Request a quote for a bulk order for MYH11 polyclonal antibody - Classic. Please fill out the form here below. Your local sales account manager will get in touch with you
shortly and send you a quotation based on your requirements.
Polyclonal antibody raised in rabbit against human MYH11 (Myosin, Heavy Chain 11) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.
Lot
A1379-001
Concentration
not determined
Species reactivity
Human
Type
Polyclonal
Purity
Whole antiserum
Host
Rabbit
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11 ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.
A. B. C. D.
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11 ChIP was performed as described above. The IP’d DNA from 6 ChIP’s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.
Figure 3. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.
Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11 Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
Learn more about: Load... Read more
To ensure you see the information most relevant to you, please select your country.
Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.