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HDAC3 Antibody - ChIP-seq Grade

Catalog Number
Format
Price
C15410364
(C15410361)
50 µg
$325.00
  Bulk order



Other names: HD3, RPD3, RPD3-2, SMAP45

Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-conjugated synthetic peptides from the central and the C-terminal part of the protein, respectively.

LotA2633-0040
Concentration1.7 µg/µl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 2 µg/ChIP Fig 1, 2
ELISA 1:10,000 Fig 3
Western Blotting Not recommended

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.

  • Validation data

    HDAC3 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC3
    ChIP was performed with the Diagenode antibody against HDAC3 (Cat. No. C15410364) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the CDK4, FAM134A and HNRNPH2 promoters, used as positive controls, and for the MYOD1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A. HDAC3 Antibody ChIP-seq Grade

    B. HDAC3 Antibody for ChIP-seq

    C. HDAC3 Antibody for ChIP-seq assay

    D.HDAC3 Antibody for ChIP-seq assay

    E.HDAC3 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC3
    ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 µg of the Diagenode antibody against HDAC3 (Cat. No. C15410364) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 3 Mb region of chromosome 3 (figure 2A and B) and in three genomic regions surrounding the CDK4, FAM134A and HNRNPH2 positive control genes, respectively (figure 2C, D and E).

    HDAC3 Antibody ELISA validation

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC3 (Cat. No. C15410364). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:450,000.

  • Target Description

    HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and on some other non-histone substrates. Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression. When tethered to a promoter, HDAC3 represses transcription and therefore plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC3 probably participates in the regulation of transcription through its binding to the YY1 transcription factor, increasing YY1 repression. HDAC3 also downregulates p53 and is considered a potential tumor suppressor gene.

  •  Applications
  •  Documents
    HDAC3 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human HDAC3 (Histone deacetylase 3), using two KLH-c...
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  •  Publications

    How to properly cite this product in your work

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