FOXM1 Antibody - ChIP-seq Grade (C15410232)

Lot	42613
Concentration	1.52 μg/μl
Species reactivity	Human
Type	Polyclonal
Purity	Affinity purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution ^*	References
ChIP/ChIP-seq ^*	2 μg/ChIP	Fig 1, 2
Western Blotting	1:1,000 - 1:10,000	Fig 3
Immunohistochemistry	1:100 - 1:1,000	Fig 4

FOXM1 Antibody ChIP Grade

Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1
ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

FOXM1 Antibody ChIP-seq Grade

FOXM1 Antibody for ChIP-seq

FOXM1 Antibody for ChIP-seq assay

FOXM1 Antibody validated in ChIP-seq

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1
ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).

FOXM1 Antibody validated in Western Blot

Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1
Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

FOXM1 Antibody validated in Immunohistochemistry

Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1
Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.

Target Description

FOXM1 (UniProt/Swiss-Prot entry Q08050) is a transcription factor which is involved in cell proliferation. It regulates the expression of cell cycle genes essential for DNA replication and mitosis such as cyclin B1 and cyclin A2. It may also play a role in DNA breaks repair participating in the DNA damage checkpoint response.

Applications

IHC
Immunohistochemistry Read more

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

ChIP-seq (ab)
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ChIP-qPCR (ab)
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Documents

Datasheet FOXM1 C15410232 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download