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CHD1 Antibody - ChIP-seq Grade

Catalog Number
Format
Price
C15410334
100 μl
$355.00
  Bulk order



Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain Helicase DNA Binding Protein 1), using a synthetic peptide containing a sequence from the C-terminal part of the protein.

1Manufactured by Bethyl Laboratories, Inc., Texas, USA

LotA301-218A1
Concentration0.2 μg/μl
Species reactivityHuman
TypePolyclonal ChIP grade, ChIP-seq grade
PurityAffinity purified
HostRabbit
Storage ConditionsStore at 4°C
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq* 1 μg/ChIP Fig 1, 2
Western Blotting 1:2,000 Fig 3
IP 3 μg per IP Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation data

    CHD1 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1
    ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A.CHD1 Antibody ChIP-seq Grade
    B.CHD1 Antibody for ChIP-seq
    C.CHD1 Antibody for ChIP-seq assay
    D.CHD1 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1
    ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).

    CHD1 Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1
    Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    CHD1 Antibody validated in IP

    Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200.

  • Target Description

    CHD1 (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.

  •  Applications
    ChIP-qPCR (ab)
    Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
    ChIP-seq (ab)
    Read more
  •  Documents
    CHD1 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain Helicase DNA Binding Protei...
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CHD1 Antibody - ChIP-seq Grade (Diagenode Cat# C15410334 Lot# A301-218A1). Click here to copy to clipboard.

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