MeDIP and hMeDIP sequencing services include:
QC of the genomic DNA |
|
Preparation of MeDIP-seq/hMeDIP-seq libraries* |
|
Deep sequencing |
|
*Full protocol details can be viewed in the MagMeDIP-seq Package manual
Methylated DNA immunoprecipitation sequencing (MeDIP-Seq) is used to study 5-mC genome-wide. In this assay, specific antibodies against 5-mC are used to capture methylated DNA regions using an immunoprecipitation assay. The enrichment of methylated DNA in the immunoprecipitated fraction is determined by sequencing to assess the methylation state of individual regions. Using the same approach it is possible to study DNA hydroxymethylation by using an antibody specific for 5-hmC (hMeDIP-seq).