Diagenode

iDeal ChIP-qPCR kit

Catalog Number
Format
Price
C01010180
24 rxns
$515.00

DiagenodeのiDealChIP-qPCR kitは、優れた特異性と感度で高い収量を可能にするChIP-qPCR アッセイ用に最適化されたソリューションです。高度に検証されたChIPグレード抗体と一緒に使用することで、優れた再現性のある結果を提供します。

  • 特徴
    • Fast and highly optimized protocol for ChIP-qPCR from cells and tissues
    • Easy ChIP made faster and more reproducible with magnetic beads
    • High yields with excellent specificity and sensitivity due to combination of Diagenode ChIP-grade antibodies
    • Eluted DNA suitable for qPCR analysis

    Optimized protocol for reproducible ChIP-qPCR results

    Ideal for histone and non-histone proteins – Use 1 million cells for histone marks or 4 million cells for transcription factors. The protocol allows the use of fewer cells per IP. The minimum number of cells will depend on the abundance of the protein in your sample.

    Suitable for cells and for tissues samples – For tissues, use 7 mg per IP for histone marks or 30 mg per IP for transcription factors. The protocol allows the use of less tissue per IP. The minimum amount of tissue will depend on the abundance of the protein in your sample.

    Chromatin immunoprecipitation analysis using H3K4me3 (A) and CTCF antibodies (B)
    ChIP was performed on human HeLa cells using the H3K4me3 (Cat. No. C15410003) and CTCF (Cat. No. C15410210) antibodies. IgG was used as a negative control. The IP’d DNA was analyzed by qPCR with the following primer sets: EIF4A2, used as a positive control, and THS2B and Myoglobin exon 2, used as negative controls for H3K4me3. H19 imprinting control region and GAPDH intron 8, used as positive controls, and Myoglobin exon 2, used as a negative control for CTCF. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

  •  資料
    iDeal ChIP qPCR Kit manual MANUAL
    Diagenode’s iDeal ChIP-qPCR Kit is a highly optimized solution for ChIP-qPCR assays. Two ve...
    Download
  •  Safety sheets
    iDeal ChIP-qPCR Kit SDS GB en Download
    iDeal ChIP-qPCR Kit SDS US en Download
    iDeal ChIP-qPCR Kit SDS BE nl Download
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    iDeal ChIP-qPCR Kit SDS ES es Download
    iDeal ChIP-qPCR Kit SDS JP ja Download
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: iDeal ChIP-qPCR kit (Diagenode Cat# C01010180). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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    Zhu, Zifu and Ma, Li
    Postoperative cognitive dysfunction (POCD) is a normal condition that develops after surgery with anesthesia, leading to deterioration of cognitive functions. However, the mechanism of POCD still remains unknown. To elucidate the POCD molecular mechanism, sevoflurane was employed in the present study to generate neu...

    Regulatory interplay between Vav1, Syk and β-catenin occurs in lungcancer cells.
    Boudria Rofia et al.
    Vav1 exhibits two signal transducing properties as an adaptor protein and a regulator of cytoskeleton organization through its Guanine nucleotide Exchange Factor module. Although the expression of Vav1 is restricted to the hematopoietic lineage, its ectopic expression has been unraveled in a number of solid tumors. ...

    Epigenetic remodelling of enhancers in response to estrogen deprivationand re-stimulation.
    Sklias Athena et al.
    Estrogen hormones are implicated in a majority of breast cancers and estrogen receptor alpha (ER), the main nuclear factor mediating estrogen signaling, orchestrates a complex molecular circuitry that is not yet fully elucidated. Here, we investigated genome-wide DNA methylation, histone acetylation and transcriptio...

    Enhanced targeted DNA methylation of the CMV and endogenous promoterswith dCas9-DNMT3A3L entails distinct subsequent histonemodification changes in CHO cells.
    Marx Nicolas et al.
    With the emergence of new CRISPR/dCas9 tools that enable site specific modulation of DNA methylation and histone modifications, more detailed investigations of the contribution of epigenetic regulation to the precise phenotype of cells in culture, including recombinant production subclones, is now possible. These al...

    FOS licenses early events in stem cell activation driving skeletal muscleregeneration.
    Almada, Albert E. et al.
    Muscle satellite cells (SCs) are a quiescent (non-proliferative) stem cell population in uninjured skeletal muscle. Although SCs have been investigated for nearly 60 years, the molecular drivers that transform quiescent SCs into the rapidly dividing (activated) stem/progenitor cells that mediate muscle repair after ...

    The transcription factor scleraxis differentially regulates gene expressionin tenocytes isolated at different developmental stages.
    Paterson, YZ and Evans, N and Kan, S and Cribbs, A and Henson, FMD andGuest, DJ
    The transcription factor scleraxis (SCX) is expressed throughout tendon development and plays a key role in directing tendon wound healing. However, little is known regarding its role in fetal or young postnatal tendons, stages in development that are known for their enhanced regenerative capabilities. Here we used ...

    TET-Mediated Hypermethylation Primes SDH-Deficient Cells for HIF2α-Driven Mesenchymal Transition.
    Morin A, Goncalves J, Moog S, Castro-Vega LJ, Job S, Buffet A, Fontenille MJ, Woszczyk J, Gimenez-Roqueplo AP, Letouzé E, Favier J
    Loss-of-function mutations in the SDHB subunit of succinate dehydrogenase predispose patients to aggressive tumors characterized by pseudohypoxic and hypermethylator phenotypes. The mechanisms leading to DNA hypermethylation and its contribution to SDH-deficient cancers remain undemonstrated. We examine th...

    The nuclear hypoxia-regulated NLUCAT1 long non-coding RNA contributes to an aggressive phenotype in lung adenocarcinoma through regulation of oxidative stress.
    Moreno Leon L, Gautier M, Allan R, Ilié M, Nottet N, Pons N, Paquet A, Lebrigand K, Truchi M, Fassy J, Magnone V, Kinnebrew G, Radovich M, Cheok MH, Barbry P, Vassaux G, Marquette CH, Ponzio G, Ivan M, Pottier N, Hofman P, Mari B, Rezzonico R
    Lung cancer is the leading cause of cancer death worldwide, with poor prognosis and a high rate of recurrence despite early surgical removal. Hypoxic regions within tumors represent sources of aggressiveness and resistance to therapy. Although long non-coding RNAs (lncRNAs) are increasingly recognized as major gene ...

    Elevated cyclic-AMP represses expression of exchange protein activated by cAMP (EPAC1) by inhibiting YAP-TEAD activity and HDAC-mediated histone deacetylation.
    Ebrahimighaei R, McNeill MC, Smith SA, Wray JP, Ford KL, Newby AC, Bond M
    Ligand-induced activation of Exchange Protein Activated by cAMP-1 (EPAC1) is implicated in numerous physiological and pathological processes, including cardiac fibrosis where changes in EPAC1 expression have been detected. However, little is known about how EPAC1 expression is regulated. Therefore, we investigated r...

    Adolescent social isolation affects parvalbumin expression in the medial prefrontal cortex in the MAM-E17 model of schizophrenia.
    Maćkowiak M, Latusz J, Głowacka U, Bator E, Bilecki W
    Altered parvalbumin (PV) expression is observed in the prefrontal cortex of subjects with schizophrenia. Environmental context, particularly during adolescence, might regulate PV expression. In the present study, we investigated the effect of adolescent social isolation (SI) on PV expression in the medial prefrontal...

    Enhancers in the Peril lincRNA locus regulate distant but not local genes.
    Groff AF, Barutcu AR, Lewandowski JP, Rinn JL
    BACKGROUND: Recently, it has become clear that some promoters function as long-range regulators of gene expression. However, direct and quantitative assessment of enhancer activity at long intergenic noncoding RNA (lincRNA) or mRNA gene bodies has not been performed. To unbiasedly assess the enhancer capacity across...

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