Diagenode

MeDIP-seq (Methylated DNA IP Sequencing) 受託サービス

default alt
  •  詳細
  •  資料
  •  関連記事
  •   お見積り
カタログ番号
フォーマット
G02050000

5mCゲノム全体の研究に、メチル化DNA免疫沈降シーケンシング(MeDIP-Seq)を行います。このアッセイでは、免疫沈降の際の5-mCに対する特異的抗体を利用して、メチル化DNA領域を捕捉します。免疫沈降画分中のメチル化DNAの濃縮は、個々の領域のメチル化状態を評価する配列決定によって確定します。

DNAメチル化プロファイルの定性分析に優れた方法

    • 100〜500 bpの解像度
    • 低密度および繰り返し頻度の高い領域での5mCカバレッジ
    • 特異性が高く、偏りの少ない配列などに影響を受けない抗体ベースのアプローチ
    • 専門の科学者がハイタッチコミュニケーションで顧客プロジェクトを管理
    • 包括的なサービス - 免疫沈降、ライブラリーの調製、配列決定、分析

 

  • 詳細

    MeDIP sequencing services include:

    QC of the genomic DNA

    • Measurement of DNA concentration
    • Assessment of  DNA quality

    Preparation of MeDIP-seq libraries*

    • gDNA shearing on Bioruptor® Pico  
    • Library preparation
    • Immunoprecipitation of methylated DNA using Abs specific to 5-mC
    • Library amplification and clean-up  
    • QC of the MeDIP-seq libraries

    Deep sequencing

    • Samples are sequencedon an Illumina platform  
    • Single-read or paired-end  
    • 50bp to 150bp read length  
    • Greater than or equal to 50M reads per sample
    • Coverage to be defined with customer

    *Full protocol details can be viewed in the MagMeDIP-seq Package manual

  • バイオインフォマティック解析

    Standard analysis

    Methylation calling:

    Quality check, alignment of sequencing data to reference genome, and identification of methylated or hydroxymethylated regions. Provided files include FastQC reports, alignment files in BAM format, and peak files in BED format.

    Additional analysis on request:  

    Differential methylation analysis:

    Identification of differential methylation between samples based on previously identified MeDIPseq peaks.  

    Annotation with genomic regions:

    Annotation of MeDIP-seq peaks with genomic regions such as introns, exons, enhancers (when available), promoters, intergenic regions.  

    Gene ontology terms analysis:

    Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved.  

    Pathway analysis:

    Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented.  


    Visualization of specific genomic regions:

    Visualization of results (i.e. sequencing data, peaks) at specific genomic regions (e.g. genes, promoters) in publication-ready images (human, mouse, rat).

  •  資料
    Epigenomics Profiling Services FLYER
    Chromatin analysis DNA methylation services RNA-seq analysis
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MeDIP-seq (Methylated DNA IP Sequencing) 受託サービス (Diagenode Cat# G02050000). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  関連商品

イベント

  • Cours international sur le Génome non-codant 11ème édition
    Paris, France
    May 11-May 18, 2022
  • CLEPIC 2022
    Szczecin, Poland
    Jun 8-Jun 10, 2022
  • European Socienty of Human Genetics - ESHG 2022
    Wien, Austria
    Jun 11-Jun 14, 2022
  • Lausanne Genomics Days 2022
    Lausanne, Switzerland
    Jun 16-Jun 17, 2022
 すべてのイベントを見る

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics