Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8
ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).