Diagenode

iDeal ChIP-qPCR Kit

Catalog Number
Format
Price
C01010180
24 rxns
$495.00

Diagenode’s iDeal ChIP-qPCR kit is a highly optimized and validated solution for ChIP-qPCR assays for either histones or transcription factors. The iDeal ChIP-qPCR kit, in conjunction with our validated ChIP-grade antibodies, provides excellent, reproducible results. The complete kit contains everything you need for start-to-finish ChIP including all validated buffers and reagents for chromatin shearing, immunoprecipitation, and DNA isolation for exceptional ChIP-qPCR results.

The iDeal ChIP-qPCR kit uses a unique and fast method for DNA isolation and decrosslinking within 30 minutes compared to the standard 4 hours. Overall, easy to use and rapid protocol allows to receive the results within 20 hours with 4 hours hands-on time. 

Recommended amount of material per IP using the iDeal ChIP-qPCR kit:

Histones

Transcription factors

Cells

100,000 to 1 million cells 

4 million cells

Tissue

1.5 mg to 5 mg

30 mg

  • Characteristics
    • Fast and highly optimized protocol for ChIP-qPCR from cells and tissues
    • Easy ChIP made faster and more reproducible with magnetic beads
    • High yields with excellent specificity and sensitivity due to combination of Diagenode ChIP-grade antibodies
    • Eluted DNA suitable for qPCR analysis

    Optimized protocol for reproducible ChIP-qPCR results

    Ideal for histone and non-histone proteins – Use 1 million cells for histone marks or 4 million cells for transcription factors. The protocol allows the use of fewer cells per IP. The minimum number of cells will depend on the abundance of the protein in your sample.

    Suitable for cells and for tissues samples – For tissues, use 7 mg per IP for histone marks or 30 mg per IP for transcription factors. The protocol allows the use of less tissue per IP. The minimum amount of tissue will depend on the abundance of the protein in your sample.

    Chromatin immunoprecipitation analysis using H3K4me3 (A) and CTCF antibodies (B)
    ChIP was performed on human HeLa cells using the H3K4me3 (Cat. No. C15410003) and CTCF (Cat. No. C15410210) antibodies. IgG was used as a negative control. The IP’d DNA was analyzed by qPCR with the following primer sets: EIF4A2, used as a positive control, and THS2B and Myoglobin exon 2, used as negative controls for H3K4me3. H19 imprinting control region and GAPDH intron 8, used as positive controls, and Myoglobin exon 2, used as a negative control for CTCF. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  • Additional solutions compatible with iDeal ChIP-qPCR Kit

    The Chromatin shearing optimization kit – Low SDS (iDeal Kit for TFs) is the kit compatible with the iDeal ChIP-qPCR kit, recommended for the optimization of chromatin shearing, a critical step for ChIP.

    ChIP Cross-link Gold should be used in combination with formaldehyde when working with higher order and/or dynamic interactions, for efficient protein-protein fixation.

    Highly specific ChIP-grade antibodies, provided with batch-specific validation data.

    Check out the list of available Primer pairs designed for high specificity to specific genomic regions.

    Using our IP-Star Compact, please check out the automated version of the kit.

  •  Applications
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
    Chromatin Analysis
    Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichme... Read more
  •  Documents
    iDeal ChIP qPCR Kit manual MANUAL
    Diagenode’s iDeal ChIP-qPCR Kit is a highly optimized solution for ChIP-qPCR assays. Two ve...
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    iDeal ChIP qPCR Kitマニュアル マニュアル
    DiagenodeのiDeal ChIP-qPCRキットは、ChIP-qPCRアッセイ用に最適化されたソリューションです。 このキットは優れた特異性と感度で高い収量を可能にします。 私たちの高度...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: iDeal ChIP-qPCR Kit (Diagenode Cat# C01010180). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Elevated cyclic-AMP represses expression of exchange protein activated by cAMP (EPAC1) by inhibiting YAP-TEAD activity and HDAC-mediated histone deacetylation.
    Ebrahimighaei R, McNeill MC, Smith SA, Wray JP, Ford KL, Newby AC, Bond M
    Ligand-induced activation of Exchange Protein Activated by cAMP-1 (EPAC1) is implicated in numerous physiological and pathological processes, including cardiac fibrosis where changes in EPAC1 expression have been detected. However, little is known about how EPAC1 expression is regulated. Therefore, we investigated r...

    Adolescent social isolation affects parvalbumin expression in the medial prefrontal cortex in the MAM-E17 model of schizophrenia.
    Maćkowiak M, Latusz J, Głowacka U, Bator E, Bilecki W
    Altered parvalbumin (PV) expression is observed in the prefrontal cortex of subjects with schizophrenia. Environmental context, particularly during adolescence, might regulate PV expression. In the present study, we investigated the effect of adolescent social isolation (SI) on PV expression in the medial prefrontal...

    Enhancers in the Peril lincRNA locus regulate distant but not local genes.
    Groff AF, Barutcu AR, Lewandowski JP, Rinn JL
    BACKGROUND: Recently, it has become clear that some promoters function as long-range regulators of gene expression. However, direct and quantitative assessment of enhancer activity at long intergenic noncoding RNA (lincRNA) or mRNA gene bodies has not been performed. To unbiasedly assess the enhancer capacity across...

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