Shearing: You need fragments that are optimally-sized for effective immunoprecipitations and accurate downstream analysis sush as library prep.
Bioruptor Pico for controlled shearing to achieve the right size range you need for ChIP
Chromatin Shearing Optimization Kits
Fragment size requirements
Fragments between 100-500 bp are suitable for the majority of ChIP experiments. Many ChIP-seq experiments are commonly performed with chromatin in the 100-800 bp range. Generally, a tighter fragment distribution is recommended for ChIP-seq than for ChIP-qPCR. Typically, 100-300 bp is compatible with histone ChIP-seq while a larger range is more suitable for transcriptional factors, indirectly-bound proteins, and distant interacting proteins.
Achieving best fragmentation
Use the Bioruptor Pico: More than 8,000 peer-reviewed scientific papers cite the Bioruptor. The range of chromatin size can be easily fine-tuned with the Bioruptor Pico. The Bioruptor Pico delivers high performance ultrasound in with the needed temperature control, allowing optimal shearing efficency. Remember to use the appropriate tubes (available from Diagenode).
Use the Chromatin Shearing Optimization Kits: It is important to establish optimal conditions to shear crosslinked chromatin (protein-DNA). Usually this process requires optimizing SDS concentration, which is laborious. With the Shearing Optimization Kit, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS, optimized for both transcription factors and histones.
Read the full Chromatin Shearing Guide: you'll know everything and more about best shearing strategy.