Diagenode

Targeted DNA methylation 受託サービス

  •  詳細
  •  資料
  •  関連記事
  •   お見積り
カタログ番号
フォーマット
G02070000

Targeted DNA methylation 受託サービスは、ゲノム全体の既知の正確な位置でのシトシンメチル化の定量的研究を可能にします。関心領域の大きさに応じて、バイサルファイトパイロシーケンシング、バイサルファイトアンプリコンシーケンシング(BSAS)、およびプローブによるカスタムキャプチャーなどの数多くの方法論を用いることが可能です。これらの技術は、単一ヌクレオチド分解能で標的領域のメチル化状態を定量的に検出するために、バイサルファイト変換とその後のシーケンシングに共通の方法論を共有しています。

  • 詳細

    Targeted DNA methylation service

    Description

    Best adapted applications

    Bisulfite Pyrosequencing

    • Bisulfite-converted DNA regions  PCR-amplified.
    • One PCR strand sequenced on PyroMark
    • Assay design, primers, and PCR conditions validated
    • Species: human, mouse, rat, and monkey

    Small number of single CpGs or short DNA regions with most of the researcher’s CpGs of interest

    Bisulfite Amplicon Sequencing (BSAS)

    • Bisulfite-converted DNA and target region(s) amplified with several PCR amplicons.
    • Amplicons sequenced using Illumina® platform.
    • Assay, primer design, PCR conditions optimized
    • Species: human

    Several, long DNA regions -- total size up to 200kb

    Custom capture probes

    • Target region(s) selectively captured with  custom-designed RNA baits (Agilent SureSelect).
    • Captured regions are bisulfite-converted and sequenced on Illumina® platform.
    • Species: human

    Large number of very long DNA regions -- total size between 0.2-24Mb

  • バイオインフォマティック解析

    Bisulfite Pyrosequencing

    Output files:

    • PCR primers and PCR condition
    • PDF file with raw pyrograms  
    • Excel file with methylation percentage of each target CpG

    Bisulfite Amplicon Sequencing (BSAS)

    Output files:

    • Report with sequencing and CpG methylation statistics
    • FASTQ files with the original raw reads
    • FastQC and trimming reports for the quality of the sequenced reads  
    • Alignment files in BAM format  
    • Alignment and methylation reports  
    • Methylation files of covered Cytosine in bedGraph

    Custom capture probes

    Output files:  

    • Report with sequencing and CpG methylation statistics
    • FASTQ files with the original raw reads
    • FastQC and trimming reports for the quality of the sequenced reads  
    • Alignment files in BAM format
    • Alignment and methylation reports  
    • Methylation files of covered Cytosine in bedGraph

    Differential methylation analysis for any service is available upon request.

  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Targeted DNA methylation 受託サービス (Diagenode Cat# G02070000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

イベント

  • Japanese Biochemical Society
    Yokohama
    Sep 18-Sep 20, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    Atlanta, Georgia
    Sep 19, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    RTP, North Carolina
    Sep 20, 2019
  • EpiGeneSys
    London
    Sep 22-Sep 24, 2019
 すべてのイベントを見る

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics