Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7
ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).