Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC6
ChIP was performed with the Diagenode antibody against HDAC6 (cat. No. C15410362) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 0.5, 1, 3 and 6 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the GAPDH and EIF4A2 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).