Diagenode

Chromatin EasyShear Kit - Low SDS

Catalog Number
Format
Price
C01020013
4 chrom. prep./ 24 rxns
$345.00

Previous name of the kit: Chromatin shearing optimization kitLow SDS (iDeal Kit for TFs)

A high quality chromatin preparation is very complex and requires a lot of optimization. Chromatin EasyShear Kit – Low SDS is an optimized solution for efficient chromatin preparation prior to ChIP. The protocol, buffers composition, SDS concentration (0.2%) is optimized for the preparation of chromatin prior to ChIP on transcription factors and other non-histone proteins. The kit has been validated with the Bioruptor ultrasonicator for efficient chromatin shearing, leading to chromatin fragments suitable for ChIP with the preserved epitopes. The kit is validated for cells, tissues and FFPE samples.

Check out all of the Chromatin EasyShear Kits.

Guide for the optimal chromatin preparation using Chromatin EasyShear Kits - Read more

  • Characteristics
    • Highly optimized protocol for chromatin preparation prior to ChIP on transcription factors and non-histone proteins
    • SDS concentration optimized for the workflow for TFs
    • Validated for cells, tissue and FFPE samples
    • Preserves the epitopes
    • Validated with the Bioruptor ultrasonicator
    • Quality of chromatin sample confirmed by ChIP-seq

    Figure 1. Optimal chromatin shearing profile
    HeLa cells were fixed with formaldehyde for 10 min and chromatin was prepared according to Diagenode’s Chromatin EasyShear Kit - Low SDS (Cat. No. C01020013). Samples were sonicated for 5-10-15 cycles of 30” ON/30” OFF as indicated with Bioruptor Pico using 1.5 ml Bioruptor microtubes with caps (Cat. No. C30010016) followed by de-crosslinking and DNA purification. The fragment size was assessed using agarose gel electrophoresis. A 100 bp ladder was loaded as the size standard.

    Figure 2. Chromatin precipitation
    Sheared chromatin (obtained with the Chromatin EasyShear Kit – Low SDS) has been used for immunoprecipitation with CTCF and IgG (negative control) antibodies. Quantitative PCR was performed with positive (H19) and negative (Myoglobine exon 2) control regions. The Figure 2 shows the recovery expressed as % of input (the relative amount of immunoprecipitated DNA compared to input DNA (panel A) and as enrichment fold of positive locus over negative (panel B).

  • Cells tested

    The Chromatin EasyShear Kit is compatible with a broad variety of cell lines, tissues and species – some examples are shown below. Other species / cell lines / tissues can be used with this kit.

    Cell lines:

    • Human: A549, A673, BT-549, CD4 T, HCC1806, HeLa, HepG2, HFF, HK-GFP-MR, ILC, K562, KYSE-180, LapC4, M14, MCF7, MDA-MB-231, MDA-MB-436, RDES, SKNO1, VCaP, U2-OS, ZR-75-1
    • Mouse: ESC, NPCs, BZ, GT1-7, acinar cells, HSPCs, Th2 cells, keratinocytes
    • Cattle: pbMEC, MAC-T
    • Other cell lines / species: compatible, not tested

    Tissues:

    • Mouse: kidney, heart, brain, iris, liver, limbs from E10.5 embryos
    • Horse: liver, brain, heart, lung, skeletal muscle, lamina, ovary
    • Other tissues: compatible, not tested

    Did you use the iDeal ChIP-seq for Transcription Factors Kit on other cell line / tissue / species? Let us know!

  •  Documents
    Chromatin Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    Download
    Chromatin EasyShear Kit - Low SDS MANUAL
    Old name: Chromatin shearing optimization kit - Low SDS (for Transcription Factors) ...
    Download
  •  Safety sheets
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Chromatin EasyShear Kit - Low SDS (Diagenode Cat# C01020013). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Nuclear lamin A/C phosphorylation by loss of androgen receptor leads to cancer-associated fibroblast activation
    Ghosh S. et al.
    Alterations in nuclear structure and function are hallmarks of cancer cells. Little is known about these changes in Cancer-Associated Fibroblasts (CAFs), crucial components of the tumor microenvironment. Loss of the androgen receptor (AR) in human dermal fibroblasts (HDFs), which triggers early steps of CAF act...

    Loss of epigenetic regulation disrupts lineage integrity, inducesaberrant alveogenesis and promotes breast cancer.
    Langille E. et al.
    Systematically investigating the scores of genes mutated in cancer and discerning disease drivers from inconsequential bystanders is a prerequisite for Precision Medicine but remains challenging. Here, we developed a somatic CRISPR/Cas9 mutagenesis screen to study 215 recurrent 'long-tail' breast cancer genes, which...

    X-linked myotubular myopathy is associated with epigenetic alterationsand is ameliorated by HDAC inhibition.
    Volpatti Jonathan R et al.
    X-linked myotubular myopathy (XLMTM) is a fatal neuromuscular disorder caused by loss of function mutations in MTM1. At present, there are no directed therapies for XLMTM, and incomplete understanding of disease pathomechanisms. To address these knowledge gaps, we performed a drug screen in mtm1 mutant zebrafish and...

    EHF is a novel regulator of cellular redox metabolism and predictspatient prognosis in HNSCC.
    Oyelakin A. et al.
    Head and Neck Squamous Cell Carcinoma (HNSCC) is a heterogeneous disease with relatively high morbidity and mortality rates. The lack of effective therapies, high recurrence rates and drug resistance driven in part, by tumor heterogeneity, contribute to the poor prognosis for patients diagnosed with this cancer. Thi...

    ZBTB11 dysfunction: spectrum of brain abnormalities, biochemicalsignature and cellular consequences.
    Sumathipala D. et al
    Bi-allelic pathogenic variants in ZBTB11 have been associated with Intellectual Developmental Disorder, autosomal recessive 69 (MRT69; OMIM 618383). We report five patients from three families with novel, bi-allelic variants in ZBTB11. We expand the clinical phenotype of MRT69, documenting varied severity of atrophy...

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