Diagenode

HDAC6 polyclonal antibody

カタログ番号
フォーマット
価格
C15410362
50 µg
$315.00
  Bulk order

Polyclonal antibody raised in rabbit against human HDAC6 (Histone deacetylase 6), using two KLH-conjugated synthetic peptides from the N-terminal and the central part of the protein, respectively.

LotA2850P
Concentration0.5 µg/µl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq* 3 µg/ChIP Fig 1, 2
ELISA 1:1,000 Fig 3
Western Blotting Not recommended
  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against HDAC6
    ChIP was performed with the Diagenode antibody against HDAC6 (cat. No. C15410362) on sheared chromatin from 4,000,000 HeLa cells with the iDeal ChIP-seq kit for TF’s. An antibody titration consisting of 0.5, 1, 3 and 6 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers specific for the GAPDH and EIF4A2 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq

    ChIP-seq

    ChIP-seq

    ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against HDAC6
    ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 3 µg of the Diagenode antibody against HDAC6 (cat. No. C15410362) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 300 kb region of chromosome 1 (figure 2A and B) and in two genomic regions surrounding the EIF2S3 and GAPDH positive control genes, respectively (figure 2C and D).

    ELISA

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC6 (cat. No. C15410362). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:11,400.

  •  実験手法
  •  資料
    HDAC6 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human HDAC6 (Histone deacetylase 6), using two KLH-c...
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  •  出版物

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イベント

  • A Century of Genetics
    Edinburgh
    Nov 13-Nov 15, 2019
  • EMEA User Group Meeting - PacBio
    Barceló Hotel, Milan, Italy
    Nov 14-Nov 15, 2019
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