Diagenode

AF9 polyclonal antibody

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310266
100 μl
$305.00
  Bulk order
Other format

Other names: MLLT3, YEATS3

Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a KLHconjugated synthetic peptide containing a sequence from the central region of the protein.

LotA1474-001
ConcentrationNot determined
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal ChIP grade, ChIP-seq grade
PurityWhole antiserum from rabbit containing 0.05% azide.
HostRabbit
StorageStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution/amount References
ChIP * 2 µl/ChIP Fig 1, 2
ELISA 1:100 - 1:500 Fig 3

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9
    ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).

    ChIP-seq A

    ChIP-seq B

    ChIP-seq C

    ChIP-seq D

    ChIP-seq E

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9
    ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).

    ELISA

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.

  • Applications
  • Documents
    AF9 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a...
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  • Publications

    How to properly cite this product in your work

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