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KAT2B Antibody - ChIP-seq Grade

Catalog Number
Format
Price
C15410335
100 μl
$355.00
  Bulk order



Polyclonal antibody raised in rabbit against human KAT2B (Lysine Acetyltransferase 2B), using a synthetic peptide containing a sequence from the C-terminal part of the protein.

LotA301-666A2
Concentration100 µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
Storage ConditionsStore at 4°C
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 2 μg/ChIP Fig 1, 2
Western Blotting 1:1,000 Fig 3

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation data

    ChIPKAT2B Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against KAT2B
    ChIP assays were performed using K562 cells, the Diagenode antibody against KAT2B (Cat. No. C15410335) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    KAT2B Antibody ChIP-seq Grade

    ChIP-seqKAT2B Antibody for ChIP-seq

    KAT2B Antibody for ChIP-seq assay

    ChIP-seqKAT2B Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B
    ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 μg of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).

    KAT2B Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B
    Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Target Description

    KAT2B (UniProtKB/Swiss-Prot entry Q92831) functions as a histone acetyltransferase, thereby promoting transcriptional activation. KAT2B binds with CBP and p300 where it competes with the adenoviral oncoprotein E1A for binding sites and as such counteracts the mitogenic activity of E1A. KAT2B also acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers.

  •  Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
    ChIP-seq (ab)
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  •  Documents
    KAT2B polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human KAT2B (Lysine Acetyltransferase 2B), using a s...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: KAT2B Antibody - ChIP-seq Grade (Diagenode Cat# C15410335 Lot# A301-666A2). Click here to copy to clipboard.

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