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自 2004 年以来,Diagenode 不断积累细胞、核酸等生物样本剪切专业知识,用于设计 Bioruptor® Pico 并确保提供最佳的样本制备体验,使其广泛应用于各种研究领域(包括环境研究、毒理学、基因组学和表观基因组学、癌症研究、干细胞和发育、神经科学、临床应用、农业等)。
Bioruptor® Pico 是样本剪切方面的最新创新,一项新的突破,是一种能够剪切核酸样本从 150 bp 至 1 kb 的一体式剪切系统。已开发出的剪切配件和耗材,可供用户灵活选择样本体积 (20 µL - 2 mL) 和快速平行处理样本(最多可同时处理 16 个样本)。配置冷却系统 (Bioruptor® Cooler) 可准确控制温度。针对所有研究人员设计的友善操作界面,提供了简易和进阶两种操作模式,使初学者和有经验的用户都轻易选择正确的操作方式。
此外,Diagenode 提供了全面验证过的专用超声管(经济实惠、低运行成本,< 1€/$/DNA 样本)和剪切试剂盒,以获得最佳样本质量。
多功能性应用:
Name | Catalog number | Throughput |
Tube holder for 0.2 ml tubes | B01201144 | 16 samples |
Tube holder for 0.65 ml tubes | B01201143 | 12 samples |
Tube holder for 1.5 ml tubes | B01201140 | 6 samples |
15 ml sonication accessories | B01200016 | 6 samples |
Name | Catalog Number |
0.2 ml Pico Microtubes | C30010020 |
0.65 ml Pico Microtubes | C30010011 |
1.5 ml Pico Microtubes | C30010016 |
15 ml Pico Tubes | C30010017 |
15 ml Pico Tubes & sonication beads | C01020031 |
DNA shearing guide DNA shearing for Next-Generation Sequencing with the Bioruptor Pico | Download |
Diagenode has optimized a range of solutions for successful chromatin preparation. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.
For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.
SAMPLE TYPE | SAMPLE INPUT | KIT | SDS CONCENTRATION |
NUCLEI ISOLATION |
||
---|---|---|---|---|---|---|
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CELLS
|
< 100,000 | Chromatin EasyShear Kit High SDS |
1% | ![]() |
|
CELLS
|
> 100,000 | Chromatin EasyShear Kit Ultra Low SDS |
< 0.1% | ![]() |
||
TISSUE
|
Chromatin EasyShear Kit Ultra Low SDS |
< 0.1% | ![]() |
|||
PLANT TISSUE
|
Chromatin EasyShear Kit for Plant |
0.5% | ![]() |
|||
FFPE SAMPLES
|
Chromatin EasyShear Kit Low SDS |
0.2% | ![]() |
|||
![]() |
||||||
CELLS
|
Chromatin EasyShear Kit Low SDS |
0.2% | ![]() |
|||
TISSUE
|
||||||
FFPE SAMPLES
|
||||||
Bioruptor Pico Quickguide MANUAL | Download |
Bioruptor Pico Manual MANUAL | Download |
Bioruptor ® Sonicator Brochure BROCHURE Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp... | Download |
Chromatin Shearing Guide PROTOCOL Guide for successful chromatin preparation using the Bioruptor Pico | Download |
DNA shearing guide PROTOCOL DNA shearing for Next-Generation Sequencing with the Bioruptor Pico | Download |
Which tubes for Bioruptor® Pico DOCUMENT | Download |
![]() How to properly cite this product in your workDiagenode strongly recommends using this: Bioruptor® Pico 非接触式超声波破碎仪 (Diagenode Cat# B01080010). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Combined treatment with CBP and BET inhibitors reverses inadvertentactivation of detrimental super enhancer programs in DIPG cells. |
Methylation in pericytes after acute injury promotes chronic kidneydisease. |
Exploring the virulence gene interactome with CRISPR/dCas9 in the humanmalaria parasite. |
Targeted bisulfite sequencing for biomarker discovery. |
Battle of the sex chromosomes: competition between X- and Y-chromosomeencoded proteins for partner interaction and chromatin occupancy drivesmulti-copy gene expression and evolution in muroid rodents. |
You are about to request a quote for Tube holder for 1.5 ml tubes - Bioruptor® Pico. Fill out the form below and we will be in touch with you very soon.
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You are about to request a quote for Tube holder for 0.65 ml tubes - Bioruptor® Pico. Fill out the form below and we will be in touch with you very soon.
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You are about to request a quote for Tube holder for 0.2 ml tubes - Bioruptor® Pico. Fill out the form below and we will be in touch with you very soon.
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