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FOXM1 Antibody - ChIP-seq Grade

Catalog Number
Format
Price
C15410232-100
100 μl
$355.00
  Bulk order



Polyclonal antibody raised in rabbit against FOXM1 (forkhead box M1), using a KLH-conjugated synthetic peptide.

Lot42613
Concentration1.52 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP/ChIP-seq * 2 μg/ChIP Fig 1, 2
Western Blotting 1:1,000 - 1:10,000 Fig 3
Immunohistochemistry 1:100 - 1:1,000 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    FOXM1 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXM1
    ChIP assays were performed using HeLa cells, the Diagenode antibody against FOXM1 (Cat. No. C15410232) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the CCNA2 and CCNB1 genes, used as positive controls, and for the IFT80 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    FOXM1 Antibody ChIP-seq Grade

    FOXM1 Antibody for ChIP-seq

    FOXM1 Antibody for ChIP-seq assay

    FOXM1 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXM1
    ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against FOXM1 (Cat. No. C15410232) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 kb region of human chromosome 6 (fig 2A and B), and in two genomic regions surrounding the CCNA2 and CCNB1 positive control genes (fig 2C and D).

    FOXM1 Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against FOXM1
    Whole cell extracts from 293T (30 μg, lane 1) or 293T cells transfected with a FOXM1 expression vector (lane 2) were analysed by Western blot using the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    FOXM1 Antibody validated in Immunohistochemistry

    Figure 4. Immunohistochemistry using the Diagenode antibody directed against FOXM1
    Formalin fixed paraffin embedded human ovarian cancer tissue was stained with the Diagenode antibody against FOXM1 (Cat. No. C15410232) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.

  • Target Description

    FOXM1 (UniProt/Swiss-Prot entry Q08050) is a transcription factor which is involved in cell proliferation. It regulates the expression of cell cycle genes essential for DNA replication and mitosis such as cyclin B1 and cyclin A2. It may also play a role in DNA breaks repair participating in the DNA damage checkpoint response.

  •  Applications
    IHC
    Immunohistochemistry Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet FOXM1 C15410232 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: FOXM1 Antibody - ChIP-seq Grade (Diagenode Cat# C15410232-100 Lot# 42613). Click here to copy to clipboard.

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