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Figure 1. ChIP results obtained with the Diagenode antibody directed against MLL1 ChIP was performed on MV4-11 cells using the Diagenode antibody against MLL1 (Cat. No. C15310264). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1 ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).
Figure 3. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.