Diagenode

>>>   Click for Diagenode’s approach to COVID-19

YY1 Antibody - ChIP-seq Grade

Catalog Number
Format
Price
C15410345
50 μg
$325.00
  Bulk order
Other format



Other names: INO80S, NF-E1, YY-1, Yin-Yang-1, UCRBP

Polyclonal antibody raised in rabbit against human Transcription factor YY1, using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.

LotA2649-0040
Concentration2.3 μg/μl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody in PBS containing 0.05% azide.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 1 μg/ChIP Fig 1, 2
ELISA 1:10,000 - 1:100,000 Fig 3
WB 1:1,000 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation data

    YY1 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against YY1
    ChIP assays were performed using K562 cells, the Diagenode antibody against YY1 (Cat. No. C15410345) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the FBXL18 and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    YY1 Antibody ChIP-seq Grade

    YY1 Antibody for ChIP-seq

    YY1 Antibody for ChIP-seq assay

    YY1 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against YY1
    ChIP was performed with 1 μg of the Diagenode antibody against YY1 (Cat. No. C15410345) on sheared chromatin from 4,000,000 K562 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the FBXL18 and EIF2S3 positive control genes (figure 2C and D).

    YY1 Antibody ELISA validation

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against YY1 (Cat. No. C15410345). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.

    YY1 Antibody validated in Western Blot

    Figure 4. Western blot analysis using the Diagenode antibody directed against YY1
    Whole cell extracts from K562 cells were analysed by Western blot using the Diagenode antibody against YY1 (Cat. No. C15410345) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Target Description

    YY1 (UniProtKB/Swiss-Prot entry P25490) is a ubiquitously distributed transcription factor which can both activate or repress a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Whether it activates or represses transcription depends upon the context in which it binds. YY1 is thought to direct histone deacetylases and histone acetyltransferases to the promoters of it’s target genes in order to activate or repress transcription, thus implicating histone modification in the function of YY1.

  •  Applications
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    YY1 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human Transcription factor YY1, using two synthetic ...
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: YY1 Antibody - ChIP-seq Grade (Diagenode Cat# C15410345 Lot# A2649-0040). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics