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JMJD2a Antibody - ChIP-seq Grade

Catalog Number
Format
Price
C15410332
100 μl
$355.00
  Bulk order



Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a synthetic peptide containing a sequence from the C-terminal part of the protein.

LotA300-861A1
Concentration100 μl
Species reactivityHuman, mouse
TypePolyclonal ChIP grade, ChIP-seq grade
PurityAffinity purified
HostRabbit
Storage ConditionsStore at 4°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μg/ChIP Fig 1, 2
Western Blotting 1:500 Fig 3
IP 3 μg per IP Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation data

    JMJD2a Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a
    ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A.JMJD2a Antibody ChIP-seq Grade
    B.JMJD2a Antibody for ChIP-seq assay
    C.JMJD2a Antibody validated in ChIP-seq
    D.JMJD2a Antibody for ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a
    ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    JMJD2a Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a
    Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    JMJD2a Antibody validated in Immunoprecipitation

    Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200.

       Manufactured by Bethyl Laboratories, Inc., Texas, USA
  • Target Description

    JMJD2a (UniProtKB/Swiss-Prot entry O75164), belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2a specifically demethylates the trimethylated K9 and K36 of histone H3, thereby converting these lysines to the dimethylated form. It has no activity towards H3K4, H3K27 and H4K20, or to the mono- and dimethylated H3K9 and H3K36. JMJD2a plays a role in the transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.

  •  Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
    ChIP-qPCR (ab)
    Read more
    ChIP-seq (ab)
    Read more
  •  Documents
    JMJD2a polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: JMJD2a Antibody - ChIP-seq Grade (Diagenode Cat# C15410332 Lot# A300-861A1). Click here to copy to clipboard.

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