Diagenode

H2BK20ac monoclonal antibody

Antibody ChIP-seq grade icon
Catalog Number
Format
Price
C15210010
100 µg
$305.00
  Bulk order

Monoclonal antibody raised in rabbit against histone H2B acetylated at Lys20 (H2BK20ac), using a KLH-conjugated synthetic peptide.

Lot001D
Concentration1 µg/µl
Species reactivityHuman. Other species not tested
TypeMonoclonal
PurityProtein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
HostRabbit
Storage ConditionsStore at -20°C
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 0.5-1 μg/ChIP Fig 1, 2
Western Blotting 1:2,000 Fig 3
Immunofluorescence 1:500 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H2BK20ac
    ChIP assays were performed using human HeLa cells, the Diagenode antibody against H2BK20ac (cat. No. C15210010) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 0.5, 1 and 2.5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the active EIF4A2 and ACTB genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A.
    ChIP-seq
    B.
    ChIP-seq
    C.
    ChIP-seq
    D.
    ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H2BK20ac
    ChIP was performed with 0.5 µg of the Diagenode antibody against H2BK20ac (cat. No. C15210010) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1.5 Mb region of the human X chromosome (figure 2A and B) and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (figure 2C and D).

    WB

    Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H2BK20ac
    Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode monoclonal antibody against H2BK20ac (cat. No. C15210010) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IF

    Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against H2BK20ac
    HeLa cells treated with sodium butyrate were stained with the Diagenode antibody against H2BK20ac No. C15210010, red) diluted 1:200. Actin filaments were stained with fluorescein phalladoin (green).

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    H2BK20ac monoclonal antibody DATASHEET
    Monoclonal antibody raised in rabbit against histone H2B acetylated at Lys20 (H2BK20ac), using a ...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H2BK20ac monoclonal antibody (Diagenode Cat# C15210010 Lot# 001D). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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  • The 12th Annual Meeting of the Japanese Society for Epigenetics
    Jun 24-Jun 25, 2018
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