Diagenode

HDAC3 polyclonal antibody

Catalog Number
Format
Price
C15410361
50 µg
$315.00
  Bulk order

Other names: HD3, RPD3, RPD3-2, SMAP45

Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.

LotA2633-0040
Concentration1.7 µg/µl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 2 µg/ChIP Fig 1, 2
ELISA 1:10,000 Fig 2
Western Blotting Not recommended

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3
    ChIP was performed with the Diagenode antibody against HDAC3 (cat. No. C15410361) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A. ChIP-seq

    B. ChIP-seq

    C. ChIP-seq

    D.ChIP-seq

    E.ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3
    ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (cat. No. C15410361) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).

    ELISA

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (cat. No. C15410361). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.

  •  Applications
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    HDAC3 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-c...
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  •  Publications

    How to properly cite this product in your work

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