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Figure 1. ChIP results obtained with the Diagenode antibody directed against KAT2B ChIP assays were performed using K562 cells, the Diagenode antibody against KAT2B (Cat. No. C15410335) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against KAT2B ChIP was performed on sheared chromatin from 4 million KAT2B cells using 2 μg of the Diagenode antibody against KAT2B (cat. No. C15410335) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 500 kb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and EIF4A2 positive control genes (fig 2C and D).
Figure 3. Western blot analysis using the Diagenode antibody directed against KAT2B Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against KAT2B (Cat. No. C15410335) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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