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MLL1 Antibody - ChIP-seq Grade (sample size)

Catalog Number
20 µl
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Polyclonal antibody raised in rabbit against human MLL1 (Mixed-Lineage Leukemia) using two KLH-conjugated synthetic peptides containing a sequence from the central region of the protein.

ConcentrationNot determined
Species reactivityHuman. Other species not tested.
TypePolyclonal ChIP grade, ChIP-seq grade
PurityWhole antiserum from rabbit containing 0.05% azide.
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution/amount References
ChIP/ChIP-seq * 2 µl/ChIP Fig 1, 2
ELISA 1:100 - 1:1,000 Fig 3

*Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 µl per IP.

  • Validation data

    MLL1 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against MLL1
    ChIP was performed on MV4-11 cells using the Diagenode antibody against MLL1 (Cat. No. C15310264). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).

    MLL1 Antibody ChIP-seq Grade

    MLL1 Antibody for ChIP-seq

    MLL1 Antibody for ChIP-seq assay

    MLL1 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1
    ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).

    MLL1 Antibody ELISA validation

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.

  • Target Description

    MLL1 (UniProtKB/Swiss-Prot entry Q03164) functions as a lysine methyltransferase responsible for the methylation of H3K4 which is associated with epigenetic transcriptional activation. MLL1 regulates the transcription of specific target genes, including many of the HOX genes and plays an essential role in regulating gene expression during early development and hematopoiesis. It also plays a role in the control of circadian gene expression. Mll1 is involved in several translocations which cause certain acute lymphoid leukemias and acute myeloid leukemias.

  •  Applications
  •  Documents
    MLL1 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human MLL1 (Mixed-Lineage Leukemia) using two KLH-co...
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MLL1 Antibody - ChIP-seq Grade (sample size) (Diagenode Cat# C15310264-20 Lot# A1542-001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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