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AF9 Antibody - ChIP-seq Grade

Catalog Number
100 μl
  Bulk order
Other format

Other names: MLLT3, YEATS3

Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.

ConcentrationNot determined
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal ChIP grade, ChIP-seq grade
PurityWhole antiserum from rabbit containing 0.05% azide.
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution/amount References
ChIP/ChIP-seq * 2 µl/ChIP Fig 1, 2
ELISA 1:100 - 1:500 Fig 3

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.

  • Validation data

    AF9 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9
    ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).

    AF9 Antibody ChIP-seq Grade

    AF9 Antibody for ChIP-seq

    AF9 Antibody for ChIP-seq assay

    AF9 Antibody validated in ChIP-seq

    AF9 Antibody ChIP-seq Grade

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9
    ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).

    AF9 Antibody ELISA Validation

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.

  • Target Description

    AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.

  •  Applications
  •  Documents
    AF9 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a...
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: AF9 Antibody - ChIP-seq Grade (Diagenode Cat# C15310266 Lot# A1474-001). Click here to copy to clipboard.

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