Diagenode

MeDIP-seq service (Methylated DNA IP Sequencing)

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  •  描述
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目录号
格式
G02050000

Methylated DNA immunoprecipitation sequencing (MeDIP-Seq) is used to study 5-mC genome-wide. In this assay, specific antibodies against 5-mC are used to capture methylated DNA regions using an immunoprecipitation assay. The enrichment of methylated DNA in the immunoprecipitated fraction is determined by sequencing to assess the methylation state of individual regions. 

Excellent method for qualitative analysis of DNA methylation profiles

    • Resolution of 100-500 bp
    • 5-mC coverage in low density and highly repetitive regions
    • Antibody-based approach independent of sequence with high specificity and minimal bias
    • Dedicated scientist handles your project with high touch communication
    • Comprehensive service – Immunoprecipitation, library preparation, sequencing, and analysis

 

  • Description

    MeDIP sequencing services include:

    QC of the genomic DNA

    • Measurement of DNA concentration
    • Assessment of  DNA quality

    Preparation of MeDIP-seq libraries*

    • gDNA shearing on Bioruptor® Pico  
    • Library preparation
    • Immunoprecipitation of methylated DNA using Abs specific to 5-mC
    • Library amplification and clean-up  
    • QC of the MeDIP-seq libraries

    Deep sequencing

    • Samples are sequencedon an Illumina platform  
    • Single-read or paired-end  
    • 50bp to 150bp read length  
    • Greater than or equal to 50M reads per sample
    • Coverage to be defined with customer

    *Full protocol details can be viewed in the MagMeDIP-seq Package manual

  • Bioinformatic analysis

    Customized bioinformatic analysis on request

  •  文档
    Epigenomics Profiling Services FLYER
    Chromatin analysis DNA methylation services RNA-seq analysis
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MeDIP-seq service (Methylated DNA IP Sequencing) (Diagenode Cat# G02050000). Click here to copy to clipboard.

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  • FASEB Biological Methylation: Fundamental Mechanisms
    Porto, Portugal
    Jul 28-Aug 1, 2024
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