ gr_45 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="45" data-gr-id="45">BLUEPRINT</g></a>, <a href="http://ihec-epigenomes.org/">IHEC</a> <g class="gr_ gr_46 gr-alert gr_gramm gr_inline_cards gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="46" data-gr-id="46">and</g> <a href="https://www.faang.org/">FAANG</a></li> <li><strong>Innovative technology</strong>: Utilization of the signature Bioruptor<sup>®</sup> sonication device for optimal chromatin and DNA shearing and the IP-Star<sup>®</sup> Automation device give reproducible and reliable optimization and results</li> <li><strong>Quality</strong>: Multiple QC steps in all workflows and validated antibodies plus reagents deliver superior data</li> <li><strong>Flexibility</strong>: Extensive range of sample species and sample origins</li> <li><strong>Experience</strong> in epigenomics profiling for a wide range of applications and fields of interest</li> </ul> <div class="extra-spaced"> <ul class="small-block-grid-2 medium-block-grid-3 large-block-grid-3"> <li> <div class="block-distri"><span class="label-green" style="margin-bottom: 16px; 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-3022 #state-3022").parent().parent().show(); } else { $("#Quote-3022 #state-3022").parent().parent().hide(); $("#Quote-3022 #state-3022").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3022" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="data-mining-service" data-reveal-id="quoteModal-3022" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">データマイニングサービス</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/wgbs-service"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">G02040000</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2989" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>our epigenomics services</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=2989" id="Quote-2989" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2989" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RGxyN3VUNERGenBPUExVK3k0L0Ivdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="wgbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Service Information</h2> </div> <div class="small-12 large-12 columns"> <h4>Which services are you interested in?</h4> </div> <div class="small-12 large-12 columns"> <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium MethylationEPIC Array v2" id="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2" /><label for="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2">Infinium MethylationEPIC Array v2</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Human Methylome" id="QuoteEpigenomicsServiceHumanMethylome" /><label for="QuoteEpigenomicsServiceHumanMethylome">Human Methylome</label></div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-3 columns"> <span class="prefix">Sample species</span> </div> <div class="small-12 medium-12 large-9 columns"> <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/> </div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-6 columns"> <span class="prefix">Total number of samples (including replicates)</span> </div> <div class="small-12 medium-12 large-6 columns"> <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/> </div> </div> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2989"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option value="AW">Aruba</option> <option value="AU">Australia</option> <option value="AT">Austria</option> <option value="AZ">Azerbaijan</option> <option value="BS">Bahamas</option> <option value="BH">Bahrain</option> <option value="BD">Bangladesh</option> <option value="BB">Barbados</option> <option value="BY">Belarus</option> <option value="BE">Belgium</option> <option value="BZ">Belize</option> <option value="BJ">Benin</option> <option value="BM">Bermuda</option> <option value="BT">Bhutan</option> <option value="BO">Bolivia</option> <option value="BQ">Bonaire, Sint Eustatius and Saba</option> <option value="BA">Bosnia and Herzegovina</option> <option value="BW">Botswana</option> <option value="BV">Bouvet Island</option> <option value="BR">Brazil</option> <option value="IO">British Indian Ocean Territory</option> <option value="BN">Brunei Darussalam</option> <option value="BG">Bulgaria</option> <option value="BF">Burkina Faso</option> <option value="BI">Burundi</option> <option value="KH">Cambodia</option> <option value="CM">Cameroon</option> <option value="CA">Canada</option> <option value="CV">Cape Verde</option> <option value="KY">Cayman Islands</option> <option value="CF">Central African Republic</option> <option value="TD">Chad</option> <option value="CL">Chile</option> <option value="CN">China</option> <option value="CX">Christmas Island</option> <option value="CC">Cocos (Keeling) Islands</option> <option value="CO">Colombia</option> <option value="KM">Comoros</option> <option value="CG">Congo</option> <option value="CD">Congo, The Democratic Republic of the</option> <option value="CK">Cook Islands</option> <option value="CR">Costa Rica</option> <option value="CI">Côte d'Ivoire</option> <option value="HR">Croatia</option> <option value="CU">Cuba</option> <option value="CW">Curaçao</option> <option value="CY">Cyprus</option> <option value="CZ">Czech Republic</option> <option value="DK">Denmark</option> <option value="DJ">Djibouti</option> <option value="DM">Dominica</option> <option value="DO">Dominican Republic</option> <option value="EC">Ecuador</option> <option value="EG">Egypt</option> <option value="SV">El Salvador</option> <option value="GQ">Equatorial Guinea</option> <option value="ER">Eritrea</option> <option value="EE">Estonia</option> <option value="ET">Ethiopia</option> <option value="FK">Falkland Islands (Malvinas)</option> <option value="FO">Faroe Islands</option> <option value="FJ">Fiji</option> <option value="FI">Finland</option> <option value="FR">France</option> <option value="GF">French Guiana</option> <option value="PF">French Polynesia</option> <option value="TF">French Southern Territories</option> <option value="GA">Gabon</option> <option value="GM">Gambia</option> <option value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option value="GD">Grenada</option> <option value="GP">Guadeloupe</option> <option value="GU">Guam</option> <option value="GT">Guatemala</option> <option value="GG">Guernsey</option> <option value="GN">Guinea</option> <option value="GW">Guinea-Bissau</option> <option value="GY">Guyana</option> <option value="HT">Haiti</option> <option value="HM">Heard Island and McDonald Islands</option> <option value="VA">Holy See (Vatican City State)</option> <option value="HN">Honduras</option> <option value="HK">Hong Kong</option> <option value="HU">Hungary</option> <option value="IS">Iceland</option> <option value="IN">India</option> <option value="ID">Indonesia</option> <option value="IR">Iran, Islamic Republic of</option> <option value="IQ">Iraq</option> <option value="IE">Ireland</option> <option value="IM">Isle of Man</option> <option value="IL">Israel</option> <option value="IT">Italy</option> <option value="JM">Jamaica</option> <option value="JP">Japan</option> <option value="JE">Jersey</option> <option value="JO">Jordan</option> <option value="KZ">Kazakhstan</option> <option value="KE">Kenya</option> <option value="KI">Kiribati</option> <option value="KP">Korea, Democratic People's Republic of</option> <option value="KR">Korea, Republic of</option> <option value="KW">Kuwait</option> <option value="KG">Kyrgyzstan</option> <option value="LA">Lao People's Democratic Republic</option> <option value="LV">Latvia</option> <option value="LB">Lebanon</option> <option value="LS">Lesotho</option> <option value="LR">Liberia</option> <option value="LY">Libya</option> <option value="LI">Liechtenstein</option> <option value="LT">Lithuania</option> <option value="LU">Luxembourg</option> <option value="MO">Macao</option> <option value="MK">Macedonia, Republic of</option> <option value="MG">Madagascar</option> <option value="MW">Malawi</option> <option value="MY">Malaysia</option> <option value="MV">Maldives</option> <option value="ML">Mali</option> <option value="MT">Malta</option> <option value="MH">Marshall Islands</option> <option value="MQ">Martinique</option> <option value="MR">Mauritania</option> <option value="MU">Mauritius</option> <option value="YT">Mayotte</option> <option value="MX">Mexico</option> <option value="FM">Micronesia, Federated States of</option> <option value="MD">Moldova</option> <option value="MC">Monaco</option> <option value="MN">Mongolia</option> <option value="ME">Montenegro</option> <option value="MS">Montserrat</option> <option value="MA">Morocco</option> <option value="MZ">Mozambique</option> <option value="MM">Myanmar</option> <option value="NA">Namibia</option> <option value="NR">Nauru</option> <option value="NP">Nepal</option> <option value="NL">Netherlands</option> <option value="NC">New Caledonia</option> <option value="NZ">New Zealand</option> <option value="NI">Nicaragua</option> <option value="NE">Niger</option> <option value="NG">Nigeria</option> <option value="NU">Niue</option> <option value="NF">Norfolk Island</option> <option 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Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=2836" id="Quote-2836" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2836" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RGxyN3VUNERGenBPUExVK3k0L0Ivdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="rrbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Service Information</h2> </div> <div class="small-12 large-12 columns"> <h4>Which services are you interested in?</h4> </div> <div class="small-12 large-12 columns"> <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium MethylationEPIC Array v2" id="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2" /><label for="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2">Infinium MethylationEPIC Array v2</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Human Methylome" id="QuoteEpigenomicsServiceHumanMethylome" /><label for="QuoteEpigenomicsServiceHumanMethylome">Human Methylome</label></div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-3 columns"> <span class="prefix">Sample species</span> </div> <div class="small-12 medium-12 large-9 columns"> <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/> </div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-6 columns"> <span class="prefix">Total number of samples (including replicates)</span> </div> <div class="small-12 medium-12 large-6 columns"> <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/> </div> </div> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2836"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option 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value="DM">Dominica</option> <option value="DO">Dominican Republic</option> <option value="EC">Ecuador</option> <option value="EG">Egypt</option> <option value="SV">El Salvador</option> <option value="GQ">Equatorial Guinea</option> <option value="ER">Eritrea</option> <option value="EE">Estonia</option> <option value="ET">Ethiopia</option> <option value="FK">Falkland Islands (Malvinas)</option> <option value="FO">Faroe Islands</option> <option value="FJ">Fiji</option> <option value="FI">Finland</option> <option value="FR">France</option> <option value="GF">French Guiana</option> <option value="PF">French Polynesia</option> <option value="TF">French Southern Territories</option> <option value="GA">Gabon</option> <option value="GM">Gambia</option> <option value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option 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#state-2836").parent().parent().show(); } else { $("#Quote-2836 #state-2836").parent().parent().hide(); $("#Quote-2836 #state-2836").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2836" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="rrbs-service" data-reveal-id="quoteModal-2836" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">RRBS受託サービス (Reduced Representation Bisulfite Se...</h6> </div> </div> </li> ' $related = array( 'id' => '2836', 'antibody_id' => null, 'name' => 'RRBS Service (Reduced Representation Bisulfite Sequencing)', 'description' => '<p><a href="https://www.diagenode.com/en/quotes/quote?id=2836"><img alt="RRBS Service" src="https://www.diagenode.com/img/banners/b-page-promo-rrbs.png" /></a></p> <div class="extra-spaced"> <p><strong>Reduced representation bisulfite sequencing (RRBS) </strong> <span>enables </span><span>genome-s</span><span>cale </span>DNA methylation<span> analysis</span> at the single nucleotide level <span>in any vertebrate species. </span><span>The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of</span> whole genome sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent<span> biologically relevant target</span> CpG-rich regions including <span>promoters and </span>CpG islands.<span> Our RRBS service makes this technology widely available and provides high coverage (up to 7 million CpGs</span><span> detected </span><span>in human samples).</span></p> <h2>Genome-scale DNA methylation analysis</h2> <ul class="square"> <li>Robust and cost-effective solution with reliable results</li> <li>Capability to use gDNA inputs down to 25 ng</li> <li>Use of UDIs enhance sequencing results</li> <li>UMI technology eliminates PCR duplicates</li> <li>High coverage - up to 7 million CpGs detected in human samples</li> <li>Single nucleotide resolution</li> <li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li> <li>Suitable for any vertebrate species</li> </ul> <h2>Complete end-to-end service</h2> <ul class="square"> <li><span><span class="ui-provider ee cfi bsv cfj cfk cfl cfm cfn cfo cfp cfq cfr cfs cft cfu cfv cfw cfx cfy cfz cga cgb cgc cgd cge cgf cgg cgh cgi cgj cgk cgl cgm cgn cgo" dir="ltr">From DNA QC to sequencing raw data</span></span></li> <li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2</a> widely adopted</li> <li>As little as 25 ng for any vertebrate species</li> <li>Differentially methylated site analysis <span>using <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> </div> <div class="extra-spaced"> <h2></h2> </div> <p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>', 'label1' => 'Services Workflow', 'info1' => '<div class="row"> <div class="small-6 columns"> <h4>RRBS Service includes:</h4> <table style="width: 905px;"> <tbody> <tr> <td style="width: 264px;"><strong>QC of the genomic DNA</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Measurement of DNA concentration </li> <li>Assessment of DNA quality</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>MspI digestion</li> <li>Library preparation (ends preparation, adaptor ligation)</li> <li>Size selection</li> <li>Sample pooling</li> <li>Bisulfite conversion</li> <li>Library amplification and clean-up</li> <li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Deep sequencing</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li> <li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li> <li>7 million CpGs (on average) for human samples</li> <li>7-11x CpG coverage (on average) for human samples</li> </ul> </td> </tr> </tbody> </table> </div> </div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Bioinformatics Analysis', 'info2' => '<div class="row"> <div class="small-6 columns"> <table style="width: 895px;"> <thead> <tr> <th style="width: 262px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr> <td style="width: 262px;"><strong>Standard</strong></td> <td style="width: 624px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr> <td style="width: 262px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> </tbody> </table> </div> </div> <div id="ConnectiveDocSignExtentionInstalled" 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line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Targeted DNA methylation 受託サービスは、ゲノム全体の既知の正確な位置でのシトシンメチル化の定量的研究を可能にします。関心領域の大きさに応じて、バイサルファイトパイロシーケンシング、バイサルファイトアンプリコンシーケンシング（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">BSAS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）、およびプローブによるカスタムキャプチャーなどの数多くの方法論を用いることが可能です。これらの技術は、単一ヌクレオチド分解能で標的領域のメチル化状態を定量的に検出するために、<em>バイサルファイト変換</em>とその後のシーケンシングに共通の方法論を共有しています。</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; color: #222222;"><o:p></o:p></span></p>', 'label1' => '詳細', 'info1' => '<p style="text-align: left;">Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:</p> <table style="width: 941px;"> <thead> <tr style="height: 69px;"> <th style="width: 218px; height: 69px;"> <h4><strong>Workflow</strong></h4> </th> <th style="width: 402px; 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line-height: 150%;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Targeted DNA methylation 受託サービスは、ゲノム全体の既知の正確な位置でのシトシンメチル化の定量的研究を可能にします。関心領域の大きさに応じて、バイサルファイトパイロシーケンシング、バイサルファイトアンプリコンシーケンシング（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">BSAS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）、およびプローブによるカスタムキャプチャーなどの数多くの方法論を用いることが可能です。これらの技術は、単一ヌクレオチド分解能で標的領域のメチル化状態を定量的に検出するために、<em>バイサルファイト変換</em>とその後のシーケンシングに共通の方法論を共有しています。</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; color: #222222;"><o:p></o:p></span></p>', 'label1' => '詳細', 'info1' => '<p style="text-align: left;">Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:</p> <table style="width: 941px;"> <thead> <tr style="height: 69px;"> <th style="width: 218px; 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encrypted-media" allowfullscreen="allowfullscreen"></iframe></center> <p></p> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <blockquote style="padding-bottom: 0;"><span class="label-green" style="margin-bottom: 16px; margin-left: -22px; font-size: 22px;">WHITE PAPERS</span> <div id="portal" class="main-portal"> <div class="portal-inner"><nav class="portal-nav" style="text-align: left;"> <ul data-tab="" class="tips-menu"> <li><a href="#panel1" class="tips portal button">Smokers vs non-smokers </a></li> <li><a href="#panel2" class="tips portal button">Breast cancer</a></li> </ul> </nav></div> </div> <div class="tabs-content"> <div class="content active" id="panel1"> <h3 style="margin-top: 0;">Powerful new insights with epigenetic data mining.<br /> A study to distinguish smokers from non-smokers using just one droplet of blood</h3> <p>Next generation sequencing in combination with sophisticated bioinformatics technologies for genomic, transcriptomic and epigeneomic analyses have enormous potential to establish new biomarkers for disease diagnostics, enabling true precision medicine. Analyses of liquid biopsies to measure thousands of different data points simultaneously in easily accessible body fluids (e.g. blood, urine, and saliva) are extremely promising for such biomarker studies.<br />(...)</p> <a href="https://www.diagenode.com/en/pages/form-bioinfo" class="alert small button" target="_blank">Read more</a></div> <div class="content" id="panel2"> <h3 style="margin-top: 0;">Data mining on DNA methylation data in cancer samples<br />Distinguishing normal from breast cancer tissue</h3> <p>Breast cancer is the most commonly occurring cancer in women and the second most common cancer overall.</p> <p>One important aspect of cancer tissues it that they differ from normal tissues in their epigenetic make up, especially in the DNA methylation pattern. In normal cells methylation assures the proper regulation of gene expression and stable gene silencing. DNA methylation is associated with histone modifications, and the interplay of these epigenetic modifications is crucial to regulate the functioning of the genome by changing chromatin architecture.<br />(...)</p> <a href="https://www.diagenode.com/en/pages/form-bioinfo?app_note=23" class="alert small button" target="_blank">Read more</a></div> </div> </blockquote> </div> </div> <p>Diagenode's new data mining service utilizes methods at the frontier of machine learning, statistics, and database systems. This enhanced service supports new discoveries that were previously not possible by analyzing patterns in large data sets to give informative new insights.</p> <p>If you have data from patient cohorts, single cell analyses or any other heterogeneous scenarios, our service team provides enhanced support with optimal data analysis using our latest data mining capabilities. Specifically, our team applies machine learning technologies to find previously undiscovered or unobvious relationships within and across datasets. This advanced technology allows discovery of informative features from mass data, essentially “finding a needle in a haystack.”</p> <p>Diagenode utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data. Our goal is to build strong classifiers that separate data into two or more classes or groups depending on associated data.</p> <p class="extra-spaced">Different and multiple -omics data classes can be mined simultaneously. Integration with phenotypic and/or clinical data is also possible. We offer data mining services for several data classes including:</p> <table class="extra-spaced"> <tbody> <tr> <td><strong>Epigenetic data</strong></td> <td><strong>Transcriptomic data</strong></td> </tr> <tr> <td> <p>DNA Methylation (RRBS, WGBS, EPIC arrays)</p> <p>ChIP-sequencing</p> <p>ATAC-seq</p> </td> <td> <p>mRNA-sequencing</p> <p>Small and long non coding RNA</p> <p>Single-cell RNA-sequencing</p> </td> </tr> </tbody> </table> <p></p> <p><strong>Biological Interpretation</strong></p> <p class="extra-spaced">Machine learning classifiers also mirror the underlying biological differences between classes and are used to uncover the molecular processes at work. In order to achieve this, we offer biological interpretation services and pathway mining analyses for your data.</p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Data mining modules ', 'info1' => '<ol> <li><strong>Feasibility study</strong></li> </ol> <ul> <li>Assessment of data characteristics and <span></span>applicability of different machine learning (ML)</li> <li>Prototypic analyses:</li> <li>Initial feasibility report</li> </ul> <p></p> <ol start="2"> <li><strong>Data Mining</strong></li> </ol> <ul> <li>Machine learning on data</li> <li>Data evaluation and validation</li> <li>Report generation</li> </ul> <p> </p> <ol start="3"> <li><strong>Data Interpretation</strong></li> </ol> <ul> <li>Integration of background knowledge</li> <li>Functional interpretation / pathway mining</li> <li>Scientific reporting</li> </ul> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'Integration with wet lab services and bioinformatics', 'info2' => '<p>A clear advantage of <strong>Diagenode’s data mining services</strong> is the close connection with<span> </span>other service offerings like <strong>wet lab analysis services</strong> and <strong>bioinformatics services</strong>. You can retrieve a full service package from a single source.</p> <p><a href="https://www.diagenode.com/en/categories/Services">Read about our wetlab services</a></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => 'Custom', 'catalog_number' => 'G02100000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'data-mining-service', 'meta_title' => 'Bioinformatics Data Mining Service | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Diagenode Data Mining Services utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data.', 'modified' => '2020-12-21 08:05:15', 'created' => '2019-04-08 16:43:26', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2989', 'antibody_id' => null, 'name' => 'WGBS (Whole Genome Bisulfite Sequencing) and EM-seq (Enzymatic Methylation)受託サービス ', 'description' => '<p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Whole-genome bisulfite sequencing（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">WGBS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）は、</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">DNA</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">メチル化プロファイリングの中でも最も包括的な次世代シーケンシングであり、ゲノム全体で</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">5-mC</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">の単一塩基分解能を可能にします。</span></p> <p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">同じ場所での変換されていないシトシンと変換されたシトシンの割合を比較することにより、メチル化レベルが決定されます。</span></p> <p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;"></span></p> <pre class="tw-data-text tw-text-large tw-ta" data-placeholder="Translation" id="tw-target-text" dir="ltr"><span class="Y2IQFc" lang="ja"></span><span lang="EN" style="font-family: 'Arial Unicode MS'; color: #b21329;">DNA</span><span lang="FR" style="font-family: 'Arial Unicode MS'; color: #b21329;">メチル化研究において最も高いカバレッジ</span></pre> <ul> <li class="normal"><span>ゲノム全体のDNAメチル化と単一塩基分解能スクリーニング</span></li> <li class="normal">低密度と反復領域における5mCの高カバレッジ</li> <li class="normal">専任の科学者がハイタッチコミュニケーションで各プロジェクトをサポート</li> <li class="normal">包括的なサービス - バイサルファイト処理、ライブラリーの調製、シーケンシングと解析</li> </ul> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '詳細', 'info1' => '<h4>WGBS Service includes:</h4> <table style="width: 942px;"> <tbody> <tr style="text-align: left; height: 81px;"> <td style="width: 469px; height: 81px;" colspan="2"> <p><strong>QC of the genomic DNA</strong></p> <p><strong></strong></p> </td> <td style="width: 766px; height: 81px;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">Measurement of DNA concentration</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Assessment of DNA quality</span></li> </ul> </td> </tr> <tr style="height: 129px;"> <td style="width: 375px; height: 236px;" rowspan="2"> <p style="text-align: left;"><strong>Library Preparation</strong></p> <p style="text-align: left;"><strong></strong></p> </td> <td style="width: 94px; height: 129px;"> <p style="text-align: center;"><strong>WGBS</strong></p> </td> <td style="width: 766px; height: 129px;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">gDNA shearing on Bioruptor Pico (not necessary for cfDNA or FFPE)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Bisulfite conversion</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Library preparation </span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">QC of the WGBS libraries (DNA concentration, analysis of the profile)</span></li> </ul> </td> </tr> <tr style="height: 107px;"> <td style="width: 94px; text-align: center; height: 107px; background-color: #f9f9f9;"><strong>EM-seq</strong></td> <td style="width: 766px; height: 107px; background-color: #f9f9f9;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">gDNA shearing on Bioruptor Pico (not necessary for cfDNA or FFPE)</span></li> <li style="font-weight: 400;">Library preparation with Enzymatic conversion</li> <li style="font-weight: 400;">QC of EM-seq libraries (DNA concentration, analysis of the profile)</li> </ul> <span style="font-weight: 400;"></span></td> </tr> <tr style="height: 129px;"> <td style="width: 469px; height: 129px; background-color: white;" colspan="2"> <p><strong>Deep sequencing</strong></p> <p><strong></strong></p> </td> <td style="width: 766px; height: 129px; background-color: white;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;"><span>Samples are sequenced on Illumina platform, paired-end reads of 150bp length (PE150) </span> </span></li> <li style="font-weight: 400;">400M raw reads on average per samples (when pooling 6 samples/lane)</li> <li style="font-weight: 400;">Theoretical Coverage >30X for human, mouse and rat samples</li> <li style="font-weight: 400;"><span style="font-weight: 400;">Detection of >50 million CpGs with 6-9X average CpG coverage for human samples </span></li> </ul> </td> </tr> </tbody> </table> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'バイオインフォマティック解析', 'info2' => '<table style="width: 895px;"> <thead> <tr style="height: 69px;"> <th style="width: 262px; height: 69px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px; height: 69px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr style="height: 123px;"> <td style="width: 262px; height: 123px;"><strong>Standard</strong></td> <td style="width: 624px; height: 123px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr style="height: 146px;"> <td style="width: 262px; height: 146px;"><strong>Differential methylation analysis</strong></td> <td style="width: 624px; height: 146px;"> <ul> <li>Methylation level analysis</li> <li>Differentially methylated CpGs (DMCs) analysis</li> <li>Differentially methylated regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr style="height: 82px;"> <td style="width: 262px; height: 82px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px; height: 82px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr style="height: 86px;"> <td style="width: 262px; height: 86px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px; height: 86px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> <tr style="height: 86px;"> <td style="width: 262px; height: 86px;"> <p><strong>Data mining</strong></p> </td> <td style="width: 624px; height: 86px;"> <ul> <li><span>Biomarker discovery</span></li> <li><span>Determination of methylation pattern/signature that can be predictive and discriminate between different groups/conditions</span></li> </ul> </td> </tr> </tbody> </table> <h2></h2> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '', 'catalog_number' => 'G02040000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'wgbs-service', 'meta_title' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'meta_keywords' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'meta_description' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'modified' => '2022-04-11 04:55:23', 'created' => '2018-09-06 10:51:07', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '2836', 'antibody_id' => null, 'name' => 'RRBS Service (Reduced Representation Bisulfite Sequencing)', 'description' => '<p><a href="https://www.diagenode.com/en/quotes/quote?id=2836"><img alt="RRBS Service" src="https://www.diagenode.com/img/banners/b-page-promo-rrbs.png" /></a></p> <div class="extra-spaced"> <p><strong>Reduced representation bisulfite sequencing (RRBS) </strong> <span>enables </span><span>genome-s</span><span>cale </span>DNA methylation<span> analysis</span> at the single nucleotide level <span>in any vertebrate species. </span><span>The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of</span> whole genome sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent<span> biologically relevant target</span> CpG-rich regions including <span>promoters and </span>CpG islands.<span> Our RRBS service makes this technology widely available and provides high coverage (up to 7 million CpGs</span><span> detected </span><span>in human samples).</span></p> <h2>Genome-scale DNA methylation analysis</h2> <ul class="square"> <li>Robust and cost-effective solution with reliable results</li> <li>Capability to use gDNA inputs down to 25 ng</li> <li>Use of UDIs enhance sequencing results</li> <li>UMI technology eliminates PCR duplicates</li> <li>High coverage - up to 7 million CpGs detected in human samples</li> <li>Single nucleotide resolution</li> <li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li> <li>Suitable for any vertebrate species</li> </ul> <h2>Complete end-to-end service</h2> <ul class="square"> <li><span><span class="ui-provider ee cfi bsv cfj cfk cfl cfm cfn cfo cfp cfq cfr cfs cft cfu cfv cfw cfx cfy cfz cga cgb cgc cgd cge cgf cgg cgh cgi cgj cgk cgl cgm cgn cgo" dir="ltr">From DNA QC to sequencing raw data</span></span></li> <li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2</a> widely adopted</li> <li>As little as 25 ng for any vertebrate species</li> <li>Differentially methylated site analysis <span>using <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> </div> <div class="extra-spaced"> <h2></h2> </div> <p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>', 'label1' => 'Services Workflow', 'info1' => '<div class="row"> <div class="small-6 columns"> <h4>RRBS Service includes:</h4> <table style="width: 905px;"> <tbody> <tr> <td style="width: 264px;"><strong>QC of the genomic DNA</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Measurement of DNA concentration </li> <li>Assessment of DNA quality</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>MspI digestion</li> <li>Library preparation (ends preparation, adaptor ligation)</li> <li>Size selection</li> <li>Sample pooling</li> <li>Bisulfite conversion</li> <li>Library amplification and clean-up</li> <li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Deep sequencing</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li> <li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li> <li>7 million CpGs (on average) for human samples</li> <li>7-11x CpG coverage (on average) for human samples</li> </ul> </td> </tr> </tbody> </table> </div> </div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Bioinformatics Analysis', 'info2' => '<div class="row"> <div class="small-6 columns"> <table style="width: 895px;"> <thead> <tr> <th style="width: 262px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr> <td style="width: 262px;"><strong>Standard</strong></td> <td style="width: 624px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr> <td style="width: 262px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> </tbody> </table> </div> </div> <div id="ConnectiveDocSignExtentionInstalled" 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Robust and cost-effective solution with high coverage (up to 5 million CpGs in human samples).', 'modified' => '2024-01-10 17:48:39', 'created' => '2016-09-21 16:54:44', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array([maximum depth reached]) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '105', 'position' => '1', 'parent_id' => '121', 'name' => 'DNA Methylation Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-4 large-4 columns"> <div class="panel"><center><img src="http://www.diagenode.com/img/categories/services/dna-workflow.png" alt="DNA-methylation -Diagenode" /></center> <p>The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.</p> </div> <center><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></center></div> <div class="small-12 medium-8 large-8 columns"> <p>Our Epigenomics Profiling Services helps you study DNA methylation (5mC) and methylation variants (5hmC). Our experienced scientists will guide you to the best solution according to your needs, depending on whether you want to analyze DNA methylation at genome-wide or gene specific scales. They will process the arrays or generate meaningful libraries for DNA sequencing and deliver high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</p> <h3><a href="https://www.diagenode.com/en/p/infinium-methylation-epic-array-v2-service">Infinium MethylationEPIC Array Service V2</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 930,000 CpGs detected in human samples at single nucleotide resolution</li> <li>Quantitative interrogation of CpG, non-CpG, and CHH sites</li> <li>Differential methylation analysis <span>using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> <!--<h3><a href="https://www.diagenode.com/en/p/infinium-mouse-methylation-array-service">Infinium Mouse Methylation Array Service</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 285,000 markers detected in mouse samples at single nucleotide resolution</li> <li>Suitable for common laboratory mouse strains</li> <li><span>Differential methylation analysis using our<span> </span><a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul>--> <h3><a href="https://www.diagenode.com/en/p/rrbs-service">Reduced Representation Bisulfite Sequencing (RRBS) Service</a></h3> <ul> <li>Robust and cost-effective solution with reliable results</li> <li>Unique technology enables low DNA inputs down to 25ng</li> <li>Accurate analyses with UDIs and UMIs</li> <li>Up to 7 million CpGs detected in human samples at single nucleotide resolution</li> <li>Detection of methylation patterns in CpG-rich regions across the genome including promoters and CpG islands</li> <li><span>Differential methylation analysis <span>using our</span> <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> <li>Suitable for epigenetic biomarker discovery</li> </ul> <h3><a href="https://www.diagenode.com/en/p/wgbs-service">Whole Genome Bisulfite Sequencing (WGBS) or Enzymatic Methylation(EM-seq) Service</a></h3> <ul> <li>Very powerful solution for genome-wide biomarker discovery using bisulfite or enzymatic conversion</li> <li><span>Evaluation of methylation status of nearly every CpG sites of the entire genome </span>at single nucleotide resolution</li> <li>Detection of global methylation patterns including in low CpG-density regions and outside of CpG islands</li> <li>Identification of regions or even loci with differential methylation levels between groups using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></li> </ul> <h3><a href="https://www.diagenode.com/en/p/targeted-dna-methylation-service">Targeted Bisulfite Sequencing Service</a></h3> <ul> <li>Focus on D<span>NA methylation patterning of selective regions with single nucleotide resolution</span></li> <li><span>Choose between two 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comprehensive DNA methylation profiling in human samples from solid or liquid biopsy</li> <li>Optimal biomarker discovery tool for cancer, neurodegenerative, cardiovascular and metabolic diseases</li> <li>High coverage for more than 3.89 million methylation sites (~9 million CpGs at single strand level)</li> </ul> </div> </div>', 'no_promo' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'dna-methylation-profiling-services', 'cookies_tag_id' => '3', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'Diagenode DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.', 'meta_title' => 'DNA Methylation Profiling Services EPIC array, RRBS, WGBS, MeDIP/hMeDIP-seq and Targeted DNA methylation analysis | Diagenode', 'modified' => '2023-10-04 16:04:25', 'created' => '2015-11-23 14:29:35', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '121', 'position' => '9', 'parent_id' => null, 'name' => 'Epigenomics Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-10 large-10 columns text-left"> <p>Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis. </p> </div> <div class="small-12 medium-2 large-2 columns text-left"><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></div> </div> <center><img alt="DNA-RNA-Chromatin-Diagenode" src="https://www.diagenode.com/img/categories/services/chromatin-dna-rna.png" /></center> <div class="row"> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #099f92;"> <h3><span class="green">Chromatin</span> analysis</h3> <ul> <li>ChIP-seq analysis Histone modification</li> <li>Promoter analysis</li> <li>Enhancer analysis</li> <li>ChIP-seq analysis Transcription factor </li> <li>(sc)ATAC-seq Chromatin accessibility</li> <li>ChIP-seq Cas9 off-target</li> <li>Customized NGS service</li> </ul> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/chip-seq-service">Learn more</a></p> </div> </div> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #474546;"> <h3><span class="darkgrey">DNA methylation</span> analysis</h3> <ul> <li>Reduced representation bisulfite sequencing (RRBS)</li> <li>Whole genome bisulfite sequencing (WGBS)</li> <li>Targeted methylation</li> <li>MeDIP-seq/hMeDIP-seq</li> <li>Infinium MethylationEPIC array </li> </ul> <p></p> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/dna-methylation-profiling-services">Learn more</a></p> </div> </div> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #b11e33;"> <h3><span class="diacol"><g class="gr_ gr_44 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="44" data-gr-id="44">RNA-seq</g></span> analysis</h3> <ul> <li><span style="display: inline !important; 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id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha66296bb0109ac"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-3022" class="alert button expand" form="Quote-3022" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">G02040000</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2989" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>our epigenomics services</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=2989" id="Quote-2989" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2989" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RGxyN3VUNERGenBPUExVK3k0L0Ivdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="wgbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Service Information</h2> </div> <div class="small-12 large-12 columns"> <h4>Which services are you interested in?</h4> </div> <div class="small-12 large-12 columns"> <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium MethylationEPIC Array v2" id="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2" /><label for="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2">Infinium MethylationEPIC Array v2</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Human Methylome" id="QuoteEpigenomicsServiceHumanMethylome" /><label for="QuoteEpigenomicsServiceHumanMethylome">Human Methylome</label></div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-3 columns"> <span class="prefix">Sample species</span> </div> <div class="small-12 medium-12 large-9 columns"> <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/> </div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-6 columns"> <span class="prefix">Total number of samples (including replicates)</span> </div> <div class="small-12 medium-12 large-6 columns"> <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/> </div> </div> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2989"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option value="AW">Aruba</option> <option value="AU">Australia</option> <option value="AT">Austria</option> <option value="AZ">Azerbaijan</option> <option value="BS">Bahamas</option> <option value="BH">Bahrain</option> <option value="BD">Bangladesh</option> <option value="BB">Barbados</option> <option value="BY">Belarus</option> <option value="BE">Belgium</option> <option value="BZ">Belize</option> <option value="BJ">Benin</option> <option value="BM">Bermuda</option> <option value="BT">Bhutan</option> <option value="BO">Bolivia</option> <option value="BQ">Bonaire, Sint Eustatius and Saba</option> <option value="BA">Bosnia and Herzegovina</option> <option value="BW">Botswana</option> <option value="BV">Bouvet Island</option> <option value="BR">Brazil</option> <option value="IO">British Indian Ocean Territory</option> <option value="BN">Brunei Darussalam</option> <option value="BG">Bulgaria</option> <option value="BF">Burkina Faso</option> <option value="BI">Burundi</option> <option value="KH">Cambodia</option> <option value="CM">Cameroon</option> <option value="CA">Canada</option> <option value="CV">Cape Verde</option> <option value="KY">Cayman Islands</option> <option value="CF">Central African Republic</option> <option value="TD">Chad</option> <option value="CL">Chile</option> <option value="CN">China</option> <option value="CX">Christmas Island</option> <option value="CC">Cocos (Keeling) Islands</option> <option value="CO">Colombia</option> <option value="KM">Comoros</option> <option value="CG">Congo</option> <option value="CD">Congo, The Democratic Republic of the</option> <option value="CK">Cook Islands</option> <option value="CR">Costa Rica</option> <option value="CI">Côte d'Ivoire</option> <option value="HR">Croatia</option> <option value="CU">Cuba</option> <option value="CW">Curaçao</option> <option value="CY">Cyprus</option> <option value="CZ">Czech Republic</option> <option value="DK">Denmark</option> <option value="DJ">Djibouti</option> <option value="DM">Dominica</option> <option value="DO">Dominican Republic</option> <option value="EC">Ecuador</option> <option value="EG">Egypt</option> <option value="SV">El Salvador</option> <option value="GQ">Equatorial Guinea</option> <option value="ER">Eritrea</option> <option value="EE">Estonia</option> <option value="ET">Ethiopia</option> <option value="FK">Falkland Islands (Malvinas)</option> <option value="FO">Faroe Islands</option> <option value="FJ">Fiji</option> <option value="FI">Finland</option> <option value="FR">France</option> <option value="GF">French Guiana</option> <option value="PF">French Polynesia</option> <option value="TF">French Southern Territories</option> <option value="GA">Gabon</option> <option value="GM">Gambia</option> <option value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option value="GD">Grenada</option> <option value="GP">Guadeloupe</option> <option value="GU">Guam</option> <option value="GT">Guatemala</option> <option value="GG">Guernsey</option> <option value="GN">Guinea</option> <option value="GW">Guinea-Bissau</option> <option value="GY">Guyana</option> <option value="HT">Haiti</option> <option value="HM">Heard Island and McDonald Islands</option> <option value="VA">Holy See (Vatican City State)</option> <option value="HN">Honduras</option> <option value="HK">Hong Kong</option> <option value="HU">Hungary</option> <option value="IS">Iceland</option> <option value="IN">India</option> <option value="ID">Indonesia</option> <option value="IR">Iran, Islamic Republic of</option> <option value="IQ">Iraq</option> <option value="IE">Ireland</option> <option value="IM">Isle of Man</option> <option value="IL">Israel</option> <option value="IT">Italy</option> <option value="JM">Jamaica</option> <option value="JP">Japan</option> <option value="JE">Jersey</option> <option value="JO">Jordan</option> <option value="KZ">Kazakhstan</option> <option value="KE">Kenya</option> <option value="KI">Kiribati</option> <option value="KP">Korea, Democratic People's Republic of</option> <option value="KR">Korea, Republic of</option> <option value="KW">Kuwait</option> <option value="KG">Kyrgyzstan</option> <option value="LA">Lao People's Democratic Republic</option> <option value="LV">Latvia</option> <option value="LB">Lebanon</option> <option value="LS">Lesotho</option> <option value="LR">Liberia</option> <option value="LY">Libya</option> <option value="LI">Liechtenstein</option> <option value="LT">Lithuania</option> <option value="LU">Luxembourg</option> <option value="MO">Macao</option> <option value="MK">Macedonia, Republic of</option> <option value="MG">Madagascar</option> <option value="MW">Malawi</option> <option value="MY">Malaysia</option> <option value="MV">Maldives</option> <option value="ML">Mali</option> <option value="MT">Malta</option> <option value="MH">Marshall Islands</option> <option value="MQ">Martinique</option> <option value="MR">Mauritania</option> <option value="MU">Mauritius</option> <option value="YT">Mayotte</option> <option value="MX">Mexico</option> <option value="FM">Micronesia, Federated States of</option> <option value="MD">Moldova</option> <option value="MC">Monaco</option> <option value="MN">Mongolia</option> <option value="ME">Montenegro</option> <option value="MS">Montserrat</option> <option value="MA">Morocco</option> <option value="MZ">Mozambique</option> <option value="MM">Myanmar</option> <option value="NA">Namibia</option> <option value="NR">Nauru</option> <option value="NP">Nepal</option> <option value="NL">Netherlands</option> <option value="NC">New Caledonia</option> <option value="NZ">New Zealand</option> <option value="NI">Nicaragua</option> <option value="NE">Niger</option> <option value="NG">Nigeria</option> <option value="NU">Niue</option> <option value="NF">Norfolk Island</option> <option value="MP">Northern Mariana Islands</option> <option value="NO">Norway</option> <option value="OM">Oman</option> <option value="PK">Pakistan</option> <option value="PW">Palau</option> <option value="PS">Palestine, State of</option> <option value="PA">Panama</option> <option value="PG">Papua New Guinea</option> <option value="PY">Paraguay</option> <option value="PE">Peru</option> <option value="PH">Philippines</option> <option value="PN">Pitcairn</option> <option value="PL">Poland</option> <option value="PT">Portugal</option> <option value="PR">Puerto Rico</option> <option value="QA">Qatar</option> <option value="RE">Réunion</option> <option value="RO">Romania</option> <option value="RU">Russian Federation</option> <option value="RW">Rwanda</option> <option value="BL">Saint Barthélemy</option> <option value="SH">Saint Helena, Ascension and Tristan da Cunha</option> <option value="KN">Saint Kitts and Nevis</option> <option value="LC">Saint Lucia</option> <option value="MF">Saint Martin (French part)</option> <option value="PM">Saint Pierre and Miquelon</option> <option value="VC">Saint Vincent and the Grenadines</option> <option value="WS">Samoa</option> <option value="SM">San Marino</option> <option value="ST">Sao Tome and Principe</option> <option value="SA">Saudi Arabia</option> <option value="SN">Senegal</option> <option value="RS">Serbia</option> <option value="SC">Seychelles</option> <option value="SL">Sierra Leone</option> <option value="SG">Singapore</option> <option value="SX">Sint Maarten (Dutch part)</option> <option value="SK">Slovakia</option> <option value="SI">Slovenia</option> <option value="SB">Solomon Islands</option> <option value="SO">Somalia</option> <option value="ZA">South Africa</option> <option value="GS">South Georgia and the South Sandwich Islands</option> <option value="ES">Spain</option> <option value="LK">Sri Lanka</option> <option value="SD">Sudan</option> <option value="SR">Suriname</option> <option value="SS">South Sudan</option> <option value="SJ">Svalbard and Jan Mayen</option> <option value="SZ">Swaziland</option> <option value="SE">Sweden</option> <option value="CH">Switzerland</option> <option value="SY">Syrian Arab Republic</option> <option value="TW">Taiwan</option> <option value="TJ">Tajikistan</option> <option value="TZ">Tanzania</option> <option value="TH">Thailand</option> <option value="TL">Timor-Leste</option> <option value="TG">Togo</option> <option value="TK">Tokelau</option> <option value="TO">Tonga</option> <option value="TT">Trinidad and Tobago</option> <option value="TN">Tunisia</option> <option value="TR">Turkey</option> <option value="TM">Turkmenistan</option> <option value="TC">Turks and Caicos Islands</option> <option value="TV">Tuvalu</option> <option value="UG">Uganda</option> <option value="UA">Ukraine</option> <option value="AE">United Arab Emirates</option> <option value="GB">United Kingdom</option> <option value="US" selected="selected">United States</option> <option value="UM">United States Minor Outlying Islands</option> <option value="UY">Uruguay</option> <option value="UZ">Uzbekistan</option> <option value="VU">Vanuatu</option> <option value="VE">Venezuela</option> <option value="VN">Viet Nam</option> <option value="VG">Virgin Islands, British</option> <option value="VI">Virgin Islands, U.S.</option> <option value="WF">Wallis and Futuna</option> <option value="EH">Western Sahara</option> <option value="YE">Yemen</option> <option value="ZM">Zambia</option> <option value="ZW">Zimbabwe</option> </select><script> $('#country_selector_quote-2989').selectize(); </script><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">State</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][state]" id="state-2989" maxlength="3" type="text"/><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/> </div> </div> <div class="row collapse" id="email_v"> <div class="small-3 large-2 columns"> <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Project</span> </div> <div class="small-9 large-10 columns"> <textarea name="data[Quote][comment]" placeholder="Describe your project" cols="30" rows="6" id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha66296bb01c8e4"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-2989" class="alert button expand" form="Quote-2989" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { $('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>'); 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$("#Quote-2989 #state-2989").parent().parent().show(); } else if (this.value == 'DE') { $("#Quote-2989 #state-2989").replaceWith('<select name="data[Quote][state]" id="state-2989" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-2989 #state-2989").parent().parent().show(); } else { $("#Quote-2989 #state-2989").parent().parent().hide(); $("#Quote-2989 #state-2989").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2989" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="wgbs-service" data-reveal-id="quoteModal-2989" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">WGBS (Whole Genome Bisulfite Sequencing) and EM...</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/rrbs-service"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">G02020000</span> </div> <div class="small-6 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Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=2836" id="Quote-2836" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2836" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RGxyN3VUNERGenBPUExVK3k0L0Ivdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="rrbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Service Information</h2> </div> <div class="small-12 large-12 columns"> <h4>Which services are you interested in?</h4> </div> <div class="small-12 large-12 columns"> <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium MethylationEPIC Array v2" id="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2" /><label for="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2">Infinium MethylationEPIC Array v2</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Human Methylome" id="QuoteEpigenomicsServiceHumanMethylome" /><label for="QuoteEpigenomicsServiceHumanMethylome">Human Methylome</label></div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-3 columns"> <span class="prefix">Sample species</span> </div> <div class="small-12 medium-12 large-9 columns"> <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/> </div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-6 columns"> <span class="prefix">Total number of samples (including replicates)</span> </div> <div class="small-12 medium-12 large-6 columns"> <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/> </div> </div> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span 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</div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2836"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option 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bisulfite sequencing (RRBS) </strong> <span>enables </span><span>genome-s</span><span>cale </span>DNA methylation<span> analysis</span> at the single nucleotide level <span>in any vertebrate species. </span><span>The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of</span> whole genome sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent<span> biologically relevant target</span> CpG-rich regions including <span>promoters and </span>CpG islands.<span> Our RRBS service makes this technology widely available and provides high coverage (up to 7 million CpGs</span><span> detected </span><span>in human samples).</span></p> <h2>Genome-scale DNA methylation analysis</h2> <ul class="square"> <li>Robust and cost-effective solution with reliable results</li> <li>Capability to use gDNA inputs down to 25 ng</li> <li>Use of UDIs enhance sequencing results</li> <li>UMI technology eliminates PCR duplicates</li> <li>High coverage - up to 7 million CpGs detected in human samples</li> <li>Single nucleotide resolution</li> <li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li> <li>Suitable for any vertebrate species</li> </ul> <h2>Complete end-to-end service</h2> <ul class="square"> <li><span><span class="ui-provider ee cfi bsv cfj cfk cfl cfm cfn cfo cfp cfq cfr cfs cft cfu cfv cfw cfx cfy cfz cga cgb cgc cgd cge cgf cgg cgh cgi cgj cgk cgl cgm cgn cgo" dir="ltr">From DNA QC to sequencing raw data</span></span></li> <li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2</a> widely adopted</li> <li>As little as 25 ng for any vertebrate species</li> <li>Differentially methylated site analysis <span>using <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> </div> <div class="extra-spaced"> <h2></h2> </div> <p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>', 'label1' => 'Services Workflow', 'info1' => '<div class="row"> <div class="small-6 columns"> <h4>RRBS Service includes:</h4> <table style="width: 905px;"> <tbody> <tr> <td style="width: 264px;"><strong>QC of the genomic DNA</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Measurement of DNA concentration </li> <li>Assessment of DNA quality</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>MspI digestion</li> <li>Library preparation (ends preparation, adaptor ligation)</li> <li>Size selection</li> <li>Sample pooling</li> <li>Bisulfite conversion</li> <li>Library amplification and clean-up</li> <li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Deep sequencing</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li> <li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li> <li>7 million CpGs (on average) for human samples</li> <li>7-11x CpG coverage (on average) for human samples</li> </ul> </td> </tr> </tbody> </table> </div> </div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Bioinformatics Analysis', 'info2' => '<div class="row"> <div class="small-6 columns"> <table style="width: 895px;"> <thead> <tr> <th style="width: 262px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr> <td style="width: 262px;"><strong>Standard</strong></td> <td style="width: 624px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr> <td style="width: 262px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> </tbody> </table> </div> </div> <div id="ConnectiveDocSignExtentionInstalled" 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'services' ), 'language' => 'jp', 'meta_keywords' => 'Targeted DNA methylation service', 'meta_description' => 'Targeted DNA methylation service', 'meta_title' => 'Targeted DNA methylation service', 'product' => array( 'Product' => array( 'id' => '2992', 'antibody_id' => null, 'name' => 'Targeted DNA methylation 受託サービス', 'description' => '<p class="normal" style="text-align: justify; line-height: 150%;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Targeted DNA methylation 受託サービスは、ゲノム全体の既知の正確な位置でのシトシンメチル化の定量的研究を可能にします。関心領域の大きさに応じて、バイサルファイトパイロシーケンシング、バイサルファイトアンプリコンシーケンシング（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">BSAS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）、およびプローブによるカスタムキャプチャーなどの数多くの方法論を用いることが可能です。これらの技術は、単一ヌクレオチド分解能で標的領域のメチル化状態を定量的に検出するために、<em>バイサルファイト変換</em>とその後のシーケンシングに共通の方法論を共有しています。</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; color: #222222;"><o:p></o:p></span></p>', 'label1' => '詳細', 'info1' => '<p style="text-align: left;">Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:</p> <table style="width: 941px;"> <thead> <tr style="height: 69px;"> <th style="width: 218px; height: 69px;"> <h4><strong>Workflow</strong></h4> </th> <th style="width: 402px; height: 69px;"> <h4><strong>Description</strong></h4> </th> <th style="width: 312px; height: 69px;"> <h4><strong>Region of interest</strong></h4> </th> </tr> </thead> <tbody> <tr style="height: 0;"> <td></td> <td></td> <td></td> </tr> <tr style="height: 132px; vertical-align: top;"> <td style="width: 218px; height: 132px;"> <p style="text-align: left;"><strong>PCR Enrichment</strong></p> </td> <td style="width: 402px; height: 132px;"> <ul style="list-style-type: disc;"> <li>(gDNA shearing on Bioruptor Pico)</li> <li>Bisulfite conversion</li> <li>PCR primer design</li> <li>PCR amplification optimization</li> <li>Library preparation</li> <li>QC of final libraries (DNA concentration, analysis of the profile)</li> <li>Sequencing on Illumina platform</li> </ul> </td> <td style="width: 312px; height: 132px; text-align: left;"> <ul style="list-style-type: disc;"> <li style="text-align: left;">Several, long DNA regions</li> <li style="text-align: left;">Total size up to 200kb</li> </ul> </td> </tr> <tr style="height: 157px; 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height: 123px;"><strong>Standard</strong></td> <td style="width: 624px; height: 123px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr style="height: 146px;"> <td style="width: 262px; height: 146px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px; height: 146px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)</li> </ul> </td> </tr> </tbody> </table> <h4></h4>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '', 'catalog_number' => 'G02070000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'targeted-dna-methylation-service', 'meta_title' => 'Targeted DNA methylation service', 'meta_keywords' => 'Targeted DNA methylation service', 'meta_description' => 'Targeted DNA methylation service', 'modified' => '2021-03-08 15:31:23', 'created' => '2018-09-06 10:54:45', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $carrousel_order = array( (int) 0 => 'services' ) $language = 'jp' $meta_keywords = 'Targeted DNA methylation service' $meta_description = 'Targeted DNA methylation service' $meta_title = 'Targeted DNA methylation service' $product = array( 'Product' => array( 'id' => '2992', 'antibody_id' => null, 'name' => 'Targeted DNA methylation 受託サービス', 'description' => '<p class="normal" style="text-align: justify; line-height: 150%;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Targeted DNA methylation 受託サービスは、ゲノム全体の既知の正確な位置でのシトシンメチル化の定量的研究を可能にします。関心領域の大きさに応じて、バイサルファイトパイロシーケンシング、バイサルファイトアンプリコンシーケンシング（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">BSAS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）、およびプローブによるカスタムキャプチャーなどの数多くの方法論を用いることが可能です。これらの技術は、単一ヌクレオチド分解能で標的領域のメチル化状態を定量的に検出するために、<em>バイサルファイト変換</em>とその後のシーケンシングに共通の方法論を共有しています。</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; color: #222222;"><o:p></o:p></span></p>', 'label1' => '詳細', 'info1' => '<p style="text-align: left;">Targeted Bisulfite Sequencing Service will choose between two methodologies depending on the size of the region of interest:</p> <table style="width: 941px;"> <thead> <tr style="height: 69px;"> <th style="width: 218px; height: 69px;"> <h4><strong>Workflow</strong></h4> </th> <th style="width: 402px; height: 69px;"> <h4><strong>Description</strong></h4> </th> <th style="width: 312px; height: 69px;"> <h4><strong>Region of interest</strong></h4> </th> </tr> </thead> <tbody> <tr style="height: 0;"> <td></td> <td></td> <td></td> </tr> <tr style="height: 132px; vertical-align: top;"> <td style="width: 218px; height: 132px;"> <p style="text-align: left;"><strong>PCR Enrichment</strong></p> </td> <td style="width: 402px; height: 132px;"> <ul style="list-style-type: disc;"> <li>(gDNA shearing on Bioruptor Pico)</li> <li>Bisulfite conversion</li> <li>PCR primer design</li> <li>PCR amplification optimization</li> <li>Library preparation</li> <li>QC of final libraries (DNA concentration, analysis of the profile)</li> <li>Sequencing on Illumina platform</li> </ul> </td> <td style="width: 312px; height: 132px; text-align: left;"> <ul style="list-style-type: disc;"> <li style="text-align: left;">Several, long DNA regions</li> <li style="text-align: left;">Total size up to 200kb</li> </ul> </td> </tr> <tr style="height: 157px; 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height: 123px;"><strong>Standard</strong></td> <td style="width: 624px; height: 123px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr style="height: 146px;"> <td style="width: 262px; height: 146px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px; height: 146px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)</li> </ul> </td> </tr> </tbody> </table> <h4></h4>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '', 'catalog_number' => 'G02070000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'targeted-dna-methylation-service', 'meta_title' => 'Targeted DNA methylation service', 'meta_keywords' => 'Targeted DNA methylation service', 'meta_description' => 'Targeted DNA methylation service', 'modified' => '2021-03-08 15:31:23', 'created' => '2018-09-06 10:54:45', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '3022', 'antibody_id' => null, 'name' => 'Bioinformatics Data Mining Service', 'description' => '<h2 class="text-center"><span class="diacol">New!</span> <br />Data mining using machine learning (AI) for unique epigenetic data insights</h2> <center><img src="https://www.diagenode.com/img/product/data-mining-long.png" /></center> <p></p> <div id="paper" style="text-align: center;"> <h4><a href="https://www.youtube.com/watch?v=KXjnSHz3Jk8">Watch the webinar to gain insights on how data mining can be applied for epigenetics applications</a></h4> </div> <center><iframe width="560" height="315" src="https://www.youtube.com/embed/KXjnSHz3Jk8" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen="allowfullscreen"></iframe></center> <p></p> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <blockquote style="padding-bottom: 0;"><span class="label-green" style="margin-bottom: 16px; margin-left: -22px; font-size: 22px;">WHITE PAPERS</span> <div id="portal" class="main-portal"> <div class="portal-inner"><nav class="portal-nav" style="text-align: left;"> <ul data-tab="" class="tips-menu"> <li><a href="#panel1" class="tips portal button">Smokers vs non-smokers </a></li> <li><a href="#panel2" class="tips portal button">Breast cancer</a></li> </ul> </nav></div> </div> <div class="tabs-content"> <div class="content active" id="panel1"> <h3 style="margin-top: 0;">Powerful new insights with epigenetic data mining.<br /> A study to distinguish smokers from non-smokers using just one droplet of blood</h3> <p>Next generation sequencing in combination with sophisticated bioinformatics technologies for genomic, transcriptomic and epigeneomic analyses have enormous potential to establish new biomarkers for disease diagnostics, enabling true precision medicine. Analyses of liquid biopsies to measure thousands of different data points simultaneously in easily accessible body fluids (e.g. blood, urine, and saliva) are extremely promising for such biomarker studies.<br />(...)</p> <a href="https://www.diagenode.com/en/pages/form-bioinfo" class="alert small button" target="_blank">Read more</a></div> <div class="content" id="panel2"> <h3 style="margin-top: 0;">Data mining on DNA methylation data in cancer samples<br />Distinguishing normal from breast cancer tissue</h3> <p>Breast cancer is the most commonly occurring cancer in women and the second most common cancer overall.</p> <p>One important aspect of cancer tissues it that they differ from normal tissues in their epigenetic make up, especially in the DNA methylation pattern. In normal cells methylation assures the proper regulation of gene expression and stable gene silencing. DNA methylation is associated with histone modifications, and the interplay of these epigenetic modifications is crucial to regulate the functioning of the genome by changing chromatin architecture.<br />(...)</p> <a href="https://www.diagenode.com/en/pages/form-bioinfo?app_note=23" class="alert small button" target="_blank">Read more</a></div> </div> </blockquote> </div> </div> <p>Diagenode's new data mining service utilizes methods at the frontier of machine learning, statistics, and database systems. This enhanced service supports new discoveries that were previously not possible by analyzing patterns in large data sets to give informative new insights.</p> <p>If you have data from patient cohorts, single cell analyses or any other heterogeneous scenarios, our service team provides enhanced support with optimal data analysis using our latest data mining capabilities. Specifically, our team applies machine learning technologies to find previously undiscovered or unobvious relationships within and across datasets. This advanced technology allows discovery of informative features from mass data, essentially “finding a needle in a haystack.”</p> <p>Diagenode utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data. Our goal is to build strong classifiers that separate data into two or more classes or groups depending on associated data.</p> <p class="extra-spaced">Different and multiple -omics data classes can be mined simultaneously. Integration with phenotypic and/or clinical data is also possible. We offer data mining services for several data classes including:</p> <table class="extra-spaced"> <tbody> <tr> <td><strong>Epigenetic data</strong></td> <td><strong>Transcriptomic data</strong></td> </tr> <tr> <td> <p>DNA Methylation (RRBS, WGBS, EPIC arrays)</p> <p>ChIP-sequencing</p> <p>ATAC-seq</p> </td> <td> <p>mRNA-sequencing</p> <p>Small and long non coding RNA</p> <p>Single-cell RNA-sequencing</p> </td> </tr> </tbody> </table> <p></p> <p><strong>Biological Interpretation</strong></p> <p class="extra-spaced">Machine learning classifiers also mirror the underlying biological differences between classes and are used to uncover the molecular processes at work. In order to achieve this, we offer biological interpretation services and pathway mining analyses for your data.</p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Data mining modules ', 'info1' => '<ol> <li><strong>Feasibility study</strong></li> </ol> <ul> <li>Assessment of data characteristics and <span></span>applicability of different machine learning (ML)</li> <li>Prototypic analyses:</li> <li>Initial feasibility report</li> </ul> <p></p> <ol start="2"> <li><strong>Data Mining</strong></li> </ol> <ul> <li>Machine learning on data</li> <li>Data evaluation and validation</li> <li>Report generation</li> </ul> <p> </p> <ol start="3"> <li><strong>Data Interpretation</strong></li> </ol> <ul> <li>Integration of background knowledge</li> <li>Functional interpretation / pathway mining</li> <li>Scientific reporting</li> </ul> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'Integration with wet lab services and bioinformatics', 'info2' => '<p>A clear advantage of <strong>Diagenode’s data mining services</strong> is the close connection with<span> </span>other service offerings like <strong>wet lab analysis services</strong> and <strong>bioinformatics services</strong>. You can retrieve a full service package from a single source.</p> <p><a href="https://www.diagenode.com/en/categories/Services">Read about our wetlab services</a></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => 'Custom', 'catalog_number' => 'G02100000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'data-mining-service', 'meta_title' => 'Bioinformatics Data Mining Service | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Diagenode Data Mining Services utilizes multiple algorithms to achieve advanced data mining and uses the most optimal combination of algorithms specific to your data.', 'modified' => '2020-12-21 08:05:15', 'created' => '2019-04-08 16:43:26', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2989', 'antibody_id' => null, 'name' => 'WGBS (Whole Genome Bisulfite Sequencing) and EM-seq (Enzymatic Methylation)受託サービス ', 'description' => '<p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">Whole-genome bisulfite sequencing（</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">WGBS</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">）は、</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">DNA</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">メチル化プロファイリングの中でも最も包括的な次世代シーケンシングであり、ゲノム全体で</span><span lang="EN" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">5-mC</span><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">の単一塩基分解能を可能にします。</span></p> <p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;">同じ場所での変換されていないシトシンと変換されたシトシンの割合を比較することにより、メチル化レベルが決定されます。</span></p> <p class="normal" style="text-align: justify; line-height: 150%; background: white;"><span lang="FR" style="font-size: 12.0pt; line-height: 150%; font-family: 'Arial Unicode MS'; color: #222222;"></span></p> <pre class="tw-data-text tw-text-large tw-ta" data-placeholder="Translation" id="tw-target-text" dir="ltr"><span class="Y2IQFc" lang="ja"></span><span lang="EN" style="font-family: 'Arial Unicode MS'; color: #b21329;">DNA</span><span lang="FR" style="font-family: 'Arial Unicode MS'; color: #b21329;">メチル化研究において最も高いカバレッジ</span></pre> <ul> <li class="normal"><span>ゲノム全体のDNAメチル化と単一塩基分解能スクリーニング</span></li> <li class="normal">低密度と反復領域における5mCの高カバレッジ</li> <li class="normal">専任の科学者がハイタッチコミュニケーションで各プロジェクトをサポート</li> <li class="normal">包括的なサービス - バイサルファイト処理、ライブラリーの調製、シーケンシングと解析</li> </ul> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '詳細', 'info1' => '<h4>WGBS Service includes:</h4> <table style="width: 942px;"> <tbody> <tr style="text-align: left; height: 81px;"> <td style="width: 469px; height: 81px;" colspan="2"> <p><strong>QC of the genomic DNA</strong></p> <p><strong></strong></p> </td> <td style="width: 766px; height: 81px;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">Measurement of DNA concentration</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Assessment of DNA quality</span></li> </ul> </td> </tr> <tr style="height: 129px;"> <td style="width: 375px; height: 236px;" rowspan="2"> <p style="text-align: left;"><strong>Library Preparation</strong></p> <p style="text-align: left;"><strong></strong></p> </td> <td style="width: 94px; height: 129px;"> <p style="text-align: center;"><strong>WGBS</strong></p> </td> <td style="width: 766px; height: 129px;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">gDNA shearing on Bioruptor Pico (not necessary for cfDNA or FFPE)</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Bisulfite conversion</span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">Library preparation </span></li> <li style="font-weight: 400;"><span style="font-weight: 400;">QC of the WGBS libraries (DNA concentration, analysis of the profile)</span></li> </ul> </td> </tr> <tr style="height: 107px;"> <td style="width: 94px; text-align: center; height: 107px; background-color: #f9f9f9;"><strong>EM-seq</strong></td> <td style="width: 766px; height: 107px; background-color: #f9f9f9;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;">gDNA shearing on Bioruptor Pico (not necessary for cfDNA or FFPE)</span></li> <li style="font-weight: 400;">Library preparation with Enzymatic conversion</li> <li style="font-weight: 400;">QC of EM-seq libraries (DNA concentration, analysis of the profile)</li> </ul> <span style="font-weight: 400;"></span></td> </tr> <tr style="height: 129px;"> <td style="width: 469px; height: 129px; background-color: white;" colspan="2"> <p><strong>Deep sequencing</strong></p> <p><strong></strong></p> </td> <td style="width: 766px; height: 129px; background-color: white;"> <ul> <li style="font-weight: 400;"><span style="font-weight: 400;"><span>Samples are sequenced on Illumina platform, paired-end reads of 150bp length (PE150) </span> </span></li> <li style="font-weight: 400;">400M raw reads on average per samples (when pooling 6 samples/lane)</li> <li style="font-weight: 400;">Theoretical Coverage >30X for human, mouse and rat samples</li> <li style="font-weight: 400;"><span style="font-weight: 400;">Detection of >50 million CpGs with 6-9X average CpG coverage for human samples </span></li> </ul> </td> </tr> </tbody> </table> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'バイオインフォマティック解析', 'info2' => '<table style="width: 895px;"> <thead> <tr style="height: 69px;"> <th style="width: 262px; height: 69px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px; height: 69px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr style="height: 123px;"> <td style="width: 262px; height: 123px;"><strong>Standard</strong></td> <td style="width: 624px; height: 123px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr style="height: 146px;"> <td style="width: 262px; height: 146px;"><strong>Differential methylation analysis</strong></td> <td style="width: 624px; height: 146px;"> <ul> <li>Methylation level analysis</li> <li>Differentially methylated CpGs (DMCs) analysis</li> <li>Differentially methylated regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …) and for CpG island locations (islands, shores, shelves, ...)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr style="height: 82px;"> <td style="width: 262px; height: 82px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px; height: 82px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr style="height: 86px;"> <td style="width: 262px; height: 86px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px; height: 86px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> <tr style="height: 86px;"> <td style="width: 262px; height: 86px;"> <p><strong>Data mining</strong></p> </td> <td style="width: 624px; height: 86px;"> <ul> <li><span>Biomarker discovery</span></li> <li><span>Determination of methylation pattern/signature that can be predictive and discriminate between different groups/conditions</span></li> </ul> </td> </tr> </tbody> </table> <h2></h2> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '', 'catalog_number' => 'G02040000', 'old_catalog_number' => '', 'sf_code' => '', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'wgbs-service', 'meta_title' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'meta_keywords' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'meta_description' => 'WGBS service (Whole Genome Bisulfite Sequencing)', 'modified' => '2022-04-11 04:55:23', 'created' => '2018-09-06 10:51:07', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '2836', 'antibody_id' => null, 'name' => 'RRBS Service (Reduced Representation Bisulfite Sequencing)', 'description' => '<p><a href="https://www.diagenode.com/en/quotes/quote?id=2836"><img alt="RRBS Service" src="https://www.diagenode.com/img/banners/b-page-promo-rrbs.png" /></a></p> <div class="extra-spaced"> <p><strong>Reduced representation bisulfite sequencing (RRBS) </strong> <span>enables </span><span>genome-s</span><span>cale </span>DNA methylation<span> analysis</span> at the single nucleotide level <span>in any vertebrate species. </span><span>The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of</span> whole genome sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent<span> biologically relevant target</span> CpG-rich regions including <span>promoters and </span>CpG islands.<span> Our RRBS service makes this technology widely available and provides high coverage (up to 7 million CpGs</span><span> detected </span><span>in human samples).</span></p> <h2>Genome-scale DNA methylation analysis</h2> <ul class="square"> <li>Robust and cost-effective solution with reliable results</li> <li>Capability to use gDNA inputs down to 25 ng</li> <li>Use of UDIs enhance sequencing results</li> <li>UMI technology eliminates PCR duplicates</li> <li>High coverage - up to 7 million CpGs detected in human samples</li> <li>Single nucleotide resolution</li> <li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li> <li>Suitable for any vertebrate species</li> </ul> <h2>Complete end-to-end service</h2> <ul class="square"> <li><span><span class="ui-provider ee cfi bsv cfj cfk cfl cfm cfn cfo cfp cfq cfr cfs cft cfu cfv cfw cfx cfy cfz cga cgb cgc cgd cge cgf cgg cgh cgi cgj cgk cgl cgm cgn cgo" dir="ltr">From DNA QC to sequencing raw data</span></span></li> <li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2</a> widely adopted</li> <li>As little as 25 ng for any vertebrate species</li> <li>Differentially methylated site analysis <span>using <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> </div> <div class="extra-spaced"> <h2></h2> </div> <p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>', 'label1' => 'Services Workflow', 'info1' => '<div class="row"> <div class="small-6 columns"> <h4>RRBS Service includes:</h4> <table style="width: 905px;"> <tbody> <tr> <td style="width: 264px;"><strong>QC of the genomic DNA</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Measurement of DNA concentration </li> <li>Assessment of DNA quality</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>MspI digestion</li> <li>Library preparation (ends preparation, adaptor ligation)</li> <li>Size selection</li> <li>Sample pooling</li> <li>Bisulfite conversion</li> <li>Library amplification and clean-up</li> <li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Deep sequencing</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li> <li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li> <li>7 million CpGs (on average) for human samples</li> <li>7-11x CpG coverage (on average) for human samples</li> </ul> </td> </tr> </tbody> </table> </div> </div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Bioinformatics Analysis', 'info2' => '<div class="row"> <div class="small-6 columns"> <table style="width: 895px;"> <thead> <tr> <th style="width: 262px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr> <td style="width: 262px;"><strong>Standard</strong></td> <td style="width: 624px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr> <td style="width: 262px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> </tbody> </table> </div> </div> <div id="ConnectiveDocSignExtentionInstalled" 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'search_order' => '', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '42800', 'price_CNY' => '/', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => true, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'rrbs-service', 'meta_title' => 'RRBS Service (Reduced Representation Bisulfite Sequencing) | Diagenode', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'RRBS enables genome-scale DNA methylation analysis at the single nucleotide level in any vertebrate species. Robust and cost-effective solution with high coverage (up to 5 million CpGs in human samples).', 'modified' => '2024-01-10 17:48:39', 'created' => '2016-09-21 16:54:44', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array([maximum depth reached]) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '105', 'position' => '1', 'parent_id' => '121', 'name' => 'DNA Methylation Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-4 large-4 columns"> <div class="panel"><center><img src="http://www.diagenode.com/img/categories/services/dna-workflow.png" alt="DNA-methylation -Diagenode" /></center> <p>The pattern of DNA methylation and histone modification(s) plays an essential role in maintaining cellular function. Abnormal DNA methylation – hypermethylation and hypomethylation - can result in adverse outcomes such as cancer or other disease. The quantification of 5-mC through genome-wide DNA methylation analysis can provide information for detection and prognosis of disease. Our DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.</p> </div> <center><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></center></div> <div class="small-12 medium-8 large-8 columns"> <p>Our Epigenomics Profiling Services helps you study DNA methylation (5mC) and methylation variants (5hmC). Our experienced scientists will guide you to the best solution according to your needs, depending on whether you want to analyze DNA methylation at genome-wide or gene specific scales. They will process the arrays or generate meaningful libraries for DNA sequencing and deliver high quality data. Our bioinformatic experts will closely work with you to provide standard and customized analysis with comprehensive publication-ready figures.</p> <h3><a href="https://www.diagenode.com/en/p/infinium-methylation-epic-array-v2-service">Infinium MethylationEPIC Array Service V2</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 930,000 CpGs detected in human samples at single nucleotide resolution</li> <li>Quantitative interrogation of CpG, non-CpG, and CHH sites</li> <li>Differential methylation analysis <span>using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> <!--<h3><a href="https://www.diagenode.com/en/p/infinium-mouse-methylation-array-service">Infinium Mouse Methylation Array Service</a></h3> <ul> <li>Cost-effective solution with rapid turnaround time</li> <li>Over 285,000 markers detected in mouse samples at single nucleotide resolution</li> <li>Suitable for common laboratory mouse strains</li> <li><span>Differential methylation analysis using our<span> </span><a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul>--> <h3><a href="https://www.diagenode.com/en/p/rrbs-service">Reduced Representation Bisulfite Sequencing (RRBS) Service</a></h3> <ul> <li>Robust and cost-effective solution with reliable results</li> <li>Unique technology enables low DNA inputs down to 25ng</li> <li>Accurate analyses with UDIs and UMIs</li> <li>Up to 7 million CpGs detected in human samples at single nucleotide resolution</li> <li>Detection of methylation patterns in CpG-rich regions across the genome including promoters and CpG islands</li> <li><span>Differential methylation analysis <span>using our</span> <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> <li>Suitable for epigenetic biomarker discovery</li> </ul> <h3><a href="https://www.diagenode.com/en/p/wgbs-service">Whole Genome Bisulfite Sequencing (WGBS) or Enzymatic Methylation(EM-seq) Service</a></h3> <ul> <li>Very powerful solution for genome-wide biomarker discovery using bisulfite or enzymatic conversion</li> <li><span>Evaluation of methylation status of nearly every CpG sites of the entire genome </span>at single nucleotide resolution</li> <li>Detection of global methylation patterns including in low CpG-density regions and outside of CpG islands</li> <li>Identification of regions or even loci with differential methylation levels between groups using our <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></li> </ul> <h3><a href="https://www.diagenode.com/en/p/targeted-dna-methylation-service">Targeted Bisulfite Sequencing Service</a></h3> <ul> <li>Focus on D<span>NA methylation patterning of selective regions with single nucleotide resolution</span></li> <li><span>Choose between two methodologies depending on the size of the region of interest: PCR enrichment or hybridization capture</span></li> <li><span>Enhanced accuracy, sensitivity and specificity while reducing overall cost</span></li> <li><span>Allowing deep sequencing at high coverage levels for rare variant identification</span></li> </ul> <!--<h3><a href="../p/medip-seq-service">Methylated DNA IP Sequencing</a></h3> <ul> <li>Immunoprecipitation assay (antibody-based approach) to study 5-mC genome-wide</li> <li>Resolution of 100-500 bp</li> </ul>--> <h3><a href="../p/human-methylome-service">Human Methylome</a></h3> <h3>Watch our webinar:</h3> <p>Cost-Effective Genome Wide DNA Methylation Analysis using Twist Hybrid-Capture Methylome Panel</p> <a class="popup-youtube" href="#webinar"><img src="https://www.diagenode.com/img/webinar/eshg-miniature.png" class="webinar" alt="Webinar series: Genome Wide DNA Methylation using Twist Hybrid-Capture Methylome Panel" /></a> <p></p> <ul> <li>NGS service assay for comprehensive DNA methylation profiling in human samples from solid or liquid biopsy</li> <li>Optimal biomarker discovery tool for cancer, neurodegenerative, cardiovascular and metabolic diseases</li> <li>High coverage for more than 3.89 million methylation sites (~9 million CpGs at single strand level)</li> </ul> </div> </div>', 'no_promo' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'dna-methylation-profiling-services', 'cookies_tag_id' => '3', 'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation', 'meta_description' => 'Diagenode DNA methylation analysis services include numerous bisulfite sequencing options for targeted or whole genome DNA methylation analysis across any species.', 'meta_title' => 'DNA Methylation Profiling Services EPIC array, RRBS, WGBS, MeDIP/hMeDIP-seq and Targeted DNA methylation analysis | Diagenode', 'modified' => '2023-10-04 16:04:25', 'created' => '2015-11-23 14:29:35', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '121', 'position' => '9', 'parent_id' => null, 'name' => 'Epigenomics Profiling Services', 'description' => '<div class="row"> <div class="small-12 medium-10 large-10 columns text-left"> <p>Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis. </p> </div> <div class="small-12 medium-2 large-2 columns text-left"><img src="https://www.diagenode.com/img/logo-scientist-registered-supplier.png" /></div> </div> <center><img alt="DNA-RNA-Chromatin-Diagenode" src="https://www.diagenode.com/img/categories/services/chromatin-dna-rna.png" /></center> <div class="row"> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #099f92;"> <h3><span class="green">Chromatin</span> analysis</h3> <ul> <li>ChIP-seq analysis Histone modification</li> <li>Promoter analysis</li> <li>Enhancer analysis</li> <li>ChIP-seq analysis Transcription factor </li> <li>(sc)ATAC-seq Chromatin accessibility</li> <li>ChIP-seq Cas9 off-target</li> <li>Customized NGS service</li> </ul> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/chip-seq-service">Learn more</a></p> </div> </div> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #474546;"> <h3><span class="darkgrey">DNA methylation</span> analysis</h3> <ul> <li>Reduced representation bisulfite sequencing (RRBS)</li> <li>Whole genome bisulfite sequencing (WGBS)</li> <li>Targeted methylation</li> <li>MeDIP-seq/hMeDIP-seq</li> <li>Infinium MethylationEPIC array </li> </ul> <p></p> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/dna-methylation-profiling-services">Learn more</a></p> </div> </div> <div class="small-12 medium-4 large-4 columns text-left"> <div class="panel" style="border-color: #b11e33;"> <h3><span class="diacol"><g class="gr_ gr_44 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="44" data-gr-id="44">RNA-seq</g></span> analysis</h3> <ul> <li><span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;">Gene expression profiling</span></li> <li>mRNA<span style="display: inline !important; float: none; background-color: #f8f8f8; color: #333333; font-family: Helvetica,Arial,sans-serif; font-size: 16px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px;"> analysis</span></li> <li>Small non-coding RNA analysis</li> <li><b></b>Whole transcriptome analysis<br /><br /><br /><br /><br /></li> </ul> <p><i class="fa fa-arrow-circle-right"></i> <a href="../categories/rna-seq-category">Learn more</a></p> </div> </div> </div> <h2 style="text-align: center;"><a class="details radius small button" href="https://www.diagenode.com/en/documents/services-flyer">DOWNLOAD OUR FLYER</a></h2> <h2>Why Diagenode</h2> <ul> <li><strong>Expertise and trust</strong>: Recognized epigenetics leader, official partner of <a href="http://www.blueprint-epigenome.eu/"><g class="gr_ gr_45 gr-alert gr_spell gr_inline_cards gr_disable_anim_appear ContextualSpelling ins-del multiReplace" id="45" data-gr-id="45">BLUEPRINT</g></a>, <a href="http://ihec-epigenomes.org/">IHEC</a> <g class="gr_ gr_46 gr-alert gr_gramm gr_inline_cards gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="46" data-gr-id="46">and</g> <a href="https://www.faang.org/">FAANG</a></li> <li><strong>Innovative technology</strong>: Utilization of the signature Bioruptor<sup>®</sup> sonication device for optimal chromatin and DNA shearing and the IP-Star<sup>®</sup> Automation device give reproducible and reliable optimization and results</li> <li><strong>Quality</strong>: Multiple QC steps in all workflows and validated antibodies plus reagents deliver superior data</li> <li><strong>Flexibility</strong>: Extensive range of sample species and sample origins</li> <li><strong>Experience</strong> in epigenomics profiling for a wide range of applications and fields of interest</li> </ul> <div class="extra-spaced"> <ul class="small-block-grid-2 medium-block-grid-3 large-block-grid-3"> <li> <div class="block-distri"><span class="label-green" style="margin-bottom: 16px; 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(SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-3022 #state-3022").parent().parent().show(); } else { $("#Quote-3022 #state-3022").parent().parent().hide(); $("#Quote-3022 #state-3022").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3022" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="data-mining-service" data-reveal-id="quoteModal-3022" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">データマイニングサービス</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/wgbs-service"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">G02040000</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2989" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>our epigenomics services</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=2989" id="Quote-2989" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2989" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RGxyN3VUNERGenBPUExVK3k0L0Ivdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="wgbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Service Information</h2> </div> <div class="small-12 large-12 columns"> <h4>Which services are you interested in?</h4> </div> <div class="small-12 large-12 columns"> <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium MethylationEPIC Array v2" id="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2" /><label for="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2">Infinium MethylationEPIC Array v2</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Human Methylome" id="QuoteEpigenomicsServiceHumanMethylome" /><label for="QuoteEpigenomicsServiceHumanMethylome">Human Methylome</label></div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-3 columns"> <span class="prefix">Sample species</span> </div> <div class="small-12 medium-12 large-9 columns"> <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/> </div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-6 columns"> <span class="prefix">Total number of samples (including replicates)</span> </div> <div class="small-12 medium-12 large-6 columns"> <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/> </div> </div> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2989"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option value="AW">Aruba</option> <option value="AU">Australia</option> <option value="AT">Austria</option> <option value="AZ">Azerbaijan</option> <option value="BS">Bahamas</option> <option value="BH">Bahrain</option> <option value="BD">Bangladesh</option> <option value="BB">Barbados</option> <option value="BY">Belarus</option> <option value="BE">Belgium</option> <option value="BZ">Belize</option> <option value="BJ">Benin</option> <option value="BM">Bermuda</option> <option value="BT">Bhutan</option> <option value="BO">Bolivia</option> <option value="BQ">Bonaire, Sint Eustatius and Saba</option> <option value="BA">Bosnia and Herzegovina</option> <option value="BW">Botswana</option> <option value="BV">Bouvet Island</option> <option value="BR">Brazil</option> <option value="IO">British Indian Ocean Territory</option> <option value="BN">Brunei Darussalam</option> <option value="BG">Bulgaria</option> <option value="BF">Burkina Faso</option> <option value="BI">Burundi</option> <option value="KH">Cambodia</option> <option value="CM">Cameroon</option> <option value="CA">Canada</option> <option value="CV">Cape Verde</option> <option value="KY">Cayman Islands</option> <option value="CF">Central African Republic</option> <option value="TD">Chad</option> <option value="CL">Chile</option> <option value="CN">China</option> <option value="CX">Christmas Island</option> <option value="CC">Cocos (Keeling) Islands</option> <option value="CO">Colombia</option> <option value="KM">Comoros</option> <option value="CG">Congo</option> <option value="CD">Congo, The Democratic Republic of the</option> <option value="CK">Cook Islands</option> <option value="CR">Costa Rica</option> <option value="CI">Côte d'Ivoire</option> <option value="HR">Croatia</option> <option value="CU">Cuba</option> <option value="CW">Curaçao</option> <option value="CY">Cyprus</option> <option value="CZ">Czech Republic</option> <option value="DK">Denmark</option> <option value="DJ">Djibouti</option> <option value="DM">Dominica</option> <option value="DO">Dominican Republic</option> <option value="EC">Ecuador</option> <option value="EG">Egypt</option> <option value="SV">El Salvador</option> <option value="GQ">Equatorial Guinea</option> <option value="ER">Eritrea</option> <option value="EE">Estonia</option> <option value="ET">Ethiopia</option> <option value="FK">Falkland Islands (Malvinas)</option> <option value="FO">Faroe Islands</option> <option value="FJ">Fiji</option> <option value="FI">Finland</option> <option value="FR">France</option> <option value="GF">French Guiana</option> <option value="PF">French Polynesia</option> <option value="TF">French Southern Territories</option> <option value="GA">Gabon</option> <option value="GM">Gambia</option> <option value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option value="GD">Grenada</option> <option value="GP">Guadeloupe</option> <option value="GU">Guam</option> <option value="GT">Guatemala</option> <option value="GG">Guernsey</option> <option value="GN">Guinea</option> <option value="GW">Guinea-Bissau</option> <option value="GY">Guyana</option> <option value="HT">Haiti</option> <option value="HM">Heard Island and McDonald Islands</option> <option value="VA">Holy See (Vatican City State)</option> <option value="HN">Honduras</option> <option value="HK">Hong Kong</option> <option value="HU">Hungary</option> <option value="IS">Iceland</option> <option value="IN">India</option> <option value="ID">Indonesia</option> <option value="IR">Iran, Islamic Republic of</option> <option value="IQ">Iraq</option> <option value="IE">Ireland</option> <option value="IM">Isle of Man</option> <option value="IL">Israel</option> <option value="IT">Italy</option> <option value="JM">Jamaica</option> <option value="JP">Japan</option> <option value="JE">Jersey</option> <option value="JO">Jordan</option> <option value="KZ">Kazakhstan</option> <option value="KE">Kenya</option> <option value="KI">Kiribati</option> <option value="KP">Korea, Democratic People's Republic of</option> <option value="KR">Korea, Republic of</option> <option value="KW">Kuwait</option> <option value="KG">Kyrgyzstan</option> <option value="LA">Lao People's Democratic Republic</option> <option value="LV">Latvia</option> <option value="LB">Lebanon</option> <option value="LS">Lesotho</option> <option value="LR">Liberia</option> <option value="LY">Libya</option> <option value="LI">Liechtenstein</option> <option value="LT">Lithuania</option> <option value="LU">Luxembourg</option> <option value="MO">Macao</option> <option value="MK">Macedonia, Republic of</option> <option value="MG">Madagascar</option> <option value="MW">Malawi</option> <option value="MY">Malaysia</option> <option value="MV">Maldives</option> <option value="ML">Mali</option> <option value="MT">Malta</option> <option value="MH">Marshall Islands</option> <option value="MQ">Martinique</option> <option value="MR">Mauritania</option> <option value="MU">Mauritius</option> <option value="YT">Mayotte</option> <option value="MX">Mexico</option> <option value="FM">Micronesia, Federated States of</option> <option value="MD">Moldova</option> <option value="MC">Monaco</option> <option value="MN">Mongolia</option> <option value="ME">Montenegro</option> <option value="MS">Montserrat</option> <option value="MA">Morocco</option> <option value="MZ">Mozambique</option> <option value="MM">Myanmar</option> <option value="NA">Namibia</option> <option value="NR">Nauru</option> <option value="NP">Nepal</option> <option value="NL">Netherlands</option> <option value="NC">New Caledonia</option> <option value="NZ">New Zealand</option> <option value="NI">Nicaragua</option> <option value="NE">Niger</option> <option value="NG">Nigeria</option> <option value="NU">Niue</option> <option value="NF">Norfolk Island</option> <option 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Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=2836" id="Quote-2836" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2836" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RGxyN3VUNERGenBPUExVK3k0L0Ivdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="rrbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Service Information</h2> </div> <div class="small-12 large-12 columns"> <h4>Which services are you interested in?</h4> </div> <div class="small-12 large-12 columns"> <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium MethylationEPIC Array v2" id="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2" /><label for="QuoteEpigenomicsServiceInfiniumMethylationEPICArrayV2">Infinium MethylationEPIC Array v2</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div> <div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Human Methylome" id="QuoteEpigenomicsServiceHumanMethylome" /><label for="QuoteEpigenomicsServiceHumanMethylome">Human Methylome</label></div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-3 columns"> <span class="prefix">Sample species</span> </div> <div class="small-12 medium-12 large-9 columns"> <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/> </div> </div> <div class="row collapse"> <div class="small-12 medium-12 large-6 columns"> <span class="prefix">Total number of samples (including replicates)</span> </div> <div class="small-12 medium-12 large-6 columns"> <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/> </div> </div> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2836"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option 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value="DM">Dominica</option> <option value="DO">Dominican Republic</option> <option value="EC">Ecuador</option> <option value="EG">Egypt</option> <option value="SV">El Salvador</option> <option value="GQ">Equatorial Guinea</option> <option value="ER">Eritrea</option> <option value="EE">Estonia</option> <option value="ET">Ethiopia</option> <option value="FK">Falkland Islands (Malvinas)</option> <option value="FO">Faroe Islands</option> <option value="FJ">Fiji</option> <option value="FI">Finland</option> <option value="FR">France</option> <option value="GF">French Guiana</option> <option value="PF">French Polynesia</option> <option value="TF">French Southern Territories</option> <option value="GA">Gabon</option> <option value="GM">Gambia</option> <option value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option 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#state-2836").parent().parent().show(); } else { $("#Quote-2836 #state-2836").parent().parent().hide(); $("#Quote-2836 #state-2836").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2836" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="rrbs-service" data-reveal-id="quoteModal-2836" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">RRBS受託サービス (Reduced Representation Bisulfite Se...</h6> </div> </div> </li> ' $related = array( 'id' => '2836', 'antibody_id' => null, 'name' => 'RRBS Service (Reduced Representation Bisulfite Sequencing)', 'description' => '<p><a href="https://www.diagenode.com/en/quotes/quote?id=2836"><img alt="RRBS Service" src="https://www.diagenode.com/img/banners/b-page-promo-rrbs.png" /></a></p> <div class="extra-spaced"> <p><strong>Reduced representation bisulfite sequencing (RRBS) </strong> <span>enables </span><span>genome-s</span><span>cale </span>DNA methylation<span> analysis</span> at the single nucleotide level <span>in any vertebrate species. </span><span>The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of</span> whole genome sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent<span> biologically relevant target</span> CpG-rich regions including <span>promoters and </span>CpG islands.<span> Our RRBS service makes this technology widely available and provides high coverage (up to 7 million CpGs</span><span> detected </span><span>in human samples).</span></p> <h2>Genome-scale DNA methylation analysis</h2> <ul class="square"> <li>Robust and cost-effective solution with reliable results</li> <li>Capability to use gDNA inputs down to 25 ng</li> <li>Use of UDIs enhance sequencing results</li> <li>UMI technology eliminates PCR duplicates</li> <li>High coverage - up to 7 million CpGs detected in human samples</li> <li>Single nucleotide resolution</li> <li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li> <li>Suitable for any vertebrate species</li> </ul> <h2>Complete end-to-end service</h2> <ul class="square"> <li><span><span class="ui-provider ee cfi bsv cfj cfk cfl cfm cfn cfo cfp cfq cfr cfs cft cfu cfv cfw cfx cfy cfz cga cgb cgc cgd cge cgf cgg cgh cgi cgj cgk cgl cgm cgn cgo" dir="ltr">From DNA QC to sequencing raw data</span></span></li> <li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2</a> widely adopted</li> <li>As little as 25 ng for any vertebrate species</li> <li>Differentially methylated site analysis <span>using <a href="https://www.diagenode.com/en/categories/bioinformatics-service">bioinformatics tools</a></span></li> </ul> </div> <div class="extra-spaced"> <h2></h2> </div> <p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>', 'label1' => 'Services Workflow', 'info1' => '<div class="row"> <div class="small-6 columns"> <h4>RRBS Service includes:</h4> <table style="width: 905px;"> <tbody> <tr> <td style="width: 264px;"><strong>QC of the genomic DNA</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Measurement of DNA concentration </li> <li>Assessment of DNA quality</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>MspI digestion</li> <li>Library preparation (ends preparation, adaptor ligation)</li> <li>Size selection</li> <li>Sample pooling</li> <li>Bisulfite conversion</li> <li>Library amplification and clean-up</li> <li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li> </ul> </td> </tr> <tr> <td style="width: 264px;"><strong>Deep sequencing</strong></td> <td style="width: 636px;"> <ul style="list-style-type: circle;"> <li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li> <li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li> <li>7 million CpGs (on average) for human samples</li> <li>7-11x CpG coverage (on average) for human samples</li> </ul> </td> </tr> </tbody> </table> </div> </div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => 'Bioinformatics Analysis', 'info2' => '<div class="row"> <div class="small-6 columns"> <table style="width: 895px;"> <thead> <tr> <th style="width: 262px;"> <h4><strong>Analysis</strong></h4> </th> <th style="width: 624px;"> <h4><strong>Features</strong></h4> </th> </tr> </thead> <tbody> <tr> <td style="width: 262px;"><strong>Standard</strong></td> <td style="width: 624px;"> <ul> <li>FASTQ raw data</li> <li>FASTQC quality control insights</li> <li>Alignment of bisulfite sequencing data against reference genome</li> <li>Methylation calling and extraction</li> <li>Summary statistics</li> </ul> </td> </tr> <tr> <td style="width: 262px;"><strong>Differential methylation analysis<br /></strong></td> <td style="width: 624px;"> <ul> <li>Methylation level analysis</li> <li>Differentially Methylated CpGs (DMCs) analysis</li> <li>Differentially Methylated Regions (DMRs) analysis</li> <li>Annotation of DMCs and DMRs for genomic regions (exons, introns, …)</li> <li>Clustering analysis</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Gene ontology terms analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Enrichment analysis on gene associated with DMCs and DMRs</li> <li>Get functional insights</li> </ul> </td> </tr> <tr> <td style="width: 262px;"> <p><strong>Pathway analysis</strong></p> </td> <td style="width: 624px;"> <ul> <li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li> <li>Get mechanistic insights</li> </ul> </td> </tr> </tbody> </table> </div> </div> <div id="ConnectiveDocSignExtentionInstalled" 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