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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9ac</strong><br />ChIP was performed with the Diagenode antibody against H3K9ac (cat. No. C15210015) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2A-ChIP-seq.jpg" alt="H3K9ac Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2B-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2C-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2D-ChIP-seq.jpg" alt="H3K9ac Antibody validated in ChIP-seq" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K9ac</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 0.5 µg of the Diagenode antibody against H3K9ac (cat. No. C15210015) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the X chromosome and a zoomin to a 600 kb region (fig 2A and B) and in two genomic regions containing the GAPDH and EIF4A2 genes (fig 2C and D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-db.jpg" alt="H3K9ac Antibody Dot Blot validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />To test the cross reactivity of the Diagenode antibody against H3K9ac (cat. No. C15210015), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9ac (cat. No. C15210015). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<div class="small-8 columns">
<p><strong>Figure 5. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9ac (cat. No. C15210015, red) diluted 1:200. Actin was stained with fluorescein phalladoin (green).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-chip.jpg" alt="H3K9ac Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9ac</strong><br />ChIP was performed with the Diagenode antibody against H3K9ac (cat. No. C15210015) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2A-ChIP-seq.jpg" alt="H3K9ac Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2B-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2C-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2D-ChIP-seq.jpg" alt="H3K9ac Antibody validated in ChIP-seq" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K9ac</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 0.5 µg of the Diagenode antibody against H3K9ac (cat. No. C15210015) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the X chromosome and a zoomin to a 600 kb region (fig 2A and B) and in two genomic regions containing the GAPDH and EIF4A2 genes (fig 2C and D).</p>
<p></p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-db.jpg" alt="H3K9ac Antibody Dot Blot validation" /></center></div>
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<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />To test the cross reactivity of the Diagenode antibody against H3K9ac (cat. No. C15210015), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9ac (cat. No. C15210015). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 5. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9ac (cat. No. C15210015, red) diluted 1:200. Actin was stained with fluorescein phalladoin (green).</p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
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<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
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<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-chip.jpg" alt="H3K9ac Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9ac</strong><br />ChIP was performed with the Diagenode antibody against H3K9ac (cat. No. C15210015) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2A-ChIP-seq.jpg" alt="H3K9ac Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2B-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2C-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2D-ChIP-seq.jpg" alt="H3K9ac Antibody validated in ChIP-seq" /></center></div>
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<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K9ac</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 0.5 µg of the Diagenode antibody against H3K9ac (cat. No. C15210015) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the X chromosome and a zoomin to a 600 kb region (fig 2A and B) and in two genomic regions containing the GAPDH and EIF4A2 genes (fig 2C and D).</p>
<p></p>
</div>
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<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-db.jpg" alt="H3K9ac Antibody Dot Blot validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />To test the cross reactivity of the Diagenode antibody against H3K9ac (cat. No. C15210015), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<div class="extra-spaced"></div>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-wb.jpg" alt="H3K9ac Antibody validated in Western blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9ac (cat. No. C15210015). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-if.jpg" alt="H3K9ac Antibody validated in Immunofluorescence" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 5. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9ac (cat. No. C15210015, red) diluted 1:200. Actin was stained with fluorescein phalladoin (green).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-chip.jpg" alt="H3K9ac Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9ac</strong><br />ChIP was performed with the Diagenode antibody against H3K9ac (cat. No. C15210015) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2A-ChIP-seq.jpg" alt="H3K9ac Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2B-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2C-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2D-ChIP-seq.jpg" alt="H3K9ac Antibody validated in ChIP-seq" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K9ac</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 0.5 µg of the Diagenode antibody against H3K9ac (cat. No. C15210015) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the X chromosome and a zoomin to a 600 kb region (fig 2A and B) and in two genomic regions containing the GAPDH and EIF4A2 genes (fig 2C and D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-db.jpg" alt="H3K9ac Antibody Dot Blot validation" /></center></div>
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<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9ac (cat. No. C15210015). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 5. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9ac (cat. No. C15210015, red) diluted 1:200. Actin was stained with fluorescein phalladoin (green).</p>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 µg per IP.</small></p>',
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
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<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
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<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
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<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-chip.jpg" alt="H3K9ac Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9ac</strong><br />ChIP was performed with the Diagenode antibody against H3K9ac (cat. No. C15210015) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2A-ChIP-seq.jpg" alt="H3K9ac Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2B-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2C-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2D-ChIP-seq.jpg" alt="H3K9ac Antibody validated in ChIP-seq" /></center></div>
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<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K9ac</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 0.5 µg of the Diagenode antibody against H3K9ac (cat. No. C15210015) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the X chromosome and a zoomin to a 600 kb region (fig 2A and B) and in two genomic regions containing the GAPDH and EIF4A2 genes (fig 2C and D).</p>
<p></p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-db.jpg" alt="H3K9ac Antibody Dot Blot validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />To test the cross reactivity of the Diagenode antibody against H3K9ac (cat. No. C15210015), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-wb.jpg" alt="H3K9ac Antibody validated in Western blot" /></center></div>
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<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9ac (cat. No. C15210015). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 5. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9ac (cat. No. C15210015, red) diluted 1:200. Actin was stained with fluorescein phalladoin (green).</p>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 µg per IP.</small></p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-chip.jpg" alt="H3K9ac Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9ac</strong><br />ChIP was performed with the Diagenode antibody against H3K9ac (cat. No. C15210015) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2A-ChIP-seq.jpg" alt="H3K9ac Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2B-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2C-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2D-ChIP-seq.jpg" alt="H3K9ac Antibody validated in ChIP-seq" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K9ac</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 0.5 µg of the Diagenode antibody against H3K9ac (cat. No. C15210015) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the X chromosome and a zoomin to a 600 kb region (fig 2A and B) and in two genomic regions containing the GAPDH and EIF4A2 genes (fig 2C and D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-db.jpg" alt="H3K9ac Antibody Dot Blot validation" /></center></div>
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<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />To test the cross reactivity of the Diagenode antibody against H3K9ac (cat. No. C15210015), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9ac (cat. No. C15210015). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 5. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9ac (cat. No. C15210015, red) diluted 1:200. Actin was stained with fluorescein phalladoin (green).</p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
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<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
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<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
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<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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'name' => 'H3K9ac Antibody',
'description' => '<p>Monoclonal antibody raised in rabbit against the region of histone <strong>H3 containing the acetylated lysine 9 (H3K9ac)</strong>, using a KLH-conjugated synthetic peptide.</p>',
'label1' => 'Validation data',
'info1' => '<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-chip.jpg" alt="H3K9ac Antibody ChIP Grade" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9ac</strong><br />ChIP was performed with the Diagenode antibody against H3K9ac (cat. No. C15210015) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2A-ChIP-seq.jpg" alt="H3K9ac Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2B-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2C-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2D-ChIP-seq.jpg" alt="H3K9ac Antibody validated in ChIP-seq" /></center></div>
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<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K9ac</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 0.5 µg of the Diagenode antibody against H3K9ac (cat. No. C15210015) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the X chromosome and a zoomin to a 600 kb region (fig 2A and B) and in two genomic regions containing the GAPDH and EIF4A2 genes (fig 2C and D).</p>
<p></p>
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<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-db.jpg" alt="H3K9ac Antibody Dot Blot validation" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />To test the cross reactivity of the Diagenode antibody against H3K9ac (cat. No. C15210015), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<div class="extra-spaced"></div>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-wb.jpg" alt="H3K9ac Antibody validated in Western blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9ac (cat. No. C15210015). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-if.jpg" alt="H3K9ac Antibody validated in Immunofluorescence" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 5. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9ac (cat. No. C15210015, red) diluted 1:200. Actin was stained with fluorescein phalladoin (green).</p>
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<td>ChIP/ ChIP-seq <sup>*</sup></td>
<td>0.5 - 1 µg per IP</td>
<td>Fig 1, 2</td>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 µg per IP.</small></p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-chip.jpg" alt="H3K9ac Antibody ChIP Grade" /></center></div>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9ac</strong><br />ChIP was performed with the Diagenode antibody against H3K9ac (cat. No. C15210015) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="small-12 columns">A.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2A-ChIP-seq.jpg" alt="H3K9ac Antibody ChIP-seq Grade" /></center><br /> B.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2B-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq" /></center><br /> C.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2C-ChIP-seq.jpg" alt="H3K9ac Antibody for ChIP-seq assay" /></center><br /> D.<center><img src="https://www.diagenode.com/img/product/antibodies/C15210015-fig2D-ChIP-seq.jpg" alt="H3K9ac Antibody validated in ChIP-seq" /></center></div>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against H3K9ac</strong><br />ChIP was performed on sheared chromatin from 500,000 HeLaS3 cells using 0.5 µg of the Diagenode antibody against H3K9ac (cat. No. C15210015) as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the X chromosome and a zoomin to a 600 kb region (fig 2A and B) and in two genomic regions containing the GAPDH and EIF4A2 genes (fig 2C and D).</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-db.jpg" alt="H3K9ac Antibody Dot Blot validation" /></center></div>
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<p><strong>Figure 3. Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />To test the cross reactivity of the Diagenode antibody against H3K9ac (cat. No. C15210015), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K9. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-wb.jpg" alt="H3K9ac Antibody validated in Western blot" /></center></div>
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<p><strong>Figure 4. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9ac (cat. No. C15210015). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15210016-if.jpg" alt="H3K9ac Antibody validated in Immunofluorescence" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 5. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9ac</strong><br />HeLa cells were stained with the Diagenode antibody against H3K9ac (cat. No. C15210015, red) diluted 1:200. Actin was stained with fluorescein phalladoin (green).</p>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 µg per IP.</small></p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode’s collection includes antibodies recognizing:</p>
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<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
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<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> Expert technical support</span></li>
<li><span style="font-weight: 400;"> Sample sizes available</span></li>
<li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li>
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