Diagenode

iDeal ChIP-qPCR kit

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Catalog Number
Format
Price
C01010180
24 rxns
$480.00

Diagenode’s iDeal ChIP-qPCR kit is a highly optimized solution for ChIP-qPCR assays. The kit provides high yields with excellent specificity and sensitivity. The iDeal ChIP-qPCR kit used with our highly validated ChIP-grade antibodies provides you with excellent, reproducible results from each experiment.

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  • Characteristics

    Optimized protocol for reproducible ChIP-qPCR results

    Ideal for histone and non-histone proteins – Use 1 million cells for histone marks or 4 million cells for transcription factors. The protocol allows the use of fewer cells per IP. The minimum number of cells will depend on the abundance of the protein in your sample.

    Suitable for cells and for tissues samples – For tissues, use 7 mg per IP for histone marks or 30 mg per IP for transcription factors. The protocol allows the use of less tissue per IP. The minimum amount of tissue will depend on the abundance of the protein in your sample.

    Chromatin immunoprecipitation analysis using H3K4me4 (A) and CTCF antibodies (B)
    ChIP was performed on human HeLa cells using the H3K4me4 (Cat. No. C15410003) and CTCF (Cat. No. C15410210) antibodies. IgG was used as a negative control. The IP’d DNA was analyzed by qPCR with the following primer sets: EIF4A2, used as a positive control, and THS2B and Myoglobin exon 2, used as negative controls for H3K4me3. H19 imprinting control region and GAPDH intron 8, used as positive controls, and Myoglobin exon 2, used as a negative control for CTCF. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  • Applications
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
    Epigenetics Chromatin Analysis
    Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichme... Read more
  • Documents
    iDeal ChIP qPCR Kit manual MANUAL
    Diagenode’s iDeal ChIP-qPCR kit is a highly optimized solution for ChIP-qPCR assays. The ki...
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  • Publications

    How to properly cite this product in your work

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