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CBX8 Antibody - ChIP-seq Grade

Catalog Number
100 μl
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Polyclonal antibody raised in rabbit against human CBX8 (chromobox 8), using a synthetic peptide containing a sequence from the C-terminal part of the protein.

Concentration1 μg/μl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal ChIP grade, ChIP-seq grade
PurityAffinity purified polyclonal antibody.
Storage ConditionsStore at 4°C
Storage BufferTris-citrate/phosphate buffer containing 0.09% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 1-2 µg/ChIP Fig 1, 2
Western blot 1:1,000 Fig 3
IP 6 µg per IP Fig 4

* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per ChIP.

1Manufactured by Bethyl Laboratories, Inc., Texas, USA

  • Validation data
    CBX8 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8
    ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A. CBX8 Antibody ChIP-seq Grade
    B.CBX8 Antibody for ChIP-seq
    C.CBX8 Antibody for ChIP-seq assay
    D.CBX8 Antibody validated in ChIP-seq
    E.CBX8 Antibody ChIP-seq Grade

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8
    ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.

    CBX8 Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8
    Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    CBX8 Antibody validated in Immunoprecipitation

    Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500.

  • Target Description

    CBX8 (UniProtKB/Swiss-Prot entry Q9HC52) is a component of the polycomb group PRC1-like multiprotein complex. This complex is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. It mediates monoubiquitination of histone H2A Lys-119, which leads to recruitment of PRC2 and methylation of H3K27 and H3K9.

  •  Applications
    ChIP-qPCR (ab)
    Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunoprecipitation Read more
    ChIP-seq (ab)
    Read more
  •  Documents
    CBX8 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human CBX8 (chromobox 8), using a synthetic peptide ...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CBX8 Antibody - ChIP-seq Grade (Diagenode Cat# C15410333 Lot# A300-882A2). Click here to copy to clipboard.

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