Diagenode

CHD7 polyclonal antibody

Antibody ChIP-seq grade icon
Catalog Number
Format
Price
C15410340
100 μl
$305.00
  Bulk order

Polyclonal antibody raised in rabbit against human CHD7 (Chromodomain Helicase DNA Binding Protein 1), using a synthetic peptide containing a sequence from the C-terminal part of the protein1.

LotA301-223A1
Concentration0.2 μg/μl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody in TBS containing 0.1% BSA and 0.09% azide.
HostRabbit
Storage ConditionsStore at 4°C.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 4 μg/ChIP Fig 1, 2
Western Blotting 1:1,000 Fig 3
Immunofluorescence 1:500 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

1Manufactured by Bethyl Laboratories, Inc., Texas, USA

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7
    ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq A

    ChIP-seq B

    ChIP-seq C

    ChIP-seq D

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7
    ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).

    WB

    Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7
    Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IP

    Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.

  •  Applications
    ChIP-seq (ab)
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    ChIP-qPCR (ab)
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    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  •  Documents
    CHD7 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human CHD7 (Chromodomain Helicase DNA Binding Protei...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CHD7 polyclonal antibody (Diagenode Cat# C15410340 Lot# A301-223A1). Click here to copy to clipboard.

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