Diagenode

>>>   Click for Diagenode’s approach to COVID-19

PPARg Antibody - ChIP-seq Grade

Catalog Number
Format
Price
C15410367
50 µg
$325.00
  Bulk order
Other format



Other names: PPAR-gamma, NR1C3

Polyclonal antibody raised in rabbit against human PPARg ((peroxisome proliferator-activated receptor gamma), using two KLH-conjugated synthetic peptides from the central and the N-terminal part of the protein, respectively.

LotA2896-0014P
Concentration1.9 µg/µl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 5 μg/ChIP Fig 1, 2
ELISA 1:10,000 Fig 3
Western blotting not recommended

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.

  • Validation data

    ChIP results obtained with the Diagenode antibody directed against PPARg
    ChIP was performed with the Diagenode antibody against PPARg (Cat. No. C15410367) on sheared chromatin from 4,000,000 Capan2 cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the HIST1H4C and LAMP1 genes, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A.

    B.

    C.

    D.

    ChIP-seq results obtained with the Diagenode antibody directed against PPARg
    ChIP was performed on sheared chromatin from 4,000,000 Capan2 cells using 5 µg of the Diagenode antibody against PPARg (Cat. No. C15410367) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1.5 Mb region of chromosome 1 (figure 2A and B), in a genomic region of chromosome 6 containing several HIST1 genes (figure 2C), and in a genomic region of the X-chromosome surrounding the LAMP1 positive control gene (figure 2D).

    Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PPARg (Cat. No. C15410367). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.

  •  Applications
  •  Documents
    PPARg polyclonal antibody datasheet DATASHEET
    Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-c...
    Download
  •  Safety sheets
    PPARg polyclonal antibody SDS US en Download
    PPARg polyclonal antibody SDS GB en Download
    PPARg polyclonal antibody SDS ES es Download
    PPARg polyclonal antibody SDS DE de Download
    PPARg polyclonal antibody SDS JP ja Download
    PPARg polyclonal antibody SDS BE nl Download
    PPARg polyclonal antibody SDS BE fr Download
    PPARg polyclonal antibody SDS FR fr Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PPARg Antibody - ChIP-seq Grade (Diagenode Cat# C15410367 Lot# A2896-0014P). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics