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Bioruptor® Pico 非接触式超声波破碎仪




All-in-one solution
Highly reproducible
Processing of 6-16 samples
User friendly software
Five year warranty
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  •  文档
  •  相关
  •   获得报价
目录号
格式
B01080010
1 unit

自 2004 年以来,Diagenode 不断积累细胞、核酸等生物样本剪切专业知识,用于设计 Bioruptor® Pico 并确保提供最佳的样本制备体验,使其广泛应用于各种研究领域(包括环境研究、毒理学、基因组学和表观基因组学、癌症研究、干细胞和发育、神经科学、临床应用、农业等)。

Bioruptor® Pico 是样本剪切方面的最新创新,一项新的突破,是一种能够剪切核酸样本从 150 bp 至 1 kb 的一体式剪切系统。已开发出的剪切配件和耗材,可供用户灵活选择样体积 (20 µL - 2 mL) 和快速行处理样(最多可同时处理 16 个样本)。配置冷却系统 (Bioruptor® Cooler) 可准确。针对所有研究人员设计的善操作界面,提供了简易和进阶两种操作模式,使初学者和有经验的用户都轻易选择正确的操作方式。 

此外,Diagenode 提供了全面验证过的专用超声管(经济实惠、低运成本,< 1€/$/DNA 样本)和剪切试剂盒,以获得最佳样本质量。 

多功能性应用

  • 二代测序DNA 剪切
  • 染色质剪切
  • RNA 剪切
  • 从组织和细胞中提取蛋白质(也用于质谱分析)
  • FFPE 样本核酸提取
  • 蛋白质聚集研究

  • 参数
    Ultrasonic Sonicator
  • 查看 Bioruptor® Pico 的配件和耗材

    Shearing Accessories

    Name Catalog number Throughput
    Tube holder for 0.2 ml tubes B01201144 16 samples
    Tube holder for 0.65 ml tubes B01201143 12 samples
    Tube holder for 1.5 ml tubes B01201140 6 samples
    15 ml sonication accessories B01200016 6 samples

    Shearing Consumables

    Name Catalog Number
    0.2 ml Pico Microtubes C30010020
    0.65 ml Pico Microtubes C30010011
    1.5 ml Pico Microtubes C30010016
    15 ml Pico Tubes C30010017
    15 ml Pico Tubes & sonication beads C01020031

    Find datasheet for Diagenode tubes here

  •  方案
    DNA shearing guide
    DNA shearing for Next-Generation Sequencing with the Bioruptor Pico
    Download
  • 剪切试剂盒

    Diagenode has optimized a range of solutions for successful chromatin preparation. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.

    For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.

    Kit choice guide

    SAMPLE TYPE SAMPLE INPUT KIT SDS
    CONCENTRATION
    NUCLEI
    ISOLATION
    CELLS
    < 100,000 Chromatin EasyShear Kit
    High SDS
    1%
    CELLS
    > 100,000 Chromatin EasyShear Kit
    Ultra Low SDS
    < 0.1%
    TISSUE
    Chromatin EasyShear Kit
    Ultra Low SDS
    < 0.1%
    PLANT TISSUE
    Chromatin EasyShear Kit
    for Plant
    0.5%
    FFPE SAMPLES
    Chromatin EasyShear Kit
    Low SDS
    0.2%
    CELLS
    Chromatin EasyShear Kit
    Low SDS
    0.2%
    TISSUE
    FFPE SAMPLES

    Guide for optimal chromatin preparation using Chromatin EasyShear Kits Read more

  •  文档
    Bioruptor Pico Quickguide MANUAL
    Download
    Bioruptor Pico Manual MANUAL
    Download
    Bioruptor ® Sonicator Brochure BROCHURE
    Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp...
    Download
    Chromatin Shearing Guide PROTOCOL
    Guide for successful chromatin preparation using the Bioruptor Pico
    Download
    DNA shearing guide PROTOCOL
    DNA shearing for Next-Generation Sequencing with the Bioruptor Pico
    Download
    Which tubes for Bioruptor® Pico DOCUMENT
    Download
    Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit APPLICATION NOTE
    By combining the optimized chemistry of the Arima-HiC kit along with new low input protocols, the...
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Bioruptor® Pico 非接触式超声波破碎仪 (Diagenode Cat# B01080010). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    BAF complexes drive proliferation and block myogenic differentiation in fusion-positive rhabdomyosarcoma
    Laubscher et. al.
    Rhabdomyosarcoma (RMS) is a pediatric malignancy of skeletal muscle lineage. The aggressive alveolar subtype is characterized by t(2;13) or t(1;13) translocations encoding for PAX3- or PAX7-FOXO1 chimeric transcription factors, respectively, and are referred to as fusion positive RMS (FP-RMS). The fusion gene alters...

    A Tumor Suppressor Enhancer of PTEN in T-cell development and leukemia
    L. Tottone at al.
    Long-range oncogenic enhancers play an important role in cancer. Yet, whether similar regulation of tumor suppressor genes is relevant remains unclear. Loss of expression of PTEN is associated with the pathogenesis of various cancers, including T-cell leukemia (T-ALL). Here, we identify a highly conserved distal enh...

    Genomic profiling of T-cell activation suggests increased sensitivity ofmemory T cells to CD28 costimulation.
    Glinos, Dafni A and Soskic, Blagoje and Williams, Cayman and Kennedy, Alanand Jostins, Luke and Sansom, David M and Trynka, Gosia
    T-cell activation is a critical driver of immune responses. The CD28 costimulation is an essential regulator of CD4 T-cell responses, however, its relative importance in naive and memory T cells is not fully understood. Using different model systems, we observe that human memory T cells are more sensitive to CD28 co...

    A genetic variant controls interferon-β gene expression in human myeloidcells by preventing C/EBP-β binding on a conserved enhancer.
    Assouvie, Anaïs and Rotival, Maxime and Hamroune, Juliette and Busso,Didier and Romeo, Paul-Henri and Quintana-Murci, Lluis and Rousselet,Germain
    Interferon β (IFN-β) is a cytokine that induces a global antiviral proteome, and regulates the adaptive immune response to infections and tumors. Its effects strongly depend on its level and timing of expression. Therefore, the transcription of its coding gene IFNB1 is strictly controlled. We have previous...

    BCG Vaccination Induces Long-Term Functional Reprogramming of HumanNeutrophils.
    Moorlag, Simone J C F M and Rodriguez-Rosales, Yessica Alina and Gillard,Joshua and Fanucchi, Stephanie and Theunissen, Kate and Novakovic, Borisand de Bont, Cynthia M and Negishi, Yutaka and Fok, Ezio T and Kalafati,Lydia and Verginis, Panayotis and M
    The tuberculosis vaccine bacillus Calmette-Guérin (BCG) protects against some heterologous infections, probably via induction of non-specific innate immune memory in monocytes and natural killer (NK) cells, a process known as trained immunity. Recent studies have revealed that the induction of trained immunit...

    Macrophage Immune Memory Controls Endometriosis in Mice and Humans.
    Jeljeli, Mohamed and Riccio, Luiza G C and Chouzenoux, Sandrine and Moresi,Fabiana and Toullec, Laurie and Doridot, Ludivine and Nicco, Carole andBourdon, Mathilde and Marcellin, Louis and Santulli, Pietro and Abrão,Mauricio S and Chapron, Charles and
    Endometriosis is a frequent, chronic, inflammatory gynecological disease characterized by the presence of ectopic endometrial tissue causing pain and infertility. Macrophages have a central role in lesion establishment and maintenance by driving chronic inflammation and tissue remodeling. Macrophages can be reprogra...

    UTX/KDM6A suppresses AP-1 and a gliogenesis program during neuraldifferentiation of human pluripotent stem cells.
    Xu, Beisi and Mulvey, Brett and Salie, Muneeb and Yang, Xiaoyang andMatsui, Yurika and Nityanandam, Anjana and Fan, Yiping and Peng, Jamy C
    BACKGROUND: UTX/KDM6A is known to interact and influence multiple different chromatin modifiers to promote an open chromatin environment to facilitate gene activation, but its molecular activities in developmental gene regulation remain unclear. RESULTS: We report that in human neural stem cells, UTX binding correla...

    Epigenetic regulation of the lineage specificity of primary human dermallymphatic and blood vascular endothelial cells.
    Tacconi, Carlotta and He, Yuliang and Ducoli, Luca and Detmar, Michael
    Lymphatic and blood vascular endothelial cells (ECs) share several molecular and developmental features. However, these two cell types possess distinct phenotypic signatures, reflecting their different biological functions. Despite significant advances in elucidating how the specification of lymphatic and blood vasc...

    Combined treatment with CBP and BET inhibitors reverses inadvertentactivation of detrimental super enhancer programs in DIPG cells.
    Wiese, M and Hamdan, FH and Kubiak, K and Diederichs, C and Gielen, GHand Nussbaumer, G and Carcaboso, AM and Hulleman, E and Johnsen, SA andKramm, CM
    Diffuse intrinsic pontine gliomas (DIPG) are the most aggressive brain tumors in children with 5-year survival rates of only 2%. About 85% of all DIPG are characterized by a lysine-to-methionine substitution in histone 3, which leads to global H3K27 hypomethylation accompanied by H3K27 hyperacetylation. Hyperacetyla...

    Methylation in pericytes after acute injury promotes chronic kidneydisease.
    Chou, YH and Pan, SY and Shao, YH and Shih, HM and Wei, SY andLai, CF and Chiang, WC and Schrimpf, C and Yang, KC and Lai, LC andChen, YM and Chu, TS and Lin, SL
    The origin and fate of renal myofibroblasts is not clear after acute kidney injury (AKI). Here, we demonstrate that myofibroblasts were activated from quiescent pericytes (qPericytes) and the cell numbers increased after ischemia/reperfusion injury-induced AKI (IRI-AKI). Myofibroblasts underwent apoptosis during ren...

    Exploring the virulence gene interactome with CRISPR/dCas9 in the humanmalaria parasite.
    Bryant, JM and Baumgarten, S and Dingli, F and Loew, D and Sinha, A andClaës, A and Preiser, PR and Dedon, PC and Scherf, A
    Mutually exclusive expression of the var multigene family is key to immune evasion and pathogenesis in Plasmodium falciparum, but few factors have been shown to play a direct role. We adapted a CRISPR-based proteomics approach to identify novel factors associated with var genes in their natural chromatin context. Ca...

    Targeted bisulfite sequencing for biomarker discovery.
    Morselli, M and Farrell, C and Rubbi, L and Fehling, HL and Henkhaus, Rand Pellegrini, M
    Cytosine methylation is one of the best studied epigenetic modifications. In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with dis...

    Battle of the sex chromosomes: competition between X- and Y-chromosomeencoded proteins for partner interaction and chromatin occupancy drivesmulti-copy gene expression and evolution in muroid rodents.
    Moretti, C and Blanco, M and Ialy-Radio, C and Serrentino, ME and Gobé,C and Friedman, R and Battail, C and Leduc, M and Ward, MA and Vaiman, Dand Tores, F and Cocquet, J
    Transmission distorters (TDs) are genetic elements that favor their own transmission to the detriments of others. Slx/Slxl1 (Sycp3-like-X-linked and Slx-like1) and Sly (Sycp3-like-Y-linked) are TDs which have been co-amplified on the X and Y chromosomes of Mus species. They are involved in an intragenomic conflict i...

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活动

  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
  • Dutch Chromatin Meeting
    Radboud UMC, Geert Grooteplein 15, 6525 EZ Nijmegen, The Netherlands, Experience center, route 292 to 307
    Nov 12, 2021
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