Diagenode

MLL1 polyclonal antibody

Antibody ChIP-seq grade icon
Catalog Number
Format
Price
C15310264
100 µl
$305.00
  Bulk order
Other format

Other names: KMT2A, ALL1, CXXC7, TRX1, MLL, HRX, HTRX, HTRX1, WDSTS

Polyclonal antibody raised in rabbit against human MLL1 (Mixed-Lineage Leukemia) using two KLH-conjugated synthetic peptides containing a sequence from the central region of the protein.

LotA1542-001
ConcentrationNot determined
Species reactivityHuman. Other species: not tested.
TypePolyclonal ChIP grade, ChIP-seq grade
PurityWhole antiserum from rabbit containing 0.05% azide.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution/amount References
ChIP * 2 µl/ChIP Fig 1, 2
ELISA 1:100 - 1:1,000 Fig 3

*Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 µl per IP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against MLL1
    ChIP was performed on MV4-11 cells using the Diagenode antibody against MLL1 (Cat. No. C15310264). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).

    ChIP-seq A

    ChIP-seq B

    ChIP-seq C

    ChIP-seq D

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1
    ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).

    ELISA

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.

  •  Applications
  •  Documents
    MLL1 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human MLL1 (Mixed-Lineage Leukemia) using two KLH-co...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MLL1 polyclonal antibody (Diagenode Cat# C15310264 Lot# A1542-001). Click here to copy to clipboard.

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