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ETO Antibody

ChIP-seq Grade

Catalog Number
Format
Price
C15310001
100 µl
$340.00
  Bulk order
Other format
C15310001-20
20 µl



Alternative names: RUNX1T1, AML1T1, CBFA2T1, CDR, MTG8, ZMYND2

Polyclonal antibody raised in rabbit against human ETO (runt-related transcription factor 1; translocated to, 1 (cyclin D-related)) using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.

LotA710-001
Concentrationnot determined
Species reactivityHuman
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 4 μl/ChIP Fig 1, 2
ELISA 1:100 Fig 3

* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 μl per IP.

  • Validation Data

    ETO Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO
    ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.

    ETO Antibody ChIP-seq Grade

    ETO Antibody for ChIP-seq

    ETO Antibody for ChIP-seq assay

    ETO Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO
    ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

    ETO Antibody ELISA Validation

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300.

  • Target Description

    ETO (UniProtKB/Swiss-Prot entry Q06455) is a transcriptional regulator which belongs to the myeloid translocation gene family. ETO exerts its function by interaction with transcription factors bound to promoters and binding to histone deacetylases. It recruits a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukaemia. This translocation produces a chimeric gene made up of the 5’-region of AML1and the 3’-region of the ETO gene. The chimeric protein is thought to associate with the nuclear corepressor/ histone deacetylase complex to block hematopoietic differentiation.

  •  Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet ETO C15310001 DATASHEET
    Datasheet description    		 Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...    		 Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...    		 Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ETO Antibody (Diagenode Cat# C15310001 Lot# A710-001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    ERG and FLI1 binding sites demarcate targets for aberrant epigenetic regulation by AML1-ETO in acute myeloid leukemia.
    Martens JH, Mandoli A, Simmer F, Wierenga BJ, Saeed S, Singh AA, Altucci L, Vellenga E, Stunnenberg HG
    ERG and FLI1 are closely related members of the ETS family of transcription factors and have been identified as essential factors for the function and maintenance of normal hematopoietic stem cells. Here, genome-wide analysis revealed that both ERG and FLI1 occupy similar genomic regions as AML1-ETO in t(8;21) AMLs ...

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