Magnetic Methylated DNA Immunopreciptation Package for NGS.
DNA methylation is a key epigenetic mechanism with important regulatory functions in biological processes such as genomic imprinting, control of transcription, embryonic development, X-chromosome inactivation, chromosome stability, and carcinogenesis.
DNA methylation occurs primarily as 5-methylcytosine (5-mC), and the Diagenode MagMeDIP kits take advantage of a specific antibody targeting this 5-mC to immunoprecipitate methylated DNA, which can be analyzed by qPCR or Next-Generation Sequencing (NGS). This methylation analysis is fast and highly specific as the IP has been optimized to specifically select and precipitate the methylated DNA by the use of our 5-mC monoclonal antibody 33D3 (Cat. No. C15200081), optimized buffers and protocol.